The Antioxidative Activity of Glutathione-Enriched Extract from Saccharomyces cerevisiae FF-8 in In Vitro Model System

이치형,차재영,전방실,이호준,이영춘,최용락,조영수,Lee Chi-Hyeoung,Cha Jae-Young,Jun Bang-Sil,Lee Ho-Jun,Lee Young-Chun,Cho Yong-Lark,Cho Young-Su Korean Society of Life Science 2005 생명과학회지 Vol.15 No.5

The Antioxidative accvities of the cell free extracts containing high glutathione by Saccharomyces cerevisiae FF-8 were tested in vitro experimental models : DPPH method for radical scavenging activity, ferric TBA method and ferric thiocyanate method using linoleic acid and tissue microsome for lipid peroxidation inhibitions. The concentration of intercellular glutathione by cultivating S. cerevisiae FF-8 in the YM optimal medium obtained $204\mug/ml$, which was increased by 2.76-fold from $74\mug/ml$ in the YM basal medium. A comparition between the YM basal medium and the YM optimal medium on antioxidative substance produced by S. cerevisiae FF-8 was investigated. In DPPH ($\alpha, \alpha-diphenyl-\beta-picrylhydrazyl$) method, the electron donating activity of the glutathione produced by S. cerevisiae FF-8 cultured in the YM optimal medium was as high as that of BHT ($ 0.05\%w/v $). The antioxidative a.tivity was measured by inhibition against lipid peroxidation of rat tissues' microsomes. The results of anti-oxidant activity of the cell free extracts by S. rerevisiae FF-8 cultured in the YM optimal medium was shown in the following order . $ liver 60.98\% > kidney 56.43\% > heart 52.91\% > brain 52.13\% > testis 45.57\% > spleen 42.95\% $. In antioxidative activities determined by ferric thiocyanate method and TBA methods against lipid peroxidation, the lipid peroxidation in the control mixture increased more rapidly than the typical peroxidation curve of linoleic acid from one day. The antioxidative activity of the cell free extracts by cultivating S. cerevisine FF-8 in the YM optimal medium were higher than that of the YM basal medium. These data indicate that the cell free extracts containing a high intercellular glutathione of S. cerevisiae FF-8 cultured in YM optimal medium showed strong antioxidative capacities by DPPH radical scavenging activity and ferric thiocyanate and TBARS measurements.

Relationship between Alcohol Use Disorders Identification Test Fractional Anisotropy Value of Diffusion Tensor Image in Brain White Matter Region

이치형,김경립,곽종혁,Lee, Chi Hyung,Kim, Gyeong Rip,Kwak, Jong Hyeok The Korean Society of Radiology 2022 한국방사선학회 논문지 Vol.16 No.5

Magnetic resonance diffusion tensor imaging (DTI) has revealed the disruption of brain white matter microstructure in normal aging and alcoholism undetectable with conventional structural MR imaging. we plan to analyze the FA measurements of the ROI of dangerous drinkers selected from Alcohol Use Disorders Identification Test (AUDIT) and Tract-Based Spatial Statics (TBSS) tool was used to extract FA values in the ROI from the image acquired through the pre-processing process. TBSS has a higher sensitivity of the FA value and MD value in the white matter than the brain gray matter, and has the advantage of quantitatively deriving the unlimited degree of brain nerve fibers, and more specialized in the brain white matter. We plan to analyze the fractional anisotropy (FA) measurement value for damage by selecting the center of the anatomical structure of the white matter region of the brain with high anisotropy among the brain neural networks that are particularly vulnerable to alcohol as the region of interest (ROI). In this study, we expected that alcohol causes damage to the brain white matter microstructure from FA value in various areas including both Choroid plexus. Especially, In the case of the moderate drunker, the mean value of FA in Lt, Rt. Choroid plexus was 0.2831 and 0.2872, whereas, in the case of the severe drunker, the mean value of FA was 0.1972 and 0.1936. We found that the higher the score on the AUDIT scale, the lower the FA value in ROI region of the brain white matter. Using the AUDIT scale, the guideline for the FA value of DTI can be presented, and it is possible to select a significant number of potentially severe drinkers. In other words, AUDIT was proved as useful tool in screening and discrimination of severe drunker through DTI.

