담낭절제술시 경담낭관 조영술의 의의

이명복(Myung Bog Lee),이민혁(Min Hyuk Lee),허경열(Kyung Yul Hur),송영식(Young Sik Song),허경발(Kyung Bal Hur) 대한소화기학회 1994 대한소화기학회지 Vol.26 No.6

N/A The routine use of operative cystic duct cholangiography(OCDC) has been advocated by nu- merous authors. In these studies, the value of OCDC in the detection of unsuspected small stones of the common bile duct was demonstrated. The procedure also appears to be of value in reducing unnecessary exploration of the common bile duct in patients otherwise having clin- ical indications for such exploration. But opinion is divided as to whether cholangiography should be performed in all cholecystectomies or only in selected cases. To determine the clinical usefulness of routine OCDC, we analyzed 307 patients undergoing cholecystectomy with routine OCDC for gallstone disease were retrospectively screened for the presence of 8 parameters of possible choledocholithiasis. Of the 307 patients studied, 241 pa- tients(78.5%) had a negative OCDC(group I ), 22(7.1%) had false positive findings (group II), two(0.7%) had false negative findings(group III), and 42(13.7%) had findings positive for stones(group IV). In group I, 81 pat.ients(33.6%) had one or more parameter of possible choledocholithiasis. In group II, 16 patients(72.7% ) had one or more parameter. In group III, 2 patients(100%) had one or more parameter. In group IV, 5 patients(11.9%) without any positive parameter had choledocholithiasis. The sensitivity of OCDC was 95.5%, specificity 91. 4%, efficiency 92.2%, predictability of a negative test 99.2%, predictability of a positive test 65.5%, and accuracy 92.8%. Routine OCDC showed unsuspected common bile duct stones in 5 patients(2.9%). Unnecessary common bile duct explorations were avoided in 81 of the 136 pa- tients(59.5%). As a result of these findings, we recommend the use of OCDC routinely in pa- tients undergoing cholecystectomy. (Korean J Gastroenterol 1994; 26: 952 961)

14세 여아에서 발견된 선천성 낭종성 선종성 기형

이명인 ( Myung In Lee ),손소희 ( So Hee Sohn ),이대준 ( Dae Joon Lee ),하동열 ( Dong Yul Ha ),지영구 ( Young Koo Jee ),이계영 ( Kye Young Lee ),김건열 ( Keun Youl Kim ),최영희 ( Young Hi Choi ),조정희 ( Jeong Hi Cho ),서필원 ( Pil 대한결핵 및 호흡기학회 1996 Tuberculosis and Respiratory Diseases Vol.43 No.5

미나리 抽出物이 家兎 肝腸機能에 미치는 影響

서화중(Hwa-Joong Seo),이명열(Myung-Yul Lee) 한국식품영양과학회 1985 한국식품영양과학회지 Vol.14 No.1

This studies were conducted in an attempt to investigate the effect of Oenanthe javanica extract on enzyme activities of liver and excretory action of bile juice in serum of carbon tetrachloride-intoxicated rabbits. Long-term oral administration of this extracts shows more rapid recuperation in GPT, total cholesterol, alkaline phosphatase, total bilirubin and BSP values than control groups. 750 ㎎/㎏-administered group shortens rapidly the recovery time of GPT, total cholesterol and BSP values than 250 ㎎/㎏ administered groups. But any difference was not observed in alkaline phosphatase, bilirubin among two groups. In histological studies the lager amount-administered groups exhibited more rapid recovery of liver cell enlargement, oedema, necrosis in carbon-tetrachloride treated rabbits.

益母草綠汁이 家兎의 肝臟障碍 및 高血糖에 미치는 影響

서화중(Hwa-joong Sheo),국현숙(Hyun-sook Kook),이명열(Myung-yul Lee) 한국식품영양과학회 1987 한국식품영양과학회지 Vol.16 No.1

These studies were conducted to investigate the effects of Leonurus sibiricus extract (L.S.E.) on experimentally CCI₄-induced liver damage and alloxan-induced diabetes in rabbits;<br/> Leonurus sibiricus juice (L.S.J.) showed the most rapid recuperation of liver function among samples compared to the control group in CCI₄-intoxicated rabbits. SGPT activity showed an apparant decreasing effect in 4 days, and the blood level of total cholesterol and total bilirubin in 6 days, and not in alkaline phosphatase level.<br/> L.S.J. exhibited more excellent hypoglycemic effect in alloxan-induced diabetes of rabbits, that is, significant to the control group after 2 days and adjacent to the normal level on 8th day. And SGPT activity and total cholesterol level were gradually decreased and showed markedly decreasing effect after 4 days.

