Effect of Ethanol on the Teratogenicity of Hypervitaminosis A in Rats

조대현,진강,조태순,Cho, Dae-Hyun,Chin, Kang,Cho, Tae-Soon The Korean Society of Food Hygiene and Safety 1990 한국식품위생안전성학회지 Vol.5 No.4

SPF wistar계 랫트를 이용하여 Vitamin A(VA)의 과잉 투여 ($40{\times}10^4IU/kg$)에 의한 최기형성 대하여 ethanol(E) 2g/kg을 임신 9일부터 임신 11일까지 3일간 경구로 병용 투여한 결과 다음과 같은 결과를 얻었다. 1.임신 모체의 영향에 있어서 $VA{\times}E$ 병용 투여군이 대조군 및 VA 단독투여군에 비해 유의한 체중증가의 억제를 보였다. 2. 태자 사망율은 VA+E 병용 투여군이 VA 단독 투여군에 비해 유의하게 증가되었고 (p<0.05), 태자의 평균 체중은 VA+E 병용 투여군에 대조군에 비해 유의하게 감소되었으며(p<0.01), VA 단독 투여군과의비교에서도 유의한 감소를 보였다. (p<0.05). 3. 외표기형의 발생율은 VA 단독투여군에 비해 VA+E 병용투여군이 증가되어 나타났다. 발현된 기형의 특징은 귀위치이상, 부이(복이), 안검개존, 소구증, 단악증, 구개열 등이다. 이상의 경과에서 Vitamin A의 과잉투여에 의한 최기형성에 ethanol이 상승적으로 작용함을 알 수 있다. The effects of ethanol (ET) on the teratogenicity of the fat soluble drug, Vita. min A (VA) were examined in SPF Wistar rats. VA and ET were orally administered with sonde. The drugs were administered for 3 days of day 9-11 of gestation. Four groups were made; G-I control (sesame oil + saline), G-II VA $40{\times}10^{4}$ (I.Ulkg/day), G-III ET 2 (g/kg/day), G-IV $40{\times}10^{4}$ + ET 2. Congenital malformations were found G-II, G-III and G-IV. All fetuses in G-IV combination had malforamtions. Main malformation in G-IV combi. nation were microstomia, disposition of ear, open eyelids brachygnathia and cleft plate. Accordingly it might be demonstrated that the teratogenicity of hypervitaminosis A was pontentiated by concurrent ethanol in rats.

Quinone계 화합물의 발암성 조기검색법에 관한 연구

조대현,홍진태,박정식,홍연탁,진강,정명희,이병무,Cho, Dae-Hyun,Hong, Jin-Tae,Park, Jeong-Sik,Hong, Youn-Tack,Chin, Kang,Jung, Myung-Hee,Lee, Byung-Mu 한국독성학회 1992 Toxicological Research Vol.8 No.2

To investigate a short term screening method for carcinogenic quinone compounds, 8-hydroxydeoxyguanosine (8-OHdG), an oxidative DNA damage, was determined in the kidney and liver DNA isolated from Sprague-Dawley rats after i.p.injection of 7 mg/kg adriamycin (AM), 7mg/kg tetrahydropyranyladriamycin (THP), and 10mg/kg daunomycin (DM) by HPLC-electrochemical detector system. 8-OHdG was also determined from rat hepatocvtes and calf thymus DNA exposed to AM, DM and THP. When rats were treated with DM and THP, 8-OHdG was significantly increased in the kidney compared to control group, and remained at high level (7.9~9.0, 8-OHdG/dG${\times}10^4$)at the end of experiments (48hr after treatment). 8-OHdG level in cultured hepatocyte exposed to AM, DM and THP was 1.5~2 fold higher than control at all time points. (1,2,3,4hr after treatment). From calf thymus DNA exposed to AM, DM and THP, 8-OHdG was 2.5 fold higher than of control. These results suggest that quantitation of 8-OHdG may provide a useful marker for identifying target organ in oxidative chemical carcinogenesis and for short term screening of free radical generating carcinogens.

