Preparations and morphology of polyvinylalcohol/polyethyleneoxide composite nanofibers for dye-sensitized solar cells

원두현,박성해,최형주,김정헌,박현우,정성훈,김지언,이진국,김미라 한국고분자학회 2009 한국고분자학회 학술대회 연구논문 초록집 Vol.2009 No.10

Preparations of PVDF-HFP/PMMA composite nanofibers for Dye-sensitized solar cells

원두현,박성해,황원필,장성일,김정헌,정성훈,김지언,이진국,김미라 한국공업화학회 2010 한국공업화학회 연구논문 초록집 Vol.2010 No.-

삼지구엽초 추출물의 항균 및 항산화 활성에 대한 연구

원두현 ( Doo Hyun Won ),구현아 ( Hyun A Gu ),김혜진 ( Hye Jin Kim ),한샛별 ( Saet Byeol Han ),박진오 ( Jin Oh Park ),박수남 ( Soo Nam Park ) 한국미생물생명공학회(구 한국산업미생물학회) 2013 한국미생물·생명공학회지 Vol.41 No.3

In this study, the antibacterial and antioxidative activities of Epimedium koreanum Nakai were investigated for applications as cosmetic ingredients. Minimum inhibitory concentrations (MICs) of fraction-bacterium, that showed high antibacterial activity from disc diffusion assay on human skin pathogens, were tested. The ethyl acetate fraction on Staphylococcus aureus, Bacillus subtilis, Propionibacterium acnes and 50% ethanol extract on S. aureus exhibited higher antibacterial activities than methyl paraben, well known as a preservative. The DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activities of 3 fractions of E. koreanum Nakai were lower than (+)-α-tocopherol, known as a typical antioxidant. From the results of the scavenging activities of various ROS generated in Fe3+-EDTA/H2O2 systems (OSC50), 50% ethanol extract (OSC50 = 2.46 ± 0.06 μg/ml) and aglycone fraction (OSC50 = 1.45 ± 0.02 μg/ml) showed high activities similar to L-ascorbic acid (OSC50 = 1.50 ± 0.85 μg/ml), used as reference. The cellular protective effects (τ50) on photohemolysis by 1O2 generated by photosensitization reaction were tested. The cellular protective effect of 50% ethanol extract (τ50 = 37.0 ± 0.3 min) was similar to (+)-α-tocopherol (τ50 = 38.0 ± 1.8 min), used as reference. In particular, the τ50 of aglycone fraction results were 165.9 ± 7.2 min. This is a high cellular protective effect, more than 4 times that of (+)-α-tocopherol. These results indicate that E. koreanum Nakai extract, and its fractions, could be utilized as a cosmetic ingredient possessing antibacterial and antioxidative activities.

생강나무 추출물의 항산화 효과 및 타이로시네이즈 저해 활성

원두현 ( Doo Hyun Won ),한샛별 ( Saet Byeol Han ),황준필 ( Jun Pil Hwang ),김수지 ( Su Ji Kim ),박진오 ( Jino Park ),박수남 ( Soo Nam Park ) 대한화장품학회 2012 대한화장품학회지 Vol.38 No.4

