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한국 토양균중 Leucine aminopeptidase 저해제의 검색 및 분리에 관한 연구(제1보)
전정순(Jeong Soon Jeon),박설희(Seol Hee Park),최응칠(Eung Chil Choi),김병각(Byong Kak Kim) 한국생약학회 1987 생약학회지 Vol.18 No.1
To find microorganisms producing leucine aminopeptidase inhibitors, microbes were isolated from soil samples that were collected at different locations in Korea and screened for inhibitory activity. Strain DMC-454 which was found to show the inhibitory activity was very similar to Streptomyces griseofuscus. It was grown in a liquid medium containing oatmeal, yeast extract and mineral salts. The highest inhibitory activity was obtained by the three-day old culture. The inhibitor of this strain was purified by means of adsorption on Amberite XAD-4 and column chromatography on Amberlite CG-120 and gave a single spot at Rf 0.36 with n-butanol-acetic acid-distilled water (4:1:1). The mode of the enzyme inhibition was competitive type.