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Production of Exopolymer with Waste-Materials from Food Processing Industry
옥민,정대영,김현숙,이진우 한국생명과학회 1998 한국생명과학회 학술발표회 Vol.20 No.-
Waste-materials from food processing industry were compared with other known carbon sources for production of microbial exopolymers. Major contents of waste-materials were glucose, maltose, maltotriose, and dextrin. Productivity of exopolymers with waste-materials by Aureobasidium pullulan ATCC 42023 was higher than other tested carbon sources including glucose and sucrose. This experiment showed new ways of microbial utilization for production of biopolymers with waste-materials.
옥민,조영수 東亞大學校 2003 東亞論叢 Vol.40 No.-
Sweet persimmon (Diospyros kaki T. cv. Fuyu) Kochujang, fermented hot pepper paste added sweet persimmon powder was during storage for 24 weeks at room temperature. Viable cell count of commercial kochujang and sweet persimmon kochujang was 4.0×10^(6) cfu/g and 1.6x10^(7) cfu/g respectively. Sensory property of kochujang added with persimmon powder was not significantly affected by storage period.
토양으로부터 분리한 Bacillus sp. WRD-1이 생산하는 extracellular Protease의 특성
옥민,김민석,서원석,차재영,조영수 한국산업미생물학회 2000 한국미생물·생명공학회지 Vol.28 No.6
토양시료로부터 비교적 저온에서도 높은 활성을 나타내는 알칼리성 protease를 생산하는 세균을 수십종 분리하였다. 분리된 세균중에서 protease활성과 성장속도면에서 가장 우수한 균주를 선별하여 WRD-1으로 명명하였으며, 형태학적, 생화학적 및 생리학적 특성을 조사한 후 Bacillus sp.로 동정하였다. WRD-1유래 protease는 20℃ 배양및 pH 9.0에서 배양된 균주로부터 높은 활성을 나타내어 저온성, 알칼리성 protease로 판명되었다. 또한, WRD-1유래 protease생산을 위한 최적 배양조건은 배양온도 10~40℃, 초기 pH 6.0~8.0 및 배양시간은 10시간으로 나타났다. Protease최적활성은 pH 9.0, 온도는 30℃였다. Alkaline bacterium producing a high protease activity at low temperature was isolated by using enrichment culture from soil samples and identified as Bacillus sp. WRD-1. Cell growth was maximal at 10 hours and the optimal initial pH and culture time of culture condition for enzyme production was pH 7 and 10 hours, respectively. Temperature range of high enzyme activity were 10~40℃. The optimal pH and temperature for the enzyme activity were pH 9 and 30℃.
Bacillus thuringiensis kurstaki HD-1 유래 미생물살충제 생산을 위한 두부공업폐수의 이용
옥민,김대진,이영춘,최용락,조영수,Ok, Min,Kim, Dae-Jin,Lee, Young-Chun,Choi, Yong-Lak,Cho, Young-Su 한국생명과학회 2002 생명과학회지 Vol.12 No.3
Bacillus thrungensis를 이용한 미생물 살충제를 생산할 목적으로 두부폐수를 이용하여 내생포자 생산능을 검토하였다. 실험에 사용한 두부폐수원액의 성상은 pH 9.8, COD 276mg/$\ell$, 전질소량 71.1mg/$\ell$, 전인량 5.52mg/$\ell$ 이며 Bacillus thrungensis균을 대량배양하기 위한 기질로서의 이용 가능성을 확인한 결과, 두부폐수원액에서 가장 높은 포자형성능이 관찰 되었으며, 두부폐수원액의 첨가량이 작을수록 포자형성은 감소하는 경향을 나타내었다. 최대 포자형 성능은 1% $K_2$HPO4i 첨가때 가장 높은 포자형 성능을 나타내었다. 두부 공업폐수를 이용한 Bacillus thrungensis의 최적배지 조건에서 시간별 포자형 성능을 확인한 결과, 동일 시간당 1.5배 높은 포자형 성능이 확인되었다. 두부폐수는 Bacillus thrungensis를 생산하기 위한 적당한 배양기질로서 이용될 수 있을 것으로 생각되어 진다. The waste-water from the industry for production of a soybean curd (the soybean curd waste-water) was investigated to use for the substrate to produce the endotoxin of Bacillus thuringiensis subsp. kurstaki HD-1 used as one of well known microbial pesticides. The pH of the soybean curd waste-water was 9.8 and its chemical oxygen demand (COD), total nitrogen (TN) and phosphate (TP) were 276.0, 71.1 and 5.5mg/$\ell$, respectively. The higher was the concentration of the soybean curd waste-water in the medium, the more endotoxin was produced. Maximal sporulation occurred at which concentration of $K_2$HPO$_4$in the medium supplied with the soybean curd waste-water was 1% (w/v). Production of the endotoxin with the optimized medium supplied with the soybean curd waste-water was 1.5 times higher than that without the soybean curd waste-water. The soybean curd waste-water was found to be suitable substrate for production of the endotoxin of Bacillus thuringiensis subsp. kurstaki HD-1.