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오승범 한국감성과학회 2016 춘계학술대회 Vol.2016 No.-

Differential expression of the enzymes regulating myosin light chain phosphorylation are responsible for the slower relaxation of pulmonary artery than mesenteric artery in rats

오승범,Cho Suhan,Kim Hyun Jong,김성준 대한약리학회 2024 The Korean Journal of Physiology & Pharmacology Vol.28 No.1

While arterial tone is generally determined by the phosphorylation of Ser 19 in myosin light chain (p-MLC2), Thr 18/Ser 19 diphosphorylation of MLC2 (pp-MLC2) has been suggested to hinder the relaxation of smooth muscle. In a dual-wire myography of rodent pulmonary artery (PA) and mesenteric artery (MA), we noticed significantly slower relaxation in PA than in MA after 80 mM KCl-induced condition(80K-contraction). Thus, we investigated the MLC2 phosphorylation and the expression levels of its regulatory enzymes; soluble guanylate cyclase (sGC), Rho-A dependent kinase (ROCK) and myosin light chain phosphatase target regulatory subunit (MYPT1). Immunoblotting showed higher sGC-α and ROCK2 in PA than MA, while sGC-β and MYPT1 levels were higher in MA than in PA. Interestingly, the level of pp-MLC2 was higher in PA than in MA without stimulation. In the 80K-contraction state, the levels of p-MLC2 and pp-MLC2 were commonly increased. Treatment with the ROCK inhibitor (Y27632, 10μM) reversed the higher pp-MLC2 in PA. In the myography study, pharmacological inhibition of sGC (ODQ, 10μM) slowed relaxation during washout, which was more pronounced in PA than in MA. The simultaneous treatment of Y27632 and ODQ reversed the impaired relaxation in PA and MA. Although treatment of PA with Y27632 alone could increase the rate of relaxation, it was still slower than that of MA without Y27632 treatment. Taken together, we suggest that the higher ROCK and lower MYPT in PA would have induced the higher level of MLC2 phosphorylation, which is responsible for the characteristic slow relaxation in PA.

흰쥐에서 연령 증가에 따른 근위축 유발 관련 단백질의 변화

오승범,구재웅,차광석,성동준 한국웰니스학회 2020 한국웰니스학회지 Vol.15 No.4

The purpose of the present study was to compare age-specific and tissue-specific by comparing muscle atrophy-related protein expression according to the age difference in SD rat models. Male SD rats were assigned to groups of 3 months, 12 months, and 17 months, and protein expression was confirmed using western blots analysis in ventricles, tibialis anterior and soleus muscles. First, growth differentiation factor 8 (GDF8) showed significantly higher expression level (p<0.05) in all observed tissues as the age increased. GDF11 showed a significant increase only in the 17 months soleus muscle (p<0.05 vs 3 months). In addition, atrogin-1, except for the heart, was observed to have a high age-dependent expression level in the tibialis anterior and soleus. Conversely, SMAD2/3 showed significantly lower expression levels in 12 months and 17 months compared to 3 months in the tibialis anterior muscle (p<0.05). Calpain 1, calpain 2, and MURF-1 did not show differences between groups. These results indicated that the expression of proteins in muscle differs depending on the tissue with increasing age. Therefore, it is necessary to clearly identify target proteins for preventing muscle atrophy. 본 연구는 SD rat 모델의 달령 차이에 따른 근육위축관련 단백질 발현을 심장과 근섬유 유형에 따라 비교하여 연령-특이성과 조직 특이성 단백질을 관찰하고자 하였다. 수컷 SD rat을 3개월령, 12개월령, 그리고 17개월령 그룹으로 할당하고 심실, 앞정강근, 그리고 가자미근에서 웨스턴 블롯을 이용하여 단백질 발현을 확인하였다. 첫 번째로 growth differentiation factor 8(GDF8)은 달령이 높을수록 모든 관찰조직에서 유의하게 높은 발현량(p<0.05)을 나타냈다. GDF11은 17개월령 가자미근에서만 유의한 증가를 보였다(p<0.05 vs 3개월령). 또한 atrogin-1은 심장을 제외하고 앞정강근과 가자미근에서 달령-의존적으로 높은 발현량이 관찰되었다. 이와 상반되게 SMAD2/3은 앞정강근에서 3개월령과 비교하여 12개월과 17개월령에서 유의하게 낮은 발현량을 나타냈다(p<0.05). calpain 1, calpain 2, 그리고 MURF-1은 그룹간 차이가 나타나지 않았다. 이와 같은 결과는 연령 증가에 따라 근육내 단백질 발현이 조직에 따라 상이하기 때문에 근위축을 예방하기 위한 표적 단백질의 명확한 규명이 필요함을 의미한다.

