수확후 채소류에 발생하는 진균독소의 탐색과 방제 (II) 이병된 양념 채소류(양파, 마늘, 고추)에서 주요 진균독소 검출

정일민,주호종,심성철,백수봉,유승헌,Chung, Ill-Min,Ju, Ho-Jong,Sim, Sung-Chur,Paik, Su-Bong,Yu, Seung-Hun 한국분석과학회 1998 분석과학 Vol.11 No.3

수확후 Alternaria, Penicillium 및 Fusarium에 이병된 고추, 양파, 마늘을 HPLC로 주요독소들을 검정한 결과 고추에서 Alternaria 독소의 AOH(alternariol)는 소량~$400{\mu}g/g$, ALT(alternuene)는 소량~$103{\mu}g/g$, TeA(tenuagonic acid)는 $249{\sim}342{\mu}g/g$ 및 AME(alternariol monomethyl ether)는 $206{\sim}294{\mu}g/g$이 검출되었고 양파, 마늘에서 Penicillium 독소의 citrinin이 $2.8{\sim}18.4{\mu}g/g$, penicillin-G는 $0{\sim}439.0{\mu}g/g$, penicillic acid는 $0{\sim}10.2{\mu}g/g$ 및 patulin은 $0{\sim}7.0{\mu}g/g$ 검출되었다. 그리고 양파, 마늘에서 Fusarium 독소로는 fusaric acid가 $0{\sim}553.6{\mu}g/g$ 검출되었을 뿐 deoxynivalenol과 nivalenol은 검출되지 않았다. The major mycotoxins were detected from peppers, onions and garlics infected postharvest pathogens, Alternaria, Penicillium and Fusarium. Analyses of the major mycotoxins were conducted using HPLC. Detected Alternaria mycotoxins per gram of infected postharvest peppers were alternariol (AOH) with amount ranged from small quantity to $440{\mu}g/g$, altenuene (ALT) with amount ranged from small quantity to $103{\mu}g/g$, tenuagonic acid (TeA) with amount ranged from 249 to $342{\mu}g/g$ and alternariol monomethyl ether (AME) with amount ranged from 206 to $294{\mu}g/g$. Penicillium toxins per gram of infected postharvest onions and garlics were citrinin with amount ranged from 2.8 to $18.4{\mu}g/g$, penicillun-G with amount ranged from no detection to $439.0{\mu}g/g$, penicillic acid with amount ranged from no detection to small quantity and patulin with amount ranged from no detection to small quantity. Fusarium toxins per gram of infected postharvest onions and garlics were fusaric acid with amount ranged from no detection to $553.6{\mu}g/g$. However, deoxyrivalenol and nivalenol were not detected from onins and garlics infected by Fusarium.

가지과 작물 유전체 정보 검색을 통한 육종지원 컨텐츠 개발

심성철(Sung-Chur Sim),최성원(Seongwon Choi),김민경(Minkyung Kim),이주영(Juyoung Lee) 한국원예학회 2014 한국원예학회 학술발표요지 Vol.2014 No.10

토마토 품종의 형태적 특성조사 및 데이터베이스 구축

심성철(Sung-Chur Sim),나지선(Jisun Na),박지혜(Jihye Park),이다윤(Dayoon Lee) 한국원예학회 2014 한국원예학회 학술발표요지 Vol.2014 No.10

성발현 연관 분자마커를 이용한 단성화 참외 선발

방선웅(Sun-Woong Bang),송기환(Kihwan Song),심성철(Sung Chur Sim),정상민(Sang Min Chung) 한국원예학회 2016 원예과학기술지 Vol.34 No.1

