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"생명윤리 및 안전에 관한 법률"이 정해준 [잔여배아보관실적대장]과 [잔여배아제공실적대장]의 작성에 관한 연구
윤산현,고용,임진호,Yoon, San-Hyun,Ko, Yong,Lim, Jin-Ho 대한생식의학회 2007 Clinical and Experimental Reproductive Medicine Vol.34 No.4
목 적: 본 연구는 연구자로 하여금 [잔여배아보관실적대장]과 [잔여배아제공실적대장]을 명확하게 작성토록 하고 관리자 및 감독자로 하여금 이를 합리적이고 통일된 관리 및 점검을 할 수 있도록 하기 위한 방법을 찾고자 한 것이었다. 연구방법: 1994년부터 2004년까지 44호 기관에 보관되어 있는 자료들을 근거로 연도별 [배아의 생성 및 이용에 관한 해당연도 현황], [연도별 잔여배아의 냉동보관과 해당연도 해동현황], [냉동배아의 해동 및 이용에 관한 해당연도 현황], [냉동배아의 제공 및 인수에 관한 해당연도 현황], [해당연도 냉동배아폐기대장], [냉동배아의 관리 및 이용에 관한 해당연도총괄대장]을 도안하고 작성하였다. 결 과: 1994년부터 2004년까지 연도별 [44호 배아의 생성 및 이용에 관한 해당연도 현황], [44호 연도별 잔여배아의 냉동보관과 해당연도 해동현황], [44호 냉동배아의 해동 및 이용에 관한 해당연도 현황], [44호 냉동배아의 제공 및 인수에 관한 해당연도 현황], [44호 해당연도 냉동배아폐기대장], [44호 냉동배아의 관리 및 이용에 관한 해당연도 총괄대장]을 구축하였던 바 해당항목들이 상호일치하고 있었다. 결 론: 본 연구에서 얻어진 결과들은 연도별 [해당기관 해당연도 잔여배아보관실적대장]과 [해당기관 해당연도 잔여배아제공실적대장]을 작성하는 기초자료로서 뿐만 아니라 [배아생성의료기관에 보관해야 할 참고문서]로도 충분하다고 사료된다. Objective: This study was to find ways to let a manager or superintendent rationally and consistently inspect as well as let a embryologist precisely record [The Book of Supernumerary Embryo Preservation] and [The Book of Supernumerary Embryo Donation]. Methods: Based on the data collected between 1994 and 2004 in Clinic 44 (Maria Fertility Hospital), [The Present State about Production and Use of Embryos], [The Preservation of Supernumerary Embryos and Their Thaw State], [The Present State about Thaw and Use of Frozen Embryos], [The Present State about Donation and Charge of Frozen Embryos], [The Book about Frozen Embryo Discard], and [The Summarization Book about Management and Use of Frozen Embryos] were designed and recorded. Results: The production, use, preservation, discard and donation quantity of human embryos, the use and discard quantity of thawed embryos, and the cumulative embryo preservation quantity could be totalized in [The Present State about Production and Use of Embryos in Clinic 44]. Also, [The Preservation of Supernumerary Embryos and Their Thaw State in Clinic 44] supported "the supernumerary embryo preservation quantity" etc. In addition, [The Present State about Thaw and Use of Frozen Embryos in Clinic 44] or [The Book about Frozen Embryo Discard in Clinic 44] supported "the use and discard quantity of thawed embryos" etc. Moreover, "The embryo donation quantity" could be totalized in [The Present State about Donation and Charge of Frozen Embryos in Clinic 44]. Finally, [The Summarization Book about Management and Use of Frozen Embryos in Clinic 44] could be used for rational and consistent management or inspection. Conclusion: The present results suggest that the documents not only be standard data to record [The Book about Supernumerary Embryo Preservation in Clinic] and [The Book about Supernumerary Embryo Donation in Clinic] but can also be preserved as treatment references.
윤산현,박세필,박태균,고대환,정길생 한국낙농학회 1989 韓國酪農學會誌 Vol.11 No.3
本 硏究는 發精牛血淸, 牛卵胞液 및 成熟卵丘細胞가 牛卵胞卵의 體外成熟과 體外受精에 미치는 效果를 調査하였으며 그 結果는 다음과 같다. 1. 發精牛血淸 50%와 成熟卵丘細胞를 添加한 m-TALP 培養液에 있어서의 卵胞卵의 成熟率은 63.7%로서 이는 基礎培養液 및 100% 發精牛血淸에 있어서의 成熟率인 40.2% 및 47.3%보다 有意하게(P<0.05) 높았다. 2. m-TALP 培養液에 牛卵胞液 50%와 成熟卵丘細胞을 添加했을 때의 卵胞卵의 成熟率은 70.5%로서 이는 基礎培養液에 있어서의 38.3%나, 100% 牛卵胞液에 있어서의 50.7%보다 有意하게(P<0.05) 높았다. 3. 發精牛血淸이나 牛卵胞液이 添加된 m-TALP 培養液에서 成熟卵丘細胞와 함께 同伴培養에 의해 成熟된 卵胞卵의 體外受精率은 22.3∼43.2%였는데 이 成績은 基礎培養液의 11.6∼17.2%보다 有意하게(P<0.01) 높았으며, 특히 25% 發精牛血淸과 25% 牛卵胞液을 添加한 培養液에서 成熟된 卵胞卵의 體外受精率은 43.2%로서 가장 높았다. These experiments were carried out to investigate the effects of estrus bovine serum(EBS), bovine follicular fluid(BFF) and matured cumulus cells(MCC) on in vitro maturation and fertilization of bovine follicular oocytes. The ovaries and testes were obtained from slaughtered Holstein cow and bulls respectively. The follicular oocytes surrounded with cumulus cells were recovered by aspirating the follicular fluid from the visible follicles of diameter 2-6 ㎜ and the semen were prepared from bull's epididymal cauda. The follicular oocytes were cultured in m-TALP medium containing EBS, BFF or/and MCC for 32h in a incubator with 5% CO_2 in air at 38℃, and then matured oocytes were again cultured for 24h, with 5×10^5/㎖ motile sperm capacitated in the BO medium containing 5㎎/㎖ BSA and 2.5㎜ caffein. The results obtained in these experiments were summarized as follow: 1. The maturation rate of the follicular oocytes, 63.7%, obtained from the m-TALP medium supplemented with 50% EBS and MCC was significantly higher (P<0.05) than that from basic medium (40.2%) or from 100% EBS medium (47.3%). 2. The maturation rate of the follicular oocytes cultured in m-TALP medium supplemented with 50% BFF and MCC was 70.5%. This value was significantly higher (P<0.05) than 38.3% of basic medium or 50.7% of 100% BFF medium. 3. The fertilization rates of the follicular oocytes matured in the m-TALP medium supplemented with EBS, BFF or/and MCC were ranged from 22.3% to 43.2%, and those values were significantly higher (P<0.01) than that of the follicular oocytes matured in basic medium. The highest fertilization rate, 43.2%, was obtained from m-TALP medium supplemented with EBS and BFF by 25% respectively.
