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생쥐에 있어서 Ethanol 처리에 의한 단위발생의 유기
한용만(Y . M . Han),백청순(C . S . Baik),이경아(K . A . Lee),이경광(K . K . Lee),정길생(K . S . Chung) 한국축산학회 1987 한국축산학회지 Vol.29 No.9
These experiments were carried out to obtain the basic informations and to establish the best conditions needed for the activation of mouse eggs by ethanol treatment. The influence of ethanol concentration and the age of eggs, time from hCG injection to recovery, on activation frequency of eggs was investigated, To activate the mouse eggs parthenogenetically, cumulus masses including eggs were released into a fresh solution of ethanol for 5min and then rinsed out 5-6 times with ethanol-free medium. After incubation of cumulus masses in the drops of medium for 5 hrs at 37℃ in the humidified atmosphere of 5% CO₂ in air, the adherent cumulus cells were removed with hyaluronidase (100unit/㎖). The types of parthenogenetic eggs were morphologically classified into haploid (H), immediate cleavage (IC) and diploid (D) under a inverted microscope. These parthenogenetic eggs were then incubated into the drops of mKRB medium for 96 hrs under the same conditions as above. The results obtained in these experiments were summarized as follows : 1. ICR female mice (1) When the eggs were treated with a variety of ethanol concentrations, 0 to 11%, the highest parthenogenetic activation rate (94.4%) was observed at 7% ethanol concentration. (2) Activation frequency was comparatively high when the eggs were recovered from the oviducts in more than 18 hrs after hCG injection. 2. (C₃H × C57BL) F₁ hybrid female mice (1) Of 341 F₁ eggs treated with 7% ethanol for 5 min at 18 hrs after hCG injection, 317 eggs (93.0%) were activated and a majority of parthenogenetic eggs were haploids (71.9%). (2) After 96 hrs of in vitro culture, the developmental rate of haploid, immediate cleavage and diploid eggs to morphologically normal morula or blastocyst stage was 16.7, 66.7 and 72.3%, respectively. (3) Of 18 haploid eggs recovered from the uteri on the 4th day of pregnancy after transfer to the oviducts of recipients, 12 eggs (60.0%) were normally developed to morula or blastocyst stage.
양전자방출단층촬영 ( PET ) 에서 회전 핀선원과 투과 및 방출 동시 영상 방법을 이용한 감쇠보정 방법 특성에 관한 고찰
김상은(S . E . Kim),이경한(K . H . Lee),이정림(J . R . Lee),최용(Y . Choi),지대윤(D . Y . Chi),신승애(S . A . Shin),김병태(B . T . Kim) 대한핵의학회 1995 핵의학 분자영상 Vol.29 No.4
N/A Attenuation correction is important in producing quantitative positron emission tomography (PET) images. Conventionally, photon attenuation effects are corrected using transmission measurements performed before tracer administration. The pre-injection transmission measurement approach may require a time delay between transmission and emission scans for the tracer studies requiring a long uptake period, about 45 minutes for F-18 deoxyglucose study. The time delay will limit patient throughput and increase the 1ikelihood of patient motion. A technique for performing simultaneous transmission and emission scans (T+E method) after the tracer injection has been validated. The T+E method substracts the emission counts contaminating the transmission measurements to produce accurate attenuation correction coefficients. This method has been evaluated in experiments using a cylindrical phantom filled with background water (5750 cc) containing 0.4 μCi/cc of F-18 fluoride ion and one insert cylinder (276 cc) containing 4.3 μCi/cc. GE AdvanceTM PET scanner and Ge-68 rotating pin sources for transmission scanning were used for this investigation. Post-injection transmission scan and emission scan were performed alternatively over time. The error in emission images corrected using post-injection transmission scan to emission images corrected using post-injection transmission scan to emission images corrected transmission scan was 2.6% at the concentration of 1.0 μCi/cc. No obvious differences in image quality and noise were apparent between the two images. The attenuation correction can be accomplished with post-injection transmission measurement using rotating pin sources and this method can significantly shorten the time between transmission and emission scans and thereby reduce the likelihood of patient motion and increase scanning throughput in PET.
