발효유제품내에서 Escherichia coli O157 : H7 과 Salmonella ser . typhimurium 의 생존

안영태,임정현,강호진,장영호,김현욱 한국식품위생안전성학회 1997 한국식품위생안전성학회지 Vol.12 No.3

Escherichia coli O157:H7 and Salmonella ser. typhimurium are pathogens involved in food poisoning in numerous countries. This study aimed to obtain knowleges on the survival of E. coli O157:H7 KSC 109 and S. ser. typhimurium ATCC 14028 in fermented milk products which were on sale in Suwon Yakult supplier. To the final concentration of 10³-10⁴ cfu/ml of E. coli O157:H7 KSC 109 or S. ser. typhimurium ATCC 14028 in the fermented milks, Metchnikoff, Ace, Yakult, Mastoni and Super100 were inoculated with these pathogens and then were stored at 4 and viable cells of these pathogens were periodically counted. The results showed that the survival of two pathogens differed in the different types of fermented milks tested. Number of surviving E. coli O157:H7 KSC 109 and S. ser. typimurium ATCC 14028 cells (initial inoculum, 10³-10⁴ cfu/ml) were decreased to 10', 102 cfu/ml in Ace after 100 hours, and were decreased gradually to 10¹ cfu/ml in Yakult after 250 hours. In the other fermented milks, viable cells of E. coli O157:H7 KSC 109 was not drastically decreased but those of S. ser. typhimurium ATCC 14028 was decreased gradually to 10² (Mastoni), and to 10¹ cfir/ml (Super100) after 250 hours. It appeared that S. ser. typhimurium ATCC 14028 was more susceptible than E. coli O157: H7 KSC 109 at low pH. Vibale cells of E. coli O157:H7 KSC 109 was not drastically decreased in most of fermented milks tested except Ace and Yakult, but in general, S. ser. typhimurxum ATCC 14028 was drastically decreased in most of the fermented milks. The major inhibition factor against these pathogens in the fermented milks during storage at 4℃ appeared to be the acidity and the metabolites produced by the starters bacteria used in fermented milk products.

중국계약법상 무역계약불이행에 관련 규정의 연구

안영태 한국통상정보학회 2006 통상정보연구 Vol.8 No.1

This study is to introduce the Chinese Contract Law against non-performance of the contract and to solve the wide range of problems involving to executing the trading contract. The parties' liability for the period of performance, the place of performance, the failure to deliver conforming goods together with it's nature of the lack of conformity, and the methods of compensation against damages and the force majeure clauses application. Those issues affect directly to commercial transactions in international business.The focus is more on the interrelationship of private individuals in its trade and on aiming to remove the legal obstacles from the Chinese Contract Law to freely flow of international trade. Reference may include foreign corrupt practices, Conventions on Contracts for the International Sale of Goods and Laws of England, France, and Japan. This study has brought the efforts of these issues in the full spectrum of performance and with concentrations on effectiveness to avoid the different viewpoints of the general principles of CISG and commercial practice founded pre-eminently.This study, in presenting the legal framework, will contribute to a better understanding of the purpose of rules of Chinese Contract -Law as they interact to the benefit of the parties involved in international trade transactions.The writer believes that a problem-oriented approach and the concentration as outlined above would offer a different perspective for law faculty teaching in this area and hope that this study can be sufficiently diverse to satisfy many of those views.