아르바이트 활동 속 소통을 보완하는 메신저 기반의 애플리케이션 제안

오승범(Seongbeom Oh),김도연(Doyeon Kim),정성현(Sunghyun Jung),이지윤(Jiyun Lee),연명흠(Myeong-Heum Yeoun) 한국디자인학회 2023 한국디자인학회 학술발표대회 논문집 Vol.2023 No.10

성장분화인자—11에 의한 근아세포에서 근관세포로의 분화 억제

구재웅,오승범,차광석,성동준 한국웰니스학회 2020 한국웰니스학회지 Vol.15 No.2

This study aims to observe the role of growth differentiation factor 11 (GDF11) in differentiation from myoblasts to myotubes. In this study, C2C12 cells, mice myoblasts, were assigned to a total of 6 groups, a control group, a differentiation group and a differentiation group treated with two concentrations of GDF8 and GDF11, respectively. In this study, Western blot was used to confirm the protein expression level, which is an observation variable. GDF11 and GDF8 significantly inhibited myoD expression (p<0.05). In contrast, GDF11 and GDF8 treatments did not affect the expression of atrogin-1 compared to the differentiation group. Similarly, it did not affect AKT expression and phosphorylation. However, significant (p <0.05) expression inhibition of GLUT4 was shown by GDF11 treatment. In addition, it was confirmed that both mTOR and p38 proteins are inhibited by GDF11. These results indicated that in vitro conditions, GDF11 inhibits myoblast differentiation, thereby delaying the formation of myotubes. 본 연구는 근아세포에서 근관세포로 분화에 있어서 성장분화인자 11(GDF11)의 역할을 관찰하는데 목적을 두고 있다. 마우스 근아세포인 C2C12세포를 이용하여 대조군, 분화군과 각각 두 농도의 GDF8, GDF11 처치 한 분화군, 총 6그룹으로 할당하였다. 본 연구에서 관찰변인인 단백질 발현정도를 확인하기 위하여 웨스턴 블롯을 이용하였다. GDF11과 GDF8은 myoD의 발현을 유의하게 억제하였다(p<0.05). 이와 대조적으로 GDF11과 GDF8처치는 분화군과 비교하여 atrogin-1의 발현에는 영향을 미치지 않았다. 이와 유사하게 AKT 발현 및 인산화에도 영향을 미치지 않았다. 하지만 GLUT4의 유의한(p<0.05) 발현 억제가 GDF11처치에 의해 나타났다. 또한 mTOR와 p38 단백질 모두 GDF11에 의해 억제되는 것을 확인하였다. 이와 같은 결과는 실험관내 조건에서 GDF11은 근아세포의 분화를 억제함으로서 근관세포의 형성을 지연시키는 것을 나타낸다.

Inhibition of Various K+ Current by Ofloxacin in Neonatal rat Ventricular Myocytes

박상웅,오승범,구재웅,성동준 한국웰니스학회 2018 한국웰니스학회지 Vol.13 No.3

Fluoroquinolones are among the most widely used broad-spectrum antibiotics. Although ofloxacin is deemed to be relatively safe, whether side effects occur in the heart is unclear. This pilot study investigated the effect of ofloxacin on various K+ channel currents as well as L-type Ca2+ channel currents. The various K+currents were examined with a voltage pulse protocol using multi-step pulses, with a step to-120mV followed by a depolarizing step to +50mV and then to –40mV from holding potential of –80mV. L-type Ca2+ currents were measured at 0mV after prepulse to –40mV. Ofloxacin inhibited the various K+ currents, with IC50 values of 850μM for IKir, 6.8μM for IKs, and 0.4μM for IKr. Ofloxacin did not affect the L-type Ca2+ currents. We suggest that potential cardiac side effects, such as action potential prolongation and arrhythmia, maybe induced by ofloxacin.

