마우스 소뇌과립층의 apoptosis를 지표로 한 진단용 초음파의 안전성 검증

오헌,이송은,양정아,조성기,정치영,손창호,김성호,Oh, Heon,Lee, Song-eun,Yang, Jung-ah,Jo, Sung-kee,Chung, Chi-young,Son, Chang-ho,Kim, Sung-ho 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.3

We have studied, by a nonisotopic in situ end-labeling(ISEL) technique, frequency of apoptosis in the external granular layer(EGL) of the cerebellum of immature mice by ${\gamma}$-rays irradiation from $^{60}Co$ or diagnostic ultrasound exposure. The total number of normal cells and cells showing morphological features of apoptosis were counted. The frequency of apoptotic cells was expressed as a percentage of the total number of cells in EGL. The extent of changes following 200 cGy(1090 cGy/min) was studied at 2, 4, 6, 8, 12, or 24 hours after exposure. The maximal frequency was found 6~8 hours after exposure. The immature mice that received 18, 36, 54, 108, 198, 396 cGy of ${\gamma}$-rays or diagnostic ultrasound(7.5MHz, 4.2mW, $I_{SPTA}=7.9mW/cm^2$, $I_{SPTA}=114.3W/cm^2$) for 10 or 30 minutes were examined 6 hours after irradiation. Measurements performed after ${\gamma}$-ray irradiation showed a dose-related increase in apoptotic cells in each of the mice studied. The dose-response curves were analyzed by a linear-quadratic model ; frequency of apoptotic cell in the EGL was y = $(0.1349{\pm}0.01175)D$+$(-0.0001522{\pm}0.0000334)D^2$+0.048($r^2$ = 0.981, D = dose in cGy). In the experiment of ultrasound exposure, the frequency of apoptotic cell was $0.106{\pm}0.130$(10 minutes exposure) and $0.167{\pm}0.220$(30 minutes exposure). We estimated the relative dose of the yield from the experiment with ultrasound by substituting the yield from ultrasound exposure into the curve from the ${\gamma}$-irradiation. The relative dose of ultrasound exposure compared with ${\gamma}$-irradiation were 0.432 cGy(10 minutes exposure) and 0.885 cGy(30 minutes exposure). We have found that there is no evidence to indicate that diagnostic ultrasound involves a significant risk.

단세포 전기영동법을 이용한 인체, 마우스 및 랫드 림프구의 방사선에 의해 유발된 DNA 손상 측정

오헌,정우희,박혜란,김성호,조성기,Oh, Heon,Jung, Uhee,Park, Hae-Ran,Kim, Sung-Ho,Jo, Sung-Kee 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.1

The alkaline single-cell gel electrophoresis (SCGE) assay, called the comet assay, has been applied to detect DNA damage induced by a number of chemicals and biological factors in vivo and in vitro. The DNA damage was analysed by tail moment (TM) and tail length (TL), which were markers of DNA strand breaks in SCGE. Human, mouse and rat peripheral blood lymphocytes (PBLs) were irradiated with different doses of $^{60}Co$ ${\gamma}$-rays, e.g. 1, 2, 4, and 8 Gy at a dose rate of 1 Gy/min. A dose-dependent increase in TM (p<0.01) and TL (p<0.01) was obtained at all the radiation doses (1-8 Gy) in human, mouse and rat PBLs. Mouse PBLs were more sensitive than human PBLs which were in turn more sensitive than rat PBLs when the treated dosages were 1 and 2 Gy. However, human PBLs were more sensitive than mouse PBLs which were in turn more sensitive than rat PBLs when the irradiation dosages were 4 and 8 Gy. Data from all three species could be fitted to a linear-quadratic model. These results indicated that there may be inherent differences in the radio-sensitivity among PBLs of mammalian species.