中國契約法상 貿易契約履行法規에 관한 연구

안영태 한국무역통상학회 2004 무역통상학회지 Vol.4 No.1

유산균과 건강

안영태 한국식품영양학회 2011 한국식품영양학회 학술대회논문집(학술심포지움) Vol.2011 No.12

Probiotics는 살아있는 미생물로서 섭취했을 때 숙주에게 건강증진효과를 나타내는 미생물이다. 식품 및 제약에서 가장 많이 사용되는 probiotics로는 Lactobacillus와 Bifidobacterium 균주가 있다. 이 유산균들은 장내 정상균총의 유지, 설사 예방 및 개선, 항암, 항대장염, 면역조절, 항고지혈, 항당뇨 효과 등 많은 건강증진 효과를 나타낸다. 최근에 장내 미생물이 에너지 대사, 특히 지방 대사에 영향을 주어 체중을 조절하는데 있어 중요한 역할을 한다는 것이 알려졌다. 이와 더불어 몇몇 probiotic Lactobacillus가 비만 실험동물 뿐만 아니라 비만인에서도 체지방을 조절하는 것으로 나타나 당뇨 및 비만 등과 같은 대사성질환의 예방 및 치료에 있어 유산균의 사용 가능성이 점쳐지고 있다. 그러나 유산균의 항비만 효과 그리고 그에 관한 기전에 대해서는 많은 연구들이 수행되지 않았다. 따라서 유산균의 항비만 효과를 검증하기 위해서는 앞으로 이에 관한 기전에 대해 더 많은 연구들이 진행되어야 하고 궁극적으로 사람에서 그 효과가 검증되어야할 것이다. Probiotics are described as live micro-organisms which when administered in adequate numbers confer a health benefit on the host(FAO/WHO, 2002). The most commonly used probiotic microorganisms in foods and pharmaceuticals for human consumption are Lactobacillus and Bifidobacterium species. These lactic acid bacteria have the numerous health benefits, eg. improvement on the disturbances of the indigenous microflora, prevention or amelioration of diarrhea, anticancer, anticolitic effects, immune modulation, antihyperlipidemic effects, antidiabetic effects and so on. Most recently, it has emerged that intestinal microbes have an important role in body weight regulation by influencing energy metabolism, especially lipid metabolism. In addition, some probiotic Lactobacillus were found to be effective in regulating adipose tissue in overweight adults as well as in obese animal models. However, there are several reports about antiobesity effects of LAB and their mechanisms. Thus further studies are needed to determine their mechanism and to confirm their efficacy in humans as well.

한국 성인의 분과 유제품에서 발견된 젖산균의 복합담즙산염 분해에 관한 연구

안영태,김현욱 한국축산학회 1999 한국축산학회지 Vol.41 No.2

Bile salt deconjugation of one hundred-thirty lactic acid bacterial isolates from human feces, fermented milk products, and probiotic capsules was investigated on MRS media containing oxgall, bile extract(porcine), bile (bovine), TCA(taurocholic acid), TCDCA(taurochenodeoxycholic acid), TDCA(taurodeoxycholic acid), GCA (glycocholic acid), GCDCA(glycochenodeoxycholic acid), and GDCA(glycodeoxycholic acid). Lactobacillus fermentum, L. brnvis, L. plantarum, and Lactococcus lactis ssp. lactis had higher deconjugation activity of bile salts than L. acidophilus strains. When bile salts were deconjugated, halos of precipitate around colonies or opaque granular white colonies appeared on MRS agar containing bile salts. Opaque granular white colonies were formed when TDCA and GDCA were deconjugated. No or weak deconjugation activity was found under aerobic conditions. Fifty-three lactic acid bacterial isolates from human feces and thirteen isolates from fermented milk products or probiotic capsules were found to have high deconjugation activity of bile salts. When deconjugation of bile salts by selected lactic acid bacteria was tested on taurine- and glycine-conjugated bile acids, L. acidophilus FMP 0101 and L. axidophilus HF 0404 deconjugated all the conjugated bile acids tested except for GCDCA and GDCA, and these two strains deconjugated more taurine- than glycine-conjugated bile acid. E. faecium HP 0201 isolated from commercial probiotic capsules had the highest deconjugation activity among the examined bacterial isolates. Optimum pH for the bile salt hydrolase of the two L. acidophilus strains appeared to be pH 5.0-5.4 and the bile salt hydrolase activity of both L. acidophilus strains from human feces was higher inside the cell than in the supernatant without bile extract. When bile extract was added to the media, the enzyme activity of L. acidophilus HF 0401 was high both inside the cell and in the supernatant, but L. acidophilus HF 0404 exhibited higher enzyme activity in the supernatant than inside the cell.

