항산화제에 의한 유전자발현 조절에 관한 연구

정성오(Sung Oh Chung) 산업기술교육훈련학회 2014 산업기술연구논문지 (JITR) Vol.19 No.3

Ascorbic acid is a natural confound that is commonly biosynthesized in many animals except primates, guinea pigs, and some birds and possess an unusually high antioxidant activity. The aim of the present study was to investigate whether ascorbic acid could have anti-inflammtory effect in cells. In order to know whether the ascorbic acid would have the suppressive effect on the inflammatory mediators or cytokines gene expression. The levels of interleukin—6QL-6), tumor necrosis factor(TNF-a), cyclooxygenase-2(COX-2), RANTES, and inducible nitric oxide synthase(iNOS) expression were examined in lipopolysaccaride(LPS) - stimulated RAW 264.7 cells in the presence or absence of ascorbic acid treatment. LPS increased these genes expression in RAW264.7 cells. It was found that this increase was suppressed by ascorbic acid pre-treatment in LPS-stimulated RAW 264.7 cells. Ascorbic acid also suppressed reactive oxygen species(ROS) or reactive nitrogen species(RNS) production in LPS-stimulated RAW 264.7 cells. Therefore, these observations suggest that ascorbic acid can function as a regulator on the gene expression level.

한국 성인의 지역적 요인에 따른 치주 상태 비교

정선영 ( Sun Young Chung ),정성화 ( Seong Hwa Jeong ),빈성오 ( Sung Oh Bin ),최연희 ( Youn Hee Choi ),송근배 ( Keun Bae Song ) 대한예방치과·구강보건학회 2010 大韓口腔保健學會誌 Vol.34 No.4

연구목적. 치주질환은 성인의 구강 상태에 영향을 미치는 주요한 요인으로, 최근 사회경제적 요인이나 지역적 차이에 따른 구강상태에 대한 관심이 증가하고 있다. 본 연구는 2000년 전국민 구강건강실태조사 자료를 활용하여 한국 성인을 대상으로 거주지역 요인과 치주 상태와의 관련성을 알아보고자 하였다. 연구방법. 국민구강건강실태조사2000 데이터베이스에서 18세에서 90세 6,525명의 자료를 선별하여 지역(대도시, 중소도시, 농촌)에 따라 구분하였다. 구강검사는 세계보건기구(WHO) 기준에 따라 실시하였고, 치주 상태는 지역사회 치주지수(CPI)에 근거하여 결정하였다. 다중회귀분석을 통해 지역적 요인과 치주 상태와의 관련성을 분석하였으며, 통계 분석은 SAS 9.1.3을 이용하였다. 연구결과. 지역에 따른 치주염 유병률은 대도시, 중소도시, 농촌에서 각각 21.4%, 30.0%, 32.6%로 나타났다(p<0.01). 지역적 요인과 치주 상태와의 관련성을 살펴본 결과 다른 지역과 비교하여 농촌에서 치주염 유병률이 높은 것으로 나타났으나 (OR=1.776, 95% CI [1.562, 2.020]), 사회경제적 요인, 주관적 건강 인식, 건강 관련 행동, 전신질환 유무의 혼란 변수를 보정한 결과, 중소도시에서 가장 높은 것으로 나타났다(OR=1.218, 95% CI [1.026, 1.446]). 결론. 우리나라 성인의 치주 상태는 거주 지역 요인과 관련이 있는 것으로 나타났으며, 본 연구 결과는 구강건강의 지역적 불평등 해소를 위한 대책 마련에 기여할 수 있을 것으로 기대한다.

유해활성산소 및 유해산화질소가 유발하는 핵과 소포체의 변화 분석

김연향(Yeon Hyang Kim),정은영(Eun Young Jung),정지윤(Ji Yun Jung),정성오(Sung Oh Chung) 산업기술교육훈련학회 2014 산업기술연구논문지 (JITR) Vol.19 No.3

To investigate cross-talk between endoplasmic reticulum(ER) stress and oxidative stress in astroglioma, the cells were treated with hydrogen peroxide, DETA-NONOate and thapsigargin to raise intracellular levels of oxidative stress and ER stress, respectively. Cell viability was decreased in a dose-dependent manner in hydrogen peroxide- and DETA-NONOate - treated cells. Hydrogen peroxide, DETA-NONOate and thapsigargin also induced DNA fragmentation in these cells as indicated by terminal deoxynucleotidyl transferase-med iated dUTP nick end labeling. Hydrogen peroxide, DETA-NONOate and thapsigargin induced intracellular Ca2+ increase and perturbed Ca2+ homeostasis in endoplasmic reticulum(ER). Likewise, hydrogen peroxide, DETA-NONOate and thapsigargin also increased the expressions of ER stress- related molecules, IREl-a. The increase of IRE1 -a expression is coincided with those of p-JNK up on the treatment with hydrogen peroxide, DETA-NONOate, and thapsigargin in cells. These finding indicate that the increase of expression of IREl-a activating JNK pathway is induced during hydrogen peroxide, DETA-NONOate and thapsigargin - mediated cell death. Therefore, we conclude that oxidative stress including reactive oxygen species and nitric oxide cause nucleus and endoplasmic reticulum(ER) dysfunction and ER-related signals to occur in human astroglioma cells.

