http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Simple Maturation of Direct-Converted Hepatocytes Derived from Fibroblasts
조영덕,윤상태,강교진,김요한,이승범,서대관,류기영,정재민,최동호 한국조직공학과 재생의학회 2017 조직공학과 재생의학 Vol.14 No.5
Target cells differentiation techniques from stem cells are developed rapidly. Recently, direct conversion techniques are introduced in various categories. Unlike pluripotent stem cells, this technique enables direct differentiation into the other cell types such as neurons, cardiomyocytes, insulin-producing cells, and hepatocytes without going through the pluripotent stage. However, the function of these converted cells reserve an immature phenotype. Therefore, we modified the culture conditions of mouse direct converted hepatocytes (miHeps) to mature fetal characteristics, such as higher AFP and lower albumin (ALB) expression than primary hepatocytes. First, we generate miHeps from mouse embryonic fibroblasts (MEFs) with two transcription factors HNF4a and Foxa3. These cells indicate typical epithelial morphology and express hepatic proteins. To mature hepatic function, DMSO is treated during culture time for more than 7 days. After maturation, miHeps showed features of maturation such as exhibiting typical hepatocyte-like morphology, increased up-regulated ALB and CYP enzyme gene expression, down-regulated AFP expressions, and acquired hepatic function over time. Thus, our data provides a simple method to mature direct converted hepatocytes functionally and these cells enable them to move closer to generating functional hepatocytes.
조영덕,박성준,최성혁,윤영훈,김정윤,이성우,임채승 대한외과학회 2017 Annals of Surgical Treatment and Research(ASRT) Vol.93 No.4
Purpose: Postcardiac arrest syndrome (PCAS) shares many features with sepsis including plasma cytokine elevation with dysregulation of cytokine production, and the presence of endotoxin in plasma. PCAS is closely related to ischemia-reperfusion injury. During ischemia-reperfusion injury, neutrophil, which is the first line of innate immunity, plays a major role. In this study, we investigated the inflammatory response of human neutrophils in an in vitro model which we simulated with hypoxia-normoxia and hypoxia-hyperoxia environments. Methods: After separation of neutrophils from the whole blood, they were divided into 3 experimental groups: normoxianormoxia, hypoxia-normoxia, and hypoxia-hyperoxia groups. The production of H2O2, the expression of Toll-like receptor 4 (TLR4) receptor, and the extent of apoptosis of the neutrophils were checked. Results: The in vitro hypoxia-normoxia and -hyperoxia models, which simulated the PCAS, showed initiation of the neutrophils’ inflammatory reaction by hypoxia insult. Lipopolysaccharide amplifies such inflammation; therefore, prevention of secondary infection may be critical in postresuscitation patients. Temporary hyperoxia following hypoxic insult showed no difference in inflammatory reaction compared with hypoxia-normoxia. Rather, temporary hyperoxia may suppress or minimize inflammation by attenuation of TLR4 receptor. Conclusion: It is well known that continuous hyperoxygenation after successful cardiac arrest harms patients, but temporary hyperoxygenation with 100% O2 in a clinical situation may be helpful
Inversion of the classical Radon transform on $\mathbb{Z}^n_p$
조영덕,현종윤,문성환 대한수학회 2018 대한수학회보 Vol.55 No.6
The Radon transform introduced by J. Radon in 1917 is the integral transform which is widely applicable to tomography. Here we study the discrete version of the Radon transform. More precisely, when $\mathcal{C}(\mathbb{Z}^n_p)$ is the set of complex-valued functions on $\mathbb{Z}^n_p$. We completely determine the subset of $\mathcal{C}(\mathbb{Z}^n_p)$ whose elements can be recovered from its Radon transform on $\mathbb{Z}^n_p$.
