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건조방법을 달리한 땅콩호박의 영양성분 분석 및 생리활성 평가
심완섭,김호중,구수빈,채선희,최용원,문효,박성민,이옥환,Sim, Wan-Sup,Kim, Ho-Joong,Ku, Su-Bin,Chae, Seon-Hee,Choi, Yong-Won,Men, Xiao,Park, Sung-Min,Lee, Ok-Hwan 한국식품영양학회 2020 韓國食品營養學會誌 Vol.33 No.1
The purpose of this study was to provide the basic data of butternut squash (Cucurbita moschata) according to the drying methods. The proximate composition and chromaticity of freeze-dried and hot air-dried Cucurbita moschata powders were evaluated, and the antioxidant effects of these ethanolic extracts were analyzed by DPPH, ABTS, FRAP, reducing power activity, total phenolic, and flavonoid content. As a result, Cucurbita moschata was revealed to have high carbohydrate and crude protein contents, while crude ash and crude fat contents were relatively low. Also, it was confirmed that the hot air-dried Cucurbita moschata had lower L, a, and b values than the freeze-dried Cucurbita moschata. Additionally, as a result of analyzing the antioxidant activity of Cucurbita moschata ethanolic extracts, hot air-dried Cucurbita moschata ethanolic extract showed significantly superior antioxidant activity than freeze-dried Cucurbita moschata ethanolic extract. Also, hot air-dried Cucurbita moschata ethanolic extract was revealed to have a higher polyphenol content, but slightly lower flavonoid content than freeze-dried Cucurbita moschata ethanolic extract. Based on the data from this study, further experiments on Cucurbita moschata material are necessary.
권희연,최선일,조봉연,최승현,심완섭,한웅호,장길웅,최예은,여진희,조주현,이옥환,Kwon, Hee-Yeon,Choi, Sun-Il,Cho, Bong-Yeon,Choi, Seung-Hyun,Sim, Wan-Sup,Han, Xionggao,Jang, Gill-Woong,Choi, Ye-Eun,Yeo, Jin-Hui,Cho, Ju-Hyun,Lee, Ok-Hwan 한국식품영양학회 2019 韓國食品營養學會誌 Vol.32 No.5
The purpose of this study was to demonstrate the quality characteristics of Brassica juncea cultivated in Jeongseon (BJJ), South Korea. We analyzed the nutritional components and antioxidant activity of BJJ. As a result of the free sugar analysis, the contents of glucose and fructose in BJJ were $0.29{\pm}0.02g/100g$ and $0.10{\pm}0.00g/100g$, respectively. The major fatty acids were palmitic acid, octadecenoic acid and stearic acid. The palmitic acid was the highest at 31.22% of all fatty acids. The major minerals were identified as Ca, P, K, Mg and Na. The contents of vitamin $B_1$, vitamin $B_2$, vitamin $B_6$, vitamin C and vitamin E in BJJ were $0.02{\pm}0.00mg/100g$, $0.087{\pm}0.01mg/100g$, $0.02{\pm}0.00mg/100g$, $0.56{\pm}0.06mg/100g$ and $0.20{\pm}0.03mg\;{\alpha}-TE/100g$, respectively. As a result of the free amino acid analysis, total amino acid contents in BJJ were $2,801.21{\pm}115.38mg/100g$. L-proline content was the highest ($744.30{\pm}119.06mg/100g$) in BJJ. BJJ extract inhibits reactive oxygen species production in 3T3-L1 adipocytes. Also, BJJ extract exhibits a protective effect on oxidative stress in $H_2O_2$-induced human dermal fibroblast. These results indicate that BJJ comprises various valuable nutrients which can be used as functional food ingredients.
피부 섬유아세포에서 갈참나무 잎 추출물의 항산화 및 항노화 효능
최선일,이종석,이사라,여주홍,정태동,조봉연,최승현,심완섭,한웅호,이진하,김종대,이옥환,Choi, Sun-Il,Lee, Jong Seok,Lee, Sarah,Yeo, Joohong,Jung, Tae-Dong,Cho, Bong-Yeon,Choi, Seung-Hyun,Sim, Wan-Sup,Han, Xionggao,Lee, Jin-Ha,Kim, Jong Dai 한국식품위생안전성학회 2018 한국식품위생안전성학회지 Vol.33 No.2
