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박인석,임성영,이태호,길현우,유광열 한국발생생물학회 2017 발생과 생식 Vol.21 No.2
A comparison of the growth, hematological values, fatty acids, and gonadal and growth hormonal changes of river puffer, Takifugu obscurus, tiger puffer, T. rubripes, their hybrids (river puffer × tiger puffer) and hybrid triploids was per-formed during 3 months of their early growth period. Several features were observed during these 3 months: hybrids showed the highest levels of specific growth rate, 1.48%; hybrid triploids showed the smallest change in viscera fat (P<0.05), but GSI was not significantly different among groups (P>0.05). Considering hematological parameters, hybrid triploids had increased mean corpuscular volume and mean corpuscular hemoglobin (P<0.05), but other parameters were not significantly different between groups (P>0.05). With respect to fatty acids, puffer fish, hybrids and hybrid triploids contained fatty acids such as SFAs, MUFAs, n-3 PUFAs and n-6 PUFAs. There were significantly different amounts of total fatty acids between groups (P<0.05), however, rates of changes in fatty acids did not differ significantly between groups (P>0.05). Gonadal hormone (es-tradiol and testosterone) changes in the river puffer and tiger puffer were significantly higher than that observed in hybrids and hybrid triploids. The hybrids and tiger puffers had higher amounts of growth hormone (thyroid stimulating hormone and thyroxine) than the hybrid triploids and river puffers (P<0.05).
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Salmonella typhimurium의 혐기적 산내성도 평가
김영찬,이선,이경미,임성영,박용근,백형석,박경량,이인수,Kim, Young-Chan,Lee, Sun,Lee, Kyung-Mi,Im, Sung-Young,Park, Yong-Geun,Baek, Hyung-Seok,Park, Kyung-Ryang,Lee, In-Soo 한국생명과학회 1999 생명과학회지 Vol.9 No.2
Salmonella typhimurium can encounter a wide variety of environments during its life cycle. In nature, S. typhimurium can experience and survive dramatic acid stresses that occur in diverse ecological niches ranging from pond water to phagolysosomes. These survival mechanism is aquired by the Acid Tolerance Response(ATR) in Salmonella. The ATR of S. typhimurium is a complex inducible phenomenon in which exposures to slight or moderate low pH will produce a stress response capable of protecting the organism against more severe acid challenges. ATR in Salmonella has two different systems that are called RpoS dependent and independent. We found that ATR in anaerobic was showed RpoS independent because rpoS$\Omega$AP had ATR as S. typhimurium UK1. Using the P22 MudJ(Km, lacZ) operon fusion technique and a lethal selection procedure combining low pH(pH4.5) and sodium acetate(10mM, pH4.5), we isolated LF487 aatA::MudJ which showed acid sensitive in anaerobic condition. aatA locus was determined at 12 min on Salmonella Genetic Map. The survival rate of aatA mutant was showed significantly diminished at pH4.3 than virulent wild type Salmonella in anaerobic condition(5% $CO_2$, 5% H$_2$, 90% $N_2$). Therefore isolated gene was confirmed important gene for anaerobic ATR system.
