고려대학교 VHIL 분자바이러스유전체 연구실

송문정 생화학분자생물학회 2012 생화학분자생물학회 소식 Vol.32 No.2

개념은유시각하적중한색채사대비연구(槪念隱喩視角下的中韓色彩詞對比硏究)-이(以)“백(白)”여(与)“희다”위중심(爲中心)

송문정 천진사범대학교 한국문화연구중심 2019 중한언어문화연구 Vol.16 No.-

This paper studied Chinese color word ‘baek(白)’and Korean color word ‘huida희다’ from the perspective of conceptual metaphor. The basic meanings of ‘baek(白)’and ‘huida 희다’ are almost the same. Although the positive and neutral extended meanings of‘baek (白)’are slightly less than that of ‘huida희다’, the negative extended meanings of ‘baek (白)’are much more than that of ‘huida희다’. Only a few of the extended meanings of the two can be one-to-one correspondence, and the remaining extended meanings of the two have multiple differences. Similar physiological basis, cognitive style and the influence of the Dongyang Chinese character culture circle are the basis for the commonality of the extended meanings of the two, and the different geographical and historical environment, ideology and religious beliefs lead to the extended meanings’ differences of the two. Understanding the characteristics and similarities and differences between Chinese and Korean white words will help to deepen understanding of the language and culture of the two countries and enhance cross-cultural exchanges.

“다중지능”이론 접목한 보드게임마당

송문정 장로회신학대학교 기독교교육연구원 2016 교육교회 Vol.451 No.-

A Gammaherpesvirus Establishes Persistent Infection in Neuroblastoma Cells

조혜정,송문정 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.7

Gammaherpesvirus (γHV) infection of the central nervous system (CNS) has been implicated in diverse neurological diseases, and murine γHV-68 (MHV-68) is known to persist in the brain after cerebral infection. The underlying molecular mechanisms of persistency of virus in the brain are poorly understood. Here, we characterized a unique pattern of MHV-68 persistent infection in neuroblastoma cells. On infection with MHV-68, both murine and human neuroblastoma cells expressed viral lytic proteins and produced virions. However, the infected cells survived productive infection and could be cultured for multiple passages without affecting their cellular growth. Latent infection as well as productive replication was established in these prolonged cultures, and lytic replication was further increased by treatment with lytic inducers. Our results provide a novel system to study persistent infection of γHVs in vitro following de novo infection and suggest application of MHV-68 as a potential gene transfer vector to the brain.

Dipeptidyl peptidase 4 (DPP4)/CD26 : Biological Functions, Disease Association, and Therapeutics

윤현이(Hyunyee Yoon),송문정(Moon Jung Song) 고려대학교 생명자원연구소 2021 생명자원연구 Vol.29 No.-

Dipeptidyl peptidase 4 (DPP4), 또는 CD26 단백질은 세포막 바깥쪽 도메인에 단백질의 2번 위치에 존재하는 프롤린이나 알라닌 잔기를 절단하는 dipeptidyl peptidase 효소활성을 갖는 다기능 당단백질이다. 세포내 중요한 신호전달 인자로 작용하는 다양한 생리활성 물질들이 DPP4의 기질이기 때문에, DPP4는 당대사 조절, 항염증반응 및 심혈관대사 작용에 중요한 역할을 한다고 알려져 있다. 흥미롭게도 고병원성 사람코로나바이러스인 MERS-CoV의 세포수용체로서 DPP4의 역할도 보고되었다. DPP4는 관련된 많은 질환에서 중요한 약물 표적으로 여겨져 왔기 때문에, 본 논문에서는 DPP4의 생물학적 기능, 질병연관성, 치료제 등에 관한 내용을 살펴보고자 한다. Dipeptidyl peptidase 4 (DPP4; also known as CD26) is a multifunctional glycoprotein containing an ecto-domain with didpetidyl peptidase enzymatic activity that cleave the proteins containing a position 2 proline or alanine. Since there are a variety of bioactive DPP4 substrates acting as important signaling messengers, DPP4/CD26 is shown to play key roles in transducing the glucoregulatory, anti-inflammatory, and cardiometablolic actions. Moreover, DPP4 can function through protein-protein interactions to exert its biological functions, regardless of its enzymatic activity. Interestingly, DPP4 was identified as a host receptor for middle east respiratory syndrome coronavirus (MERS-CoV), a highly pathogenic human coronavirus. Since DPP4 has been a critical therapeutic target for many related diseases, in this review, we will discuss the current understanding of DPP4/CD26 regarding its biological functions, disease association, and therapeutic approaches.

