[추억의 생일카드] 짧은 만남, 긴 추억 / [유머세계일주] 자동차광은 못 말려

오미현,편집부(편집자) 샘터사 1990 월간 샘터 Vol.21 No.7

LC-MS/MS를 이용한 건강기능식품 중 실리마린 분석법 연구

오미현,이진희,김상아,김미혜,Oh, Mihyune,Lee, Jin Hee,Kim, Sang-A,Kim, Meehye 한국식품위생안전성학회 2018 한국식품위생안전성학회지 Vol.33 No.2

건강기능식품의 경우 단성분보다는 복합제품 개발이 우선시되고, 12가지 기본제형이외에도 즉석밥과 같은 일반식품형태의 제품으로 범위가 확대되고 있다. 이러한 제품에서 기능성(지표)성분을 효율적으로 분석할 수 있는 방법에 대한 연구가 필요한 실정이다. 복합제품 등에서 정성 정량 가능한 밀크씨슬 추출물의 기능성(지표)성분인 실리마린을 효과적으로 분석할 수 방법을 확립하기 위하여 본 연구를 수행하였다. 액체크로마토그래프-질량분석기(LC-MS/MS)를 이용하여 신속하고 효율적으로 실리마린의 6가지 구성성분, 실리크리스틴, 실리디아닌, 실리빈A, 실리빈B, 이소실리빈A, 이소실리빈B를 동시분석할 수 있는 정성 정량시험법을 확립하였다. 확립된 시험법에 대해 특이성, 검출한계, 정량한계, 정확도, 정밀도에 대한 검증을 통하여 유효성을 확인하였다. 또한 시중 유통검체 17건을 확보하여 시험한 적용가능성도 검토하였다. 직선성에서는 $r^2$ > 0.999 이상임을 확인하였고, 검출한계 및 정량한계는 각각 실리크리스틴은 0.3, 0.8, 실리디아닌은 9.0, 27.3, 실리빈A은 3.3, 9.9, 실리빈B는 5.4, 16.3, 이소실리빈A는 4.9, 14.9, 이소실리빈B는 3.2, $9.7{\mu}g/L$이었다. 또한 평균 회수율은 96.2~98.6%로 나타났으며, 반복정밀도는 상대표준편차 3%이하, 실험실간 재현성은 1.5%이하로 나타나 정확성, 재현성이 우수하였으며 이는 AOAC 가이드라인에서 제시한 기준에 모두 적합한 수준이었다. 시중 유통품에 대하여 실험한 결과 81.2~106.5%로 모두 적합하였다. 따라서 본 연구에서 개발된 분석법은 건강기능식품 중 실리마린의 정성 정량분석에 이용될 수 있으며, 나아가 건강기능식품 공전 개정에 반영되어 우수한 품질의 건강기능식품유통에 기여할 것으로 판단된다. The Ministry of Food and Drug Safety (MFDS) is amending its test methods for the use of health functional foods (dietary food supplement), in order to establish regulatory standards and specifications in Korea. In this regard, we continue to pursue and perform our research on the analytical method development for the items being researched and reviewed. In this study, we have developed a sensitive and selective test method that could simultaneously separate and determinate six major bioactive flavonolignans in silymarin, which are based on the use of a liquid chromatographic-tandem mass spectrometry (LC-MS/MS). The standard calibration curves presented a linearity effect with the correlation coefficient ($r^2$) > 0.999. The limits of detection (LODs) and limits of quantitation (LOQs) were in the range of $0.3{\sim}9.0{\mu}g/L$ and $0.8{\sim}27.3{\mu}g/L$, respectively. The recovery results ranged between 96.2~98.6% at 3 different concentration levels, and its relative standard deviations (RSDs) were less than 5% as noted in this study. The proposed analytical method was characterized with a noted high resolution of the individual silymarin constituents, and the assay was fully validated as well. Our research can provide a significant scientific evidence that can be useful to amend the silymarin test method for the Health Functional Food Code.

문화가 디자인사고에 미치는 영향에 관한 연구 - 우치다 시게루 작품을 중심으로 -

오미현 한국문화공간건축학회 2016 한국문화공간건축학회논문집 Vol.- No.55

This study analyzes the relationship between culture and design on how culture affects designers’ thoughts and expressions. Based upon literature reviews and pre-studies, culture is epitomized into its definition, characteristics and stages of development. Case studies also help to identify designers’ works along with their cultural thoughts. Differences in culture represent the differences in ideas, while education plays key roles in making the ideas into use as well as acquiring skills for applications. Cultural design thinking is formed via divergent thoughts by designers who have experienced or contemplated with diverse cultural backgrounds. It is also defined as a relationship among culture, design thinking and its expression. These kinds of thoughts are influenced in the design process and designers melt culture into their works, visual or non-visual alike. Uchida Shigeru’s design is expressed by in-depth understanding of spiritual, physical and behavioral stages of culture, which is applied into spaces in Japanese ways, reflecting its physical and spiritual traditions.

