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레트로바이러스를 이용한 Tissue Inhibitor of Metalloproteinase-2 유전자 발현이 대장암 세포의 전이 및 종양형성에 미치는 영향
오일웅,정자영,장석기,이수해,김연수,손여원 대한약학회 2004 약학회지 Vol.48 No.3
Matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) playa key role in tumor invasion and metastasis. As an inhibitor of MMP-2, TIMP-2 is known to block both the invasive and metastatic behavior of cancer cells, and decrease tumor growth activity. We performed this study to investigate the effects of TIMP-2 over-expression induced by retroviral mediated gene transfer in vitro and in vivo. The human colon cancer cell line SW480 was transfected with the retroviral vector encoding TIMP-2. The effects of TIMP-2 over-expression were analyzed by invasion assay and gelatinase activity test in colon cancer cells and tumorigencity in nude mice. In evaluation of the transfection efficiency of the retroviral vector encoding TIMP-2 in colon cancer cells, we confirmed up-regulation of TIMP-2 expression dependent on the time of cell culture. In addition, inhibition of MMP-2 expression in SW480/TIMP-2 was shown by gelatin zymography. In the in vitro invasion assay SW480/TIMP-2 inhibited the invasiveness on matrigel coated with collagen. To determine whether TIMP-2 can modulate in vivo tumorigenicity and metastasis, SW480/TIMP-2 cells were injected subcutaneously in nude mice. The tumor mass formation of SW480/TIMP-2 cells in nude mice was markedly decreased compared to nontransfected cancer cells. These results showed that colon cancer cells transfected with the retroviral vector encoding TIMP-2 inhibits the invasiveness in vitro and tumorigenicity in vivo.
광합성 세균 P-99 가 분비하는 항생물질의 구조 및 항암역가에 관한 연구
하지홍,오일웅 경북대학교 유전공학연구소 1992 遺傳工學硏究所報 Vol.7 No.1
The unidentified antibiotic P-99, produced by purple nonsulfur photosynthetic bacteria was selected by antimicrobial activity and reemergency. The compound was isolated from culture broth by solvent extraction followed by silica gel column chromatography. The compound was partially characterized and shown to be chiefly biologically active against gram-positive bacteria and melanoma cell line of SK-MEL-2.
권오석,오일웅,오명진,홍승화,김찬화,안현주 대한화학회 2018 Bulletin of the Korean Chemical Society Vol.39 No.12
Monoclonal antibodies (mAbs) are the largest class of glycosylated biopharmaceuticals. Glycans are a key functional component of mAbs, and thus their characterization is highly required during mAb developmental and regulatory phases. In particular, monosaccharides are crucial for antibody efficacy, safety, cytotoxicity, and potency, so reliable monosaccharide composition assay (MCA) is required for quality control (QC) testing. Traditional methods for MCA such as HPAE-PAD and HPLC-FLD have been suffering from low sensitivity and poor reproducibility. In this study, we developed an alternative QC method for MCA of mAb with 1-Phenyl-3-methyl-5-pyrazolone (PMP) tag products using HPLC-UV platform equipped in most QC laboratories. Four characteristics including specificity, linearity, precision, and accuracy were systemically evaluated according to ICH Q2(R1) guideline. Inter-laboratory studies were also performed to validate method reproducibility and reliability. It will be a powerful platform for QC test of not only mAb products but also Fc fusion glycoprotein products.
대구·경북지방 곡류중의 Aflatoxin 생성균주의 오염에 관한 연구
김동술,문귀임,오일웅,오현숙,민충식,김은경,이삼룡,안경아,정영숙 식품의약품안전청 1998 식품의약품안전청 연보 Vol.2 No.-
대구·경북지역 농산물 중에 aflatoxin생성균주의 오염실태를 파악하기 위하여 고려, 문경, 상주, 안동, 포항, 성주, 경주, 김천, 구미, 예천, 영천, 영덕 등 12개 지역으로부터 쌀 66점, 보리 44점, 콩 94점, 당콩 36점, 조 37점, 토양 57점 등 총 334점을 수집하여 rose bengal, PDAmSLS, YES등의 배지를 사용하여 Aspergillus flavus및 parasiticus를 분리한 결과 12.6%인 42균주를 분리하엿으며 이들 중 TLC에서 형광을 나타내는 균주는 21%인 9균주였다. 또한 HPLC로 aflatoxin B₁생성능이 있는 균주를 확인하 결과 시룝점(콩)에서 분리되었으며 따라서 aflatoxin 생성능이 있는 aspergillus속 곰팡이으 ㅣ오염률은 0.3%이였다. In order to iBvestigate aflatoxin producing strains from agricultural products in the Taegu and Kyungpook dist·ricts, we collected 334 sarrlples svch as rice (66), barley(44), soybean(94), pea-nut(36), millet(37), soil(57). Using rose bengal, PDA, sLs, YES medium, we isolated the aflatoxin pro-ducing strains, fsfergiHur ffauHs and farasificur. As a result of screening by thin layer chromatography(TLC), 42 straius(21% ) from the 334 samples showed fluorescent spot. Aflatorin Bl was determinedfrom soybean(1) by HPLC- ln consequence, the contarHination percentage of fsfergiffHs sf. which pro-duce aflatoxiu sholved 0-3% .