멜론에서 개발된 단성화 연관 마커인 T1ex를 참외계통 선발에 적용하였다. T1ex 마커가 멜론에서는 단성화에 대해 두 가지 크기의 변이를 보였으나 참외에서는 단일 크기 변이를 보였고 하나의 계통을 제외한 나머지 105 계통에서 단성화와 양성화에 대한 표현형과 유전자형이 99% 일치함을 보였다. 또한 T1ex 단성화 연관마커의 MAS 적용가능성을 확인하기 위해 참외 품종육성에 이용되고 있는 240개 개체 중 분자마커 선택법으로 선발된 98개체를 비교해 본 결과 표현형과 유전자형이 100% 일치하였고, 이형 유전자형을 조기에 효과적으로 제거 할 수 있음을 확인하였다. 따라서 멜론에서는 적용이 어렵다고 판단된 단성화 연관 마커 T1ex가 참외에서는 계통 육성과정에서 적용 가치가 매우 높다고 평가된다. The DNA marker T1ex, originally developed from melon (Cucumis melo L.) for monoecy, was employed in chamoe, which is referred to as oriental melon. This marker shows size variations in monoecious melon. However, in chamoe, no such detrimental size variation was found in monoecious chamoe, and 99% association between flower phenotypes and genotypes of the T1ex marker was observed in 106 lines of chamoe. To evaluate the efficacy of the T1ex marker for marker-assisted selection (MAS), a total of 240 plants of chamoe breeding lines were screened using the T1ex marker. Among these, 98 varieties were selected. Although the T1ex marker might not be useful for MAS in melon, we found 100% concordance between genotypes and phenotypes for sex expression in chamoe. These results suggest that the T1ex marker will be a useful resource for MAS for monoecy in chamoe.

[농화학] 수확후 채소류에 발생하는 진균독소의 탐색과 방제 (Ⅱ) 이병된 양념 채소류(양파, 마늘, 고추)에서 주요 진균독소 검출

정일민(Ill-Min Chung),주호종(Ho-Jong Ju),심성철(Sung-Chur Sim),백수봉(Su-Bong Paik),유승헌(Seung-Hun Yu) 한국분석과학회 1998 분석과학 Vol.11 No.3

수확후 채소류에 발생하는 진균독소의 탐색과 방제(Ⅱ) 이병된 양념 채소류(양파, 마늘, 고추)에서 주요 진균독소 검출

정일민,주호종,심성철,백수봉,유승헌 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

수확후 Alternaria, Penicillium 및 Fusarium에 이병된 고추, 양파, 마늘을 HPLC로 주요독소들을 검정한 결과 고추에서 Alternaria 독소의 AOH(alternariol)는 소량∼440㎍/g 및 ALT(altenuene)는 소량∼103㎍/g, TeA(tenuagonic acid)는 249∼342㎍/g 및 AME(alternariol monomethyl ether)는 206∼294㎍/g이 검출되었고 양파, 마늘에서 Penicillium 독소의 citrinin이 2.8∼18.4㎍/g, penicillin-G는 0∼439.0㎍/g, penicillic acid는 0∼10.2㎍/g 및 patulin은 0∼7.0㎍/g 검출되었다. 그리고 양파, 마늘에서 Fusarium 독소로는 fusaric acid가 0∼553.6㎍/g 검출되었을 뿐 deoxynivalenol과 nivalenol은 검출되지 않았다. The major mycotoxins were detected from peppers, onions and garlics infected postharvest pathogens, Alternaria, Penicillium and Fusarium. Analyses of the major mycotoxins were conducted using HPLC. Detected Alternaria mycotoxins per gram of infected postharvest peppers were alternariol (AOH) with amount ranged from small quantity to 440 ㎍/g, altenuene (ALT) with amount ranged from small quantity to 103 ㎍/g, tenuagonic acid (TeA) with amount ranged from 249 to 342 ㎍/g and alternariol monomethyl ether (AME) with amount ranged from 206 to 294 ㎍/g. Penicillium toxins per gram of infected postharvest onions and garlics were citrinin with amount ranged from 2.8 to 18.4 ㎍/g, penicillun-G with amount ranged from no detection to 439.0 ㎍/g, penicillic acid with amount ranged from no detection to small quantity and patulin with amount ranged from no detection to small quantity. Fusarium toxins per gram of infected postharvest onions and garlics were fusaric acid with amount ranged from no detection to 553.6 ㎍/g. However, deoxyrivalenol and nivalenol were not detected from onins and garlics infected by Fusarium.