흰쥐의 장간막동맥과 흉추대동맥에서 젖산에 의한 혈관 이완 효과

김기현,오승범,구재응,성동준 한국웰니스학회 2018 한국웰니스학회지 Vol.13 No.1

Lactate is considered a vasodilator, however, its mechanism of vasodilation is unclear. Therefore, this study attempted to elucidate the vasodilatation by lactate in thoracic aorta and mesenteric artery. In this study, isometric tension and patch-clamp experiments were performed to investigate the lactate vasodilatory response in thoracic aorta and mesenteric artery in male Spraque Dawley (SD) rats. The results obtained from this study are as follows. Lactate-induced vasodilation of the thoracic aorta was induced independently of vascular endothelial cells and Ca2+-activated K+ channels. Similarly, vasodilation of the mesenteric artery by lactate was not affected by vascular endothelial cells and pH adjusted conditions. Finally, voltage-dependent K+ currents in mesenteric artery smooth muscle cells were not increased by lactate. These results suggest that the vasodilation of the thoracic aorta and the mesenteric artery by lactate is independent of endothelial cell and pH and may lead to signal transduction in smooth muscle cells. Therefore, in future studies, a multifaceted study on cell signaling will be needed to identify lactate-induced vasodilation responses. 젖산염은 국소혈관이완물질로 알려져 있지만 현재까지 그 기전이 명확하지 않다. 따라서 본 연구는 흉추대동맥과 장간막동맥에서 젖산염에 의한 혈관이완기전을 규명하기 시도되었다. 이 연구에서는 수컷 Spraque Dawley(SD) rat의 흉추대동맥, 장간막 동맥, 그리고 장간막동맥 평활근세포에서 젖산의 반응을 알아보기 위하여 등척성 수축실험과 patch-clamp 실험을 수행하였다. 본 연구결과를 통해 확인한 결과는 다음과 같다. 젖산염에 의한 흉추대동맥의 이완은 혈관내피세포 및 Ca2+-의존성 K+통로에 비의존적으로 유도되었다. 이와 유사하게 젖산염에 의한 장간막동맥의 이완은 혈관내피세포 및 pH보정조건에 영향을 받지 않았다. 마지막으로 장간막동맥 평활근세포의 전압-의존성 K+전류는 젖산염에 의해 증가되지 않았다. 이상의 결과를 종합하면 젖산염에 의한 흉추대동맥과 장간막동맥의 혈관이완은 내피세포 및 pH에 비의존적이며, 평활근세포의 신호기전을 경유할 가능성이 제시된다. 따라서 추후 연구에서 젖산염으로 유도된 혈관 이완반응을 규명하기 위해 세포신호체계에 대한 다각적 연구가 필요할 것이다.

Protective effect of low-intensity treadmill exercise against acetylcholine-calcium chloride-induced atrial fibrillation in mice

성동준,전용균,최재일,김보경,Shadi Golpasandi,박상웅,오승범,배영민 대한약리학회 2022 The Korean Journal of Physiology & Pharmacology Vol.26 No.5

Atrial fibrillation (AF) is the most common supraventricular arrhythmia, and it corresponds highly with exercise intensity. Here, we induced AF in mice using acetylcholine (ACh)-CaCl2 for 7 days and aimed to determine the appropriate exercise intensity (no, low, moderate, high) to protect against AF by running the mice at different intensities for 4 weeks before the AF induction by ACh-CaCl2. We examined the AF-induced atrial remodeling using electrocardiogram, patch-clamp, and immunohistochemistry. After the AF induction, heart rate, % increase of heart rate, and heart weight/body weight ratio were significantly higher in all the four AF groups than in the normal control; highest in the high-ex AF and lowest in the low-ex (lower than the no-ex AF), which indicates that low-ex treated the AF. Consistent with these changes, G protein-gated inwardly rectifying K+ currents, which were induced by ACh, increased in an exercise intensity-dependent manner and were lower in the low-ex AF than the no-ex AF. The peak level of Ca2+ current (at 0 mV) increased also in an exercise intensity-dependent manner and the inactivation time constants were shorter in all AF groups except for the low-ex AF group, in which the time constant was similar to that of the control. Finally, action potential duration was shorter in all the four AF groups than in the normal control; shortest in the high-ex AF and longest in the low-ex AF. Taken together, we conclude that low-intensity exercise protects the heart from AF, whereas high-intensity exercise might exacerbate AF.

