YBR의 간섬유화(肝纖維化)억제 효과(效果)에 관한 연구(硏究)

승현석,우홍정,Seung, Hyun-Seok,Woo, Hong-Jung 대한한방내과학회 2010 大韓韓方內科學會誌 Vol.31 No.2

Objective : This study was performed to investigate the anti-fibrogenic effect and changes of inflammation-related genes by YBR I and YBR II (YBR I: Arteisiae Capillaris Herba, Atractylodis Rhizoma Alba, Hoelen/ YBR II: YBR I +Sanguisorbae Radix, Biotae Cacumen, Cirsii Japonici Herba) on HSC(hepatic stellate cells)-T6 and TAA-induced rat liver tissue. Materials and Methods : HSC-T6 were treated with various concentrations of distilled-water extract YBR I and YBR II extract for 24, 48 and 72 hours. After the treatment, cell viability, proliferation, procollagen levels and IL-6 levels were measured by using MTT Assay, BrdU Assay, Procollagen Type 1 C-peptide EIA kit, and Murine IL-6 ELISA Development kit. Rat liver fibrosis was induced by intraperitoneal TAA injection of 150mg/kg 3 times a week for 6 weeks. After the treatment, body weight, liver & spleen weights, liver function test, complete blood cell count and change of portal pressure were studied. In addition, gene expressions of ASMA, IL-6, MMP-2, TIMP-1 and TIMP-2, all of which are known to be associated with liver fibrosis, were analyzed by using Real-Time PCR. After YBR I and YBR IItreatment, percentages of collagen in TAA-induced rat liver tissue were measured. Results : The viability and proliferation of the HSC-T6 decreased as the concentration increased. The production of procollagen decreased as the concentration increased. The production of IL-6 was little influenced by YBR I and YBR II. There was no difference in rat body weight between the TAA-only group and the YBR groups. Compared with rat liver weight of TAA-only group, that of the YBR groups increased. In the YBR I group, the serum level of AST elevated by TAA injection significantly decreased and in the YBR I and II group, the serum level of ALP and ALT elevated by TAA injection decreased. In the YBR I group, white blood cell count elevated by TAA injection decreased but platelets increased. In the YBR I group, the portal pressure elevated by TAA injection significantly decreased. Decreases in the gene expression of ASMA and MMP-2 were observed in the YBR I group. The gene expression of IL-6 was little influenced by YBR I and YBR II -treated groups. In the histological finding, TAA injections caused severe fibrosis, but YBR I and YBR II treatment significantly reduced the amounts of hepatic collagens. Conclusions : These results suggest that YBR I and II have inhibitory effects on the hepatic fibrogenesis.

백선피(Dyctamnus dasycarpus)에 의한 약물유발성 간염 1예

승현석,류광렬,김영철,이장훈,우홍정,한창우,Seung Hyun-Suk,Rhew Kwang-Yul,Kim Young-Chul,Lee Jang-Hoon,Woo Hong-Jung,Han Chang-Woo 대한한방내과학회 2003 大韓韓方內科學會誌 Vol.24 No.2

Herbal remedies are enjoying a growing popularity in the world. But, we still pay few attention to the potential hepatotoxicity of herbal remedies, and there are only a few reports about that. We recently experienced a case of drug induced hepatitis caused by ingestion of Dyctamnus dasycarpus, one of herbs used for dermatologic disorders. The patient, a 38 year old woman, was admitted due to jaundice after ingestion of some herbal mixture containing Dyctamnus dasycarpus to treat her atopic dermatitis for 4 months. Other possible causes of liver injury were excluded by laboratory test and imaging studies, and we could find a report on similar cases caused by ingestion of Dyctamnus dasycarpus. During conservative treatment, elevated liver enzyme and total bilirubin level slowly returned to normal value. This case stresses the need to warn the potential hepatotoxicity of Dyctamnus dasycarpus, especially for a long time ingestion. So we suggest the necessity of toxicology screen at regular intervals during long time ingestion of herbal medicine, and further evaluation of the safety and toxicity of Dyctamnus dasycarpus.

인진청간탕(茵蔯淸肝湯)이 간보호(肝保護) 및 섬유화(纖維化) 억제(抑制)에 미치는 영향(影響)

승현석,이장훈,우홍정,김영철,Seung Hyun-Suk,Lee Jang-Hoon,Woo Hong-Jung,Kim Young-Chul 대한한방내과학회 2003 大韓韓方內科學會誌 Vol.24 No.1

Objective : The aim of this study is to investigate the inhibitory effect of Injinchunggantang on hepatic sclerosis induced by $CCl_4$. Methods : Weight, liver function test and complete blood cell count, gross findings, and findings on liver tissue of the past(Hematoxylin & Eosin stain, Masson-Trichrome stain) were studied. Results : When it comes to the change of rats' body weight, The $CCl_{4^+}$Injinchunggantang group lost far less weight than The $CCl_{4^-}$only group. In the liver function test, which is focused on various areas such as total cholesterol, alkaline phosphotase, albumin, aspartate transaminase, alanine transaminase, The $CCl_{4^+}$Injinchunggantang group was much more closer to normal limit than the $CCl_4$ only group. In the complete blood cell count, including white blood cell, red blood cell, hemoglobin, hematocrite, platelet, The $CCl_{4^+}$Injinchunggantang group significantly closer to normal limit than $CCl_{4^-}$only group. In the gross findings of hepatic fibrosis models, Injinchunggantang showed inhibitory effect on hepatic fibrosis in the order. In the past findings of hepatic fibrosis models in Hematoxylin & Eosin, Masson-Trichrome staining, the liver in $CCl_{4^-}$only group showed atrophy and necrotic change with white nodules, whereas that of $CCl_{4^+}$Injinchunggantang group showed lesser significant change with the well_preserved tone of the tissue. In the extent of the inhibition of the hepatic fibrosis, the Injinchunggantang group showed statistically significant inhibitory effect(p<0.05) in the sclerosis model. Conclusions : These results show that Injinchunggantang have inhibitory effect on hepatic sclerosis induced by $CCl_4$ and further ultimately prevent liver cirrhosis. To obtain more credible results in this experiment, the invention of a new experimental model more similar to human hepatic sclerosis is still needed.

