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인간태반에서 혈관내피성장인자 및 태반성장인자의 유전자 발현
신종철(Jong Chul Shin),이영(Young Lee),정대영(Dae Young Chung),백은정(Eun Jeong Baik),오민정(Min Jung Oh),양동은(Dong Eun Yang),김사진(Sa Jin Kim),김창이(Chang Ee Kim),김수평(Soo Pyung Kim) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.8
목적: 정상임신 및 비정상임신의 태반에서 혈관내피성장인자 및 태반성장인자의 유전자 발현유무 및 정도를 비교분석하기 위하여 시행하였다. 연구방법: 각 삼분기의 정상임신과 계류유산, 자궁내 태아발육지연 및 전자간증의 태반조직을 이용하여 총 RNA를 추출하였다. cDNA를 합성한 후 VEGF 및 PlGF primer를 사용하여 RT-PCR을 시행하였다. 결과: 정상임신과 계류유산의 모든 예에서 VEGF121, VEGF165 , VEGF189 등과 PlGF131, PlGF152 등이 확인되었으며, VEGF 및 PlGF 모두 임신주수에 따른 유전자 발현정도는 뚜렸한 차이를 보이지 않았다. 자궁내 태아발육지연에서는 4예 중 2예에서 4개의 VEGF 아형( VEGF121, VEGF145, VEGF165, VEGF189)이 모두 관찰되었다. 전간증에서는 3예 중 1예에서 5개의 VEGF아형이 모두 관찰되었다. 그러나 VEGF121, VEGF165, VEGF189 등과 PlGF131, PlGF152 등의 발현정도는 정상임신과 뚜렸한 차이를 보이지 않았다. 결론: VEGF 및 PlGF는 태반에서 발현될 뿐 아니라 VEGF의 경우는 자궁내 태아발육지연 및 중증 전자간증의 일부 예에서는 발현정도가 증폭되었다. 이러한 결과들은 VEGF가 정상임신 태반의 신생혈관생성에 관여하고 특히, 태아의 저산소증 상태에서는 VEGF의 생성이 증가할 가능성이 있음을 보여주고 있다. Objective : To determine whether gene expressions of VEGF and PlGF are different between the human placenta of normal and abnormal pregnancy. Methods : Placenta was collected at each trimester of normal pregnancy, missed abortion, intrauterine growth retardation and pre-eclampsia. Total RNA was extracted from placenta. Reverse transcription-polymerase chain reaction(RT-PCR) was performed using VEGF and PlGF primer. Results : VEGF121, VEGF165 and VEGF189 were identified in normal pregnancy and missed abortion. In two cases of four IUGR and one case of three pre-eclampsia, four of isoforms (VEGF121, VEGF145, VEGF165, and VEGF189) were identified. The intensity of signal was strongest for VEGF165 in all cases. PlGF131 and PlGF152 were identified in all cases. However, the signal intensities of VEGF121, VEGF165, VEGF189, PlGF131 and PlGF152 were not different according to the gestational age. They were also not different between normal pregnancy and abnormal pregnancy. Conclusion : VEGF and PlGF were not only expressed at placenta but also overexpressed in part of IUGR and pre-eclampsia. The results suggest that VEGF may play a role in the induction of angiogenesis of placenta in normal pregnancy and its production may be increased under the hypoxic condition.
간세포 성장인자가 HT 세포주에서 c - met 의 발현과 침투성에 미치는 영향
신종철(Jong Chul Shin),이지현(Jee Hyun Lee),양동은(Dong Eun Yang),오민정(Min Jeong Oh),문희봉(Hee Bong Moon),이영(Young Lee),김사진(Sa Jin Kim),이종승(Chong Seung Yi),김수평(Soo Pyung Kim) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.8
N/A Objective : To evaluate the invasive capacity of trophoblastic cells when treated with HGF, and see whether HGF induces c-met expression in vitro. Methods : HT cell line was treated with recombinant HGF (rHGF) at different concentrations (0, 10, 50 and 100 ng/mL) and cultured for 24 hours. To evaluate according different time of culture, HT cell line was pre-treated with 50 ng/mL rHGF and cultured for 24, 36, 48 and 72 hours. We analyzed the expression of c-met mRNA using reverse transcriptase-polymerase chain reaction and the expression of c-met protein using western blot in each samples. We also observed cellular invasion capacity through the invasion assay under a microscope and confirmed 72 kDa gelatinase and 92 kDa gelatinase expression patterns by zymography assay. Results : The expressions of c-met mRNA and protein were increased in all concentrations of rHGF, compared with that of normal control although it was not in dose-dependent fashion. In invasion assay, the number of invaded HT cells were increased in dose-dependent fashion, compared with that of normal control. In zymography ssay, the expression of 72 kDa gelatinase was increased in dose-dependent fashion, compared with the control. However, 92 kDa eglatinase was not detected in any studied group. Conclusion : These results suggests that HGF might be related to upregulation of trophoblast cell invasiveness by activation of c-met and subsequent induction of 72 kDa gelatinase.