본 연구의 목적은 생강나무 추출물의 항산화 활성과 타이로시네이즈 저해 활성을 살펴봄으로써 화장품 원료로서의 응용 가능성을 확인하는 것이다. 모든 실험은 생강나무의 50 % 에탄올 추출물, 에틸아세테이트(ethyl acetate) 분획, 아글리콘(aglycone) 분획을 이용하여 진행하였다. 에틸아세테이트 분획의 DPPH (1,1-diphenyl-2-picrylhydrazyl) 소거활성은 기존에 잘 알려져 있는 항산화제인 (+)-α-tocopherol 보다 높은 것으로 나타났다. Fe³⁺-EDTA/ H₂O₂ 계에서 생성된 활성산소종에 대한 세 분획의 소거활성(총항산화능)은 대표적인 항산화제인 L-ascorbic acid와 비슷한 것으로 나타났다. Rose-bengal로 증감된 ¹O₂에 의한 적혈구 파괴에서, 50 % 에탄올 추출물과 에틸아세테이트 분획의 세포보호 효과는 농도 의존적(1 ∼ 25 μg/mL)으로 증가하였다. 10 μg/mL 농도를 기준으로 비교하였을 때 에틸아세테이트 분획의 τ₅₀은 361.0 min으로 가장 높은 세포 보호 활성을 나타내었다. 타이로시네이즈 저해활성에서 에틸아세티이트 분획과 아글리콘 분획은 알부틴보다 높은 저해 효과를 나타내었다. 이상의 결과들로부터 생각나무 추출물은 활성산소종을 소거하는 항산화제로 여러 산업 분야에 응용 가능할 것이라 생각된다. 또한 알부틴을 대체하는 미백 기능성 소재로서 응용될 수 있는 가능성을 확인하였다. In this study, the antioxidative effects and inhibitory activities on tyrosinase of Lindera obtusiloba Blume (L. obtusiloba Blume) extracts were investigated. 50 % ethanol extract, ethyl acetate and aglycone fractions of L. obtusiloba Blume were used in experiments. The DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activities of ethyl acetate fraction of L. obtusiloba Blume was higher than (+)-α-tocopherol, known as a typical antioxidant. Reactive oxygen species (ROS) scavenging activities of extract and fractions of L. obtusiloba Blume on ROS generated in Fe³⁺-EDTA/H₂O₂ system were similar to L-ascorbic acid, well known as a strong antioxidant. The cellular protective effects of 50 % ethanol extract and ethyl acetate of L. obtusiloba Blume on the rose-bengal sensitized photohemolysis of human erythrocytes were increased in a concentration dependent manner (1 ~ 25 μg/mL). Ethyl acetate fraction in 10 μg/mL concentration showed the most protective effect among extracts (τ₅₀ = 361.0 min). The inhibitory effects on tyrosinase of ethyl acetate and aglycone fractions were higher than arbutin, known as a whitening agent. These results indicate that L. obtusiloba Blume extracts can be used as antioxidant, and could be applicable to functional cosmetic ingredient.

염의 농도에 따른 DOPC 리포좀의 안정성에 관한 연구

원두현 ( Doo Hyun Won ),김선영 ( Sun Young Kim ),임규남 ( Gyu Nam Lim ),박수남 ( Soo Nam Park ) 대한화장품학회 2011 대한화장품학회지 Vol.37 No.1

본 연구에서는 DOPC 리포좀의 안정성에 대한 염의 영향을 알아보기 위하여 증류수, phosphate buffer, 그리고 phosphate bufferd saline으로 리포좀을 제조하였다. 그리고 염의 추가에 따른 리포좀의 입자크기 및 제타전위 등 기본적인 물성 변화를 확인하였다. 그에 앞서 희석에 대한 영향을 알아보고자 리포좀을 각각 제조시 용매로 40배와 80배로 희석하였고 그 결과 같은 용매로 희석시킬 경우 40배 희석까지는 안정한 것을 확인하였다. 증류수로 제조한 리포좀을 증류수, phosphate buffer, 그리고 phosphate buffered saline으로 희석하였고, phosphate buffer로 제조한 리포좀은 phosphate buffer, 그리고 phosphate buffered saline으로 희석하여 염 농도 증가에 따른 리포좀의 입자크기 및 제타전위 변화를 확인하였다. 그 결과 리포좀 분산액 내에 존재하는 염의 양이 증가할수록 리포좀의 입자크기가 약간씩 작아지는 경향을 나타내었고, 제타전위 또한 염의 양이 증가할수록 0에 가까워지는 경향을 나타내었다. 결론적으로, 리포좀의 입자크기와 제타전위는 리포좀의 안정성을 판단할 수 있는 좋은 요소이며 또한 리포좀 제조시의 용매와 다른 조건의 염농도는 리포좀 입자의 안정성에 큰 영향을 줄 수 있다고 사료된다. In this study, DOPC liposomes were prepared with distilled water, phosphate buffer and phosphate buffered saline to evaluate the effects of salt on the stability of DOPC liposome. The changes in physical properties (like particle size and zeta potential) of liposome were measured after adding the salt. Liposomes were diluted 40 times and 80 times with hydration solvent to confirm the effect of dilution. Consequently, the stability of liposome was maintained up to 40 times dilution with hydration solvent. The liposome that prepared with distilled water was diluted with distilled water, phosphate buffer and phosphate buffered saline, and the liposome that prepared with phosphate buffer was diluted with phosphate buffer and phosphate buffered saline to evaluate the salt-induced changes in particle size and zeta potentia. As results, the particle size increased slightly and zeta potential became closer to 0 when the salt concentration was increased. In conclusion, particle size and zeta potential of liposome could be reasonable factors to evaluate the stability of liposome. In addition, we suggest that salt concentration of hydration solvent has a significant effect on the stability of liposome.