개방형 콘텐츠 유통환경에서 시장집중 규제에 대한 비판적 고찰

이치형(Chi Hyung Lee),이중만(Jungmann Lee),이종원(Jongwon Lee) 한국콘텐츠학회 2012 한국콘텐츠학회논문지 Vol.12 No.11

전통적으로 미디어 시장은 사업자수가 적고 특정 미디어의 영향력이 크기 때문에 시장집중을 엄격하게 규제하여 왔다. 하지만 미디어에 개방형 인터넷이 도입됨에 따라 미디어 규제의 근거인 희소성과 침투성 개념이 약해지고 있다. 본 연구는 미디어 융합 환경에서 기존 시장집중 규제의 문제점을 비판적으로 평가한다. 개방형 콘텐츠 유통구조에서 시장성과는 시장구조보다 사업자행위에 더 영향을 받으며, 수직결합이 서비스 혁신의 수단으로 부각되고 있고, 시장획정이 끊임없이 변하기 때문에 시장집중 측정이 어려워지고 있다. 따라서 시장집중은 단순히 사업자의 시장점유율과 같은 구조적 요인으로만 규제할 것이 아니라 시장비효율과 다양성을 저해하는 사업자 행위를 중심으로 규제하는 것이 더 적합하다는 결론이다. Government has strictly regulated the concentration of the media market because the number of broadcasters was traditionally limited and they had significant influence on public. However as the media market converges with Internets open architecture, scarcity and pervasiveness are losing their roles in justifying media regulation. This article examines market convergence and its impact on market concentration, and criticizes the current concentration regulation. The study indicates that market performance are much governed by firms competitive actions than market structure, and that vertical integration contributes to service innovations, and that horizontal concentration makes it difficult to measure due to its even-changing market definition. Therefore, the regulation for market concentration should shift its focus from firms market share to broadcasters unfair acts driving market inefficiency and hurting content diversity.

난소 및 자궁부속기 염전의 보존적 치료를 위한 색도플러 초음파검사의 의의

이치형(CH Lee),황경주(KJ Hwang),장기홍(KH Jang),권혁찬(HC Kwon),유희석(HS Ryu),이은주(EJ Lee),이숙영(SY Lee),오기석(KS Oh) 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.7

Torsion of the adnexa is a well-known gynecologic cause of an acute surgical abdomen. Delay in diagnosis, inability to distinguish strangulation from necrosis, and fear of embolus dislodgement have made adnexectomy the accepted method of management of adnexal torsion. This condition occurs most commonly in the reproductive years, yet methods to preserve viable ovarian tissue have not been routinely used or evaluated. Therefore, in order to ascertain if color Doppler sonography(CDS) can detect adnexal viability, ultrasonography with CDS of the ovarian pedicle was performed in 27 patients in whom torsion of the ovarian tumor was confirmed surgically. We were able to identify a twisted vascular pedicle of the ovarian tumor by ultrasonography in 24 of 27 patients(88% detectability). In 10 patients in which pedicle arterial and venous blood flow was observed, the pathology specimens revealed normal, or edema with congestion, or early hemorrhage, but in the 9 cases where only arterial blood flow was observed or where there was no blood flow at all, pathology revealed hemorrhagic necrosis in all cases. In 5 cases where there was arterial blood flow the tumor was managed conservatively, either by detorsion or cytectomy, after which there was no cases of embolism or tumor recurrence during follow up ultrasonography. Normal follicular development and ovulation was also observed in these patients. In conclusion, for young women who are of child-bearing age in whom torsion of benign adnexal tumors is suspected, CDS should be conducted to detect torsion of the tumor and ascertain whether pedicle venous blood flow is present or not. If such blood flow is detected, the adnexa is considered to be viable and detorsion or cystectomy may be performed, thus preserving the ovary.

택시 호출앱 등장과 택시서비스 노동의 변화

이치형 ( Chi-hyung Lee ) 사회혁신기업연구원 2021 혁신기업연구 Vol.6 No.2

Unlike traditional technologies automating manual works in the manufacturing sectors, digital technologies such as artificial intelligence and robot equipped with cognitive capabilities automate both low-skilled and high-skilled works in the service sectors. Innovative technologies tend to re-organize a variety of works within a job rather than replacing the whole one at the initial stage. This study aims to analyze the process of re-organizing existing works and its consequent change of labor in the taxi service since Taxi call-app came to the market. The matching, navigation, payment and evaluation systems in the application substitute most of human's labor except physical driving. Consequently, the existing labor is under the deskilling process although the number of drivers doesn't decrease while new high-skill labor emerges to support intelligent taxi service. The analysis implies that flexibility of labor market and corporate strategy affect quality and quantity of labor in short-term, and technologies promote labor decomposition and integration to innovate service, and deskilling is likely to lead to the decrease of wage. Additional empirical research is needed when industry statistics become available.