枸杞子 抽出物이 家兎의 實驗的 肝臟障碍 및 alloxan糖尿病에 미치는 影響

서화중(Hwa-Joong Sheo),전성주(Sung-Joo Jun),이명열(Myung-Yul Lee) 한국식품영양과학회 1986 한국식품영양과학회지 Vol.15 No.2

枸杞子抽出物을 試料로 mouse의 毒性實驗, 實驗的으로 誘發시킨 家兎의 肝臟機能障碍 및 alloxan負荷糖尿病에 미치는 影響을 實驗하여 다음과 같은 結果를 얻었다.<br/> 1. Mouse의 急性毒性實驗에서 腹腔投與의 경우 平均致死量은 12.7g/㎏이었다.<br/> 2. CCl₄로 肝臟機能障碍가 誘發된 家兎에 枸杞子抽出物投與는 對照群에 비하여 有意性있는 回復度를 보였는데 特히 大量投與群에서 더욱 빨랐다.<br/> 1) GPT活性度 및 total bilirubin은 試料投與群에서 아주 優秀한 低下效果를 나타냈다. 特히 GPT活性度에서 200㎎/㎏은 第8日째부터, 800㎎/㎏은 第6日째부터 有意性이 있었다.<br/> 2) Alkaline phosphatase値는 對照群에 비하여 커다란 차이를 보이지 않았다.<br/> 3) Alloxan으로 誘發한 家兎의 高血糖에 대하여 800㎎/㎏는 아주 탁월한 血糖降下效果를 나타냈다. 卽 第4日째부터 對照群에 비하여 有意性이 있었으며 第12日째는 正常値에 거의 接近하였다. 또한 GPT活性度는 차츰 低下되어 第6日째부터 低下效果가 뚜렷하였다. These studies were carried out to investigate effects of Lycii fructus extract on experimentally CCl4-induced liver damage and alloxan-induced diabetes in rabbits and acute toxicity using mice.<br/> LD_(50)(㎎/㎏) of Lycii fructus extract(L.F.E.) was 12.17g/㎏ by intraperitoneal administration in mice.<br/> L.F.E. showed more rapid recuperation compared to the control group in CCl4-intoxicated rabbits and 800㎎/㎏ was the most effective.<br/> Especially GPT activity and total bilirubin level showed an apparant decreasing effect within 6 days and 8 days, respectively in 800㎎/㎏.<br/> But any differences were not observed in alkaline phosphatase and total cholesterol between sample and control group.<br/> Large amount administered group exhibited more excellent hypoglycemic effect in alloxan-induced diabetes of rabbits, that is, it was significant to the control group after 4 days and adjacent to the normal level on 12th day. And GPT activity was gradually decreased and showed clear decreasing effect after 6 days.<br/> It is suggested that L.F.E. can be administered not only as therapeutic agents (such as liver tonics or antidiabetetics) but also a natural food to shorten the recovery time of hepatic function in liver diseases and decrease the abnormally elevated blood glucose such as Diabetes Mellitus.

梅實抽出物이 家兎의 Alloxan 糖尿病에 미치는 影響

서화중(Sheo Hwa Joong),고은영(Ko Eun Young),이명열(Lee Myung Yul) 한국식품영양과학회 1987 한국식품영양과학회지 Vol.16 No.3

These studies were conducted to investigate effects of Prunns mume extract on experimentally alloxan-induced diabetes in rabbites, antibacterial activity and acute toxicity in mice;<br/> 1. LD_(50) of Prunus mume extract(P. M. E.) was 15.25g/㎏ intraperitoneally in mice.<br/> 2. P. M. E. showed more significant recuperative effect compared to the control group in alloxan-induced diabetes of rabbits.<br/> 1) P.M.E. 800㎎/㎏ bodywelght exhibited more excellent, hypoglycemic effect afte 6 days and adjacent to the normal level at 14th day.<br/> 2) SGPT activity was significantly decreased after 6 days, and the blood levels of total cholesterol and urea-nitrogen were significant in 800㎎/㎏ at 6 days and 10 days respectively.<br/> 3. In antibacterial activity test P.M.E. was active at 0.195㎎/㎖ in Staphyllococcus aureus, 3.125㎎/㎖ in E. coli, Enterobacter cloacae, Klebsiella oxytoca, and in Pseudomonas aeruginosa at 0.391㎎/㎖.<br/> 4. In histological findings, the sample groups were deeply stained, fully granulated and partial degranulation of β-cells, and a few vacuolar and vesicular changes of cytoplasm than alloxan treated group in proportion to the sample amounts.

