http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 조기승 ( Tae Kyung Park (검색결과 5 건)
- 무료
- 기관 내 무료
- 유료
-
인삼 사포닌의 생화학적 연구 (XVIII) : 쥐 간 균질물에 의한 인 지질 생합성에 미치는 인삼 사포닌의 영향
박태경,주충노,조기승,Park, Tae-Kyung,Joo, Chung-No,Cho, Key-Seung 생화학분자생물학회 1981 한국생화학회지 Vol.14 No.4
쥐의 간 균질물을 효소원으로 사용하고 $(U-^{14}C)$-glycerol, $(U-^{14}C)$-sn-glycerol-3-phosphate 또는 $(1-^{14}C)$-palmityl CoA를 tracer로 사용하여, 인 지질 생합성에 미치는 인삼 saponin의 영향을 in vitro에서 관찰하여 다음과 같은 결과를 얻었다. 1. $(U-^{14}C)$-Glycerol을 사용한 실험에서 phosphatidyl ethanolamine 생합성은 인삼 saponin의 농도가 $10^{-4}{\sim}10^{-3}%$일 때 대조군 보다 10배 이상 증가되었다. 2. $(U-^{14}C)$-sn-Glycerol-3-phosphate를 사용한 실험에서 인삼 saponin의 농도가 $10^{-4}{\sim}10^{-3}%$ 일 때 대조군에 비해서 전 지질의 생합성이 촉진되었고, 인삼 saponin의 농도가 $10^{-4}%$ 일 때 phosphatidyl ethanolamine, phosphatidyl oholine 및 neutral lipid의 생합성은 대조군 보다 증가되었다. 3. $(1-^{14}C)$-Palmityl CoA를 사용한 실험에서 인삼 saponin의 농도가 $10^{-4}{\sim}10^{-3}%$ 일때 phosphatidyl ethanolamine 및 phosphatidyl choline의 생합성이 촉진되었다. It has been attempted in this study to observe the effect of ginseng saponin on phospholipid biosynthesis by rat liver homogenate using $(U-^{14}C)$-glycerol, $(U-^{14}C)$-sn-glycerol-3-phosphate, and $(1-^{14}C)$palmityl CoA as tracer in vitro and the following results were obtained. 1. Biosynthesis of phosphatidyl etanolamine using $(U-^{14}C)$-glycerol as tracer was increased as much as 10 times when the concentration of ginseng saponin in the reaction mixture was $10^{-4}\sim10^{-3}%$. 2. In case of experiment using $(U-^{14}C)$-sn-glycerol-3-phosphate as tracer, biosoynthesis of total lipids was also stimulated at concentration of $10^{-4}\sim10^{-3}%$ of ginseng saponin. Furthermore, biosynthesis of phosphatidyl ethanolamine and phophatidyl choline was increased in the reaction mixture containing $10^{-4}%$ ginseng saponin. 3. Using liver homogenate and $(1-^{14}C)$-palmityl CoA only, it was observed that the ginseng saponin $(10^{-4}\sim10^{-3}%)$ stimulated the biosynthesis of phosphatidyl choline and phosphatidyl ethanolamine. From the above experimental results, it seemed that adequate amounts of ginseng saponin might stimulate the biosynthesis of phospholipids significantly.
-
인삼 사포닌의 생화학적 연구 ( ⅩⅧ ) 쥐 간 균질물에 의한 인 지질 생합성에 미치는 인삼 사포닌의 영향
박태경,주충노,조기승 ( Tae Kyung Park,Chung No Joo,Key Seung Cho ) 생화학분자생물학회 1981 BMB Reports Vol.14 No.4
It has been attempted in this study to observe the effect of ginseng saponin on phospholipid biosynthesis by rat liver homogenate using (U-^(14)C)-glycerol, (U-^(14)C) -snglycerol-3-phosphate, and (1-^(14)C)-palmityl CoA as tracer in vitro and the following results were obtained. 1. Biosynthesis of phosphatidyl etanolamine using (U-^(14)C)-glycerol as tracer was increased as much as 10 times when the concentration of ginseng saponin in the reaction mixture was 10^(-4)∼10^(-3). 2. In case of experiment using (U-^(14)C)-sn-glycerol-3-phoshate as tracer, biosoynthesis of total lipids was also stimulated at concentration of 10^(-4)∼10^(-3)% of ginseng saponin. Furthermore, biosynthesis of phosphatidyl ethanolamine and phophatidyl choline was increased in the reaction mixture containing 10^(-4)% ginseng saponin. 3. Using liver homogenate and (1-^(41)C)-palmityl CoA only, it was observed that the ginseng saponin (10^(-4)∼10^(-3)%) stimulated the biosynthesis of phosphatidyl choline and phosphatidyl ethanolamine. From the above experimental results, it seemed that adequate amounts of ginseng saponin might stimulate the biosynthesis of phospholipids significantly.