고도 정제된 인간의 FSH[highly purified FSH , FSH-HP]를 사용한 단순화된 체외수정 및 배아이식술의 효용성

이치형(CH Lee),황경주(KJ Hwang),유정현(JH Yoo),권혁찬(HC Kwon),장기홍(KH Jang),김지영(JY Kim),오기석(KS Oh) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.5

Objectives : To compare simplified ovulation induction employing FSH-HP with that of simplified ovulation induction employing other pharmacologic agents with regard to ovulation induction characteristics, oocyte and embryo development, and pregnancy outcome. Methods : From December 1, 1994 to August 31, 1997, those cycles who underwent in vitro fertilization and embryo transfer with FSH-HMG [84 cycles], GnRH agonist short protocol [142 cyclse] and long protocol [175 cycles] and FSH-HP [103 cycles], totalling 504 cycles. Results : The duration of ovulation induction with FSH-HP was 7.6±0.2 days, which was significantly shorter than the 9.0±0.1 days when GnRH agonist was used, and the total dose of agents were also significantly less [26.4±1.1 ampules vs 34.8±1.2 ampules]. The incidence of premature luteinization, cycle cancellation rate, and OHSS development was similar between the two groups. In the FSH-HP group good endometrial response was observed. The serum estradiol concentrations were significantly lower than the GnRH agonist short and long protocols [p<0.05], but there was normal oocyte and embryo development. Rather, although there was no statistical significanse, the implantation rate was relatively higher whereas the abortion rate was relatively lower, leading to higher ongoing pregnancy rate overall. Conclusion : Ovulation induction with FSH-HP enables subcutaneous drug administration by the patient herself, therefore simplifying as much as possible the in vitro fertilization and embryo transfer process and affording conveniency for both the patient and the hospital. It is therefore suggested that simplified protocols in IVF-ET with highly purified FSH [FSH-HP] for ovulation induction is an efficient alternative to current standard protocols.

신념의 형성과 사육신의 충절: 도덕교육의 성격과 과제에 고함

이치형(Chi-Hyung Lee) 한국도덕교육학회 2021 도덕교육연구 Vol.33 No.2

The purpose of this article is to explore the spirit of the deceased through the ethnic historical perspective of Danjae Shin Chae-ho, revealing the implications of moral education and its challenges. According to this study, the theoretical aspect of moral educa―tion must be derived along with the practical aspect, and when it does, it can be a theory of life to live reality. Therefore, the nature of moral education takes on the task of performing its role as a theory of life, which is interpreted as meaning that theory and practice develop on the basis of exquisite balance rather than separately in moral education. Therefore, a person who studies moral education soon has the meaning of self-regulation (autonomy) that his life is supported by the methodical principles of moral education every moment, which is not separate from practicing the life of departing to reality.

교과와 수업: 불교 인식론적 관점

이치형 ( Chihyung Lee ) 한국교육과정학회 2020 교육과정연구 Vol.38 No.3

This study explores the internalization process of a classroom subject from the perspective of epistemology, particularly Buddhist epistemology. Buddhist epistemology can be presented by dividing the surface and deep levels. From this point of view, it is possible to explain a subject’s internalization process more specifically in relation to deeper perception because the system can be explained as a dynamic process through the interaction between surface and deep perceptions. Furthermore, it is easier to explain the subject in relation to the world and the mind by the Buddhist epistemology based on the Alaya-Vijnana System, and this understanding will contribute to solving the controversy about ‘the first knowledge,’ which has been called Meno’s Paradox and has been challenging when explaining a subject’s learning-internalization. Also, by the curriculum establishing a class as the interaction between the teachers’ and students’ perceptions of the complex interaction between surface and deep perceptions, it is possible to explain class dynamics more specifically, which will be an opportunity to explain the relationship between curriculum education and personality education.

원효 ‘삼세·아려야식설’ 창안의 교육학적 의미

이치형(Lee, Chi Hyung) 한국교육철학회 2021 교육철학 Vol.80 No.-

The purpose of this article is to interpret Wonhyo s three subtle[三細]·ālaya vijñāna[阿賴耶識] theory educationally. Thus, this study deduced what educational implications are by grasping Wonhyo s theory of mind from a more matched point of view. According to this paper, education is the most important thing to cultivate the mind, and it should be the reason why students acquire knowledge of beautiful mind, and it is related to not only cultivating their own life but also grasping the fundamental meaning of their job in relation to their job more directly. Thus, it was found that students need to approach their jobs from the perspective of a great mind rather than a child while learning at school.

해외유료방송의 시장집중과 시장성과 간의 관계분석을 통한 미디어산업 규제 및 정책 방향

이치형 ( Chi Hyung Lee ),이봉규 ( Bong Gyou Lee ) 한국인터넷정보학회 2011 인터넷정보학회논문지 Vol.12 No.4

The purpose of this study is to provide relevant directions of media industry regulation and policy by analyzing the relationship between market concentration and performance in global pay TV markets. The market concentration has long been regulated in the media industry under the belief that it harms market efficiency and cultural diversity. As the global media market is undergoing significant chance accelerated by the advance of technology, it is necessary to examine the feasibility of this long myth. The data are collected from global 17 pay TV markets and analyzed to see whether the highly concentrated markets by a single player result in higher price and slower service penetration. The result indicates that the price is not escalated and the penetration is not limited even in the markets where a dominant player takes high market share. It implies that more diverse factors other than the market concentration affect the market performance and that maintaining strict regulation of the market concentration is likely to have negative effect on technology innovation.