우 난포란의 (卵胞卵) 체외성숙에 관한 연구 Ⅰ. 난포란의 회수 및 체외배양
윤산현(S . H . Yoon),고대환(D . H . Ko),박세필(S . P . Park),박태균(T . G . Park),정길생(K . S . Chung) 한국축산학회 1989 한국축산학회지 Vol.31 No.4
These experiments were carried out to develop the effective recovering method of ovarian follicular oocytes and to investigate the optimum culture conditions for in vitro maturation of recovered follicular oocytes. Bovine follicular oocytes with various configurations of cumulus cell mass were collected by aspirating follicular fluid from the ovarian follicles of various size, and then cultured in various media for 28h in a incubator with 5% C0₂ in air at 38℃. The results obtained in these experiments were summarized as follow: 1. The average number of the follicular oocytes recovered from a cow was 46.4 and the highest recovery rate was obtained from middle size follicles, 3-5㎜ in diameter. 2. The majority of the follicular oocytes with compacted cumulus cells existed in GV stage While those with dispersed cumulus cells or without cumulus cells existed between GVBD and MⅡ stage. 3. The extrusion rate of Ist polar body of the follicular oocytes cultured in Ham`s F-10, m-KRB and BMOC-3 containing hormones was not higher than those of oocytes cultured in those media without hormones. However, in case of m-TALP medium, the highest extrusion rate of Ist polar body, 77.7% was obtained by adding hormones such as FSH, HCG and Estradiol. 4. The maturation rate of the follicular oocytes cultured in basic medium without or with matured cumulus cell was 40% or 51.1%, respectively, with significant difference(p$lt;0.05).
IVF-ET Program에서 Blastocyst 배아의 발생에 관한 연구 - II. 난구세포 공동배양에 의한 Blastocyst 배아의 발생
이석원,윤산현,윤혜균,조현진,허용수,윤혜진,박세필,이원돈,임진호,Lee, Suk-Won,Yoon, San-Hyun,Yoon, Hye-Gyun,Cho, Hyon-Jin,Heo, Yong-Soo,Yoon, Hye-Jin,Park, Se-Pill,Lee, Won-Don,Lim, Jin-Ho 대한생식의학회 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.1
This study was carried out to investigate the development rates of human embryos co-cultured with cumulus cells to each blastocyst stage. Human zygotes were co-cultured on cumulus cell monolayer in YS medium supplemented with 20% hFF. On day 2, if patient had four or more "good" embryos (regular blastomeres without fragmentation), embryos were further cultured for 72hrs. Blastocysts on day 5 were classified into early blastocyst (ErB), early expanding blastocyst (EEB), middle expanding Blastocyst (MEB), and expanded blastocyst (EdB) on the basis of their morphological aspects of trophectoderm cells and blastocoele. Subsequently, maximum 3 of best blastocysts were transferred in 486 cycles. The results in this study were as follows: Patients who had four or more "good" embryos on day 2 were 498 persons, but patients whose embryos could not be transferred due to failure in development to the blastocyst stage on day 5 were 12 persons (2.4%). The development rate of embryos to the blastocyst stage was 58.2% (2,885/4,957) on day 5, and the rates that developed to the ErB, EEB, MEB, and EdB stage were 15.0% (743/4,957), 14.9% (739/4,957), 14.4% (714/ 4,957), and 13.9% (689/4,957), respectively. Total 1366 blastocysts were transferred in 486 cycles (mean number=2.81). The implantation rate and the ongoing implantation rate obtained by observing the number of G-sac and FHB were 29.9% (409/1,366) and 22.5% (308/1,366), respectively. The clinical pregnancy rate was 51.2% (249/486), and the ongoing pregnancy rate' was 39.1% (190/486). Among women showing ongoing pregnancy, women with singleton were 50% (95/190), women with twin were 37.9% (72/190), and women with triplet were 12.1% (23/190). Although triplet pregnancy rate in this study was high such as 12.1%, because many blastocysts with high viability were produced in our co-culture system using cumulus cells on day 5, we really believe that a multiple pregnancy except twin should not occur by selecting good embryos for maximum two blastocyst transfer. These results demonstrate that autologous cumulus cells may be used for the production of blastocysts with high developmental competence, and the use of autologous cumulus cells to be collected easily, and to be treated conveniently at OPU must be an effective means for obtaining high implantation and pregnancy rate.