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        식물의 암종유발에 관한 연구 (제 3보) - Bacteria-free 암종조직의 획득에 있어서 항생제의 효과

        이민재,홍순우,최영길 한국미생물학회 1967 미생물학회지 Vol.5 No.2

        Up to the present time, there are only three methods by which we can obtain bacteria free crown gall tissue. According to some papers related to this field, the first method is based on the works of Braun(53') who maintained infected plants at 46-47'c for several days. But the method has a problem that very few plants can tolerate this temperature. The second method is based on the well known observation that old tumors appear to be bacteria free at least 1 or 2% of the explants. Also this method is known to us as laborious and time consuming. The third method is the one we were using that was attempting to kill the bacteria with bacteriacidal agent such as Antibiotics. In fact., it is reported that almost complete control of crown gall of tomato was obtained by Blanchard('51) when plants were grown in a nutrient containing Aureomycin(20${\mu}g$/ml) following needle puncture with the gall bacteria. We have been engaged in making the experiment by applying solution of Penicillin, Streptomycin and of Chloramphenicol(Succinate free) to find the strong bacteriacidal agent through the method of disc plate, and to confirm the effect of antimicrobial action through the method of plant tissue culture system without possible injury to the host plant. The result of this report is the fact the strongest bacteriacidal agent among the above three Antibiotics was Chloramphenicol(Succinate free 1000 p.p.m). and that there happened no injury to the tissue cultures in a White's 10X media containing 1000 p.p.m. of Chloramphenicol.

      • SCOPUSKCI등재

        식물의 암종유발에 관한 연구 2 (제 2 ) 에서 도마도 줄기에 유발된 의 Peroxidase Activitiy 에 대하여

        이민재,홍순우,최영길 한국미생물학회 1966 미생물학회지 Vol.4 No.2

        The relationships between tumor score and peroxidase activities of tomato stems infected with Agrobacterium tumefaciens strain A6Kl, B6, T372 11BNV6, 11BV7 and wounded stem as a control were examined in relation to crown gall tumor development on purpose to study the lignification of tumor tissue which is affected to the development of crown gall tumor. As the previous paper has been mentioned the fact that the induction of tumor tissues were inhibited or limited in the lignified stem of host plant. It was presumed that the activities of peroxidase related to the development of lignification were decreased during the period of tumor development. But the experimental result in this experiment shows that the peroxidase activities of crown gall tumor-tissues infected with the A. tumefaciens strains which are already known as virulent are increasing during four weeks, however, in the strain 11BNV6 and wound the peroxidase activities are decreasing on the second week after the inoculation of the bacteria strains. These results could be explained on the basis of that possible regulatory agents of lignification which were accumulated in tumor tissues, IAA, ascorbic acid, glutathion(GSH) and caffeic acid esters, were postulated to act as antioxidants which has been suggested by Stafford. Total nitrogen contents in relation to crown gall tumor development were determined for the detection of protein synthesis related to the enzyme activities which are increasing in the time of plant growth. Generally six groups are contained the largest amount of nitrogen on the second week after the inoculation of the bacterium. Comparing to the tumor score, it is presumed that the all of enzyme activities including peroxidase in tumor tissues are increasing from the second week through the third week after the inoculation of bacterium and the protein synthesis is stimulated under the most appropriated temperature during the above periods.

      • SCOPUSKCI등재

        식물의 암종유발에 관한 연구 1 (제 1 ) Agrobacterium tumefaciens 의 에 관하여

        이민재,홍순우,최영길 한국미생물학회 1966 미생물학회지 Vol.4 No.2

        As a part of studies of plant tumor induction, this experiment was prepared for the purpose of studying the ability of tumor induction and the tendency of tumor initiation in some Korean plants using the various Agrobacterium tumefaciens strains. Results obtained from this experiment are as follows. The virulences of five strains used in this experiment were gradually decreased in order of strain A6Kl, B6, 11BV7, T37 and 11 BNV6. Especially strain T37 which is known to the host limited strain showed virulent effect to the most of plants given for the materials as well as strain A6Kl, B 6 and 11BV7. Concerning the grade of tumor development, in plants which has tough stem, for example, Glycine max Meer, tumor induction was not well developed after the inoculation of all strains. Particullary in Ricinus communes Linne all strains showed virulent effect but tumor tissues were declined in relation to the development of lignification. It was also confirmed that the induction of tumor tissues on plants is to delay according to the increase of the age of host plants.

      • SCOPUSKCI등재

        Streptomyces속의 분리 및 동정에 관한 연구

        이민재,하영칠,안정선 한국미생물학회 1976 미생물학회지 Vol.14 No.1

        A taxonomical sutdy was made on the Streptomyces species isolated from soils in this country, most of which were collected during the period from April, 1974 to July 1975. J.S.P. Methods (1966), I.S.P. Descriptions (1968-1972) and Bergy's Manual of Determinative Bacteriology (1974) were used for most of the experimental methods and identifications. As a result, 24 species were identified as follows ; S. albolongus, S galticri.S. Nashvillensis. S. showdoensis. S.norbonensis. S. flacocirens. S. resistomycificus. S> reshiriensis. S. chromofuscus. S. parvullus. S, chibaensis. S.canus. S. albulus. S. amlachiticus. S.griseoflavus. S. griscoincarnotus. .S. rubiginosus. S. bacillaris. S. setonill. S.intermedius. S. griseinus. S.subrutilus. S.reseosporus.

