MAPKs의 활성화 기전을 나타내는 항염증 소재의 탐색

이경호,이기형,Lee, Keyong Ho,Rhee, Ki-Hyeong 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.3

Lonicera japonica 에탄올 추출물의 처리에 의하여 LPS에 의해서 활성화된 RAW 264.7 세포에서 NO의 생성량과 TNF-${\alpha}$, IL-$1{\beta}$ 및 IL-6와 같은 염증성 사이토카인의 분비를 억제하였고, MAPK family인 ERK, p38 및 JNK의 인산화와 $I{\kappa}-B{\alpha}$의 분해를 억제하였다. LPS로 유도한 endotoxin shock 동물실험에서 Lonicera japonica 에탄올 추출물 20 mg/kg에서 LPS로 유도한 endotoxin shock에 대한 생존율을 3배 이상 증가시켰으며, 생존시간도 1.3~1.4배 증가시켰다. The aim of this study was to evaluate the potential anti-inflammorty properties of herbs via MAPKs such as ERK, p38, JNK. Fifty-one kinds of each herbal medicine, that were extracted with ethanol, were used in the inhibitory assay of cytokine (TNF-${\alpha}$, IL-$1{\beta}$ and IL-6) and NO. of these, 10 species of herbal medicines, Angelica dahurica, Atractylodes lancea, Cnidium officinale, Duchesnea chrysantha, Oldenlandia diffusa, Lonicera japonica, Paeonia lactiflora, Pinus thunbergii, Rehmannia glutinosa and Rubus coreanus, were screened as potential inhibitors (< $300{\mu}g/mL$) of NO, TNF-${\alpha}$, IL-$1{\beta}$ and IL-6. Among the 10 species, Lonicera japonica showed potential anti-inflammatory effects Lonicera japonica extract of $200{\mu}g/mL$ inhibited the phosphorylation of ERK, p38 and JNK. In addition, Lonicera japonica extract at 20 mg/kg increased survival rate from LPS-induced endotoxin shock by 3 fold.

후코이단에 의한 니코틴의 코티닌 전환 효과

이경호,이기형,Lee, Keyong Ho,Rhee, Ki-Hyeong 한국식품영양학회 2014 韓國食品營養學會誌 Vol.27 No.4

본 연구에서는 nicotine 분해에 효과에 대하여 해양식물 유래의 후코이단을 이용하여 시험관에서 직접 혼합법 및 세포주 시험에서 nicotine의 cotinine으로의 전환능을 측정하였다. 직접 혼합법 시험결과, 후코이단 $1{\mu}g/mL$에서 시간이 경과함에 따라서 nicotine의 cotinine으로의 전환 정도가 대조군 대비 증가하여 시험 종료 시점에서 15배의 증가율을 나타내었다. 세포주을 이용한 nicotine의 분해능 시험 결과, 시험 종료 시점에서 대조군 대비 6배의 cotinine 증가율을 나타내었다. 양성 대조군으로 사용한 녹차 추출물 대비 nicotine 분해능은 우수한 것으로 평가되었다. 그러나 후코이단의 항산화능은 녹차 추출물과 대비하여 낮았으며, 항산화 주요 기능을 갖는 폴리페놀 및 플라보노이드 성분 역시 낮았다. 본 시험 결과가 나타내는 바는 후코이단의 nicotine의 cotinine으로의 전환능에 대한 입증을 하였으며 이는 금연 보조 역할을 할 수 있는 기능성 소재로 사료된다. This study aims to find the correlation the low-molecule fucoidan for cancer prevention with an accelerated formation of cotinine. In the presence of fucoidan, a nicotine to cotinine coversion was studied in established assay, direct mixture method and Hep-G2 cell line method. Fucoidan of $1{\mu}g/mL$ showed the potential effect for converting nicotine to cotinine in the direct mixture method compared to control. Increase of conversion rate at the treatment of fucoidan is exhibited as 15 times compared to control. In Hep-G2 cell treatment, fucoidan showed the potential activity for converting nicotine to cotinine as 6 times compared to control. Therefore, fucoidan was shown to be effective in the conversion of nicotine into cotinine even though it is not higher content of polyphenol and flavonoid than its of green tea extract.

