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이갑용 ( Kab-yong Lee ),송원호 ( Won-ho Song ),강장석 ( Jang-suk Kang ),이재희 ( Jae-hee Lee ),조용근 ( Yong-keun Cho ) KNU기업경영연구소 2010 기업경영리뷰 Vol.1 No.1
Korea has already entered the aging society in 2000, but with current economic and social management, a structural stagnation such as decrease in domestic demand or lack of labor is expected to occur in 2018. The issues on old people of early aging era can be categorized into economic, healthy, leisure and social-psychologic conflicts and exclusion problems. Concentrating on economic issues, it was found that the population sociological features of old people are closely related to their economic activities and that amongst those features, for the group of old people of age between 65∼69 and illiteracy, the household income, poverty status, employment status and profession status are highly relevant to the economic activities. The population sociological features of old people were found to differ depending on the degree of satisfaction in economic activities, and amongst those features, for the group of old people of the male sex and literacy of 2-year university study, the degree of satisfaction in economic activities and social relationship, and overall degree of satisfaction are found to be highly relevant to the economic activities. The education level and household income was found to give a positive effect to the degree of satisfaction and this would mean that old people of high education level and household income highly recognize the degree of satisfaction in economic activities, social relationship and in overall. Referring to this result when establishing a policy, it is expected that better quality of life by independence of old people and definition of the active senior, the insurance of new motive power by conversion to senior-friendly structure for employment and industry, the base construction for better quality of life and reinforcement of insuring system for health and protection of the aged would be encountered and suggested.
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폴리에틸렌이민 및 그들의 리포좀이 중재된 Plasmid DNA의 운반
한인숙,전미숙,이갑용,Han, In Sook,Jun, Mi Sook,Lee, Kab Yong 대한화학회 1999 대한화학회지 Vol.43 No.2
다가 양이온성 고분자인 polyethylenimine(PEI)를 이용한 plasmid DNA의 세포 전이를 검색했다. 먼저 agarose assay에 의한 2, 10, 25, 및 50KD PEI와 DNA의 중화복합체의 최적비율은 분자량에는 영향을 받지 않았고, 최적의 PEI nitrogen/DNA phosphate 중화 비율은 1.5-2.0(nmol/nmol)로 나타났다. 이 복합체들을 이용한 COS1 세포전이에서는 2KD를 제외하고는 naked DNA에 대비 전이가 증가했고, 이 중에서 특히 25KD PEI는 적정 전이조건에서 DEAE-dextran 혹은 lipofectin 보다 다소 증가된 전이율을 보였다. In vitro 세포전이의 최적 PEI/DNA 비는 7.6-13.3(nmol/nmol)이었고 최적 중화복합체를 이루는 비율보다 높게 나타났다. 용액의 pH에 따른 전이율의 변화는 크게 없었으나 산성일때가 약간 더 증가했다. 세포 표적전이와 독성감소를 위해 인지질분자를 사용한 liposome formulation을 PEI/DNA계에 도입하였다. 그 결과, PC/PE 중성 리포솜이 도입된 경우는 25KD를 제외하고는 PEI 단독일 때 혹은 리포솜 단독일 때 보다 전이율이 2-2.5 배씩 증가했다. 그러나 PEI와 같은 양이온성의 DOTAP/PE 리포솜 도입은 charge repulsion 작용으로 오히려 DOTAP/PE 단독계보다 전이가 감소하는 역효과를 보였다. Liposomal PEI계의 세포독성은 PEI 단독일 때 보다 % cell survival이 10-20% 정도 증가했다. 이 결과들은 PEI가 단독으로도 좋은 전이제로 작용 할 뿐 아니라 세포표적 운반이 가능한 중${\cdot}$음성 리포솜의 효과적인 DNA 응축제로도 이용 될 수 있음을 증명했다. The transfection efficiency of plasmid DNA was inspected using multi-cationic polymer, 5, 10, 25 and 50KD polyethylenimine (PEI). The optimal neutralization ratio of PEI/DNA complexes by agarose assay was 1.5-2.0 (nmol/nmol) without much difference in molecular weight of PEI.In vitro transfection assay, most of PEI-mediated plasmid delivery was better compared to the naked DNA. Especially, 25KD PEI at optimal condition gave higher transfection rather than the standard assay of DEAE-dextran or Lipofectin. To enhance the cell targeting delivery, the liposome formulations were introduced using phospholipids. As a result, PC/PE liposomes increased 2-2.5 times of the transfection efficiency of PEI single or PC/PE single delivery, but not the case of 25KD PEI. Moreover, the DOTAP/PE-introduced PEI delivery reduced the transfection of DOTAP/PE single delivery. All these results proved that the PEI can be used not only good transfectants and but also good DNA condensing agents in neutral/anionic liposome for cell targeting delivery.
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