珍島産 枸杞子의 脂肪酸組成에 關한 硏究

李明烈,申惠盛,徐華中 조선대학교 기초과학연구소 1986 自然科學硏究 Vol.9 No.1

These systematic analyses were carried out in order to investigate approximate compositions, and the isolation and identification of fatty acid in Lycii fructus (L.F.) by thin layer chromatography and gas chromatography. 1. L.F. contained 22.00% crude proteins, 12.19% crude lipids and had 134 iodine value. 2. L.F. contained 12.19% total lipids, of which free and bound forms were 11.40% and 0.79% respectively. Free forms were mosty consisted of neutral lipids (97.80%), and in bound forms 58.20% neutral lipids, 15.50% glycolipids and 26.30% phospholipids. 3. Triglyceride was the most abundant component, and ES, FFA, FS, DG and MG were fractionated and identified in neutral lipids of the free form, and ES, TG, FFA, FS and DG in the bound form. 4. MGDG was contained more than 5 times in glycolipids of bound form and no difference were observed between both forms in phospholipids. 5. The highest content of fatty acids was linoleic acid (C_18=2) In free and bound forms, and oleic acid (C_18=1), palmitic acid (C_16=0) and linolenic acid (C_18=3) were found in abundance.

광주 근교산 붕어의 아미노산 및 지질성분의 분석

李蓮玉,朴貞淑,金忠模,李炳燮,李明烈 조선대학교 기초과학연구소 1992 自然科學硏究 Vol.15 No.1

광주 근교산 붕어의 일반성분, total amino acid 및 free amino arid를 HPLC로 분석 그리고 유리지질과 결합지질을 추출하고 이를 각각 column chromatography에 의하여 중성지질, 당지질 및 인지질로 분획한 다음 이를 각 지질의 구성 지질을 TLC scanner, GC 등으로 분리 정량한 결과는 다음과 같다. 1. 붕어의 조단백질은 5.6%, 조지방은 5.4% 이었다. 2. 총 아미노산 및 유리아미노산은 17종이였으며, 총아미노산은 glycine이 많고 유리아미노산은 arginine이 많이 함유되어 있으며, 필수아미노산량은 총아미노산량의 약 28%를 차지하였다. 3. 총지질 중 유리지질이 5.2% 및 결합지질이 0.2%가 함유 되었으며 유리지질을 구성하는 지질의 함량을 중성지질 81.2%, 인지질 25.5%, 당지질 1.3%로 구성 되였으며 결합지질에는 중성지질 25.5, 인지질 70.0%, 당지길 4.5%로 구성되었다. 4. 유리 및 결합지길 중의 중성지질로는 monoglyceride, diglyceride, free sterol, fatly acid, triglyceride, esterified sterol 등이 분리 동정 되었으며 이중 triglyceride가 유리지질에 82.8%결합지질에 46.1%로 가장 많이 함유되었다. 5. 유리지질과 결합지질을 구성하는 인지질로는 phosphatidyl choline, lecithin, phosphatidylglycerol, phosphatidyl serine, steryl glycoside+phosphatidyl ethanolamine 등이 분리동정 되었으며 유리지질의 경우 phosphatidy1 choline이 2.8%, 결합지질에는 lecithin이 43.5%가 함유되었다. 6. 총지질을 구성하는 주된 지방산은 C_16, _OC18 1의 함량이 가장 많았으며 중성지질은 C_18 2의 함량이 높았으며, 당지질은 유리지질이 C_14 1, C_20 1이 다량 함유되어 있으며 결합지질은 C_20 4,C_18 1이 다량 함유되어 있다. 인지질의 지방산 조성은 C^19_0이 다량 함유되어 있으며 특히 결합지질에는 C_22 6이 다량 함유되어 있다. Approximate compositions, total amino acids and free amino acid, and the isolation and identification of fatty acids in Carassius auratus were analyzed by high performance liquid chromatography, thin layer chromatography and gas chromatography. The results are; Carassius auratus contained 73.8% water, 5.6% crude protein and 5.4% crude fat. 17 kinds of total and free amino acids were analyzed, respectively, glycin is the richest in total amino acids and arginine in free amino acids and total amounts of the essential amino acid were about 28% of total amino acids. Total lipid was contained 5.2% free lipid and 0.2% bound lipid. Free forms and bound forms were mostly consisted of neutral lipids(81.2%) and phospholipid(70.0%), respectively.