총설 - 나노기술을 적용한 식품의 독성 및 위해성평가 현황(II)
전향숙,장현주,고상훈,오일웅,Chun, Hyang-Sook,Chang, Hyun-Joo,Ko, Sang-Hoon,Oh, Il-Ung 한국식품연구원 2011 食品技術 Vol.24 No.3
나노기술을 식품에 응용하게 되면 영양소의 전달, 식품의 색, 향미, 물성 등이 향상되고 식품 포장재나 분석에 응용하는 경우 저장성 증진이나 시료 전처리 효율 및 기기 감도를 증가시키는 것으로 알려지고 있다. 이러한 장점으로 인해 유기 및 무기 나노입자, 나노섬유, 나노에멀전, 나노크레이 등의 나노물질을 식품첨가물, 건강기능소재, 식품 접촉물질 등으로 응용하는 시도가 식품산업계에서 급속히 확산되고 있다. 나노물질의 사용범위가 다양해질수록 나노물질의 환경 및 인체 노출가능성은 높아지게 된다. 그러나 아직 나노물질의 독성 및 위해성에 대해서는 초기 단계 수준의 연구가 진행되고 있는 정도이다. 또한 나노기술의 위험 평가에 대한 신뢰성이 제고될 수 있도록 나노물질에 대한 유해성 자료의 생산뿐만 아니라 나노물질의 물리화학적 특성과 노출량을 측정할 수 있는 적절한 측정수단이 확립되어야 한다. 나아가 식품에 나노기술의 적용으로 인한 이익은 최대화하고 부작용은 최소화하여 소비자의 건강을 보호하기 위해 식품나노물질의 생산, 가공, 유통, 소비, 폐기 등에 관한 전 생애(lifecycle) 평가 및 관리가 이루어지도록 적절한 제도적 장치가 마련되어야 한다.
HIV-1 유래 렌티바이러스 벡터의 복제가능 바이러스 검출과 역가측정 분석방법 비교
장석기(Seok Kee Chang),오일웅(Il Ung Oh),정자영(Jayoung Jeong),안광수(Kwang Soo Ahn),손여원(Yeowon Sohn) 대한약학회 2005 약학회지 Vol.49 No.3
Human Immunodeficiency Virus type 1 (HIV-1) based lentivirus vector has demonstrated great potential as gene therapy vectors mediating efficient gene delivery and long-term transgene expression in both dividing and nondividing cells. However, for clinical studies it must be confirmed that vector preparations are safe and not contaminated by replication competent lentivirus (RCL) related to the parental pathogenic virus, HIV-1. In this study, we would like to establish the method for titration and RCL detection of lentivirus vector. The titration was determined by vector expression containing the green fluorescent protein, GFP in transduced cells. The titer was 1×107 Transducing Unit/ml in the GFP expression assay and 8.9×107 molecules/ml in the real-time PCR. Also, for the detection of RCL, we have used a combination method of PCR and p24 antigen detection. First, PBS/psi and VSV-G region in the genomic DNA of transduced cells was detected by PCR assay. Second, transfer and expression of the HIV-1 gag gene was detected by p24 ELISA. In an attempt to amplify any RCL, the transduced cells were cultured for 3 weeks (amplification phase) and the supernatant of amplified transduced cell was used for the second transduction to determine whether a true RCL was present (indicator phase). Analysis of cells and supernatant at day 6 in indicator phase were negative for PBS/psi, VSV-G, and p24 antigen. These results suggest that they are not mobilized and therefore there are no RCL in amplification phase. Thus, real-time PCR is a reliable and sensitive method for titration and RCL detection of lentivirus vector.