방사선 장해에 대한 백작약의 방호효과

오헌,박혜란,정일윤,김성호,조성기 대한방사선 방어학회 2002 방사선방어학회지 Vol.27 No.3

방사선 생체 손상에 대한 방호 효과를 나타내는 천연물을 검색하기 위한 일환으로 한의학에서 보혈양혈 탕제에 널리 사용되는 백작약 (peaonia japonica)을 열수총추출물, 에탄올분획, 조다당분획으로 나누어 방사선에 의한 산환적 손상 경감 효과를 검정하였다. 사람 림프구에서 단세포전기영동 (single-cell gel electrophoresis; comet assay)을 수행하여 DNA 손상 경감정도를 관찰하였으며, 마우스에 백작약 추출물을 투여한 다음 8 Gy의 감마선을 조사한 후 간에서 지질과산화 정도를 살펴보았다. 에탄올분획 처리군에서 높은 DNA 손상 경감효과를 확인할 수 있었으며, 지질과산화 억제작용 및 라디칼 소거효과 또한 에탄올분획이 높은 효과를 나타내어 에탄올분획이 방사선 방호에 주된 역할을 하는 것으로 사료된다. 이상의 결과로 보아 백작약은 방사선의 산화적 손상에 대하여 효과적으로 세포 DNA를 방호하고, 생체막의 주성분인 지질의 과산화를 억제하는 것으로 관찰되어 특히, 독성이 거의 없는 천연물이라는 관점에서 방사선 방호제로 적용이 가능할 것으로 사료된다. We investigated the effect of Paeonia japonica (PJ) on radiation-induced oxidative damage to macromolecules in vitro and in vivo. The PJ reduced the tail moment (TM), which was a marker of DNA strand break in single-cell gel electrophoresis (SCGE; comet assay) in the human peripheral blood lymphocytes. Lipid peroxidation in the liver of the ICR mouse, measured as malondiadehyde (MDA), was also reduced by PJ administration. Ethanol fraction of PJ was more effective than polysaccharide fraction of that on reduction of TM in SCGE and lipid peroxidation. Also, Their activities to scavenge DPPH radicals and hydroxyl radicals were observed in vitro, and the activities were due to its ethanol fraction. It is plausible that scavenging of free radicals by PJ extract may have played an important role in providing the protection against the radiation-induced damage. These results indicated that Paeonia japonica might be a useful radioprotector, especially since it is a relatively nontoxic natural product.

성숙 및 신생마우스에서 아포프토시스를 이용한 방사선 피폭의 생물학적 지표

오헌,이송은,양정아,정규식,현병화,김성호,Oh, Heon,Lee, Song-eun,Yang, Jung-ah,Jeong, Kyu-shik,Hyun, Byung-hwa,Kim, Sung-ho 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.4

We have studied, by a nonisotopic in situ DNA end-labeling (ISEL) technique, frequency of apoptosis in the external granular layer (EGL) of the cerebellum after whole-body irradiation of newborn mice and intestinal crypt cell of adult mice by gamma-rays from $^{60}Co$. The extent of changes following 2 Gy(10.9 Gy/min) was studied at 2, 4, 6, 8, 12, or 24h after exposure. The maximal frequency was found 4-8h after exposure. The mice that received 0.18, 0.36, 0.54, 1.08, 1.98, or 3.96 Gy were examined 6h after irradiation. Measurements performed after irradiation showed a dose-related increase in apoptotic cells in each of the mice studied. The dose-response curves were analyzed by a linear-quadratic model; frequency(%) of apoptotic cell in the newborn mice cerebellum was ($13.49{\pm}1.175$)D+$(-1.52{\pm}0.334)D^2$+0.048($r^2=0.981$, D = dose in Gy) and frequency(number per crypt) of apoptotic cell in the intestinal crypt of adult mice was ($3.857{\pm}0.420$)D+$(-0.535{\pm}0.120)D^2$+0.155($r^2=0.952$, D = dose in Gy). It provides the basis required for a better understanding of results which will be obtained in any further studies for biological responses of radiation using newborn and adult mice.

