설치류에서 정소조직의 체외배양을 통한 정자형성과정에 관한 연구

정미선 ( Mi Seon Jung ),김방진 ( Bang Jin Kim ),이용안 ( Yong An Lee ),김기중 ( Ki Jung Kim ),김용희 ( Yong Hee Kim ),강현구 ( Hyun Gu Kang ),정상은 ( Sang Eun Jung ),김병각 ( Byung Gak Kim ),류범용 ( Buom Yong Ryu ) 한국조직공학과 재생의학회 2015 조직공학과 재생의학 Vol.12 No.1s

Spermatogenesis is a complex developmental process of continuous cell division involving a phase of germ cell expansion, meiotic division and cytodifferentiation that ultimately generates mature gametes. The object of this study was the development of optimal culture system in order to induce spermatogenesis in vitro and for its establishment. We collected immature testicular tissues from different developmental-stage of mice and rats which were subsequently cultured on the agarose gel with serum-free culture media. After appropriate culture period, we counted multiple layered seminiferous tubules in the cultured testis tissue to find out whether any differentiation occured following spermatogenesis. Between our experimental mouse strains ICR and C57, as a result, we observed significant increased spermatogenic tubules in case of C57 mouse strain in neonatal stage. In our optimized culture condition, we detected the lectin PNA positive spermatid resulted from spermatogenesis in cultured testis tissue after 6 weeks of incubation. We also injected the lentiviral transduced rat spermatogonial stem cells (SSCs) into recipient testis to generate the transgenic gametes. The testicular fragments were then cultured based on our optimized culture condition. After culturing 8 weeks, we observed expression of GFP in differentiated germ cells including post meiotic germ cells, which suggested the induction of donor-derived spermatogenesis. Also, in order to optimize the in vitro culture system for rat testis, we have tested the additional effect of hormones and growth factors, but could not observe the significant increased effect of hormone and growth factors treated groups than the control. In conclusion, our in vitro tissue culture methods could be applicable for production of in vitro transgenic gametes in various kinds of mammalian species including domestic animal.

고양이를 돌보는 경험에 관한 미술치료사의 자전적 내러티브 탐구

정미선(Jung Mi-sun),최선남(Choi Sun-nam) 한국미술치료학회 2020 美術治療硏究 Vol.27 No.4

본 연구는 나와 살고 있는 반려묘뿐만 아니라 길고양이를 돌보는 경험이 나의 삶과 나아가 미술치료사로서 의 삶에 어떠한 의미가 있는지 알고자 하는데 목적이 있다. 고양이를 돌보는 경험에 대한 깊은 이해와 의미에 집중하기 위해 자전적 내러티브 탐구 방법을 사용하였으며, 고양이에 대한 탐구자의 그림, 글쓰기, 편지, 회상, 대화 등의 표현을 통해 탐구가 진행되었다. 자료 수집은 2014년 5월부터 2019년 11월까지 이루어졌다. 이 과정에서 생성된 경험의 이야기는 ‘내 발길을 되돌아가게 만드는 나약한 존재, 길고양이 나비’, ‘상실의 아픔을 준 나의 고양이, 사랑이’, ‘사랑이의 상실로 만난 새로운 고양이, 별이’, ‘마음의 줄다리기를 하게 하는 길고양이, 요미’의 4가지로 생성되었다. 탐구자의 경험은 ‘안쓰러움, 나약함’, ‘돌봄과 책임의 경계’, ‘본능과 이성 사이(자율과 억압)’, ‘공감은 언어를 넘어서서 이루어진다’의 의미를 생성하였다. 본 탐구는 나를 자유케 하였고, 이해의 선물은 대상을 초월해서 야기되고, 동물학대에 대한 경각심을 전하는 계기가 되었다. The purpose of this study was not only to examine my cat but also to find the meaning stray cats have in my life and in the lives of art therapists. I used a process called Autobiographical Narrative Inquiry Methodology to focus on understanding my experiences in raising a cat. The study was carried out by looking at my paintings, letters, reminiscences, writings and conversations about cats. Data collection took place from May 2014 to November 2019. I put together four stories using this process: a stray cat, named Nabi who kept making me look back, Sarang-ee who gave me the pain of losing a pet, a new cat -Byul-ee-, who came to my life after I lost my Sarang-ee, and finally a stray cat named Yomi who made me play hard to get. Reflecting on these experiences brought up several ideas about cats: “pitiful weakness,” “boundaries between care and responsibility,” “gap between instincts and rationality,” “empathy comes from something beyond language.” This study liberated me, and gave me the gift of understanding and delivered insights that transcended my goal of studying animal abuse.

