Diversity analysis of Saccharomyces cerevisiae isolated from natural sources by multilocus sequence typing (MLST)

엄유정,손수영,정동현,허문석,김창무,박선영,신우창,이상진,어중혁,김계원,박천석 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.4

We used multilocus sequence typing (MLST) to analyze the diversity of natural isolates of Saccharomyces cerevisiae, the most important microorganism in alcoholic fermentation. Six loci, ADP1, RPN2, GLN4, ACC1, MET4, and NUP116, in S. cerevisiae genome were selected as MLST markers. To investigate genetic diversity within S. cerevisiae, 42 S. cerevisiae isolated from natural sources in Korea as well as six S. cerevisiae obtained from Genbank and four industrial S. cerevisiae were examined using MLST. Twenty-six polymorphic sites were found in the six loci. Among them, ACC1 had the most genetic variation with eight polymorphic sites. MLST differentiated the 52 strains into three clades. Alcohol fermentation results revealed that S. cerevisiae in Clade III produced less alcohol than those in Clades I and II. These results suggested that MLST is a powerful tool to differentiate S. cerevisiae and can potentially be used to select S. cerevisiae suitable for industrial use.

토끼 두개골에서 새로 개발된 biphasic calcium phosphate의 골형성 효과 : A pilot study

엄유정,홍지연,김성태,이용호,박상현,박준효,조규성,김종관,최성호,Um, Yoo-Jung,Hong, Ji-Yeon,Kim, Sung-Tae,Lee, Yong-Ho,Park, Sang-Hyun,Park, Sun-Hyo,Cho, Kyoo-Sung,Chai, Jung-Kiu,Kim, Chong-Kwan,Choi, Seong-Ho 대한치주과학회 2008 Journal of Periodontal & Implant Science Vol.38 No.2

Purpose: Biphasic calcium phosphates have been of great interest recently. Mixing adequate ratios of hydroxyapatite(HA) and beta-tricalcium phosphate($\beta$-TCP) allowed to control the resorption rate without distorting its osteoconductive property. This study evaluated the bone formation effect of newly developed biphasic calcium phosphate(BCP) in calvarial defect of rabbits. Materials and Methods: 6 male New Zealand rabbits were used. Four defects with 8mm in diameter were created on each animal. BCP with HA/$\beta$-TCP ratio of 7:3 and particle size of $0.5{\sim}1.0\;mm$ was used as the test group and bovine bone with $0.25{\sim}1.0\;mm$ particle size, as the control group. Both test and control group materials were randomly implanted in the calvarial defects and were covered witha polymer membrane. The animals were sacrificed after 12, 24, and 48 weeks of implantation under general euthanasia. Resin blocks were obtained and were stained by masson's trichrome for histological observation. Results: Overall results were uneventful without any defect exposure or inflammation. The amount of new bone formation and bone maturity increased with increase in healing period at both groups. New bone in test group was mostly formed along the material particle surrounded by osteoblasts, and observation of osteoblastic stream was also present. Bone maturity increased as it was closer to thedefect margins. Under the same healing period, the test group showed more bone formation than the control group with more stable bovine bone particles remaining even after 48 weeks, whereas considerable resorption took place in BCP. Almost total defect closure was observed in test group with new bone formation in the central part of the defect. However, limited new bone formation was observed in the control group. Conclusion: Within the limits of the study, the present study reveals the newly developed BCP to be a good osteoconductive material. However, further studies are needed to be conducted in a different study model with a larger sample size.

The Evaluation of Calcium Phosphate Coated Implants by Ion-beam Assisted Deposition (IBAD) Method in Dogs : A Preliminary Study

엄유정,Jung-Suk Lee,Ui-Won Jung,Sung-Tae Kim,김창성,정성민,이인섭,최성호 한국생체재료학회 2009 생체재료학회지 Vol.13 No.1

Rough surfaced implants have been reported to favor early bone healing than smooth surfaced implants. Amongvarious methods introduced to roughen the implant surface, coating with calcium phosphate(CaP) is one of them. The purpose of this study was to evaluate the bone healing response of anodized implants with two different coatingthickness of CaP(200 nm and 500 nm) deposited by ion beam assisted method. CaP coated implants wereplaced on circumferencial coronal defects of four mongrel dogs and the amount of defect fill was evaluated histologicallyafter 8 weeks. The amount of coronal defect filling varied among the implants according to its surface characteristics. The CaP coating thickness of 500 nm showed the greatest amount of defect fill than implants with200 nm of CaP coating thickness and non coated implants. Within the limits of this study, when coating implantswith CaP by ion-beam assisted deposition method, coating thickness of 500 nm seemed to be effective.