키워드 기반 전문 검색을 이용한 Access Grid에서의 효과적인 데이터 공유 방안

오헌 ( Heon Oh ),김진승 ( Jin-seung Kim ),이영구 ( Young-koo Lee ) 한국정보처리학회 2007 한국정보처리학회 학술대회논문집 Vol.14 No.1

앞으로 다가올 유비쿼터스 컴퓨팅 환경에서는 일상생활 어디에서든지 컴퓨팅이 가능해질 것으로 예상되며, 원격지의 상대방과의 상호작용이 다양한 형태로 발전할 것으로 예상된다. 이를 지원하기 위하여 유비쿼터스 환경에서의 다자간 인터랙티브 협업 시스템들이 연구되고 있다. 그런데, 현재 개발된 다자간 협업 환경 시스템에서는 자료를 공유하는데 있어서, 데이터에 대한 인덱싱과 검색에 비중을 두지 않고 있다. 공유 데이터는 늘어나는 반면, 그것을 효과적으로 검색할 수 있는 방법이 마련되지 않아 데이터를 공유하고 활용하는 효율성이 떨어질 것이 예상됨에 따라서 키워드 검색 기반으로 데이터를 검색할 수 있는 방법을 마련하고자 한다.

감마선 및 진단용 초음파 노출에 의한 소장움세포의 apoptosis 유도

오헌(Heon Oh),이송은(Song-Eun Lee),양정아(Jung-Ah Yang),조성기(Sung-Kee Jo),손창호(Chang-Ho Son),정치영(Chi-Young Chung),김성호(Sung-Ho Kim) 한국독성학회 1999 Toxicological Research Vol.15 No.2

We have studied, by utilizing a nonisotopic in situ DNA end-labeling (ISEL) technique, frequency of apoptosis in the intestinal crypt cell of adult mice exposed to y-ray irradiation from 60CO or diagnostic ultrasound. The extent of changes following 200 cGy (1090 cGy/min) was studied at 2, 4, 6, 8, 12, or 24 hours after exposure. The maximal frequency was found 4~6 hours after exposure. The mice that received 18, 36, 54, 108, 198, 396 cGy of y-rays or diagnostic ultrasound (7.5 MHz, 4.2 mW, ISPTA =7.9 mW/㎠, ISPPA= 114.3 W/㎠) for 10 or 30 minutes were examined at 6 hours after irradiation. Measurements performed after y-ray irradiation showed a dose-related increase in proportion of apoptotic cells in each of the mice studied. The dose-response curves were analyzed by a linear-quadratic model; frequency (number per crypt) of apoptotic cells in the intestinal crypt of adult mice was (0.0386±0.004204)D+(-0.0000535±0.00001120)D²+0.15475 (r²=0.952, D=dose in cGy). In the experiment of ultrasound exposure, the frequencies of apopototic cells were 0.181±0.055 (10 minutes exposure) and 0.225±0.137(30 minutes exposure). We estimated the relative dose of the yield from the experiment with ultrasound by substituting the yield from ultrasound exposure into the curve from the y-irradiation. The relative doses of ultrasound exposure compared with y-irradiation were 0.692 cGy (IO minutes exposure) and 1.334 cGy (30 minutes exposure).

방사선조사 마우스에서 사물탕 및 사군자탕의 효과

김성호,오헌,이송은,조성기,변명우 한국수의병리학회 1997 학술대회 Vol.1997 No.-

N/A

마우스 동계골수이식 후 면역체계의 재생: I. 말초혈액 및 림프장기 세포의 숫적 변 화와 림프구아군의 분포

김성호,오헌,이송은,김순태,조성기,현병화,류시윤,Kim, Sung-Ho,Oh, Heon,Lee, Song-Eun,Kim, Soon-Tae,Jo, Sung-Kee,Hyun, Byung-Hwa,Ryu, Si-Yun,Daynes, Raymond A. 한국수의병리학회 1997 한국수의병리학회지 Vol.1 No.1

The cellularity and composition of the spleen lymph node thymus and peripheral blood and tempo of regeneration were studied at various time points after syngeneic bone marrow transplantation(BMT) in C3H/Hen mice. Significant depression of absolute lymphocyte count was noted on week 1 after lethal whole-body irradiation and BMT. In comparison to the lymph node thymus and spleen had an rapid regeneration of cellularity. The distinct cell populations($CD4^+,\;CD8^+,\;CD28^+,\;B220^+) have determined in the lymphoid tissue of mice subjected to irradiation. The relative representation of these subpopulations was significantly different from that in nonirradiated control. $CD4^+\;and\;CD8^+$ cells were present in very low numbers whereas the $B220^+$ cells reached more than normal range at 2 weeks after BMT. The number of $CD4^+$ cells returned to normal relatively soon than $CD8^+$ cell. At week 4 after BMT, the cellularity and composition of spleen lymph node and peripheral blood lymphocyte reached about 50% of the normal range therefore we can choose this time point for the other tests of immune function after BMT.