추출방법에 따른 산초 종자 정유성분의 이화학적 특성

정미선 ( Mi Seun Jung ),신연미 ( Yeon Mi Shin ),김명규 ( Myeong Kyu Kim ),김철호 ( Chul Ho Kim ),최진상 ( Jine Shang Choi ) 한국식품저장유통학회(구 한국농산물저장유통학회) 2013 한국식품저장유통학회지 Vol.20 No.6

황색착색렌즈를 이용한 착색 청색광차단렌즈의 분석과 평가 방법에 관한 고찰

정미선(Mi-Sun Jung),최은정(Eun Jung Choi) 한국안광학회 2018 한국안광학회지 Vol.23 No.1

Purpose: Using yellow-tinted lenses prepared for this study and blue light blocking tinted lenses sold on the domestic market, we examined analysis and evaluation methods for the development and performance improvement of tinted blue light blocking lenses. Methods: The yellow-tinted lenses were prepared with CR-39 lenses and yellow powder dye, the spectral transmittances of lenses were measured using a UV-Vis spectrophotometer, and the fitting curves related to the tinted time, luminous transmittance and blue light blocking ratio were derived through regression analysis. Results: The relationships between the tinted time and luminous transmittance, the tinted time and blue light blocking ratio, and the blue light blocking ratio and luminous transmittance were well fitted with the double exponential decay curve. Using the fitting curves calculated the tinted time, luminous transmittance, and blue light blocking ratio required to produce the blue light blocking lenses intended to be targeted. In addition, using the R<SUB>BL</SUB>-τ chart quickly and easily compared the blue light blocking ratio and luminous transmittance determining the performance of the Blue light blocking lens. Conclusions: We suggest that the fitting curves and R<SUB>BL</SUB>-τ chart related to the tinted time, luminous transmittance, and blue light blocking ratio can be usefully utilized in estimating the performance of the blue light blocking lens and expect them to be widely available.

상담 전공 특수대학원생의 상담 누적시간, 수퍼비전 누적시간이 진로걱정에 미치는 영향

정미선(Jung, Misun) 한국웰니스학회 2018 한국웰니스학회지 Vol.13 No.3

본 연구는 상담을 전공으로 하는 특수대학원에 재학 중인 대학원생들을 대상으로 상담 누적시간과 수퍼비전 누적시간 각각이 가지는 진로걱정과의 관계에 있어서 상담자활동 자기효능감이 조절효과를 보이는지를 알아보았다. 이를 위해 상담 전공 특수대학원 재학생 232명을 대상으로 설문조사를 실시하였으며 상관분석과 위계적 회귀분석을 하였다. 그 결과는 다음과 같다. 첫째, 상담 누적시간은 상담자활동 자기효능감과의 상호작용을 통해 진로걱정과의 관계에서 조절효과가 나타났다. 둘째, 수퍼비전 누적시간의 경우에는 상담자활동 자기효능감의 하위요인 중 상담난제 자기효능감과만 상호작용을 통해 진로걱정과의 관계에서 조절효과를 가지는 것으로 나타났다. 이는 상담대학원생들의 진로걱정을 낮추는데 상담자활동 자기효능감이 중요한 변인으로 영향을 미칠 수 있음을 보여주는 결과이다. 마지막으로 이러한 연구 결과를 토대로 시사점과 제한점, 후속연구를 위한 제언을 제시하였다. The purpose of this study was to investigate effects of cumulative counseling hours and cumulative supervision hours on career worries, and identify the role of counselor activity self-efficacy as a moderating variable in the relationship between cumulative counseling hours or cumulative supervision hours and career worries among graduate students with counseling major. A total of 232 counseling graduate students in specialized graduate school of counseling completed the research questionnaire. The results were as follows. First, counselor activity self-efficacy played a moderator in the relationship between the cumulative counseling hours and career worries. Second, the counseling challenges self-efficacy only among counselor activity self-efficacy variables moderated in the relationship between the cumulative supervision hours and career worries. This result indicated that counselor activity self-efficacy could be a preventive factor for career worries. Finally, the limitations and the implications of the study were discussed.