茵蔯淸肝湯이 肝保護 및 纖維化 抑制에 미치는 影響

승현석,김영철,이장훈,우홍정 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2003 東西醫學硏究所 論文集 Vol.2003 No.-

Objective : The aim of this study is to investigate the inhibitory effect of Injinchunggantang on hepatic sclerosis induced by CCl₄. Methods : Weight, liver function test and complete blood cell count, gross findings, and findings on liver tissue of the past(Hematoxylin & Eosin stain, Masson-Trichrome stain) were studied. Results : When it comes to the change of rats body weight, The CCI₄-Injinchunggantang group lost far less weight than The CCl₄ only group. In the liver function test, which is focused on various area such as total cholesterol, alkaline phosphotase albumin, aspartate transaminase, alanine transaminase, The CCl₄ Injinchunggantang group was much more closer to normal limit than the CCl₄ only group. In the complete blood cell count, including while blood cell, red blood cell, hemoglobin, hematocrite, platelet, The CCl₄ Injinchunggantang group significantly closer to normal limit than CCl₄ only group. In the gross findings of hepatic fibrosis models, Injinchunggantang showed inhibitory effect on hepatic fibrosis in the order. In the past findings of hepatic fibrosis models in Hematoxylin & Eosin, Masson-Trichrome staining, the liver in CCl₄ only group showed atrophy and necrotic change with white nodules, whereas that of CCl₄ Injuchuinggantang group showed lesser significant change with the well preserved tone of the tissue. In the extent of the inhibition of the hepatic fibrosis, the Injinchunggantang group showed statistically significant inhibitory effect(p<0.05) in the sclerosis model. Conclusions : These results show that Injinchunggantang have inhibitory effect on hepatic sclerosis induced by CCl₄ and further ultimately prevent liver cirrhosis. To obtain more credible results in the experiment, the invention of a new experimental model more similar to human hepatic sclerosis is still needed.

피로를 호소하는 외래환자에 대한 임상적 관찰

박신명,승현석,김영철,이장훈,우홍정,이지현,Park, Shin-Myong,Seung, Hyun-Suk,Kim, Young-Chul,Lee, Jang-Hoon,Woo, Hong-Jung,Lee, Ji-Hyeon 대한한방내과학회 2001 大韓韓方內科學會誌 Vol.22 No.3

Objectives: Fatigue is a common symptom experienced by many people who visit Oriental medical clinics or hospital. However, there has been little study about the fatigue in the Oriental medical academic world. For this reason, we attempted to investigate the present status of fatigue of outpatients, and its relation with Health Practice Index(HPI). Methods: The subjects were 63 outpatients who visited the Tonification Clinic in Kyunghee Oriental Medical Center between January 1, 2001 and July 31, 2001. Their chief complaint was fatigue and they did not have any physical or mental problem. They were given a questionnaire which included questions reflecting general characteristics, fatigue degree and health habits. We measured degree of fatigue by Chalder scale et al. Health habits were investigated about 5 articles out of 'Breslow 7 Health habits'. Results: Among the subjects, 48 people(76.2%) were considered as 'fatigue patients' by the Chalder scale. Of this 48 fatigue patients, 27 people(56.3%) had manifested fatigue for more than 6 months. The average of scale II for all the patients was 14.05, which indicates moderate degree of fatigue. They complained fatigue, drowsiness and general weakness, dryness and discomfort of the eyes, headache, shoulder pain and neck stiffness, dizziness, heat in the upper part of the body, and poor concentration. There were no differences in degree of fatigue according to Health habits including exercise or not, smoking or not, the frequency of drinking, hours of sleeping, and body mass index. Conclusions: Many people complain fatigue symptom. Therefore doctors should have more interest in fatigue and care. This study can provide standards of prognosis of fatigue patients. Also prospective studies are needed to find relationship between health habits and fatigue degree.

가감생간탕 투여후 호전된 급성 간염 2예

박신명,한창우,김영철,이장훈,우홍정,승현석,Park, Shin-Myung,Han, Chang-Woo,Kim, Young-Chul,Lee, Jang-Hoon,Woo, Hong-Jung,Seung, Hyun-Suk 대한한방내과학회 2002 大韓韓方內科學會誌 Vol.23 No.2

Acute hepatitis is caused by virus, alcohol. toxins and drugs. Clinical symptoms of acute hepatitis are severe fatigue, jaundice, nausea, hepatomely, lymphadenopathy. We encountered two cases of acute hepatitis, one C type viral hepatitis and the other hepatitis by overuse of medicine. The symptoms of the latter are severe fatigue, petechia and heartburning. The symptoms of the latter are severe fatigue, nausea, dyspepsia, yellow urine and skin. We recognized that total bilirubin. direct bilirubin, serum transaminase, alkaline phosphatase and gamma glutamyl transferase were elevated. We treated both patients with herb medicine(Gagamsaenggan-tang). Gagamsaenggan-tang has been used to hepatic diseases and have been known to have beneficial effects. The patients' symptoms began to improve after about two weeks of treatment. After 3 weeks on medication, the clinical symptoms and liver function of two patients were improved.