단백질 전달 영역 융합-Bone Morphogenetic Protein-2가 백서 두개골 결손부에서 골 조직 재생에 미치는 효과

엄유정,조규성,김종관,최성호,채중규,김창성,Um, Yoo-Jung,Cho, Kyoo-Sung,Kim, Chong-Kwan,Choi, Seong-Ho,Chai, Jung-Kiu,Kim, Chang-Sung 대한치주과학회 2008 Journal of Periodontal & Implant Science Vol.38 No.2

Purpose: Recombining bone morphogenetic protein (BMP) is usually acquiredfrom high level animals. Though this method is effective, its high cost limits its use. The purpose of this study was to evaluate the effect of bone morphogenetic protein-2 with protein transduction domain (BMP-2/PTD;TATBMP-2) on bone regeneration. Rat calvarial defect model and osteoblastic differentiation model using MC3T3 cell were used for the purpose of the study. Materials and Methods: MC3T3 cells were cultured until they reached a confluence stage. The cells were treated with 0, 0.1, 1, 10, 100, 500 ng/ml of BMP-2/PTD for 21 days and at the end of the treatment, osteoblastic differentiation was evaluated usingvon Kossa staining. An 8mm, calvarial, critical-size osteotomy defect was created in each of 48 male Spraque-Dawley rats (weight $250{\sim}300\;g$). Three groups of 16 animals each received either BMP-2/PTD (0.05mg/ml) in a collagen carrier, collagen only, or negative surgical control. And each group was divided into 2 and 8 weeks healing intervals. The groups were evaluated by histologic analysis(8 animals/group/healing intervals) Result: In osteoblastic differentiation evaluation test, a stimulatory effect of BMP-2/PTD was observed in 10ng/ml of BMP-2/PTD with no observation of dose-dependent manner. The BMP-2/PTD group showed enhanced local bone formation in the rat calvarial defect at 2 weeks. New bone was observed at the defect margin and central area of the defect. However, new bone formation was observed only in 50% of animals used for 2weeks. In addition, there was no new bone formation observed at 8 weeks. Conclusion: The results of the present study indicated that BMP-2/PTD(TATBMP-2) have an positive effect on the bone formation in vitro and in vivo. However, further study should be conducted for the reproducibility of the outcomes.

형태가 변화하는 디자인의 유사 여부 판단

엄유정 전북대학교 법학전문대학원 학술지 편집위원회 2018 JEONBUK LAW JOURNAL Vol.7 No.1

The influence of diabetes mellitus on periodontal tissues: a pilot study

엄유정,정의원,김창성,박은정,차정헌,유윤정,최성호 대한치주과학회 2010 Journal of Periodontal & Implant Science Vol.40 No.2

Purpose: The purpose of this study was to preliminarily evaluate the influence of diabetes mellitus (DM) on periodontal tissue without establishment of periodontitis. Methods: Seven-week-old db/db mice were used for the diabetic experimental group and systematically healthy mice of the same age were used as controls. After 1 week of acclimatization, the animals were sacrificed for hard and soft tissue evaluation. The pattern of bone destruction was evaluated by stereomicroscope evaluation with alizarin red staining and radiographic evaluation by microscopic computerized tomography images. Histological evaluation was performed with hematoxylin and eosin stain for evaluation of soft tissue changes. Results: In both stereomicroscope evaluation and radiograph image analysis, aggressive form of bone destruction was observed in diabetic animals when compared to the systematically healthy controls. In histological evaluation, apical migration of junctional epithelium with slight inflammatory cell infiltration was observed with disarrangement of connective tissue fi-bers. Conclusions: Within the limits of this study, diabetic animals presented distortion in periodontal attachment and an aggressive bone loss pattern when compared to the healthy controls, suggesting that DM has an independent effect on periodontal tissue destruction irrespective of the presence or absence of periodontal disease.