Initiator가 삽입된 Na+-montmorillonite(MMT)를 라디칼 중합 개시제로 사용한 PMMA-Clay 나노 복합재료의 제조 및 그 물성에 관한 연구

안영태,권건호,조아영,정한모 한국고분자학회 2004 한국고분자학회 학술대회 연구논문 초록집 Vol.2004 No.04

젖산균과 비피더스균에 의한 Escherichia coli O157 : H7 과 Salmonella typhimurium 의 생장억제

안영태,신필기,김현욱 한국식품위생안전성학회 1997 한국식품위생안전성학회지 Vol.12 No.3

Lactobacillus acidophilus NCFM, Lactobacillus casei YIT 9018, Bifidobacterium longum 8001, and Bifadobacterium longum 8025 at the level of 10^6 cfu/ml were cultured with 10⁴ cfu/ml of Escherichia colt O157:H7 KSC 109 or Salmonella typhimurium ATCC 14028, in order to verify the effects of lactic acid bacteria and bifidobacteria on the growth of the pathogens. In the mixed culture of lactic acid bacteria with E. colt O157:H7 KSC 109, growth inhibition and atypical microcolonies of E. colt O157:H7 KSC 109 were observed. The pathogens inoculated grew for 5 hours (pH 5.3), by the time L. acidophilus NCFM reached the exponential growth phase, and then the surviving pathogens were decreased to 10¹ cfu/ml after 35 hours. When L. casei YIT 9018 was grown with the pathogens, they grew for 10 hours (pH 4.6), by the time L. casei YIT 9018 reached the end of exponential growth phase, and then the surviving pathogens were decreased drastically. Up to the stationary growth phase of lactic acid bacteria, L. acidophilus NCFM exhibited stronger inhibition against the pathogens than L. casei YIT 9018 did, which might be attributed to its faster growth. Likewise bifidobacteria inhibited the growth of the pathogens. tested, bifidobaceria was weaker in the inhibitory activity than lactic acid bacteria. When Bifidobacterium longum 8001 was cultured with the pathogens, E. colt O157:H7 KSC 109 was gradually inhibited at the stationary growth phase of bifidobacteria, atypical microcolonies were formed on Levine EMB medium after 48 hours, and Salmonella grew up to 10^6 cfu/ml, then was drastically inhibited at the exponential growth phage of Bifxdobacterium longum 8001. But when Bifidobacteriuam longum 8025 was cultured with the pathogens, the pathogens grew to the same level of Bifidobacteriuam longum 8025 after 10 hours, then the surviving pathogens were decreased drastically.

저온 폐열 회수용 진동형 히트 파이프 열교환기의 성능 평가에 관한 연구

안영태,이욱현,김정훈,김종수 대한설비공학회 2001 설비공학 논문집 Vol.13 No.5

유산균의 항균 효과

안영태 한국유가공기술과학회 2008 추계 학술대회 Vol.2008 No.-

Corm Steep Liquor를 이용한 젖산균이 생산배지에 관한 연구

안영태,김근배,인영민,정석근,함준상,김동운,이경욱,김선기,김현욱 한국축산식품학회 2000 한국축산식품학회지 Vol.20 No.3

젖산균의 생장을 위한 질소, 탄소 공급원으로서 corn steep liquor의 이용 가능성을 시험하고 반응 표면 분석(Response surface metho-dology)을 이용하여 젖산균의 최적 생상 배지 조성을 연구하였다. 반응 표면 분석에서 L.fermentum의 생장배지에 첨가되 corn steep liquor와 yeast extract의 농도(p<0.01) 그리고 corn steep liquor와 yeast extract의 교호작용(P<0.05)이 L.ferm-entum의 생장에 큰 영향을 미치는 것으로 나타났으며, 이때 생균수가 최대인 corn steep liquor의 함량은 10.77%, yeast extract는 3.39%.Tween 80은 1.69%으로 예측되었다. 한편, Lc, lactis ssp. lactis 의 생장배지는 corn steep liquor 의 농도(P<0.01) 그리고 corn steep li-quor와 $\beta$-glycerophosphate disodium salt의 교호작용(P<0.05)인 Lc. lactis ssp. lactis의 생장에 큰 영향을 미치는 것으로 나타났으며 이때 생균수가 최대인 corn steep liquor의 함량은 3.5%, $\beta$-glycerophosphate disodium salt는 4.38%로 예측되었다. MRS broth와 예측된 최적 배지에서 L.fermentum의 젖산과 초산의 생성량은 각각 0.166, 0.114과 0.273, 0.081 M이고 M 17glc broth와 최적배지에서 Lc. lacti ssp. lactis의 젖산과 초산의 생성량은 각각 0.089, 0.003과 0.189, 0.003M이었다. 따라서 corn steep liquor는 L. fermentum와 Lc. lactis ssp, lactis 의 생장을 위해 질소 또는 탄소 공급원으로서 배지에 첨가 될 수 있는 우수한 농업 부산물로 판단되었다.