ROSA RUGOSA 根이 家兎의 實驗的 高血糖 및 高脂肪血症에 미치는 影響

李明烈,丁明鉉 조선대학교 약학연구소 1984 藥學硏究誌 Vol.6 No.1

The influence of extracts and crude saponin of Rosae rugosae Radix on experimentally induced hyperglycemia and hyperlipidemia in rabbits, acute toxicity using mice, saponin certification were investigated. Rosae Rugosae radix showed significant hypoglycemic effect and decreased the peak glucose level and shortened the recovery time. And it reduced high concentrations of total serum cholesterol and triglyceride levels significantly. It was considered that Rosae rugosae Radix can be administered as an effective hypoglycemic and hypolipidemic agent.

光州 近郊産 淡水魚의 脂質成分의 分析 : 잉어 및 가물치를 중심으로 Cyprinus carpio and Channa argus

李炳燮,朴貞淑,李明烈 조선대학교 기초과학연구소 1992 自然科學硏究 Vol.15 No.1

淡水魚인 가물치 및 잉어를 試料로 一般成分 分析과 遊離脂質과 結合脂質을 抽出하고 이를 각각 column chromatography에 의하여 中性脂質, 糖脂質 및 燐脂質로 분획한 다음 이들 各 脂質의 構成脂質을 TLC scanner, GC 등으로 分離, 定量한 結果는 다음과 같다. 1. 總脂質은 가물치 2.9% 및 잉어 4.4% 이었으며 가물치에는 遂離脂質이 2.6%, 結合脂質이 0.3%, 잉어에는 遊離脂質이 4.3%, 結合脂質이 0.1%가 含有되었다. 2. 遊離脂質을 構成하는 脂質의 含量은 中性脂質의 경우 가물치 96.3%, 잉어 95.8% 이었으며 糖脂質은 가물치 2.8%, 잉어 1.7%이고 燐脂質은 가물치 0.9%, 잉어 35.7%가 含有되어 있으며 結合脂質에는 中性脂質이 가물치 24.2%, 잉어 35.7%이고 糖脂質은 가물치 2.2%, 잉어6.9% 燐脂質은 가물치 7.36%, 잉어 57.4%로 各 試料에서 遊離脂質 중에는 中性脂質의 含量이 높았고 結合脂質에는 燐脂質의 含量이 높았다. 3. 遊離 및 結合脂質 중의 中性脂質로는 monoglyceride, diglyceride free sterol, fattyacid, triglyceride, esterified sterol 등이 分離 同定되었으며 이 중 trig1yceride가 遊離脂質에82.8~95.3%, 結合脂質에 35.I~43.3%로 가장 많이 含有되었다. 4. 遊離脂質과 結合脂質을 構成하는 燐脂質로는 phosphatidyl choline, leacithin, phoshatidyl glycerol, phosphatidyl serine, steryl glycoside+phosphatidyl ethanolamine 등이 分離同定 되었으며 遊離脂質의 경우 各 試料에서 phosphatidyl choline이 30.8~42.6%, 結合脂質에는 leacithin이 31.8~32.0%가 含有되었다. 6. 總脂質을 構成하는 주된 脂肪酸은 各 群에서 C_16.0C_1^8_1의 含量이 가장 많았으며 中性脂質에는 各 群에서 C_l8 1, C_18 2, 糖脂質에는 遊離脂質이 가물치가 C_20 1, 잉어가 C_l8 1로 多量含有되어 있으며 結合脂質은 가물치가 C_18 1, 잉어 C_16 1이 含有되어 있다. 燐脂質의 脂肪酸조성은 遊離脂質은 各 群에서 C_18 o, 結合脂質은 料 群에서 C_18 0이 多量 含有되어 있었다. These systemic analyses were carried out to investigate approximate compositions, and the isolation and identification of fatty acid in fresh-water fishes(cyprinus carpio and Channa argus) by thin layer chromatography and gas chromatography. The results were follows ; Total lipids were contained 2.9% in Channa argus and 4.4% of Cyprinus carpio, and Channa argus had 2.6% free lipid and 0.3% bound lipid and 4.3% free lipid and 0.1% bound lipid in Cyprinus carpio, respectively. Free forms were mostly consisted of neutrall lipids(95.8∼96.3%) and in bound forms 57.4∼73.6% phospholipids in each sample. TG(Triglyceride) was the most abundant component, MG(Monoglyceride), DG(diglyceride), FS(free sterol), FFA(free fatty acid) and ES(Esterified sterol) were fractionated and identified in neutral lipids of the free and bound lipid in each sample. The glycolipids contained in free and bound lipids were identified as 7 components, of which MGDG(monogalactosyl diglyceride) was most abundant in free lipids and DGDG(digalactosyl diglyceride) in bound lipids in each sample. The phospholipids in free and bound lipids were identified as 5 components, of which the highest content was PC(phosphatidyl choline) in free lipid and LT(lecithin) in bound lipid.