복수적 기능을 가진 표장의 상표로서의 사용의 인정 범위와 상표 사용의 판단 요소에 관한 분석

엄유정 전북대학교 법학전문대학원 학술지 편집위원회 2017 JEONBUK LAW JOURNAL Vol.6 No.1

밀성박씨 경주 손곡문중 목판의 수종식별

엄유정 ( Yu-jeong Eom ),박병대 ( Byung-dae Park ) 한국목재공학회 2018 목재공학 Vol.46 No.3

This study was conducted to identify wood species of two printing woodblocks either from the Park clan’s documentary or Ji-dang documentary of Songok clan of the Milseong Park, Songok, Gyeongju, Korea. Eighty-eight woodblocks out of the total 282 woodblocks were randomly selected to compare anatomical features for the identification of wood species, using a light microscope. As a result, seven wood species were identified, and all of them were diffuse-porous hardwood species. The most significant portion, i.e., 39.8% of wood species was Carpinus laxiflora Blume. Then, Pyrus pyrifolia Nakai, Acer mono Maxim, Prunus sargentii Rehder, Tilia amurensis Rupr, Diospyros kaki Thunb, and Betula costata Trautv was 25.0%, 15.9%, 10.2%, 3.4%, 3.4% and 2.3%, respectively, indicating that all diffuse-porous hardwood species had been used for the woodblocks. It was believed that diffuse-porous hardwoods had been used because they provided an easy of engraving complex Chinese letters, of acquiring these wood species in Gyeongju areas, and a high resistance to repeated printing.

IoT 기반의 온실의 에너지 절감을 위한 모니터링 시스템

엄유정(Yu-Jeong Eom),강준구(Joon-Koo Kang),권기성(Ki-Seong Kwon),안지연(Ji-Yeon An),이선도(Seon-Do Lee),이기학(Ki-Hak Lee),이동수(Dong-Su Lee),전경한(Kyunghan Chun),김봉환(Bonghwan Kim) 대한전자공학회 2021 대한전자공학회 학술대회 Vol.2021 No.6

맥상용어목록을 이용한 맥상표현 자동추출방법 연구 : 『의종손익』을 중심으로

금유정(Keum Yujeong),이병욱(Lee Byungwook),엄동명(Eom Dongmyung),송지청(Song Jichung) 대한한의학원전학회 2020 대한한의학원전학회지 Vol.33 No.4

Objective : 한의서에 기재된 맥상표현은 맥상용어의 조합으로 구성되어 있으며 다양한 조합의 맥상용어를 통해 병증을 설명하고 있다. 하지만 한의사 또는 한의학 전문가들은 이러한 다양한 조합의 맥상표현에 대해 모두 파악하는 일은 불가능하며 개인적인 노력에 의해 한의서를 접하는 과정에서 단편적으로 맥상표현에 대한 지식을 접할수 있을 뿐이다Methods : 본 연구는 Microsoft社의 Window 10 환경에서 Microsoft Office 365의 Access를 사용해 진행하였다. 맥상표현 추출 대상 원문은 『醫宗損益』이다. 최종적으로 [맥상제외용어]를 이용하여 자동으로 추출된 맥상표현을 정제하고자 하였다. Results : 본연구를 진행할 때 PC환경은 Intel Core i7-1065G7 CPU 1.30GHz를 사용하였으며, RAM은 8GB를 사용하였고, Window 10 64비트 운영 체제를 사용하였다. 6,115개 문장에서 1차적으로 6,497개 맥상표현을 추출하였으며 정제과정을 거쳐 최종적으로 5,507개 맥상표현을 추출하였다. Conclusion : 고문헌 의서가 프로그램이 파악할 수 있는 데이터파일로 존재한다는 가정하에서 본 연구방법을 통해 맥상을 나타내는 맥상표현 지식을 DB로 작성하는 방법이 연구자가 직접 작성하는 경우보다 시간적, 인적 자원의 절약할 수 있음을 나타낸다. Objectives : Pulse descriptions in Korean Medical texts are comprised of combinations of pulse terminology, where various combinations of pulse terminology are used to describe disease symptoms. For Korean Medical doctors and professionals, however, it is impossible to identify the entirety of pulse description combinations, and their understanding is mostly limited to those learned from classical texts studied individually. Methods :This research was carried out by using Access of Microsoft Office 365 in Windows 10 of Microsoft. Pulse descriptions were extracted from the text, 『Euijongsonik』. In the final stages, the automatically extracted list of pulse descriptions was refined through [excluded terminology of pulse description]. Results : The PC environment of this research was Intel Core i7-1065G7 CPU 1.30GHz, with 8GB of RAM and a Windows 10 64bit operation system. Out of 6,115 verses 6,497 descriptions were primarily extracted, and after a refinement process, the final list contained 5,507 pulse descriptions. Conclusions : Based on the assumption that classical texts are available in data form to be processed by programs, current research methodology demonstrated that it was more efficient in regards to time and man power to create a pulse description database compared to when the researcher manually created one.