-
-
김기태,홍사원,원호식,김효준,박경배,조기승,Kim, Ki-Tae,Hong, Sa-Weon,Won, Ho-Shik,Kim, Hyo-Joon,Park, Kyung-Bae,Cho, Key-Seung 한국미생물학회 1998 미생물학회지 Vol.34 No.1
지네 Scolopendra subspinipes multilans L. Koch로부터 정제한 Centipedin의 gram-negative, gram-positive bacteria 및 fungi에 대한 광범위 항균 spectrum을 조사하였으며 액체배지를 사용하여 각 균주에 대한 minimal inhibitory concentrations(MICs)값을 측정하였다. 인체의 기관중 주로 폐에 감염되는 gram-negative bacteria인 Klebsiella pneumoniae ATCC 8308 균주에 대한 MIC값이 $2{\mu}g/ml$로서 가장 강한 항균력을 나타내었고 화농균인 Proteus vulgaris NRRL B-123 균주에 대해서도 MIC값이 $16{\mu}g/ml$로 비교적 높은 항균력을 나타내었으며 항균제로서의 작용 mechanism을 시험한 결과 prokaryotic RNA transcription을 억제함을 알 수 있었다. Human blood leukemia(HL-60)와 mouse B lymphocyte myeloma cell 등의 동물세포에 대한 Centipedin의 cytotoxicity는 없는 것으로 확인되었다. A broad spectrum of antibiotic action was studied with Centipedin purified from centipede Scolopendra subspinipes multilans L. Koch aganist gram-positive, gram-negative bacteria and fungi. The minimal inhibitory concentrations(MICs) were determined in liquid medium. The significant antibiotic activity was obtained aganist gram-negative Klebsiella pneumoniae ATCC 8308 responsible for causing infection at lung and intestine. The MIC value against Klebsiella pneumoniae ATCC 8308 was $2{\mu}g/ml$, and this Centipedin was active against Proteus vulgaris NRRL B-123. In addition, it has been shown that Centipedin blocks procaryotic RNA transcription and a little of DNA replication system in vitro. Centipedin did not exhibit any significant cytotoxicity against animal cells such as human blood leukemia (HL-60) and mouse B lymphocyte myeloma cell.
-
Centipede Scolopendra subspinipes multilans L. Koch로부터 항균력이 있는 Centipedin의 추출, 정제 및 특성연구
김기태,최진성,민천홍,정일엽,박경배,조기승 한양대학교 이학기술연구소 1999 이학기술연구지 Vol.1 No.-
항균력이 있는 화합물인 Centipeding을 지네 Scolopendra subspinipes multilans L. Koch로부터 지용성 유기용매로 추출하였고, silicic acid column과 high S cation exchange chromatography 및 reverse-phase HPLC 등을 사용하여 고순도로 정제하였다. Gram-positive bacteria인 Staphylococcus aureus ATCC 6538P 및 gram-negative인 Klebsiella pneumoniae ATCC 8308 균주에 대하여 강한 항균력을 나타내었다. Dragendorff 시약에 의한 발색반응을 시행한 결과 Centipedin의 aromatic ring에는 nitrogen이 없음을 확이하였고 IR, Mass, NMR 분석 등에 의하여 Centipedin의 분자량은 162.1, 화학적 구조는 hydroxyl기와 carbonyl기를 함유하는 -hydroxyisocumarin으로 확인되었다. UV-spectrum 검색결과에 의하면 최대 흡수파장이 243 ㎚임을 확인하였고, melting point는 122℃이었으며, 실온에서 3개월간 방치된 Centipedin이 여전히 항균활성 및 화학적 구조를 유지하여 물리화학적 및 생물학적으로 안정한 화합물임으 알 수 있었다. The compound which has antibiotic activity was extracted and purified from centipede Scolopendra subspinipes multilans L. Koch by diethyl ether, silicic acid column and high S cation exchange chromatography followed by reverse-phase HPLC. This compoung named "Centipedin" showed antibiotic activity aganist microorganisms such as Gram^(+) -bacteria Staphylococcus aureus ATCC 6538P and Gram^(-) -bacteria Klebsiella pneumoniae ATCC 8308. Dragendorff test showed that there is no aromatic nitrogen within the Centipedim. The molecular weight of the Centipedin was 162.1 daltons as determined by mass spectrometry. The instrumental analysis of IR and NMR techniques revealed the molecular structure of Centipedim as 8-hydroxyisocoumarin. The UV-spectrum of Centipedin showed maximum absorption at 243 ㎚, and melting point was 122℃. Centipedin was stable at room temperature for three months in appropriate buffers.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
