겔형태 인공누액이 각막실질세포 및 결막상피세포에 미치는 영향

이종수,김은희,김수진,이지은,김나미,Jong Soo Lee,Eun Hee Kim,Soo Jin Kim,Ji Eun Lee,Na Mi Kim 대한안과학회 2010 대한안과학회지 Vol.51 No.12

Purpose: To evaluate the biological effects and cytotoxicity of gel-type artificial tears on human corneal keratocytes and conjunctival cells in vitro. Methods: Human corneal keratocytes and conjunctival epithelial cells were exposed to Soothe® and Systane® at variable concentrations. Evaluations were conducted through an MTT-based calorimetric assay to measure the metabolic activity and through a lactate dehydrogenase (LDH) assay to assess cellular damage. Apoptotic response was examined using fluorescent microscopy and flow cytometric analysis, and cellular morphologic results were evaluated with a transmission electron microscope. Results: The inhibitory effects of corneal keratocyte and conjunctival cell proliferations increased at higher concentrations and longer exposure times to Soothe® and Systane®. The LDH titers increased after Soothe® exposure, but showed no significant difference after Systane® exposure. Soothe® and Systane® treatments both produced fluorescence, representing apoptotic cells. In flow cytometry, the maximal apoptotic response was observed for both types of artificial tears, although Systane® showed less edema, as well as reduced cytoplasmic and nuclear cell degeneration compared to those of Soothe®. Conclusions: The apoptotic responses of Soothe® and Systane® are associated with inhibitory effects of human corneal keratocyte and conjunctival epithelial cell proliferations. To inhibit the cellular proliferation of human corneal keratocytes and conjunctival epithelial cells, Systane® may be less severe than Soothe® at higher concentrations and longer exposure times. J Korean Ophthalmol Soc 2010;51(12):1643-1651

정상 및 원추각막에서 각막 전, 후면의 융기 및 시상면만곡도의 비교분석

이종수,이승욱,이승민,이미현,Jong Soo Lee,Seung Wook Lee,Seung Min Lee,Mi Hyun Lee 대한안과학회 2012 대한안과학회지 Vol.53 No.10

Purpose: To compare changes of anterior and posterior corneal elevation, and sagittal curvature according to the severity of keratoconus and to compare differences between keratoconus and normal cornea. Methods: A total of 81 eyes diagnosed with keratoconus and 20 eyes of normal subjects were evaluated with a Pentacam Scheimpflug camera. The keratoconus eyes were divided into 3 groups according to mean keratometer (K): mild (K ≤ 47.0 diopters (D)), moderate (47.0 to 52.0 D), and severe (52.0 D≥). The following parameters were obtained to evaluate the correlation of keratoconus: corneal thickness, anterior and posterior corneal elevation, and sagittal curvature. Results: Out of 81 keratoconus eyes, 56 eyes were mild, 12 eyes were moderate, and 13 eyes were severe keratoconus. The mean central corneal keratometer, anterior and posterior corneal elevation, and sagittal curvature of the keratoconus eyes were 49.7 D, 22.07 μm, 38.16 μm, 52.76 D and the values increased statistically compared to the normal eyes. Furthermore, the values increased significantly with the severity of keratoconus. ROC curve analysis showed the estimated meaningful value for anterior and posterior corneal elevation and sagittal curvature of keratoconus for diagnosis; there were no diagnostic values for corneal thickness and refractive power. Conclusions: The index of 5.5 μm for mean anterior elevation, 12.5 μm for mean posterior elevation, and 44.5 μm for mean sagittal curvature using the PentacamⓇ are useful to diagnose keratoconus. Variation of anterior and posterior elevation, and sagittal curvature measured by PentacamⓇ are useful in understanding the process of keratoconus. J Korean Ophthalmol Soc 2012;53(10):1432-1437

익상편조직의 섬유모세포에 미치는 사이클로스포린의 영향

이종수,이승욱,이상준,김나미,Jong Soo Lee,Seung Wook Lee,Sang Jun Lee,Na Mi Kim 대한안과학회 2012 대한안과학회지 Vol.53 No.3