신종철(Jong-Chul Shin),권순구(Soon-Koo Kweon),오이석(Yisok Oh),김세영(Se-Young Kim),전병태(Byeong-Tae Jeon) 한국전자파학회 2012 한국전자파학회논문지 Vol.23 No.6
위성 SAR(Synthetic Aperture Radar)를 정확하게 보정하기 위해서는 검보정에 사용되는 삼각 전파 반사기(Trihedral Corner Reflector: TCR)의 RCS(Radar Cross Section)를 정확하게 계산해야 한다. 본 연구에서는 TCR이 설치된 지표면이 TCR의 RCS 값에 얼마나 영향을 미치는지를 알아보기 위해서 지표면에서의 반사파와 TCR 모서리에서의 회절파를 이론적으로 계산하여 검보정 사이트의 지표면 상태에 따른 RCS 변화를 분석하였다. 지표면 반사파를 구하기 위해 지표면에 대한 PO 반사 계수를 이용하였으며, PO 반사 계수는 지표면 상태 변수인 거칠기와 유전율에 대한 함수이다. 중심 주파수 9.65 ㎓에서 지표면 위에 설치된 10 λ 크기 TCR의 RCS 값은 공기중의 TCR에 비해 0.46 ㏈ 변화가 발생하였고, 이 변화는 지표면의 상태 및 TCR 크기에 따라 크게는 1.55 ㏈ 정도의 차이가 날 수 있다. 지표면 영향에 따른 TCR의 RCS 값은 지표면의 거칠기가 작고 유전율이 클수록 크며, 중심 주파수가 낮고 TCR의 크기가 작을수록 크게 발생하였다. The radar cross section(RCS) of a trihedral corner reflector(TCR) should be accurately computed when it is used as an external calibration target for a satellite synthetic aperture radar(SAR) calibration campaign. This paper presents the RCS analysis on a trihedral corner reflector which is installed on a calibration site, using the wave reflection from the rough surface and the wave diffraction from the TCR edges. The results in this paper show quantitatively the effect of the front surface on the RCS of a TCR. The difference of the RCS between a TCR in air and a TCR on a ground surface is computed by including the interaction term which consists of the edge diffraction from the TCR edges and the surface reflection from the front rough surface. The reflection coefficient of a randomly rough surface is a function of the surface roughness and dielectric constant of the surface. The RCS of 10 λ size TCR on a ground is 0.46 ㏈ higher than TCR in air at 9.65 ㎓, and this can reach at maximum 1.55 ㏈ depending on a surface condition and TCR size. The effect of the front surface on the RCS of a TCR increases, as the surface roughness decreases, the soil moisture increases, and the size of TCR in wavelength decreases.