항균성 물질을 생산하는 Penicillium sp. KAC 균주의 배양적 특성에 따른 항균 활성

도은수,심성철,백수봉 建國大學校附設 農業資源開發硏究所 1998 農資源開發論集 Vol.20 No.-

Abstract The streai of Penicillium sp. KAC previously shown strong antifungal activity against Rhizoctonia solani AG 1-1 and AG 2-2 was cultured in liquid media on the different growth conditions. After the liquid culrures of Penicillium sp. KAC strain were filtrated, their antifungal activities against Rhizoctonia solani AG 1-1 and AG 2-2 were compared through growing Rhizoctonia solani AG 1-1 and AG 2-2 on potato dextrose agar amended with different culture filtrates. Depending on the media for the growth of Penicillium sp. KAC, the culture filtrate from potato dextrose broth showed the highest antifungal activity with inhibition efficiencies of 94.3% and 71.1% against Rhizoctonia solani AG 1-1 and AG 2-2, respectively. Depending on the growth periods of Penicillium sp. KAC, the culture filtrate from 7 day showed the highest antifungal activity with inghibition efficiencies of 100.0% and 100.0% against Rhizoctonia solani AG 1-1 and AG 2-2, respectively. Depending on the temperatures for the growth of Penicillium sp. KAC. the culture filtrate form 20℃ showed the highest antifungal activity with inhibition efficiencies of 92.9% and 94.2% against Rhizoctonia solani AG 1-1 and AG 2-2, respectively. Depending on the pH of the media for growth of Penicillium sp. KAC. the culture filtrate form pH 7.0 showed the highest antifungal activity with inhibition efficiencies of 85.7% and 94.2% against Rhizoctonia solani AG 1-1 and AG 2-2, respectively. Depending on the glucose contents of media for the growth of Penicillium sp. KAC, the culture filtrate form 30g of glucose content showed the highest antifungal activity with inhibition efficiencies of 69.6% and 43.5% against Rhizoctonia solani AG 1-1 and AG 2-2, respectively. However, there was no effect of nitrate contents amended in media used for the growth of Penicillium sp. KAC. on the inhibition of Rhizoctonia solani AG 1-1 and AG 2-2. The strain of Penicillium sp. KAC previously shown strong antifungal activity against Rhizoctonia solani AG 1-1 and AG 2-2 was cultured in liquid media on the different growth conditions. After the liquid cultures of Penicillium sp. KAC strain were filtrated, their antifungal activities against Rhizoctonia solani AG 1-1 and AG 2-2 were compared through growing Rhizoctonia solani AG 1-1 and AG 2-2 on potato dextrose agar amended with different culture filtrates. Depending on the media for the growth of Penicillium sp. KAC, the culture filtrate from potato dextrose broth showed the highest antifungal activity with inhibition efficiencies of 94.3% and 71.1% against Rhizoctonia solani AG 1-1 and AG 2-2, respectively. Depending on the growth periods of Penicillium sp. KAC, the culture filtrate from 7 day showed the highest antifungal activity with inhibition efficiencies of 100.0% and 100.0% against Rhizoctonia solani AG 1-1 and AG 2-2, respectively. Depending on the temperatures for the growth of Penicillium sp. KAC. the culture filtrate from 20℃ showed the highest antifungal activity with inhibition efficiencies of 92.9% and 94.2% aganist Rhizoctonia solani AG 1-1 and AG 2-2 , respectively. Depending on the pH of media for the growth of Penicillium sp. KAC, the culuture filtrate from pH 7.0 showed the highest. antifungal activity with inhibition efficiencies of 85.7% and 94.2% against Rhizoctonia solani AG 1-1 and AG 2-2, respectively. Depending on the glucose contents of media for the growth of Penicillium sp. KAC, the culture fi1trate from 3Og of glucose content showed the highest antifui1gal activity with inhibition efficiencies of 69.6% and 43.5% against Rhizoctonia solani AG 1-1 and AG 2-2, respectively. However, there was no effect of nitrate contents amended in media used for the growth of Penicillium sp. KAC on the inhibition of Rhizoctonia solani AG 1-1 and AG 2-2.