Purpose: To evaluate the response and cellular damage of cultured human pterygial cells according to the concentration and exposure time of topical cyclosporin. Methods: Human pterygial cells were exposed to a cyclosporin A concentrations of 0.1 μg/ml (0.0001%), 1 μg/ml (0.0001%), 10 μg/ml (0.001%), 100 μg/ml (0.01%), or 500 μg/ml (0.05%) for 5 or 10 minutes. An MTT-based colorimetric assay was performed to assess the metabolic activity of cellular proliferation, and a lactate dehydrogenase (LDH) leakage assay was used to determine cellular damage. The extra-cellular matrix of PIP, laminin and MMP were evaluated, and the measurement of pro-inflammatory cytokine, TNF-α and IL-1b. IL-6, IL-8 was performed using ELISA kits. Results: The pterygial cellular inhibitory effect of cyclosporin was similar to that of the control according to the concentration and exposure time (p > 0.05). Compared with the control, the level of LDH did not show a statistically significant difference between concentration and exposure time (p > 0.05). There was no significant difference of inhibitory effects by PIP, laminin, or MMP between the experimental and control groups (p > 0.05). The production of TNF-α and IL from the experimental pterygial cells due to the effect of cyclosporin was not significantly different from that of the control at a longer exposure time or stronger concentration (p > 0.05). Conclusions: The response of pterygial cells to topical cyclosporin A at concentrations less than 0.05% for less than 10 minutes of exposure time showed no prevention of pterygial recurrence. With regard to cellular damage, little effects on inhibition of PIP, laminin, MMP, IL, and TNF-α were observed compared with that of the control. J Korean Ophthalmol Soc 2012;53(3):466-472

몇종의 세균과 Saccharomyces cerevisiae에 대한 식품첨가물의 향균 특성

이종수,오준세,김나미,금종화,이석건,Lee, Jong-Soo,Oh, Jun-Sei,Kim, Na-Mi,Keum, Jong-Hwa,Lee, Suk-Kun 배재대학교 자연과학연구소 1996 自然科學論文集 Vol.8 No.2

현재 식품첨가물로 많이 사용되고 있는 유기산과 안정제 및 색소 등의 각종 세균과 Sacch. cerevisiae 에 대한 향균 특성을 조사하였다. 산도 조절용으로 사용되고 있는 젖산, 사과산, 호박산 및 주석산은 L. acidophilus와 Sacch. cerevisiae에 대하여 향균성이 없었으나 B. subtilis등의 세균에 대하여는 향균성이 있었고 특히 사과산은 P. aeruginosa에 대하여 강한 향균력이 있었다.(최소생육저지농도 : 0.05%). 안정제로서의 알긴산과 펙틴은 B. subtilis, E. coli, P. aeruginosa에 대하여 비교적 강한 향균성을 보였고 L. acidophilus에 대하여는 향균성이 없었다. 황색 색소(홍화엘로우)와 적색 색소(Red powder-N)는 향균성이 없었고 표백제인 $NaHSO_3$의 세균에 대한 최소생육저지농도는 0.05%, Sacch. cerevisiae에 대하여는 0.5%로 향균성이 있었다. In order to survey the safety of some food additives, antimicrobial activity of acidulants, stabilizers, antioxidants, natural coloring materials and bleaching agents against 5 strains of bacteria and Sacch. cerevisiae were investigated by dilution method and minimal inhibitory concentration(MIC) method. Malic acid as acidulants displayed the effective antimicrobial activity in vitro against P. aeruginosa and its MIC is 0.05%. Alginic acid and pectin as stabilizer also displayed strong antimicrobial activity against B. subtilis, E. coli and P. aeruginosa, and tannin(antioxidants) and $NaHSO_3$ displayed antimicrobial activity against all bacteria tested. However gums(Arabia, Xanthan, Gua) and natural coloring materials(Hongwha Yellow, Red powder-N) were not affected to growth of bacteria and Sacch. cerevisiae.

실사적인 3차원 물체 모델링 기법 개선

이종수,Lee, Jong-Soo 한국정보처리학회 2003 정보처리학회논문지B Vol.10 No.2

본 논문에서는 실사적인 3D 물체 모델링을 위해 제안된 기법들을 고찰하고, 보다 정확한 모델링을 위한 방안들을 제시하고 있다. F-행렬 추정기법의 개선과 스테레오 영상 평행화기법을 적용함으로 물체 모델링의 정확도를 높히는데 필수적임을 보여준다. In this paper, in-depth considerations are given to various techniques suggested for photographic 3D modelling and possible enhancements of the techniques are discussed. It is found that both the improvement of fundamental matrix estimation techniques and the stereo image rectification processing stage are necessary for more accurate 3D modelling.

β-galactosidase를 생성하는 고온성(高溫性) 세균(細菌)의 생리적(生理的) 특성(特性)에 관한 연구(硏究)

이종수,김찬조,Lee, Jong Soo,Kim, Chan Jo 충남대학교 농업과학연구소 1982 Korean Journal of Agricultural Science Vol.9 No.1