Naf Nanotube가 첨부된 복합레진의 미세구조와 불소유출정도에 관한 연구

오미현,임범순,이용근,양형철,이상훈 대한치과재료학회 2008 대한치과기재학회 학술대회 Vol.2008 No.11

Session I - 천연물 신약 심사평가 및 현황

오미현 한국에프디시규제과학회(구 한국에프디시법제학회) 2007 한국에프디시법제학회 학술대회 Vol.2007 No.1

Study on Consumer Exposure to Sun Spray and Sun Cream in South Korea

오미현,김서영,한지은,박소담,Go Un Kim,안수선 한국독성학회 2019 Toxicological Research Vol.35 No.4

When conducting risk assessments of cosmetic ingredients, it is important that reliable exposure information is obtained for cosmetic products. As cosmetics are becoming more diverse, continuous effort must be made to obtain exposure data that reflect their growth and usage trends. The usage pattern of cosmetics, such as the application area and amount used, may differ by product type and also by country. We conducted a survey to compare the amount of sun spray and sun cream used in a usage environment in South Korea. The study was conducted on Haeundae Beach, one of the most popular beaches in South Korea. A total of 1,255 beachgoers participated in this study; 604 and 651 participants used the sun spray and sun cream, respectively, while sunbathing and enjoying water activities on the beach for one day. Exposure was analyzed following a probabilistic method. On comparing all subjects, it was found that the group that used sun spray (mean: 44.52 g/day) used significantly more product (p = 0.000) than those who used sun cream (mean: 20.51 g/day). By analyzing the daily exposure of sun spray and sun cream per unit body weight according to age and gender, the exposure amount of sun spray and sun cream was found to be highest among 2~9 year-old girls (mean for sun spray: 2.51 g/kg/day, p95: 5.50 g/kg/day, mean for sun cream: 0.79 g/kg/day, p95: 1.79 g/kg/day). The amount of sun spray used is approximately twice that of sun cream. Among both the sun spray and sun cream groups, the exposure amount per unit body weight was highest in girls younger than 10. These factors should be considered when conducting risk assessments of sun spray and sun cream.

고령자의 치유를 위한 가상공간의 접근 방향성 연구 - 환경의 지각과 인지 과정 중심으로-

오미현 한국공간디자인학회 2022 한국공간디자인학회논문집 Vol.17 No.3

(연구배경 및 목적) 현재 대부분의 가상현실 공간은 시각디자인을 중심으로 한 스토리텔링의 구현이 중심이 되고 있다. 하지만 향후 점차 증가할 가상공간의 디자인은 가상의 현실을 실제처럼 디자인하는 것으로 공간디자인에 필요한 지식을 기반으로 공간에 접근하는 것이 필요하다. 특히나 신체와 심신 기능이 일반인보다 저하되는 고령자에게는 가상공간을 통해 간접경험을 할 수 있어 매우 긍정적인 효과를 주고 있다. 본 연구의 목적은 공간디자인관점으로 접근한 가상공간 분야의 전문성 향상과 고령자의 심신치유를 위한 가상공간의 발전에 기여하는 것을 목표로 고령자의 치유를 위한 가상공간의 방향성에 관해 연구를 진행하였다. (연구방법) 연구의 내용은 첫째, 치유환경과 가상공간에 관한 이론 고찰을 통해 특성을 살펴보고 고령자의 특성에 맞는 관계성을 모색한다. 둘째, 가상공간의 인지 과정을 환경지각의 개념에서 살펴보고 이것에 의해 사용자에게 미치는 영향을 분석한다. 셋째, 가상공간이 고령자의 치유에 효과적이다는 가정하에 고령자의 치유를 위한 가상공간의 방향성을 제안하였다. 연구의 방법은 문헌고찰, 사례분석 등을 통하여 관계성을 탐구하는 정성적 방법으로 연구결과를 도출하였다. 이를 위해 연구의 이론 고찰의 범위는 환경심리학, 인지심리학, 환경계획, 영상학에서 살펴보았으며, 최근 10년 이내 개발된 고령자를 위한 가상현실의 사례조사를 진행하였다. 문헌에 대한 시간적 범위는 최근 10년 이내 2012년~ 2022년의 데이터를 활용하였다. (결과) 고령자 치유를 위한 가상공간의 방향성은 1단계: 고령자의 특성을 우선으로 고려, 2단계: 고령자의 환경지각(다감각)의 요소를 분석, 3단계: 고령자의 경험에 대한 정보, 지식, 이미지를 수집, 4단계: 고령자의 가상공간의 콘텐츠를 계획하는 방향성으로 진행될 수 있다. (결론) 연구의 결론은 첫째, 고령자의 가상공간은 치유를 목적으로 진행되어야 하며 과거의 경험으로부터 자극되는 감각지각을 고려해야 한다. 둘째, 사용자는 가상공간에서 자신의 과거 경험으로부터 기억을 소환하여 감각을 자극받게 되며 이 경우 유사한 경험과 환경의 그룹에서는 유사한 결과를 도출할 수 있다.