대표적 근류 생약의 방사선 방호효과

김성호,오헌,김세라,조성기,변명우,김길수,이종환,신동호,Kim, Sung-ho,Oh, Heon,Kim, Se-ra,Jo, Sung-kee,Byun, Myung-woo,Kim, Kil-soo,Lee, Jong-hwan,Shin, Dong-ho 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.1

We performed this study to determine the effect of Jiegeng(Platycodon grandiflorum), Danggui(Angelica sinensis), Gancao(Glycyrrhiza glabla), Chaihu(Bupleurum falcatnosa), Shoudehuang(Rehmannia glutinosa), Huangqi(Satragalus membranaceus), Muxiang(Saussurea lappa), Yuanzhi(Polygala tenuifolia), Rensen(Panax ginseng) and Baishaoyao(Paeonia lactiflolia), as Oriental radices herbs, on jejunal crypt survival, endogenous spleen colony formation and apoptosis in jejunal crypt cells of mice irradiated with high and low dose of ${\gamma}-radiation$. Jiegeng(p<0.005), Danggui(p<0.0005), Gancao(p<0.005), Chaihu(p<0.05), Muxiang(p<0.05), Rensen(p<0.005) and Baishaoyao(p<0.005) were effective in intestinal crypt survival. Danggui(p<0.05), Chaihu(p<0.05), Shoudehuang(p<0.05), Huangqi(p<0.05), Rensan(p<0.005) and Baishaoyao(p<0.05) increased the formation of endogenous spleen colony. The frequency of radiation induced apoptosis was also reduced by pretreatment with Chaihu(p<0.05), Muxiang(p<0.005), Yuanzhi(p<0.05), Rensan(p<0.05) and Baishaoyao(p<0.05). Although the mechanisms of this effect remain to be elucidated, these results indicated that Danggui, Chaihu, Muxiang, Rensan and Baishaiyao might be a useful radioprotector, especially since it is a relatively nontoxic natural product.

고선량 및 저선량 방사선 조사 마우스에서 누에동충하초(Paecilomyces japonica)의 효과

김세라,오헌,이해준,신동호,김종춘,박인철,오기석,조성기,김성호,Kim, Se-ra,Oh, Heon,Lee, Hae-june,Shin, Dong-ho,Kim, Jong-choon,Park, In-chul,Oh, Ki-seok,Jo, Sung-kee,Kim, Sung-ho 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.2

Cordyceps has a reputation for its broad biological activities and as a tonic which replenishs vital function in Chinese traditional medicines. As an attempt to obtain fundamental data for the development of new type Cordyceps, the effects of the fruiting bodies of cultivated fungus of Paecilomyces japonica grown on silkworm larvae on radiationinduced damages were investigated. We performed this study to determine the effect of Dongchongxiacao (Paecilomyces japonica) on jejunal crypt survival, endogenous spleen colony formation, and apoptosis in jejunal crypt cells and hair follicles of mice irradiated with high and low dose of gamma-radiation. Treatment with Dongchongxiacao showed no significant modifying effects on the jejunal crypt survival and endogenous spleen colony formation. The frequency of radiationinduced apoptosis was reduced by pretreatment of Dongchongxiacao (i.p.: 50 mg/kg of body weight, at 12 and 36 hours before irradiation, p<0.01). The spontaneous levels of apoptotic cells are $0.082{\pm}0.041$ in intestinal crypts and $0.231{\pm}0.084$ per hair follicle section of skin. Pretreatment of Dongchongxiacao was associated with decreases of 26.86% in intestinal crypt and 66.36% in hair follicle decrease in the number of cells with nuclei positively stained for apoptosis compared with the irradiation control group. We demonstrated for the first time that Dongchongxiacao administration could reduce the extent of apoptosis produced by radiation in the hair follicle. The results presented herein that Dongchongxiacao given before irradiation is capable of reducing the severity of cell loss as a result of apoptosis.