고온균(高溫菌)의 생리적(生理的) 특성(特性)과 내열기구(耐熱機構)에 관한 기초자료(基礎資料)를 얻고자 온천토양(溫泉土壤)에서 ${\beta}$-galactosidase를 생산(生産)하는 고온균(高溫菌)을 분리(分離)하여 동정(同定)하고, 몇가지 주요(主要)한 생리적(生理的) 특성(特性)을 검토(檢討)하였으며, 그의 균체지방산(菌體脂肪酸) 조성(組成)을 gas chromatography로 분석(分析)하여 다음과 같은 결과(結果)를 얻었다. 1. ${\beta}$-galactosidase를 생산(生産)하는 공시(供試) 균주(菌株)는 Thermus속(屬)으로 동정(同定)되었다. 2. 분리선정(分離選定)한 균주(菌株)의 최적(最適) 생육온도(生育溫度)는 $65^{\circ}C$이었고, $37^{\circ}C$에서 생육(生育)하지 않는 절대(絶對) 고온균(高溫菌)이었으며 최적(最適) pH는 6.5내외(內外)이었다. 3. NaCl에 대(對)한 내성(耐性)은 NaCl 1% 이상(以上)에서 생육(生育)하지 못했다. 4. 공시균주(供試菌株)의 항생물질(抗生物質) 내성(耐性)은 Penicillin G는 $10{\mu}g/m{\ell}$, Chloramphenicol은 $0.5{\mu}g/m{\ell}$이었다. 5. Vitamin 요구성(要求性)은 Ca-pantothenate와 pyridoxine-HCl을 절대적(絶對的) 생육인자(生育因子)로 요구(要求)하였고 niacin을 자극적(刺戟的) 생육인자(生育因子)로 요구(要求)하였다. 6. 공시균주(供試菌株)는 최대(最大) 흡수파장(吸收波長)이 420nm 부근(附近)의 색소(色素)를 생성(生成)하였고 그의 생성능(生成能)은 약(弱)하였다. 7. 공시균주(供試菌株)의 균체 지방산(脂肪酸) 조성(組成)은 palmitic acid 60.20%, lauric acid 11.80%, myristic acid 7.56%, behenic acid 4.25%, capric acid 1.77%, stearic acid 2.13%, arachidic acid 1.53%이었다. This experiment was carried out to elucidate the thermotolerant properties of a thermophilic bacterium which was isolated from soils of the hot springs area and selected for the ${\beta}$-galactosidase production. This bacterium was identified as a strain belong to the genus Thermus. Biochemical and physiological characteristics of this strain were studied, including the investigation of the fatty acid composition of its neutral fats. The results obtained were summarized as follows. 1. Optimal temperature and pH for growth of this strain were $65^{\circ}C$ and pH 6.5 respectively, and it was found to be an absolute thermophilic bacterium which could not grow at the temperature below $43^{\circ}C$. 2. No growth was obtained in the medium which contained more than 1.0% of sodium chloride. 3. The tolerable concentration of antibiotics were 10mg of penicillin G per ml of medium and 0.5mg of chloramphenicol per ml respectively. 4. This strain had auxotrophilic requirements for calcium-pantothenate and pyridoxin-HCl as an essential factor and for niacin as a stimulative factor. 5. Yellow pigment was released into the liquid culture of this strain, which showed maximum absorption at 420 nm. 6. Fatty acid composition of neutral fats of the strain was palmitic acid, 60.20%; lauric acid, 11.80%; myristic acid, 7.56%; behenic acid, 4.25%; capric acid, 1.77%; stearic acid, 2.13%; arachidic acid, 1.53%; and others unidentified, 10.7%.

미생물 일반 , 생리 및 대사 재래식 메주에서 분리한 효모들의 각종 효소활성과 가능성

이종수,이성훈,권수진,안철,유진영 ( Jong Soo Lee,Sung Hun Yi,Su Jin Kwon,Cheol Ahn,Jin Young Yoo ) 한국미생물생명공학회 1997 한국미생물·생명공학회지 Vol.25 No.5

Neisseria lactamica 2118이 생성하는 $\beta$-Galactosidase의 정제 및 성질

이종수,곽인영,김나미,Lee, Jong-Soo,Kwak, In-Young,Kim, Na-Mi 배재대학교 자연과학연구소 1991 自然科學論文集 Vol.4 No.-

p-Aminopheny1-$\beta$-D-thiogalactopyranoside agarose 친화성 크로마토그라피를 이용하여 Neisseria lactamica 2118이 생성하는 $\beta$-galactosidase를 정제한 후 몇가지 효소학적 성질을 조사 하였다. Neisseria lactamica 2118이 생성하는 $\beta$-galactosidase는 구성효소로서 lactose와 IPTG에 의하여 유도되지 않았다. 정제효소의 작용최적온도는 $35^{\circ}C$, pH는 7.5이었고 $50^{\circ}C$로 15~60분 처리시 약 80%의 활성이 유지되었으며 pH 6.0~9.0에서 안정 하였다. 또한 $Hg^(2+)$와 $Co^(2+)$에 의하여 그 활성이 저해 되었다. $\beta$-Galactosidase(EC 3.2.1.23) from pathogenic Neisseria lactamica 2118 was purified by p-aminopheny1-$\beta$-D-thiogalactopyranoside agarose affinity chromatography. Then some properties of the purified $\beta$-galactosidase were investigated.$\beta$-Galactosidase form Neisseria lactamica 2118 was constitutive enzyme, not induced by lactose and IPTG. Optimal activity was observed at $35^{\circ}C$ and pH 7.5 and the enzyme was stable at the range of pH 6.0-9.0 and at temperature below $50^{\circ}C$. The enzyme activity was inhibited by cations such as $Hg^(2+)$ and <Tex>$Co^(2+)$.