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병원성 Salmonella enterica serovar Typhimurium의 저온 유도성 산 내성 반응
송상선,이선,이경미,임성영,조민호,박용근,박경량,이인수,Song, Sang-Sun,Lee, Sun,Lee, Mi-Kyoung,Lim, Sung-Young,Cho, Min-Ho,Park, Young-Keun,Park, Kyeong-Ryang,Lee, In-Soo 한국미생물학회 2001 미생물학회지 Vol.37 No.3
The acid tolerance response (ATR) of log-phase Salmouella enterica seroyar Typhimurium is induced by acid adaptation below pH4.5 and will protect cells against more severe acid. Two distinctive ATR systems in thisorganism are a log-phase and stationary-phase ATR in which acid adaptations trigger the synthesis of acid shockproteins (ASPs). We found that log-phase ATR system was strongly affected by environmental factor, low tem-perature, $25^{\circ}C$. Exposure to low temperature and mild acid has been shown to increase acid survival dra-matically, and this survival rate was showed higher than $37^{\circ}C$. Especially unadapted cells at $25^{\circ}C$ presented tenthousand folds survival increasing when compared with cells at $37^{\circ}C$. The degree of acid tolerance of rpoSwhich is blown to be required for acid tolerance more increase than $37^{\circ}C$. Even though AIR pattern of rpoSbetween unadapted and adapted was showed similar at pH 3.1, rpoS-dependent ATR system also has beendetected in low temperature because rpoSAp prevents sustained acid survival at $25^{\circ}C$. Therefore the resultssuggest low temperature ATR system requires rpoS-dependent and -independent both. To investigate the basisfor low temperature related ATR system, gene that was participated for low temperature acid tolerance (lat) wasscreened in virulent S. enterica serovar Typhimurium UKl Using the technique of P22- MudJ (Km, lacZ)-directed lacZ operon fusion, LF452 latA‥‥MudJ was isolated. The latA‥‥MudJ of S. enterica Typhimurium pre-vented low temperature acid tolerance response. Therefore latA is considered one of the important genes for acidadaptation.
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박원규(Won Kyu Park),이종상,선치성,권선민,임성영,송지석,김태운 대한교통학회 2008 대한교통학회 학술대회지 Vol.58 No.-
2006년 한해 발생한 213,745건의 교통사고 중 차 대 보행자 사고는 21.2%인 45,261건이고, 전체 사망자 6,327명 중 차 대 보행자 사고에서 사망한 보행자는 37.6%인 2,377명으로 전체 사고 비율에 비해 차 대 보행자 사고로 사망을 당한 보행자가 많은 중대한 교통사고이며, 뺑소니 사고는 1974년 전체사고의 3.1%를 차지하는 1,331건에서 1984년에는 전체사고의 2.4%인 3,178건이고 2006년에는 6.7%인 14,480건으로 매년 뺑소니 사고가 질 및 양적으로 증가되는 추세임에도 불구하고, 차 대 보행자 사고 시 사고 운전자는 보행자를 충돌(역과 포함) 시 인지를 하지 못하였다는 진술을 할 경우 이를 규명할 과학적인 자료 부족(진동 및 소음 정도)으로 인하여 형사적 책임 및 실체적 진실 규명에 어려움이 봉착된 상황임. 현재 확인된 연구자료에는 인체 충돌 및 역과 시 발생하는 현상(진동 및 소음)에 대한 공인 및 실험된 자료가 없어 사고 원인 분석 및 실체적 진실 규명에 문제점이 제기되는 바, 본 연구에서는 인체 역과 시 발생하는 진동 및 소음을 심층 연구하여 차 대 보행자 사고(인지 여부)에 적용 가능한 연구자료(진동 및 소음도)를 제시하고자 한다.
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유한요소해석을 통한 선재의 표면 흠이 차량용 엔진 밸브 스프링 수명에 미치는 영향 평가
안남식(Nam-sik Ahn),이경훈(Kyung-hun Lee),김병민(Byung-min Kim),홍리석(Lee-Seok Hong),정진영(Jin-young Jung),임성영(Sung-young Lim) 한국자동차공학회 2012 한국자동차공학회 지부 학술대회 논문집 Vol.2012 No.5-1
Recently, the high surface quality of oil hardened and tempered spring steel wire for valve springs with high fatigue strength has been required for the fuel economy of automobile engines. The aim of this study is to investigate the fatigue life of advance high strength steel(AHSS) springs according to surface flaws of oil hardened and tempered spring steel wire. V-shape surface defects were introduced in the initial workpiece and the evolution of deformation behavior and stress distribution such defects was simulated during the manufacturing spring, The FE-simulations were performed to check the local stress distributions around surface flaws using an AHSS spring, which are determined by analysis results of cold coiling and measured residual stresses. The fatigue lives were predicted in comparison with S-N curves and calculated alternating stress.
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