마약류 및 산업환경화학물질에 의한 GFAP의 신경독성표지물질화에 관한 유용성

조대현,정용,김준규,이봉훈,황세진,이원용,김정구,조태순,김진석,문화회,Cho, Dae-Hyun,Jeong, Yong,Kim, Jun-Gyou,Lee, Bong-Hun,Hwang, Se-Jin,Lee, Won-Yong,Kim, Jeong-Goo,Cho, Tai-Soon,Kim, Jin-Suk,Moon, Hwa-Hwey 한국독성학회 1995 Toxicological Research Vol.11 No.2

Diverse neurotoxic insults result in proliferation and hypertrophy of astrocytes, a subtype of glia in central nervous system. The hallmark of this response, often terms "reactive gliosis", is the enhanced expression of the major intermediate filament protein of castrocytes, glial fibrillary acidic protein (GFAP). These changes in the astrocytes suggest that GFAP may be a useful biochemical indicator of neurotoxicity. To investigate this possibility, we administered intra-peritoneally prototype nerotoxicants, metharnphetamine (MAP, 5 mg/kg), cocaine (30 mg/kg), N-buthyl benzenesulfonamide (NBBS, 300 mg/kg) and trimethytin (TMT, 8 mg/kg) to Wistar Rats and then assessed the effects of these agents on content of GFAP, which were determined by Sandwish ELISA and evaluated with neurotoxic symptoms, and quantitative changes of imrnunoreactivity of GFAP by light microscopic image analysis in specific regions. We found that assay of GFAP revealed time- and region-dependant patterns of neurotoxicity. The GFAP immunoreactivity of rat brain was increased in substantia nigra and hippocampus by MAP, NBBS and TMT; in roedial septal nucleus and nucleus accurnbens, it was also increased by RrBBS. Sandwich ELISA showed that GFAP levels of cerebrum in all groups on days 3 and 7 and that of brainstem(including cerebellum) in MAP, NBBS groups on day 1 and 3 were increased. A review of the background, design and results of these experiments are presented in this paper. Our findings indicate that GFAP is a sensitive and specific biomarker of neurotoxicity.otoxicity.

차량의 연료 소비 감소를 위한 병목 도로에서 도로 교통 정보 기반 Green Drive 제어에 관한 연구

조대현,이충훈,임명섭,Cho, Dae-Hyun,Lee, Chung-Hoon,Lim, Myung-Seob 한국융합신호처리학회 2012 융합신호처리학회 논문지 (JISPS) Vol.13 No.3

본 논문에서는 차량의 흐름을 지체시키는 병목 지점에서 차량의 연료소비 감소를 위한 WAVE 방식 기반 V2V 및 V2I 의 정보 통신 기술을 이용하여 차량의 상대적인 속도를 제어하는 방법을 제안하였다. 연비 효율 비교 분석을 위한 모델을 제시하여 주기적으로 차량의 정차를 요하는 교통 신호등 방식보다 제안하는 방식이 병목 지점에서 연비 효율이 높아짐을 보였다. 또한 이 방식에서 병목지점에 진입하는 차량 간 속도 제어를 위한 감 가속도 유도 알고리즘을 제시하였다. In this paper, the method of controlling velocity between joining vehicles at the point of bottleneck using information and communications technology of WAVE method based V2V and V2I are proposed for the driving with high fuel efficiency. Using the derived fuel-efficiency comparative analysis model, it was shown that the proposed method's fuel efficiency is better than traffic light method demanding periodically vehicle's stop. Also, this method provides the derivation algorithm for deceleration and acceleration for controlling velocity between vehicles approaching bottleneck area.

신경세포 배양법을 이용한 methamphetamine과 cadmium의 신경독성 평가

조대현,김준규,정용,이봉훈,김은엽,김정구,조태순,김진석,문화회,Cho, Dae-Hyun,Kim, Jun-Gyon,Jeong, Yong,Lee, Bong-Hun,Kim, Eun-Youb,Kim, Jeong-Goo,Cho, Tai-Soon,Kim, Jin-Suk,Moon, Hwa-Hwey 한국독성학회 1996 Toxicological Research Vol.12 No.1

Primary culture of cerebellar neuronal cells derived from 8-day old Long-Evans rats was used. Pure granule cells, astrocytes or mixed cells culture systems were prepared. These cells were differentiated and developed synaptic connections. And the astrocytes were identified by immunostaining with glial fibrillary acidic protein (GFAP). Methamphetamine (MAP), which acts on dopaminergic system and cadmium (Cd), a toxic heavy metal, were applied and biochemical assays and electrophysiological studies were performed. $LC_50$ values estimated by MTT assay of MAP and Cd were 3 mM and 2$\mu M$ respectively. Cells were treated with 1 mM or 2 mM MAP and 1$\mu M$ $CdCl_2$ for 48 hour, and the incubation media were analyzed for the content of released LDH. MAP (2 mM) and Cd significantly increased the LDH release. Cell viability was decreased in both groups and some cytopathological changes like cell swelling or vacuolization were seen. The cerebellar granule cells were used for measuring membrane currents using whole-cell clamp technique. Sodium and potassium currents were not affected by MAP neither Cd, but calcium current was significantly reduced by Cd but not affected by MAP. Therefore, in vitro neurotoxicity test system using neuronaI cells and astrocytes cultures were established and can be used in screening of potential neurotoxic chemicals.