해외논문고찰을 통한 천식 및 기침${\cdot}$가래 치료제의 유효성 평가지표 개발연구

오미현,이지형,장보형,정승기,선승호,고호연,전찬용,박종형,고성규,Oh, Mi-Hyune,Lee, Ji-Hyung,Jang, Bo-Hyoung,Jung, Sung-Ki,Sun, Seung-Ho,Ko, Ho-Yeon,Jun, Chan-Yong,Park, Jong-Hyeong,Ko, Seong-Gyu 대한한방내과학회 2007 大韓韓方內科學會誌 Vol.28 No.3

Objectives : The purpose of this study was to develop outcome indicators in clinical trials of herbal drugs effective for asthma. cough and sputum. To accomplish the objective, this study collected outcome indicators developed and used according to conventional medical concepts. Methods : Our research group reviewed SCI papers concerned with developing outcome indicators to evaluate amelioration of asthma, cough and sputum. We also reviewed clinical trials of herbal drugs effective for them. Results : To evaluate asthma, objective as well as subjective methods were chosen according to the purpose of each trial. Objective methods were PEF, FEVl, serum IgE, peripheral eosinophil counts, and so on. Subjective methods were symptom scores, symptom diaries, quality of life measures, etc. To evaluate cough and sputum, objective and subjective methods were also chosen. Objective methods were tussigenic challenges, sputum induction and computerized methodology, and subjective methods were similar to the methodology evaluating asthmatic symptoms. Conclusions : It is desirable for a clinical trial evaluating herbal drugs for asthma, cough and sputum to use objective and subjective outcome indicators together. However, biological outcome indicators, a kind of objective methods, can not be chosen as the purpose of trial. Valid and reliable subjective outcome indicators are needed to develop good clinical trials of herbal drugs effective for asthma, cough and sputum.

문화차이를 고려한 디자인 사고 방법에 관한 연구 - 동양과 서양의 문화차이 비교를 중심으로 -

오미현 한국문화공간건축학회 2016 한국문화공간건축학회논문집 Vol.- No.56

This study was a research about the relationship between culture and design. It explored the influence caused by cultural differences on design thinking method, and searched for cultural design thinking method accordingly. The purpose of this study was for design learners and persons concerned to recognize the importance of cultures and design and enhance. Design thinking skills that can overcome cultural barriers. For the research methodology, advanced research and literature review were conducted to explore cultures and analyze Eastern and Western cultural characteristics. Based on this, 64 students of a graduate school of design (27 Asians, 27 Westerners), who came from Eastern and Western backgrounds. They were selected as the experiment targets. An experiment was conducted on the differences of cultural thinking between the two different backgrounds. The subjects received cards with childhood experiences and an alignment board. In case of cards that met their personal experiences and sympathy. They placed them on the column with a high score but for cards that they could not relate to, they arranged them on the column with a low score. The subjects displayed responses where they were reminded of their own experiences and formed a bond of sympathy. They showed characteristics of cultural thinking for the relationship between the target of the card, and their own experiences regardless of the score of the card. However, cultural differences were displayed passively for simple visual elements. In conclusion, this study identified that cultural differences causes an influence on the formation of divergent thinking for designers. Accordingly, cultural design process is suggested as a methodology of design thinking. This methodology can be used as a guideline for the enhancement of design thinking education that can succeed cultures. Also be applied as a methodology of design works.

북한 동포 어린이에게

오미현 한국국어교육학회 1996 세계가 한동네 Vol.1996 No.-