감초 추출물의 Dextran Sulfate Sodium 유도 마우스 궤양성 대장염 억제효과

이경호 ( Keyong Ho Lee ),이기형 ( Ki Hyeong Rhee ) 한국식품영양학회 2010 韓國食品營養學會誌 Vol.23 No.4

The aim of this study was to evaluate the anti-inlfammatory effects of glycyrrhiza glabra Linne extract on ulcerative colitis induced by 3% dextran sulfate sodium in mice. The experimental animals were divided into six groups: control(normal), DSS-induced colitis(control), 1㎎/㎏, 10㎎/㎏, and 100㎎/㎏ of glycyrrhiza glabra Linne extract, and 150㎎/㎏5-aminosalicylic acid(5-ASA)(positive control). We evaluated the pathological disease activity index(DAI), change in weight, colon mucosa damage and myeloperoxidase(MPO) in colon mucosa. Treatment with 10㎎/㎏ and 100㎎/㎏ of glycyrrhiza glabra Linne extract led to significant loss of body weight, the decrease of MPO activity and clinical symptoms such as DAI and histological change. In particular, 100㎎/㎏ Glycyrrhiza glabra Linne extract led to markedlygreater improvement than 150㎎/㎏ 5-ASA treatment. These results suggest that glycyrrhiza glabra mediated anti-inflammatory action on colorectal sites may be a useful therapeutic approach to ulcerative colitis.

Hollow Fiber Assay을 이용한 고량강 추출물의 항종양 효과

이경호 ( Keyong Ho Lee ),이기형 ( Ki Hyeong Rhee ) 한국식품영양학회 2011 韓國食品營養學會誌 Vol.24 No.4

The purpose of this investigation was to evaluate anti-tumor activity and detect what compounds affect its activity form Alpinia officinarum Hance. Two fractions, methanol and ethylacetate, were isolated by Amberlite XAD-2 resin column chromatography from methanol extract of the rhizomes of Alpinia officinarum. In hollow fiber assay, the methanol extract and methanol fraction were found to inhibit the tumor growth against colon tumor cell lines such as Colo-320, HCT116 and WiDr. Three diarylheptanoids [5-hydroxy-1,7-diphenyl-3-heptanone, 5-hydroxy-7-(4``-hydroxy-3``-methoxyphenyl)-1-phenyl- 3-heptanone and 3,5-dihydroxy-1,7-diphenylheptane] and two flavonoids [galangin and kaempheride] were isolated and identified from the methanol fraction, which is higher activity than ethylacetate fraction. Among these diarylheptanoids and flavonoids, 3,5-dihydroxy-1,7-diphenylheptane, galangin and kaempheride as active components on anti-tumor activity were mainly posited in methanol fraction.

향나무 추출물의 Galectin-3 저해에 의한 암 전이 억제 효과

이경호 ( Keyong Ho Lee ),이기형 ( Ki Hyeong Rhee ) 한국식품영양학회 2012 韓國食品營養學會誌 Vol.25 No.4

루테인과 후코이단 병용 처리에 의한 AAPH 유도 세포 손상 억제

이경호 ( Keyong Ho Lee ),윤원호 ( Won Ho Yoon ) 한국식품영양학회 2010 韓國食品營養學會誌 Vol.23 No.3

This study was designed to investigate the protective effect of the combination of fucoidan and lutein against AAPH-induced oxidative stress in THP-1 cells. The combination of fucoidan and lutein existed significant antioxidant effect on AAPH-damaged THP-1 cells by using lipid peroxidation and cellular antioxidant capacity assay. Fucoidan(1㎍/㎖) and lutein(10㎍/㎖) did not affect at all the viability of THP-1 cells, but protected the AAPH-damage of THP-1 cells at the same concentration. The viability of THP-1 cells was 0% with 1 mM AAPH alone, the protective effect of fucoidan(1㎍/㎖) and lutein(10㎍/㎖) was 37% and 36%, respectively. The combination of fucoidan(1㎍/㎖) and lutein(10㎍/㎖) exhibited significant inhibitory effect of lipid peroxidation using TBARS assay and cellular antioxidant capacity using DCFH-DA assay. In lipid peroxidation, the TBARS value of 1mM AAPH alone was 0.8±0.03nM MDA, its of the combination of fucoidan(1㎍/㎖) and lutein(10㎍/㎖) was 0.2±0.05nM MDA. In cellular antioxidant capacity, the combination of fucoidan(1㎍/㎖) and lutein(10㎍/㎖) exhibited significant cellular antioxidant capacity of 76%, whereas quercetin(10μM) as positive control exhibited the cellular antioxidant capacity of 32%. These results indicate that the cotreatment of fucoidan and lutein protects against AAPH-induced THP-1 cell damage by inhibiting lipid peroxidation, increasing cellular antioxidant capacity.