본 연구는 예부터 약용으로 사용되었거나, 현재 식품공전에 식품원료로 사용이 가능한 것으로 등록된 국내 산림지역에 자생하는 식물을 식품산업에 활용하고자 선행연구에서 우수한 항산화 활성을 보인 갈참나무 잎을 본 연구에서 사용하였다. 70% 에탄올을 이용하여 추출한 갈참나무 잎을 이용하여, hydrogen peroxide로 산화적 스트레스를 유도한 피부 섬유아세포에서의 세포 보호효과, 세포내 항산화 효과 및 항노화 효과를 측정하였다. 세포 독성을 평가한 결과 25, 50 및 $100{\mu}g/mL$의 갈참나무 잎 추출물을 처리하였을 때 모두 독성을 나타내지 않았으며, hydrogen peroxide로 산화적 스트레스를 유도한 상태에서는 세포를 보호하여 농도 유의적으로 세포생존율이 증가하였다. 특히, $100{\mu}g/mL$의 농도에서는 양성대조군으로 사용한 $50{\mu}M$ ascorbic acid 수준까지 세포 생존율이 증가하였다. 세포내 항산화 효과를 확인 하기위해 사용한 $H_2-DCFDA$ assay에서는 형광현미경과 형광흡광도 측정에서 모두 농도 유의적으로 세포내 ROS 저감 활성을 확인하였고 갈참나무 잎 추출물을 $100{\mu}g/mL$ 농도로 처리했을 때는 $50{\mu}M$ ascorbic acid와 비슷한 세포내 항산화 효과를 나타내었다. 또한 SA-${\beta}$-galactosidase assay를 이용한 갈참나무 잎 추출물의 피부 섬유아세포에 대한 항노화활성은 ROS 생성 억제 효과와 유사한 경향으로 갈참나무 잎 추출물의 농도 유의적으로 세포 노화 억제효과를 확인하였다. 이상의 결과를 종합하여 볼 때 갈참나무 잎 추출물이 hydrogen peroxide로 인한 산화적 스트레스 상태에서 세포 보호효과, 항산화 효과 및 항노화 효과가 관찰되어 기능성 식품원료로서의 활용도가 매우 넓을 것으로 판단된다. The skin of the human body occupies the largest surface area of the body and acts as a protection for the person's internal organs. As such, the skin is a major target of oxidative stressors, and these oxidative stressors are known to contribute to skin aging over the course of time. For the most part, an antioxidant is an effective approach to utilize to prevent symptoms related to the reactive oxygen species (ROS)-induced aging of the skin. Therefore, we investigated the antioxidant and anti-aging activity of the leaves of the Quercusaliena Blume extract (QBE). In our study, we confirmed that the cell viability tested with XTT {2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide innersalt} assay was not affected up to a concentration of $100{\mu}g/mL$. In addition, the cell viability of HDF cells induced by hydrogen peroxide was recovered from 81% to 104% after treatment with QBE, which showed the greater protective effect than that of ascorbic acid. Treatments of QBE dose-dependently inhibited reactive oxygen species (ROS) production in HDF cells induced by hydrogen peroxide, which correlated with their protective effects on cell viability. Since QBE treatment exhibited the suppression effect of skin aging by decreasing the ROS production, QBE could be used as a not only natural anti-aging but also antioxidant resource.
이진하(Jin-Ha Lee),이혜빈(Hye-Bin Lee),최선일(Sun-Il Choi),정태동(Tae-Dong Jung),조봉연(Bong-Yeon Cho),최승현(Seung-Hyun Choi),심완섭(Wan-Sup Sim),한웅호(Xionggao Han),이옥환(Ok-Hwan Lee) 한국식품영양과학회 2017 한국식품영양과학회지 Vol.46 No.12
본 연구에서는 아마란스 안토시아닌 색소의 온도별(4, 25 및 40°C), pH별(3.0, 7.0, 및 9.0), 당에 대한 안정성을 12일간 4일 간격으로 색소 함량, 총 페놀 함량, DPPH 라디칼 소거능, 아질산염 소거능 및 FRAP를 측정하여 평가하였다. 아마란스 안토시아닌 색소는 저온 4°C과 pH 3.0에서 색소함량의 변화가 적었으며, glucose, xylose를 첨가 시 당의 첨가에 따른 색소 함량의 큰 변화는 보이지 않았으나 다른 당에 비해 fructose 첨가에 의해 색소 함량의 감소가 다소 크게 측정되었다. 아마란스의 항산화 효과를 측정한 결과 아마란스 추출물 1, 2.5, 5 mg/mL의 DPPH 라디칼 소거능은 37.67±0.87%, 57.65±0.52%, 88.09±0.10%로 평가되었고, 아질산염 소거능은 아마란스 추출물의 농도를 2.5, 5 및 10 mg/mL로 처리하였을 때 각각 61.53±0.03%, 64.54±0.46% 및 75.70±0.32%로 평가되었다. 또한, FRAP 활성은 아마란스 추출물 2.5, 5 및 10 mg/mL 농도에서 0.34±0.00, 0.40±0.01, 0.53±0.01로 추출물의 농도가 증가함에 따라 항산화 활성도 증가하였다. 이상의 결과는 향후 아마란스를 활용한 가공식품 미 천연 식품 색소 개발을 위한 기초자료로 활용할 수 있을 것으로 생각된다. This study investigated the stability of anthocyanin and antioxidant activities from amaranth baby leaf extracts. Amaranth baby leaf was examined to investigate the effects of pH, temperature, and sugars on the stability of anthocyanin. Antioxidant compounds and activities were measured based on total phenolic content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities, nitrite scavenging activity, and ferric ion reducing antioxidant power (FRAP). In the results, the stability of anthocyanin rapidly decreased at 40°C, while the stability of anthocyanin slowly decreased at 4°C. The type of sugar (glucose, fructose, xylose) did not influence the stability of anthocyanin. The stability of anthocyanin was reduced in the following order: pH 3> pH 9> pH 7.0. Total phenolic content was 5.62±0.12 mg gallic acid equivalent/g, DPPH radical scavenging activity of amaranth baby leaf extracts ranged from 37.67±0.87 to 88.09±0.10%, and FRAP value increased depending upon the concentrations of amaranth baby leaf extracts and ascorbic acid. The nitrite scavenging ability of amaranth baby leaf extracts ranged from 61.53±0.03 to 75.70±0.32%. In conclusion, amaranth baby leaf can be used as a potential source of natural antioxidant compounds and natural food colorants.