간세포 성장인자가 HT 세포주에서 matrix metalloproteinase 의 발현에 미치는 영향
신종철(Jong Chul Shin),문희봉(Hee Bong Moon),이지현(Jee Hyun Lee),양동은(Dong Eun Yang),이귀세라(Gui Se Ra Lee),이영(Young Lee),이종승(Jong Seong Lee),김창이(Chang Yi Kim),김수평(Soo Pyung Kim) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.12
N/A Objective : This study was performed to investigate the influence of hepatocyte growth factor (HGF) on matrix metalloproteinase (MMP), which are related in the lysis process of tissue during the invasion of trophoblasts. Method : HT cell line was treated with recombinant HGF (rHGF) of different concentration (0, 10, 50 and 100 ng/mL) and was cultured for 24 hours to check the changes in the expression of MMP-2 and MMP-9. Also, HT cell line was treated with recombinant HGF 50 ng/mL and was cultured for 24, 36, 48, and 72 hours to check the changes in the expression of MMPs according to the different time span. Total RNA were extracted from each cultured sample and RT-PCR and Western blotting were used to analyze the expression of MMP-2 and MMP-9. Results : MMP-2 mRNA expression with treated rHGF showed increase of 2, 2.5 and 2.2 times with the increase of concentration level of 10, 50 and 100 ng/mL accordingly, while MMP-2 protein expression were increased 1.4 and 1.5 times in 50 ng/mL and 100 ng/mL of rHGF respectively compared with that of normal control. MMP-9 mRNA showed no significant changes in its expression with all different levels of concentration, while MMP-9 protein showed 1.5 times increase with 10 ng/mL rHGF but 0.4 times decrease with 100 ng/mL. MMP-2 mRNA expression treated with recombinat HGF were increased 1.6 times with 24 hour culture and 2.3 times with 36 hour culture. MMP-2 protein showed 1.9 times increase only for the case of 24 hour culture. MMP-9 mRNA expression of recombinant HGF-treated groups was decreased 0.7 times compared with that of control group in 36 hours. MMP-9 protein expression were increased by 1.2, 1.6 and 1.9 times as culture time increase to 36, 48, and 72 hours accordingly, compared with that of normal control. Conclusion : This result suggests that the HGF might partially regulate the invasion of trophoblasts through MMP-2 and MMP-9.
3차원 초음파와 MRI를 이용하여 진단한 배꼽탈장을 동반한 흉제대쌍둥이
윤종숙 ( Jong Sook Yoon ),박미선 ( Mi Sun Park ),김민정 ( Min Jeong Kim ),이귀세라 ( Gui Se Ra Lee ),박인양 ( In Yang Park ),임현욱 ( Hyun Wook Lim ),신종철 ( Jong Chul Shin ),김사진 ( Sa Jin Kim ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.12
One of the most interesting congenital malformations is a conjoined twin. Conjoined twins are a rare occurrence in obstetric practice. More commonly known as Siamese twins, this phenomenon is shrouded in mystery and considered a curiosity by general public. Current technology is providing a basis for earlier diagnosis and a better prognosis. Frequently, the twins are born dead, but there are a few cases in which the twins survive. We present a case of thoraco-omphalophagus with omphalocele in 35 years old woman at 25weeks 5 days gestation by 3-D ultrasonography and MRI.
고추의 건조율 및 색도에 미치는 Blanching 효과
정신교(Shin-Kyo Chung),신종철(Jong-Chul Shin),최종욱(Jong-Uck Choi) 한국식품영양과학회 1992 한국식품영양과학회지 Vol.21 No.1
Blanching 처리가 고추의 건조율 및 색도에 미치는 효과를 조사하고자 실험실 규모의 열풍 건조기를 제작하였으며 strain gage형의 load cell과 strain amplifier를 이용하여 건조 중의 중량을 연속적으로 기록, 측정하였다. 고추의 건조특성곡선은 대조군에서는 예비, 항율, 감율건조기간이 뚜렷한 반면, blanching 처리군은 감율조건기간만 존재하였으며, 시료의 건조율과 건조상수 값은 blanching 처리 온도와 시간에 비례하여 증가하는 경향이었고, 100℃, 3분 blanching 처리군이 가장 높게 나타났다. Capsanthin 함량 역시 대조군에 비해 처리군이blanching 처리온도와 시간에 비례하였으며 색도 값의 경우 80℃, 3분 blanching군이 가장 우수하였다. blanching 처리는 전반적으로 고추의 건조율 증진 및 색도 보존에 효과적이며 80℃, 3분 blanching 처리가 비교적 적합하였다. To examine the effects of blanching treatments on the drying of hot red pepper, the pilot scale hot-air dryer equipped witb tbe weight sensor using strain gauge type load cell and strain amplifier was designed and manufactured, The drying characteristic curves of cut hot red pepper showed a settling down period followed by a constant rate period and falling rate period, but blanched hot red pepper showed only falling rate period. According as the blanching time and temperature rises, the drying rates and the capsanthin contents of hot red pepper fairly increased. Considering the drying rates and the color values of dried hot red pepper, we suggest the desirable blanching condition of hot red pepper should be water blanching at 80℃ for 3 mins.