라이프스타일에 따른 자동차 인테리어 발전 방향에 관한 연구

정미선(Jung Mi-Sun),류승훈(Ryu Seung-Hoon),윤상훈(Yoon Sang-Hun),김철수(Kim Chul-Soo),박영목(Park Young-mok),인석일(In Suk-Il),박종서(Park Jong-Suh) 한국디자인학회 2003 한국디자인학회 학술발표대회 논문집 Vol.- No.-

녹용 유래 중간엽 줄기세포의 체외 배양 효율 증진

김기중 ( Ki Jung Kim ),유형덕 ( Hyung Duk Yoo ),김용희 ( Yong Hee Kim ),이용안 ( Yong An Lee ),김방진 ( Bang Jin Kim ),정미선 ( Mi Seon Jung ),강현구 ( Hyun Gu Kang ),이장희 ( Jang Hee Lee ),류범용 ( Buom Yong Ryu ) 한국조직공학과 재생의학회 2014 조직공학과 재생의학 Vol.11 No.1s

The annual regrowth of deer antlers is a connatural developmental event in mammals. Therefore, studying regeneration of deer antlers could be a unique natural model of rapid and complete bone regeneration in human and other mammals. However, little is known about culture conditions and regulatory factors that stimulate growing of deer antler cells in vitro. The aim of this study was to enhance an in vitro culture efficiency of mesenchymal stem cells (MSCs) derived from deer antlers. In order to improve the culture condition, we selected minimal essential medium alpha (MEMα) as a basal medium and investigate whether serum could stimulate growing in these cells in basal medium in a dose-dependent manner. Next, to investigate the optimal temperature and O2 tension, the antler cells were cultured in different temperature and controlled O2 percentages. Through the results of number of harvested cells after 1 week, we selected MEMα, 10% fetal bovine serum (FBS), 37oC, 20% O2, and 5% CO2 tension as a basic culture conditions. Also, we could observed enhanced proliferation results by addition of the supplements [L-glutamine 2 mM, β- mercaptoethanol 100 μM, non-essential amino acid (NEAA) 0.1 mM, and HEPES 10 mM] and growth factors [basic fibroblast growth factor (bFGF) 10 ng/mL, epidermal growth factor (EGF) 20 ng/mL, insulin-like growth factor-1 (IGF-1) 10 ng/mL] and harvested antler cells strongly expressed STRO-1 and CD 90. Our results demonstrate that allow continuous proliferation of antler cells in vitro established the foundation to basic biology of antler cells and makes possible application to the regenerative medicine in a broad sence.

조, 기장, 수수를 이용한 식혜의 이화학적 특성

정미선(Mi Seon Jeong),고지연(Jee Yeon Ko),송석보(Seuk Bo Song),이재생(Jae Saeng Lee),정태욱(Tae Wook Jung),윤영호(Young Ho Yoon),오인석(In Seok Oh),우관식(Koan Sik Woo) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.11

잡곡을 이용한 식혜의 제조 적성을 확인하기 위해 조, 기장, 수수를 이용하여 식혜를 제조하고 제조된 식혜의 이화학적 특성을 확인하였다. 잡곡 원료의 일반성분은 멥쌀과 찹쌀에 비해 수분 함량은 적지만 회분과 조단백, 조지방 함량은 높았다. 원료곡의 총 폴리페놀 및 플라보노이드 함량은 동안메가 일품과 백옥찰보다 2배 이상 높았고, DPPH 및 ABTS radical 소거 활성 또한 동안메가 높게 나타났다. 잡곡별로 제조된 식혜의 pH에서는 유의적인 차이가 없었으며, 탁도는 대부분 0.5 이하의 값을 나타낸 반면 동안메는 1.07로 높게 나타났다. 당도는 찹쌀과 멥쌀에 비해 낮았으며 기장, 수수, 조 순으로 나타났다. 명도와 적색도는 동안메가 가장 높았고, 황색도는 노란색을 띠는 삼다메가 높은 수치를 보였다. 식혜의 총 폴리페놀 함량은 동안메와 삼다메 식혜가 각각 258 및 241 μg GAE/mL로 높게 나타났으며, 플라보노이드 함량은 동안메 식혜가 54.30 μg CE/mL로 높았다. 식혜의 DPPH radical 소거 활성은 동안메가 16.85 mg TE/100 mL로 다른 잡곡류에 비해 2배 이상 높았으며, 백옥찰과 일품쌀에 대해서는 약 10∼15배로 높은 함량을 나타내었다. ABTS radical 소거 활성은 동안메가 가장 뛰어났으며 삼다 메가 그 뒤를 이었다. 따라서 잡곡 원료 간의 일반성분 함량 차이는 크지 않았으며, 잡곡 원료와 잡곡 식혜에서 동안메로 제조한 식혜가 가장 항산화력 및 radical 소거능이 뛰어났음을 알 수 있었다. 더불어 잡곡으로 제조한 식혜의 관능평가에서는 삼다메가 일품과 백옥찰에 색과 향에 유사한 선호도를 나타내었기에 동안메를 중심으로 레시피를 보완하여 식혜로 개발하는 것이 적합한 것으로 판단된다. This study was carried out to compare the physicochemical characteristics and sensory qualities of Sikhye(Korean traditional rice beverage) prepared with foxtail millet, proso millet, and sorghum. The cultivated varieties were Setaria italica Beauv. cv. Samdamae (SDM) and Samdachal (SDC), Panicum miliaceum L. cv. Ibaekchal (IBC), Sorghum bicolor (L.) Moench cv. Nampungchal (NPC), Donganme (DAM), Oryza sativa cv. Ilpum (IP), and Baegokchal (BOC). The brix degrees of SDM, SDC, IBC, NPC, DAM, IP, and BOC Sikhye were 9.53, 8.63, 5.67, 7.57, 6.27, 12.50, and 12.27°Bx, respectively. There were no significant differences in pH (5.99~6.10) among the groups. The highest turbidity was 1.07 in DAM Sikhye. The L-value, a-value, and b-value were 30.85~41.11, -0.34~2.52, and 2.56~5.67, respectively. Total polyphenol contents of SDM, SDC, IBC, NPC, DAM, IP, and BOC Sikhye were 241.52, 213.69, 202.34, 258.25, 193.24, 160.81, and 170.31 μg GAE/mL, respectively. Total flavonoid contents of Sikhye were 19.21~54.30 μg CE/mL. The highest DPPH and ABTS radical scavenging activities were 16.85±0.39 and 64.75±2.92 mg TE/100 mL in DAM Sikhye, respectively. Finally, the sensory evaluation results indicate that there were significant differences in appearance, aroma, and taste between the groups, and SDM Sikhye was similar with IP and BOC.

