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만성 B형 간염 바이러스 감염자에서 Enhancer 1과 Pre-X Promotor 부위의 염기 다양성
송우건 ( Woo Kern Song ),김학철 ( Haak Cheoul Kim ),박도심 ( Do Sim Park ),조지현 ( Ji Hyun Cho ) 대한소화기학회 2003 대한소화기학회지 Vol.41 No.3
Background/Aims: Hepatitis B Virus (HBV) is a compact DNA virus with four genes which are controlled by 6 promotors and two enhancers during its replication. However, HBV has variability in its nucleotide sequence and thus, it can be classified into genotypes. Recently, it is suggested that nucleotide mutations in its sequence are related to chronic liver disease (CLD). Therefore, we analyzed relationships between mutations and disease severity in its Enh 1 and X-promoter regions (1021-1373). Methods: HBV DNA of the patients with chronic hepatitis (CH, n=30), liver cirrhosis (LC, n=25), and hepatocellular carcinoma (HCC, n=17) was amplified and sequenced. Then, we constructed a phylogenetic tree (PAUP4.0) and analyzed their mutation rates and specificity among the disease groups. Results: All analyzed sequences belonged to genotype C. The divergency rate was 1.71% and there was no difference in their mutation rates, but there were some hot-spots among the disease groups. The hot-spots were at nucleotides 1317, 1229, 1126, 1134, and 1041 in decreasing order. Between the groups of LC and HCC, there were higher mutation rates at nucleotides 1126 (A→C) and 1134 (T→C) in LC, and at nucleotides 1229 (G→A) and 1317 (A→G) in HCC. The mutations at nucleotides 1126 and 1134 (p< 0.001) were accompanied. Conclusions: The genotype of HBV in Korea is type C in all cases and there might exist the associated mutations and hot-spots related to CLD severity in Enh 1 and X-promoter regions. (Korean J Gastroenterol 2003;41:211-218)
만성 B형 간염 바이러스 보유자에서 X-유전자 부위의 염기 변이
김학철,김지웅,송우건 대한소화기학회 2001 대한소화기학회지 Vol.37 No.4
Background/Aims: X-gene product of hepatitis B virus (HBV) is known to cis-/trans-activate a number of cellular and viral promotors. We studied the mutations of X gene and investigated the relationship between the mutations and the severity of disease in chronic HBV carriers. Methods: HBV DNA samples were obtained from the sera of 11 chronic asymptomatic carriers (ASC group), 16 chronic hepatitis patients (CH group), and 23 cirrhotic patients with HBV (LC group). PCR and direct sequencing analysis were performed for X gene. Results: LC group was older than ASC group and CH group. In X gene, there were many mutation points and 17 hot-spots were found. Their variability was 1.17%. The mutations were not found in the direct repeat (DR) and TATA box binding portion (TBP), but hot-spots were found frequently in the 2nd TATA-box area, negative regulatory element (lNRE) and C/EBP. The nucleotide mutations occurred frequently at 1762 and 1764 nucleotide. The associated mutations at 1762 and 1764 nucleotide were accompanied by the mutation at one of 1653, 1613 and 1631 nucleotide. Even in those accompanied mutation, there were not interlinked between them, but has trend to cross-link between them. Conclusions: DR and TBP portion in X gene might be an essential portion for HBV, and especially the 2nd TATA-box, NRE and C/EBP might be related to the severity of liver disease. Moreover there might be inter-, or cross-linked relationship between the mutations.
하부 위장관 출혈을 유발한 직장내 Dieulafoy양 병변
김태현,김상욱,최지훈,송우건,윤경호,김진아,최석채,나용호 圓光大學校 醫科學硏究所 1999 圓光醫科學 Vol.15 No.2
Dieulafoy's ulcer is an uncommon lesion that usually presents wih massive bleeding. Although it has been observed, for the most part, in the stomach, it has also been identified in the small bowel and colon A 35-year-old male presented with lower gastrointestinal bleeding, which was found to be caused by a Dieulafoy's ulcer in the rectum. Diagnosis was by colonoscopy. (This lesion was injected initially with endoscopic injection of a solution of hypertonic saline solution with 1/10,000 epinephrine followed by bipolar electrocoagulation.) No further bleeding has occurred with follow-up 6 months. We report one patients with extragastric Dieulafoy's lesion in the rectum.