땅빈대 추출물의 세포 보호 효과 및 성분 분석에 관한 연구

김선영,원두현,임명선,박수남 한국생약학회 2010 생약학회지 Vol.41 No.4

In this study, the cellular protective effect, antioxidative property and component analysis of Euphorbia humifusa extracts were investigated. The ethyl acetate fraction (3.68 µg/mL) and aglycone fraction (3.15 µg/mL) of Euphorbia humifusa extract showed prominent free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (FSC50). Reactive oxygen species (ROS) scavenging activity (OSC50) of Euphorbia humifusa extract on ROS generated in Fe 3+-EDTA/H2O2 system was investigated using the luminol-dependent chemiluminescence assay. The ethyl acetate fraction (0.43 µg/mL) and aglycone fraction (0.35 µg/mL) of extract showed higher ROS scavenging activity than L-ascorbic acid (1.50 µg/mL). The cellular protective effects of fractions of Euphorbia humifusa extract on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The ethyl acetate fraction and aglycone fraction of extract protected cellular membranes against ROS in a concentration dependent manner (5~25 µg/mL), and was more effective than (+)-α-tocopherol, lipid peroxidation chain blocker. Aglycone fraction from Euphorbia humifusa extract showed 2 bands in TLC and 2 peaks in HPLC. In HPLC chromatogram of aglycone fraction, peak 1 and peak 2 were identified as quercetin and kaempferol, respectively. And these components are very effective as antioxidant. Thus, these results indicate that fractions of Euphorbia humifusa extracts can function as antioxidant in biological systems, particularly skin exposed to UV radiation by scavenging 1O2 and other ROS, and protect cellular membranes against ROS. Fractions of Euphorbia humifusa extracts can be applicable to new functional cosmetics for antioxidant.

Cellular protective effects of dehydroeffusol isolated from Juncus effusus L. and the mechanisms underlying these effects

박수남,원두현,황준필,한샛별 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.5

The purpose of this study was to identify the substance responsible for significant cellular protective effect of the extract from Juncus effusus L. on the photohemolysis of erythrocyte and to investigate the mechanism underlying this effect. The single substance was identified as ‘‘dehydroeffusol’’. To investigate the mechanism, cellular protective effect, singlet oxygen quenching rate constant, free radical and reactive oxygen species (ROS) scavenging activity and erythrocyte membrane penetration efficiency were tested. The results showed that ROS incidentally produced in the Type II reaction is the main reason for photohemolysis and that erythrocyte membrane penetration ability is crucial to cell protection.