담낭의 육종양 암종 1예

위준한(Jun Hwan Wi),안명수(Myoung Soo Ahn),정성오(Sung Oh Chung),정안철(An Chul Jeong),이승복(Seung Bok Lee),서창인(Chang In Suh),권소영(So Young Kwon),장희진(Hee Jin Chang) 대한내과학회 1999 대한내과학회지 Vol.57 No.6

N/A Sarcomatoid carcinomas are rare neoplasms that have been described using various terms, pseudosarcoma, carcinosarcoma or spindle cell carcinoma. Sarcomatoid carcinomas are epithelial tumors with sarcomatoid components and characterized by the presence of cytokeratin in pleomorphic spindle cells. These tumors of gallbladder rarely have been reported predominantly in older women with associated gallstones and their prognosis was very poor. However, the clinical and histological data are very limited. We report a case of sarcomatoid carcinoma in gallbladder confirmed by light microscopy and immunohistochemistry.(Korean J Med 57:1048-1052, 1999)

당뇨병 교육 방법이 당뇨병 환자의 식사요법 수행과 당화혈색소에 미치는 영향

김을상,문현경,이영남,이태훈,김성곤,고재민,손진희,유형준,송오금,남흥우,정성오,위준환,염주협,조대경 대한당뇨병학회 2000 Diabetes and Metabolism Journal Vol.24 No.5

Background: Diet control plays an important role in diabetic management, but it is often hard for diabetic patients to follow the dietary control program. Poor dietary compliance leads to metabolic derangements in patients with diabetes and it may derive mainly from defects in dietary education program rather than from patients themselves. Therefore, we performed a randomized prospective study to compare the effects of three different teaching methods for diet control. Methods: Forty eight diabetic patients with poor glycemic control (mean HbA1c 11.4±1.5%) were enrolled during hospitalization and allocated at random to three different teaching methods i.e. Conventional diet sheet instruction (Group 1), Food recording on every meal (Group 2), and Meal time demonstration (Group 3). For evaluation, knowledge about DM diet and barriers to diet control were assessed by a questionnaire. Consistency in carbohydrate intake (Coefficient of variation) and serial HbA1C measurements were used for the estimation of dietary compliance and glycemic control respectively. Results: During five months' follow-up period, there was no remarkable improvement in knowledge about diabetic diet control, dietary compliance and glycemic control in Group 1 patients. But both dietary compliance and glycemic control improved in Group 2 and 3 patients during follow-up period. In Group 2 CV (Coefficient of Variation) fell from 36.4±15.2% to 27.7±17.3% and in Group 3 from 32.1±9.6% to 23.2±10.5% (p$lt;0.05). In Group 2 HbA_(1c) fell from 12±2.2% to 8.3±2.0% and in Group 3 from 11.5±2.0% to 7.5±1.9%(p$lt;0.01). The change of HbA1c level showed an appreciable correlation with dietary compliance (r= 0.75). Among the perceived barriers to dietary practice in patients of Group 2 and Group 3, extrinsic factors related to knowledge lowered during the intervention (p$lt;0.05). Even though Group 3 patients had good dietary compliance, they still felt that intrinsic factors related to motive and attitude were the major barriers at the end of the study (p$lt;0.05). Conclusion: We found that meal time demonstration teaching method may improve dietary compliance and glycemic control compared with the conventional diet sheet instruction method.

혈액 투석기의 재사용시 투석막에 따른 혈액 요소 질소와 β2-Micr0globulin 제거율의 변화

고재민,손진희,정성오,이태훈,조대경,송성욱,정예경,전용덕 대한신장학회 2000 대한신장학회잡지 Vol.19 No.6

노인 심방세동 환자의 원인기저 질환 분석

조대경,위준환,염주협,고재민,이태호,정성오,김태훈,정봉기,남홍우,이홍순,유수웅,문언수 대한노인병학회 2000 Annals of geriatric medicine and research Vol.4 No.3

유방암과 동반된 피부근염에서 발생한 횡문근융해증 및 급성 신부전 1예

김용훈,손진희,염주협,정예경,안병진,정성오,전용덕 대한신장학회 1999 Kidney Research and Clinical Practice Vol.18 No.2

Dermatomyositis is a clinical syndrome of unknown etiology characterized by a nonpurulent inflammatory myopathy involving striated skeletal muscle associated with rheumatoid arthritis, or systemic lupus erythematosus, sometimes underlying malignancy. A few cases of acute renal failure due to rhabdomyolysis in dermatomyositis has been reported. But, no case combined with breast cancer have been reported. We experienced a case of acute renal failure due to rhabdomyolysis associated with dermatomyositis. The patient was admitted to the our hospital due to breast cancer. After breast cancer operation, she complained of progressive muscle weakness. The heliotrope rash involved the eyelid, bridge of nose and forehead. And eczematoid dermatitis involved the lower abdomen and both forearm and hands. The laboratory findings revealed LDH 2,944IU/L, CPK 2,244IU/L and SGOT 214IU/L. Serum myoglobin and aldolase were increased, and antinuclear antibody was 1:40(positive, homogenous pattern). Electromyogram revealed myopathic findings compatible with dermatomyositis. Then, the patient developed acute renal failure requring hemodialysis. After acute hemodialysis, acute renal failure resolved. But, the patient died on the 113th hospital day due to sepsis. Here, we report a case of acute renal failure due to nontraumatic rhabdomyolysis associated with dermatomyositis in breast cancer.