      • KCI등재

        식민지 조선의 연료 이용 정미기 보급과 1920~1930년대 무연료 정미기 발명 ―사회적 맥락과 의미를 중심으로―

        이민재 국립민속박물관 2019 민속학연구 Vol.0 No.44

        This research focused on the dramatic change of rice mill, which is one of the cultural shift in agriculture and rural society. Especially, introduction and diffusion of a fuel-using rice-polishing machine and invention of non-fuel using rice-polishing machine which is reaction of diffusion of fuel-using rice mill machine is the main subject of this research. For this study, reporter, articles, patents, statistical data, and newspaper which are published in Colonial Korea period become subject of analysis. Through this research, introduction, and diffusion of fuel-using rice-polishing machine was not “a course of modernization” by technical progress. Instead, this change proceeded in commercialization toward Japan. In particular, Office of the Governor General of Chōsen carried out agricultural policies that also increase rice production, but the turnover increased even more. As a result, fuel-using rice-polishing machine became more popular. However, rural communities and farm households in the 1920s and 1930s of Colonial Korea period could not fully accept the fuel-using rice-polishing machine. because, through the 1920s and 1930s, rural communities and farming households in Colonial Korea experienced a very difficult economic situation. In this situation, the fuel-using rice-polishing machine, which required a lot of capital and fuel, was a burden on rural communities and farm household in Colonial Korea In this reality, the inventors invented the non-fuel rice-polishing machines as an alternative to the fuel-using rice-polishing machine. Inventors wanted to invent the non-fuel rice-polishing machines, which could install a lower initial cost than the fuel-using rice-polishing machine, could operate by manpower, axial force, and hydraulic power and that is more productive than traditional rice mills. As a result, the non-fuel rice-polishing machine was not widely available in Colonial Korea. However, the invention of the non-fuel rice-polishing machine in the 1920s and 1930s was the result of the new actors, the inventors of the Colonial Korea, who realized the critical consideration they saw from the perspective of Colonial Korea's rural society and farming households, not from the perspective of Capitalist and Imperial Japan. 이 연구는 급속도로 변한 도정도구 변화를 단순한 기술발전이 아니라 쌀의 상품화와 그에 따른 농업·농촌의 문화 변동이라고 여기고 이를 연구대상으로 삼았다. 특히 19세기 말 시작된 연료 이용 정미 도입과 식민지시기를 전후로 보급되는 과정 그리고 이에 대한 반작용(reaction)이라 할 수 있는 1920~1930년대 무연료 정미기 발명과 발명가들에 대해서 살펴보고자 한다. 이를 위해 식민지시기 출간된 조선미(朝鮮米)와 정미기에 대한 연구서, 특허문서 및 통계자료 및 신문 기사 등을 1차 사료로 삼아 분석하였다. 이 연구를 통해 연료 이용 정미기 도입과 보급은 기술 발전에 따른 ‘근대화’ 과정이 아니라 조선미(朝鮮米)가 1890년을 기점으로 일본을 소비시장으로 하는 상품화 과정 속에서 이뤄졌음을 확인할 수 있었다. 특히 조선총독부가 실시한 산미증식계획을 바탕으로 하는 생산량 증가와 이출량 증가 및 미곡검사제도는 연료 이용 정미기 보급을 더욱 추동한 원인이 되었다. 하지만 1920~1930년 식민지 조선의 농촌사회와 농가, 특히 영세농가들은 연료 이용 정미기를 완전히 받아들일 수 없었다. 왜냐하면 1920년대 전반기 조선총독부의 식민지 농정과 농업의 일본 시장 종속화로 인하여 양극화를 격심하게 겪었고 1920년대 후반부터 세계대공황으로 촉발된 농업공황을 거치면서 하위에 속하는 많은 농가가 몰락한 상황이었다. 이러한 상황에서 설비와 운용에 많은 자본이 필요한 연료 이용 정미기는 식민지 조선의 농촌사회와 농가에 부담이었다. 무연료 정미기 발명가들은 이 같은 현실 속에서 연료 이용 정미기의 대안으로 무연료 정미기를 발명했다. 발명가들은 연료 이용 정미기보다 초기비용이 저렴하고 석유가 아닌 인력, 축력, 수력 등을 동력원으로 운용하며 더 나아가 재래 도정도구보다 뛰어난 생산성을 가질 수 있는 무연료 정미기를 발명하고자 했다. 결과적으로 무연료 정미기는 식민지 조선에 폭 넓게 보급되지는 않았다. 그러나 1920~1930년대 무연료 정미기 발명은 식민지 조선의 발명가라는 새로운 행위자들이 연료 이용 정미기 보급이라는 ‘근대화’에 대해 자본과 제국 일본의 시선이 아니라 식민지 조선 농촌사회와 농가의 시선에서 바라본 비판적 고찰을 현실화한 결과물이라 할 수 있다.

      • KCI등재

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