무형문화유산 Storytelling Marketing 연구 - 종로 '피맛골' 이야기를 중심으로 -

이종수,Lee, Jong soo 국립문화재연구원 2011 헤리티지:역사와 과학 Vol.44 No.1

본 연구는 우리나라의 지역전통 문화유산 마케팅을 위하여 문화유산에 전래적 이야기를 입혀 접근하는 감성적 마케팅인 스토리텔링 마케팅 방안을 접목하기 위한 연구이다. 즉, 본 고는 지역의 전통적 문화유산인 지역전통문화유산 특성(DNA)화와 마케팅을 위하여 지역문화유산의 하나인 피맛골 음식에 이야기를 입히는 스토리텔링 적용 방안과 체험사례 개발을 통한 마케팅 방안을 제시한다. 이를 위하여 문화재의 의의와 가치 등과 지역문화유산이란 무엇이며, 문화유산과 스토리텔링의 관계, 스토리텔링 마케팅 사례 분석 및 스토리텔링 적용방안과 체험거리 소재 등을 개발하였다. 구체적으로는 지역문화유산의 사례, 성공요인과 전략, 외국의 사례 등을 제시하였다. 문화유산이란 가장 좋은 자산이라기보다 오로지 유일한 자산이기 때문에 지역의 정체성과 진정성을 담아 국내,외에 알릴 수 있는 요긴한 자산이다. 주요 대상으로 한국의 문화 DNA중 하나인 '꾼, 장이, 뱅이' 등의 사례를 토대로 피맛골 DNA를 추출, 일부 벼슬아치와 보부상(褓負商), 일반 백성인 춘보, 옥섬 이야기 등을 예시하였다. 분석의 초점과 대상은 먼저 피맛골의 시공(時空)과 인간을 소재로 그의 역사성과 이미지, 특성과 유래 등을 재조명하고, 둘째, 스토리와 체험거리로 벼슬아치, 보부상 사례와 '춘보, 옥섬' 이야기를 설정하였으며, 셋째, 스토리 공유과정으로 '뱅이집'(보부상과 춘보 국밥집) 등을 체험할 수 있는 '피마촌' 건립을 제안하였다. The article is organized as follows. The first section clarifies research questions, the purpose of the study and the methodology used when researching cultural properties & storytelling marketing of intangible cultural heritages in Korea. The Pimatgol's DNAs are liberated areas of the nation, cooked rice served in soup, a broth to chase a hangover, makgeolli, so forth. The second section deals with methods of study, the literature review consisting of previous research, the author's previous research and the theoretical background of cultural heritage's storytelling marketing. The third section presents select storytelling marketing cases about our regional cultural heritage as well as some foreign cases. The fourth section provides a few examples and cases of cultural heritage about government officials, the 'Pimatgol' peddler, the story of Chunbo and Okseom and the idea for fostering storytelling marketing. The last section concluded the study. The findings support the importance of understanding the characteristics and differences of cultural heritage & storytelling marketing because if the stories are well told, the cultural heritages will be successfully promoted.

주거공동체에 대한 애착과 신뢰의 영향요인 분석 - 친밀권역(intimacy zone)의 회복을 위한 시각 -

이종수,Lee, Jong Soo 한국주거학회 2015 한국주거학회 논문집 Vol.26 No.1

Community is crucial for human beings not only because they are born with communal self but also because social cooperation is more efficient than competition in many cases. Human beings are defined as Homo communicus living in residential community which is important for the fulfillment of their primary needs. Residential community is an 'intimacy zone' where personal and emotional interactions are made. This study aims at examining the health of residential community in Korea by exploring the level of place attachment and neighborhood trust. Previous studies indicate that place attachment and trust are influenced by three dimensions such as residents' characteristics, house types and regional environment. This study conducted a nationwide questionnaire survey and it shows that place attachment moulds neighborhood trust. The results of path analysis demonstrates that place attachment and trust appear high in detached house areas. Age of residents also gives positive influence on the level of place attachment and neighbourhood trust. This result gives significant policy implications for the development of residential community in Korea. Residential community as an intimacy zone has been eroded and desperately needs to be revitalized. For this, more concern should be given to the issue of desirable size of residential community and the types of houses. Detached house area must be supported by governmental policies.