음악 중심 융합교육의 구현을 위한 제언

조대현 ( Dae Hyun Cho ) 민족음악학회 2014 음악과 민족 Vol.48 No.-

This study aims at examining the possibility and the requirements of convergence education in music education through a theoretical contemplation on convergence and convergence education, and at offering a method for realizing music-centered convergence education focused around various examples. For this purpose, the researcher has firstly examined the meaning and the role of convergence and convergence education from various theoretical viewpoints, and as a result could offer major principles and examples for application in the classrooms as well as improvement points and limitations. At this point, it has been discovered that the degree of understanding of “the difference between integration and convergence” and “agents of convergence” on the part of advance organizers who design and arrange the environment for convergence is a very important element.

가변 이득을 가지는 슬라이딩 모드 제어를 이용한 수차 발전 시스템의 성능 향상

조대현(Dae-Hyun Cho),박영수(Yeongsu Bak),이교범(Kyo-Beum Lee) 대한전기학회 2019 대한전기학회 학술대회 논문집 Vol.2019 No.7

동적계획기법과 다중회귀분석및 통합 모의를 이용한 저수지 최적 운영률 결정

조대현 ( Cho Dae-hyun ) 한국환경기술학회 2001 한국환경기술학회지 Vol.2 No.2

동적 계획 기법과 다중회귀 분석 및 통합모의를 이용하여 저수지 최적 운영률을 결정방법을 연구하였다. 대상 저수지로서 대청댐을 선정하여 1981년부터 1999년까지 운영실적에 적용하였다. 본 연구에서 채택한 알고리즘은 다중 저수지 체계에 대하여 개발된 것이나, 본 연구에서는 단일 저수지 체계에 적용하였다. 본 연구의 결과는 저수지 관리와 운영에서 손실함수와 정책대안의 관계를 설정하는 데 활용될 수 있을 것이다. This study is purposed to develope optimal reservoir operation rules by integrating dynamic programming, regression analysis, and simulation which are deterministic approach method. The Daechung dam is employed and the operation period is from 1981 to 1999 years. Employed algorithm is adjusted to multidimensional reservoir systems,but is applied single reservoir system in this study. The results of this study might be used to formulate the relation between loss function and policies for the reservoir management and operation.

VBR 비디오 서버를 위한 확률 모델 기반의 최적 자원 구성

조대현(Dae Hyun Cho),손진현(Jin Hyun Son),김명호(Myoung Ho Kim),이윤준(Yoon Joon Lee) 한국정보과학회 2001 정보과학회논문지 : 데이타베이스 Vol.28 No.3

최근 대부분의 비디오 서버에서는 VBR 비디오를 사용하고 있고, VBR 비디오의 디스플레이 비율은 기존의 CBR 비디오의 디스플레이 비율과는 달리 시간에 따라 변하기 때문에, 기존의 CBR 기반의 자원 구성 방법을 VBR 비디오 서버의 자원 구성에 사용하기에는 적합하지 못하다. 본 논문에서는 확률모델 기반의 효율적인 VBR 비디오 서버를 위한 자원 구성 방법을 제안한다. 이 방법은 비디오 서버의 처리량은 그대로 유지하면서 하드웨어 비용을 최소화시킬 수 있는 디스크와 메모리 양, 그리고 비디오 서버의 디스크 접근 주기 등과 같은 변수값들을 결정한다. 추가적으로 실험을 통하여 제안된 자원 구성 방법의 유용함을 확인한다. Most of currently used videos have variable bit rate(VBR) characteristics. Since the display rate of VBR videos compared to CBR videos vary with time, it is not proper to configure resources of the VBR video server using the method proposed for the CBR video server. In this paper we propose an optimal resource configuration method for the VBR video server which is based on the probability model. The proposed method decides the amount of disk and memory, and the disk access cycle of the video server with the lowest hardware cost, while preserving the throughput of the video server. In addition, we show the usefulness of the method through the various experiments.

학교음악교육에서의 긍정적 현상학적 장 형성과 이를 위한 전제조건

조대현 ( Dae Hyun Cho ) 민족음악학회 2010 음악과 민족 Vol.39 No.-