Inhibition of Various K⁺ Current by Ofloxacin in Neonatal rat Ventricular Myocytes

Park Sang Woong(박상웅),Oh Seung Bum(오승범),Gu Jaewoong(구재웅),Sung Dong Jun(성동준) 한국웰니스학회 2018 한국웰니스학회지 Vol.13 No.3

플루오로 퀴놀론은 광범위하게 사용되는 항생제 중 하나이다. Ofloxacin은 상대적으로 안전하다고 여겨지지만 심장에 부작용이 있는지 여부는 불분명하다. 이 파일럿 연구는 ofloxacin이 L-typeCa<SUP>2+</SUP> 채널 전류뿐만 아니라 다양한 K⁺ 채널 전류에 미치는 영향을 연구했다. 다양한 K⁺ 전류는 다단계 펄스를 사용하는 전압 펄스 프로토콜로 검사되었으며, 단계 -120mV에서 +50mV까지의 탈분극 단계,-80mV의 유지력에서 -40mV까지 진행되었다.L-typeCa<SUP>2+</SUP> 전류는 prepulse후 -40mV에서 0mV에서 측정되었다. Ofloxacin은 IKir의 경우 850μM, IK의 경우 6.8μM 및 IKr의 경우 0.4μM의 IC50값으로 다양한 K⁺ 전류를 억제하였다. 그러나 Ofloxacin은 L-typeCa<SUP>2+</SUP> 전류에 영향을 미치지 않았다. 이 결과를 통해 잠재적인 심장 부작용, 예를 들어 활동 전위의 연장과 부정맥이 ofloxacin에 의해 유발 될 수 있다고 제안한다. Fluoroquinolones are among the most widely used broad-spectrum antibiotics. Although ofloxacin is deemed to be relatively safe, whether side effects occur in the heart is unclear. This pilot study investigated the effect of ofloxacin on various K+ channel currents as well as L-type Ca2+ channel currents. The various K+ currents were examined with a voltage pulse protocol using multi-step pulses, with a step to-120mV followed by a depolarizing step to +50mV and then to–40mV from holding potential of-80mV. L-type Ca2+ currents were measured at 0mV after prepulse to-40mV. Ofloxacin inhibited the various K+ currents, with IC50 values of 850μM for IKir, 6.8μM for IKs, and 0.4μM for IKr. Ofloxacin did not affect the L-type Ca2+ currents. We suggest that potential cardiac side effects, such as action potential prolongation and arrhythmia, maybe induced by ofloxacin.

Fever Phobia in Korean Caregivers and Its Clinical Implications

곽영호,한승백,김도균,장혜영,김진주,류정민,오승범,이의정,이지숙,이진희,정진희 대한의학회 2013 Journal of Korean medical science Vol.28 No.11

Fever is the most common complaint among children brought into the emergency department (ED). ‘Fever phobia’ is a descriptive term for an unrealistic concern about the consequences of fever. ‘Fever phobia’ is prevalent among parents and even healthcare providers, worldwide. The aim of this study was to determine the implications of fever-phobic ideas in Korean caregivers. A prospective, multi-center survey was conducted on Korean caregivers who visited the EDs with febrile children. In total, 746 caregivers were enrolled. The mean age of the subjects was 34.7 yr (SD±5.0). Three hundred sixty respondents (48.3%) believed that the body temperature of febrile children can reach higher than 42.0°C. Unrealistic concerns about the improbable complications of fever, such as brain damage, unconsciousness, and loss of hearing/vision were believed by 295 (39.5%), 66 (8.8%), and 58 (7.8%) caregivers, respectively. Four hundred ninety-four (66.2%) guardians woke children to give antipyretics. These findings suggest that fever phobia is a substantial burden for Korean caregivers.