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이수재(Lee Su Jae) 한국사회체육학회 2003 한국사회체육학회지 Vol.19 No.1
It is a very important element for information producer to provide lots of internet information, which is changed, upgraded and erased in every second on the net, to information customer in a right way. A search and analysis of dance sports web sites for the revival and social activation leads not only cultivation and developments of domestic dance sports and also the renaissance of dance sports and more publicity of dance sports internationally. This research estimates how formation, presentation, and contents of every web sites which we can get from internet web engine, EOO company, works on the standard of Korean Standards Association(as of 2002). In details, the analysis focuses on whether having communication system for users such as information of web master, international language service, active image, optimum web browser, contents, main menu, site map, links with other sites, free board, guest book and so on. In result of this research, management, conservation, and repairs of present dance sports web sites should be ahead of creating a new web site. Also, absence of basic formation elements in many parts is a problem. Therefore, improvement of well-organized web sites for creative arts of dance sports more than definitional, historical, and technical information of dance sports is suppose to contribute dance sports to be a major international sports and scholastic position.
정석용(Suk-Yong Jung),이수출(Soo-Chool Lee),채호진(Ho-Jin Chae),이수재(Soo-Jae Lee),박정제(Jung-Je Park),안영수(Young-Soo Ahn),김홍수(Hong-Su Kim),김재창(Jae-Chang Kim) 한국청정기술학회 2008 청정기술 Vol.14 No.1
MCM 48, USY, beta 등과 같은 제올라이트를 세라믹종이에 실리카와 함께 도포하여 제습소자를 제조 하였고, H₂O 흡수 및 재생에 대한 평가를 고정층반응기에서 실시하였다. 제올라이트가 도포된 제습소자의 H₂O 흡수력은 제올라이트가 도포되지 않은 제습소자보다 약 1.5~2배 가량 높은 것을 볼 수 있었다. 특히 콜로이드 실리카와 MCM 48을 도포한 제습소자는 약 42.1g H₂O/g absorbent의 매우 우수한 제습 능력을 보여 주었다. 이러한 결과는 넓은 비표면적과 큰 기공부피를 가지고 있는 제올라이트에 의해 H₂O를 흡수할 수 있는 실리카 겔이 함침될 수 있는 공간이 증가하고 이에 따른 H₂O의 흡수량도 증가하는 것으로 판단된다. 또한 제올라이트가 도포된 제습소자는 80℃의 재생 온도에서도 쉽게 H₂O가 탈착되고, 이 때 탈착된 H₂O의 양은 제습소자가 흡수한 H₂O의 양과 동일하였다. 반복순환실험을 통한 H₂O의 흡수력 감소는 발견되지 않았고 초기 흡수력이 유지되었다. The dehumidifiers were prepared by the impregnation of the zeolites such as MCM 48, USY, beta on the silica ceramic paper. Their capacities for H₂O absorption and regeneration of the bed were tested in a fixed bed reactor system. The H₂O absorption capacities of the dehumidifiers impregnated with the zeolites were 1.5 ~ 2 times higher than those without the zeolites. Especially, the humidifier using MCM 48 and colloidal silica showed an excellent capacity (42.1 g H₂O/ g absorbent). It was found that the H₂O absorption capacities of the dehumidifiers were improved because the amount of silica gel, the main component in absorbing H₂O, increased due to the large surface area and pore volume of the zeolites. In addition, H₂O was easily desorbed from the dehumidifiers with zeolites at 80 of regeneration temperature and the desorption amount of H₂O was the same as that absorbed. It was confirmed that the H₂O absorption capacities of the dehumidifiers impregnated with zeolite were maintained without deactivation through the repeated cyclic experiments.
이수재,이정호,정해영,김규원 부산대학교 유전공학연구소 1993 분자생물학 연구보 Vol.9 No.-
효모의 질소대사의 조절 기작 중에시 Nitorgen Catabolite Repression(NCR)에 의해 그 발현이 조절되는 asparaginase Ⅱ의 구조 유전자인 ASP3 유전자을 대상으로 엽색체 서열을 비교한 결과, ASP3 유전자의 3' 인접염기서열이 rDNA 인접염기 서열과 동일하므로 염색체 DNA상에서도 ASP3 유전자가 rDNA에 인접하여 존재하는지를 규명하고자 한다. ASP3 유전자가 실제 염색체강에서 rRNA gene cluster에 삽입되어 있는지의 여부를 확인하기 위하여 plasmid YKW3를 다양한 제한효소로 절단하여 얻어진 여러 종류의 probe를 사용하여 Southern blot analysis를 한 결과 실제 효모 염색체상에 ASP3 유전자와 rRNA 유전자가 인접하여 있음을 확인하였다. 따라서 ASP3 유전자는 염색체 DNA에서 rDNA cluster중에 삽입되어 존재할 가능성이 높고, 또한 ASP3 유전자의 발현도 rDNA 유전자들의 발현과 관련성이 있음을 추정할 수 있다. Recently it was found that the 3' flanking sequences of the yeast ASP3 gene were the same as the flanking sequences of 5S rRNA gene. In this study, Southern blot analyses were carried out by using the plasmid YKW3 which contains the entire ASP3 gene to determine the localization of the ASP3 gene on the yeast chromosome. With various ASP3 gene probes, the identical bands were detected in both the chromosomal DNA and the YKW3 plasmid, For instance, when BglⅡ digested genomic DNA was hybridized with the 2.8kb BglⅡ fragment containing the region of homology with the 5S rRNA gene, the same 2.8kb band were identified. In addition, the same 1.8kb band were detected when PstⅠ digested genomic DNA was hybridized with the 2.8kb BglⅡfragment and 540bp EcoRⅠ- PstⅠ fragment, respectively. These results suggested that the ASP3 gene might lie adjacent or be inserted into the rRNA gene clusters on the chromosome.
Characterization of the asp Mutations in Asparaginase Ⅱ-deficient Yeast Strains
Kim, Kyu-Won,Hong, Young-Soo,Lee, Su-Jae,Chung, Hae-Young 부산대학교 유전공학연구소 1992 분자생물학 연구보 Vol.8 No.-
We have studied the genomic structure of the ASP3 gene in the wild type and asp mutant Saccharomyces cerevisiae strains. The asp2. asp3 and asp6 mutant strains did not synthesize asparaginase Ⅱ under both nitrogen-starved and nitrogen-rich conditions. Southern blot analysis showed that the ASP3 gene was completely deleted in the asp3 mutant strain. In contrast, the ASP3 gene structure was essentially intact in the asp2 and asp6 mutant strains. Northern blot analysis indicated that the asp2 and asp6 mutants did not synthesize ASP3 mRNA in spite of the presence of an intact ASP3 gene. These results implied that the loss of asparaginase Ⅱ was related to the extensive deletion of the structural gene in the asp3 mutant strain or probably some defects in the regulatory genes in the asp2 and asp6 mutant strains.