Clonogenic Assay를 이용한 Aloe arborescens와 Aloe vera 용매 추출물의 종양세포 억제효과의 비교

홍희선,이경호,김정환,강희곤,조좌형,김창한,Hong, Hee-Sun,Lee, Keyong-Ho,Kim, Jeong-Hwan,Kang, Hee-Gon,Cho, Choa-Hyoung,Kim, Chang-Han 한국생약학회 1999 생약학회지 Vol.30 No.3

The solvent extracts from Aloe arborescens and Aloe vera were randomly screened for inhibitory effects on the growth of tumor cell lines. In case of Aloe vera extracts, butyl alcohol extract and ethyl alcohol extract showed antitumor activity at $100\;{\mu}g/ml$ on lung cell lines(A427, Sk-mes-1, Calu-3 and 3LL). In Aloe arborescens extracts, butyl alcohol extract and ethyl alcohol extract exerted high activity at $100\;{\mu}g/ml$ on breast cell line(Hs-578T) and lung cell line(Sk-mes-1), respectively. The solvent extracts from Aloe vera exerted antitumor activity broadly on various tumor cell lines. In contract, the solvent extracts from Aloe arborescens exerted specific antitumoricity on a few tumor cell lines.

차나무 종자 추출물의 항균활성 및 항종양활성

윤원호,최재훈,이경호,김창한,Yoon, Won-Ho,Choi, Jae-Hoon,Lee, Keyong-Ho,Kim, Chang-Han 한국식품과학회 2005 한국식품과학회지 Vol.37 No.1

본 연구는 폐기되는 차나무 열매를 이용하여 향균활성과 항종양활성을 비교하고 이용가능성에 대하여 탐색하고자 하였다. 항균활성과 항종양활성을 알아보기 위하여 70% 에탄올과 물 추출물의 활성을 측정하였다. 항균활성은 식중독균과 몇 종의 병원균, 효모, 곰팡이 등 17균주에 대하여 추정하였고, 항종양 활성은 6종류의 인체 암세포주, 1종류의 정상세포주에 대하여 조사하였다. 차나무 잎, 종자와 과피의 항균활성을 비교한 결과는 종자의 70% 에탄올과 물 추출물에서 효모에 대하여 항균활성이 높게 나타났다. 특히, candida albicans, Cryptococcus neoformans에 대하여 항균활성을 보였다. 종자 물 추출물을 $80,\;100^{\circ}C$에서 30분, $121^{\circ}C$에서 15분간 열처리 후 항균활성을 검색한 결과 가열온도에 따른 차이는 크게 나타나지 않았다. 항종양 활성을 검색하기 위하여 MTI assay를 통해 활성을 측정하였다. 과피 추출물에서 전혀 활성이 없었지만 종자 추출물에서는 큰 활성을 나타내었다. 극성용매인 70% 에탄올과 물 추출물의 종양세포 증식억제효과가 높게 나타났으며 그중에서 물 추출물이 70% 에탄올 추출물 보다 높게 항종양 활성이 나타났다. 차나무 종자로부터 추출한 활성물질의 종양세포와 정상 세포에 대한 세포독성을 MTT assay로 비교한 결과 $50{\mu}g/mL$의 농도에서 난소암 세포인 SK-OV-3는 70%의 억제율을 나타낸 반면 정상세포에서는 10% 미만으로 나타나 정상세포보다 종양세포에 대하여 보다 큰 선택 독성을 나타내는 것으로 보인다. Antimicrobial and antitumor activities of Camellia sinensis L seed extracts were investigated. Seed extracts showed antifungal activities against Candida albicans IFO 1594 and Cryptococcus neoformans. Inhibition zone of 20 mm was shown by 70% ethanol extract against C. albicans IFO 1594 at 100 mg/mL. Antifungal activity of seed extract was not decreased by heating at 80 and $100^{\circ}C$ for 30 min or at $121^{\circ}C$ for 15 min, indicating heat-stability of seed component. Growth-inhibitory effects were observed in 70 and 10% of tumor cell line SK-OV-3 and normal ceil line NIH/3T3 at $50{\mu}g/mL$, respectively.

동과자(冬瓜子)의 항암활성과 방사선에 대한 감작효과

최혜란(Hye-Ran Choi),이경호(Keyong-Ho Lee),김창한(Chang-Han Kim) 한국식품과학회 2003 한국식품과학회지 Vol.35 No.3

무선 ATM의 표준화 동향

홍재환(Jae-Hwan Hong),이경호(Keyong-Ho Lee) 대한전자공학회 1998 대한전자공학회 학술대회 Vol.1998 No.11