양전자방출단층촬영기의 표준 성능평가 방법 : GE AdvanceTM 에 적용한 예
김상은(S . E . Kim),이경한(K . H . Lee),이정림(J . R . Lee),최용(Y . Choi),신승애(S . A . Shin),김병태(B . T . Kim),최연성(Y . S . Choe) 대한핵의학회 1996 핵의학 분자영상 Vol.30 No.4
N/A A series of performance measurements of positron emission tomography (PET) were performed following the recommendations of the Computer and Instrumentation Council of the Society of Nuclear Medicine and the National Electrical Manufacturers Association. We investigated the performance of the General Electric AdvanceTM PET. The measurements include the basic intrinsic tests of spatial resolution, scatter fraction, sensitivity, and count rate losses and randoms. They also include the tests of the accuracy of corrections: count rate linearity correction, uniformity correction, scatter correction and attenuation correction. GE AdvanceTM PET has bismuth germanate oxide crystals (4.0mm transaxial × 8.lmm axial × 30.0mm radial) in 18 rings, which form 35 imaging planes spaced by 4.25mm. The system has retractable tungsten septa 1mm thick and 12cm long. Transaxial resolution was 4.92mm FWHM in 2D and 5.14mm FWHM in 3D at the center. Average axial resolution in 2D decreased from 3.91mm FWHM at the center to 6.49mm FWHM at R=20cm. Average scatter fraction of direct and cross slices was 9.57%. Dead-time losses of 50% corresponded to a radioactivity concentration of 4.86μCi/cc and a true count rate of 519 kcps in 2D. The accuracy of count rate linearity correction was 1.84% at the activity of 4.50μCi/cc. Non-uniformity was 2.06% in 2D and 2.93% in 3D. Remnant errors after scatter correction were 0.55% in 2D and 4.12% in 3D. The errors of attenuation correction were 6.21% (air), 0.20% (water), -6.32% (teflon) in 2D and 5.00% (air), 6.94% (water), 3.01% (teflon) in 3D. The results indicate the performance of GE AdvanceTM PET scanner to be well suited for clinical and research applications.
초자화 냉동법으로 냉동.해동한 Neonatal 생쥐 난소의 생체내 동소이식 후 난포 발달에 관한 연구
이경아,이숙현,윤세진,고정재,차광열,Lee, K.A.,Lee, S.H.,Yoon, S.J.,Ko, J.J.,Cha, K.Y. 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.2
Ovarian development of the vitrified neonatal ovaries after orthotopical transplantation into the ovariectomized adult recipient mouse were observed. Ovaries were collected from the neonatal females on day of birth and grouped for fresh, vitrification for 1-minute, and 3-minute. Vitrified and thawed neonatal ovaries were orthotopically transplanted into ovarian bursa of the adult mice from which endogenous ovaries have removed just prior to the transplantation (1 minute: n=25; 3 minutes n=23). Fresh ovarian tissue transplanted (n=25) mice were included as control groups. Returning of the estrus cycles and the survival and development of the transplanted ovaries were evaluated. Intact ovaries from neonatal, and four weeks old mice were used for comparison of the ovarian development as in vivo-developed control. From 2 weeks after transplantation, 64%, 36%, and 75% of the transplanted mice showed return of the estrus cycles in fresh, 1-minute, and 3-minute groups, respectively. Four weeks after transplantation, all mice were sacrificed and ovarian tissues were recovered for histological analysis. 57.1%, 33.3%, and 64.7% mice in fresh, 1-minute, and 3-minute groups, respectively, had survived ovaries with follicles at various stages of growth from primordial to preovulatory follicles. Corpus lutea were also observed. Results of the present study suggest that 1) normal folliculogenesis has initiated in vivo after vitrification, and 2) the vitrification may be used as a preservation method for ovarian tissues for establishment of ovarian tissue bank.
이경아,이숙현,하상덕,윤세진,고정재,이우식,윤태기,차광열,Lee, K.A.,Lee, S.H.,Ha, S.D.,Yoon, S.J.,Ko, J.J.,Lee, W.S.,Yoon, T.K.,Cha, K.Y. 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.2
The present study was conducted to evaluate whether vitrification could be used for ovarian tissue preservation. The important issue here is that the vitrification is very simple, easy, and economical compared to the conventional cryopreserving method that using automatic freezing instrument. Human ovarian cortical tissues were cryopreserved by vitrification with 5.5 M ethylene glycol and 1.0 M sucrose as cryoprotectant. Three points of temperature ($4^{\circ}C$, room temperature, and $37^{\circ}C$) and two points of duration (5 or 10 minutes) for cryoprotectant treatment were examined to determine the best condition for vitrification of the human ovarian cortical tissues. After thawing, viability of the isolated primordial follicles was examined by dye-exclusion method. Histological appearance of tissues before and after the cryopreservation was evaluated. There was no toxic effect of the 5.5 M ethylene glycol on the primordial follicles. However, when the tissues were treated with cryoprotectant at $37^{\circ}C$ for 10 minutes and exposed to liquid nitrogen, it seems likely that there is certain deleterious effects on the viability of the primordial follicles. The highest viability of the primordial follicles was obtained with the treatment of cryoprotectant at room temperature for 10 minutes. Follicles and oocytes survived after freezing and thawing had the similar normal shapes as was seen in the specimens before cryopreservation. It would be useful to apply vitrification in establishing ovarian tissue banking for clinical purposes.