Gel Electrolyte based on nonyl phenol Ethoxylate for Dye sensitized solar cells

조상은,김정현,원두현,박현우,박성해,황원필,이진국,김미라 한국고분자학회 2010 한국고분자학회 학술대회 연구논문 초록집 Vol.2010 No.4

골풀 부위별 추출물의 항산화 효과

최창환 ( Chang Hwan Choi ),원두현 ( Doo Hyun Won ),황준필 ( Jun Pil Hwang ),박수남 ( Soo Nam Park ) 대한화장품학회 2012 대한화장품학회지 Vol.38 No.3

이번 연구에서 우리는 골풀의 부위별 추출물의 항산화 활성을 측정하였다. 골풀의 부위는 상층부와 하층부, 고갱이 부위로 결정하였다. 실험은 골풀 50 % 에탄올 추출물, 에틸아세테이트(ethyl acetate) 분획, 아글리콘(aglycone) 분획 세 가지로 진행하였다. 항산화 활성 중 라디칼인 DPPH (1,1-diphenyl-2- picrylhydrazyl) 소거활성(FSC₅₀)을 살펴본 결과 50 % 에탄올 추출물의 경우 고갱이 부위(42.9 μ g/mL)가, 에틸아세테이트 분획은 지하 부위(12.1 μ g/mL), 아글리콘 분획은 지하 부위(12.1 μ g/mL)가 가장 높은 라디칼 소거능을 나타내었다. Luminol 발광법을 이용한 Fe³⁺-EDTA/H₂O₂ 계에서 생성된 활성산소종에 대한 소거활성(총 항산화능, OSC₅₀)을 살펴본 결과, 50 % 에탄올 추출물은 고갱이 부위(0.29 μ g/mL), 에틸아세테이트 분획은 지하 부위(0.25 μ g/mL), 아글리콘 분획은 고갱이 부위(0.20 μ g/mL)가 가장 높은 총 항산화능을 나타내었다. Rose-bengal로 증감된 ¹O₂에 의한 적혈구 파괴에 대한 세포보호 효과는 10 μ g/mL 농도에서 고갱이 부위의 아글리콘 분획이 τ⁵⁰ 321.0 min으로 가장 높은 세포 보호 활성을 나타내었다. 이와 같은 항산화 효과로 볼 때 골풀의 지하 부분과 고갱이 부위의 추출물은 천연 항산화제로서 여러 산업 분야에 응용 가능할 것이라 생각된다. 특히 높은 τ50 값을 갖는 것으로 보아, 태양광 노출에 의해서 ¹O₂이 많이 생성되는 부위인 피부를 효과적으로 보호해줄 수 있을 것으로 생각되어진다. In this study, the antioxidative effects of extracts from different parts of Juncus effusus L. were investigated. The three parts (above-ground part, below-ground part, medulla part) were selected. 50 % ethanol extract, ethyl acetate and aglycone fractions of J. effusus L. were used in experiments. The highest DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activities (FSC₅₀) was shown by medulla part (42.9 μ g/mL) in 50 % ethanol extracts, below-ground part (12.1 μ g/mL) in ethyl acetate fractions, and below-ground part (12.1 μ g/mL) in aglycone fractions. Reactive oxygen species (ROS) scavenging activities (OSC₅₀) on ROS generated in Fe³⁺-EDTA/H₂O₂ system using the luminol-dependent chemiluminescence assay showed the most prominent effect of medulla part (0.29 μ g/mL) in 50 % ethanol extracts, below-ground part (0.25 μ g/mL) in ethyl acetate fractions, and medulla part (0.20 μ g/mL) in aglycone fractions. The cellular protective effects of extract/fractions of J. effusus L. on the rose-bengal sensitized photohemolysis of human erythrocytes were increased in a concentration dependent manner (0.5 ∼ 10 μ g/mL). Especially, aglycone fraction of medulla part at a concentration of 10 μ g/mL showed the most prominent protective effect among all extracts (τ₅₀, 321.0 min). These results indicate that extracts from below-ground part and medulla part of J. effusus L. extracts can be used as an natural antioxidant. Particularly, J. effusus L. can protect suggesting a high τ₅₀ skin where many ¹O₂ was generated by sunlight exposure.

The Depigmenting Activities of Hydroxyl Carboxamide Derivatives Containing Hydrophobic Moiety

조준철,노호식,주정협,안수미,원두현,신송석,박영호,서경도,박수남 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.4