조영덕,최성혁,윤영훈,박상민,김정윤,임채승 대한수혈학회 2012 大韓輸血學會誌 Vol.23 No.2
Background:Potassium, the most common cation in the intracellular space, plays a critical role in our physiology. Potassium imbalance may cause life-threatening problems, ranging from general weakness to cardiac arrest due to ventricular fibrillation. For emergency physicians, detection of such derangement within a short period of time is of critical importance. In this study, we wanted to determine whether analysis of whole blood samples can be used as a screening tool for potassium imbalance by comparative analysis of whole blood and serum samples. Methods:Two samples were drawn from 227 patients. The whole blood sample was taken from the radial artery and contained in a commercially available arterial blood collection syringe with a lithium-heparin coating. The serum sample was contained in a commercially available vacuum bottle in a non-additive silicone coated tube and transported to the laboratory. The study population was divided into three groups, patients with normal whole blood potassium, patients with decreased whole blood potassium, and patients with elevated whole blood potassium. Potassium levels for each group were coupled with serum potassium levels and compared. Results:No significant difference in potassium values was observed between whole blood and serum samples (P<0.05). Strong associations were observed among the three groups (normal range, hypokalemia, and hyperkalemia group). Compared to the normal group (r=0.851), the hyperkalemia group showed a stronger association between variables (r=0.897), and the hypokalemia group showed a weaker association (r=0.760). Their correlation coefficients were highly significant (P<0.05). Conclusion:Our study illustrates that point-of-care testing using whole blood with whole blood can be a reliable screening tool when treating patients with suspicious potassium abnormality, especially in hyperkalemia patients.
수술이 불가능한 간문부 담관암 환자에서의 광역동 치료 효과
조영덕 대한췌담도학회 2005 대한췌담도학회지 Vol.10 No.2
비록 광역동 치료가 고식적이고 담즙 배액관 삽입과 병행하여 시행하고 있으나 시술과 관련된 뚜렷한 합병증이 없고 반복적 시술이 가능하면 여러 연구에서 삶의 질 및 생존률의 향상이 있어 수술이 불가능한 담관암환자에서 광역동 치료는 뚜렷한 이득이 있는 것은 사실이다. 담관암의 특징이 담도를 따라 종양이 확산되는 특징이 있어 원격성 전이가 없는 Bismuth 3형, 4형에서 광역동 치료는 유용하며 신체적 또는 고령으로 인해 수술을 시행할 수 없는 Bismuth 1, 2형 그리고 하부 담관암환자에서도 광역동치료를 병행하는 것은 유용할 것으로 생각한다. 그러나 좀 더 많은 증례 수에서 전향적 비교 연구 및 장기간 추적 관찰이 광역동 치료의 역할을 규명하는데 필요할 것으로 생각한다.
담석을 동반한 간내 담관암과 담석을 동반하지 않은 담관암의 임상적 비교
조영덕 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1
Background/Aim: Hepatolithiasis are very common in Korea and hepatolithiasis is a risk factor for cholangiocarcinoma. The differences of clinical, laboratory or radiological features between intrahepatic cholangiocarcinoma associated with hepatolithiasis and cholaniocar-cinoma alone does not well known until now. Author performed this study to define the clinical, laboratory or radiological differences between intrahepatic cholangiocarcinoma associated with hepatolithiasis and cholangiocarcinoma alone. Methods: Sixty-one patients with intrahepatic cholangiocarcinoma associated with or without hepatolithiasis were analyzed retrospectively. There were 32 patients with cholangiocarcinoma associated with intraheaptic stones (group HC) and 29 cholangiocarcinoma patients without intraheaptic stones (group C). In group HC, there were 21 males and 11 females with a mean age of 63.4 years. Group C contained 17 males and 12 females with a mean age of 61.9 years. Results: Patients with cholangiocarcinoma associated with intrahepatic stones had a significantly longer duration of symptoms, a higher frequency of biliary surgery than cholangiocarcinoma alone. In group HC, the main symptoms were abdominal pain, fever, chilling, and jaundice. In group C, the main symptoms were abdominal pain and jaundice. Concordance rate of location of stones and tumor was closely related in 96% of patients with cholangiocarcinoma and hepatolithiasis. Segmental stricture and dilated intrahepatic ducts were the most common radiological features of both groups. Tumor mass observed on the imaging study was encountered significantly frequent in the group C (82% vs. 53%, p<0.05). However, hepatic atrophy was noted more frequent in the group HC than group C (46% vs. 10%, p<0.05). There were no statistical differences between the two groups in the laboratory findings such as liver function test or tumor marker. Preoperative diagnostic rate of cholangiocarcinoma was lower in group HC than group C (78.2% vs. 96.6%). Conclusion: If bile duct stricture or hepatic atrophy are noted in patients with intrahepatic stones, active effort for detection of concomitant cholangiocarcinoma will be needed.