Improvement of Runner Plant Production by Increasing Photosynthetic Photon Flux during Strawberry Transplant Propagation in a Closed Transplant Production System

김성겸,정미선,박선우,김무정,나해영,전창후,Kim, Sung-Kyeom,Jeong, Mi-Seon,Park, Seon-Woo,Kim, Moo-Jung,Na, Hae-Young,Chun, Chang-Hoo Korean Society of Horticultural Science 2010 원예과학기술지 Vol.28 No.4

국내 육성 품종인 '매향' 딸기의 육묘 기간 중 광합성유효광량자속의 환경 조절을 통하여 러너 및 러너플랜트의 발생과 생산 효율을 증대시키고자 하였다. 딸기 육묘에 이용된 증식체의 엽수와 관부 직경은 각각 $3.1{\pm}0.4$와 $7.0{\pm}1.1mm$였다. 광합성유효광량자속을 각각 140, 210 및 $280{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$로 설정한 9기의 인공광 이용형 육묘 모듈에서 35일 동안 육묘하였다. 러너플랜트 생산 효율은 증식체당 일일 0.27주로 처리구 중 유의하게 가장 높았으며 관행의 딸기 육묘 방식의 러너플랜트 생산 효율과 비교하여도 크게 증진되었음을 확인하였다. 폐쇄형 육묘 시스템을 활용하여 육묘 기간 중의 PPF를 $280{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$로 조절하면 국내 육성 품종인 '매향'의 러너 발생 및 러너플랜트 생산을 증진시킬 수 있음이 확인되었다. 따라서 최근 국내에서 육성된 신품종 딸기의 급속 보급을 위한 증식체계 구축에 본 연구 기술이 활용되면 육성된 품종의 조급 보급이 가능할 것으로 판단된다. The formation and elongation of runners, growth of runner plants, and transplant propagation rates of 'Maehyang' strawberry were investigated at various photosynthetic photon flux (PPF) levels. Strawberry plants having $3.1{\pm}0.4$ leaves and $7.0{\pm}1.1mm$ of crown diameter were used as propagules and were cultured for 35 days in 9 transplant production modules using fluorescent lamps as artificial lighting sources. Applied PPF levels were $137.4{\pm}2.1$, $217.0{\pm}1.0$, and $274.7{\pm}8.4{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ as measured on the surfaces of empty shelves. The numbers of runners and runner plants per propagule were the greatest at $280{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPF. The runner plant propagation rate was 0.27 plant/day/propagule at $280{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, which was significantly greater than that of conventional propagation methods. Results indicate that high PPF levels promotes the formation of runners and runner plants of strawberry and that the rapid propagation method with high PPF levels can be feasible for production of vigorous transplants in a closed transplant production system.

가스 차단기에서 발생하는 비정상 압축성 유동의 수치해석적 연구

정성재(Sung-Jae Jung),정미선(Mi-Seon Jeong),김희동(Heuy-Dong Kim) 대한기계학회 2002 대한기계학회 기타 간행물 Vol.2002 No.11