Antiviral effects of G-quadruplex binding ligands against human herpesviruses

한지호(Ji Ho Han),송문정(Moon Jung Song) 고려대학교 생명자원연구소 2022 생명자원연구 Vol.30 No.-

G-quadruplexes(G4s)는 복합체를 형성하는 구아닌이 풍부한 평면 모양의 tetrad 형성의 스택으로 구성된 고전적인 이차 핵산 구조이다. G4는 주요 세포 과정의 전사, 번역, 텔로미어 유지, 후성유전적 조절, 복제 및 재조합에서 다양한 중요한 역할에 연루되어 있다. G-quadruplexes는 종양학에서 중요한 구조로 처음 발견되었지만 지난 10년 동안 다양한 바이러스와의 관련성이 더욱 분명해 지고 있다. 인간 허피스바이러스는 허피스바이러스과(Family)에 속하는 DNA 바이러스이며 숙주에서 용해성 및 잠복성 감염이라는 두 가지 유형의 감염 생활사를 갖는 것이 특징적이다. 잠복기 동안 바이러스는 평생 잠복 상태를 유지하고 간헐적으로 재활성화되어 바이러스가 숙주내, 숙주간 전파할 수 있도록 하고, 한편 숙주에서 질병 등의 문제를 야기할 수 있다. 최근에 바이러스 게놈에서 G4s의 역할과 G4에 결합하는 G4 리간드의 잠재적인 항바이러스 효능에 관한 보고가 증가하고 있다. 많은 연구 결과들이 바이러스 G4s가 바이러스 생활 주기에서 중요한 역할을 하고, G4 리간드의 치료가 용해성 및 잠복성 감염 모두에서 항바이러스 활성을 나타낼 수 있다는 것을 시사한다. 이 리뷰에서는 허피스바이러스 게놈에서 G4s의 중요성과 이러한 기전을 연구하는데 사용되는 강력한 G4 결합 리간드를 함께 소개하고, 마지막으로 각 G4 리간드의 고유한 기능적 특성을 설명하고자 한다. G-quadruplexes (G4s) are noncanonical secondary nucleic acid structures constituted by stacking of guanine rich planar shaped tetrad formations that form a complex. G4s are implicated for various important roles in key cellular processes transcription, translation, telomere maintenance, epigenetic regulation, replication, and recombination. G-quadruplexes were first discovered as important structures in oncology, but for the past decade its relevance in viruses is becoming more evident. Human herpesviruses are DNA viruses of the Herpesviridae family and are unique in characteristic with two types of infection which can be distinguished by lytic and latency establishment in the host. During latency the virus maintains lifelong dormancy and intermittently undergoes reactivation, causing the host medical problems. Recently there are increasing number of reports regarding role of G4s in viral genomes and the potential antiviral efficacy of G4 ligands, including G4s in latency. Many results suggest viral G4s play significant roles in the virus life cycle and treatment of G4 ligands exhibit antiviral activities in both lytic and latent infections. In this review, the importance of G4s in herpesvirus genomes will be introduced with the potent G4 ligands used to study these mechanisms and finally explain the distinct functional properties of each G4 ligands.

Analysis of IE62 mutations found in Varicella-Zoster virus vaccine strains for transactivation activity

고혜민,이광명,신옥,송문정,이찬희,김영의,안진현 한국미생물학회 2018 The journal of microbiology Vol.56 No.6

Live attenuated vaccine strains have been developed for Varicella- Zoster virus (VZV). Compared to clinically isolated strains, the vaccine strains contain several non-synonymous mutations in open reading frames (ORFs) 0, 6, 31, 39, 55, 62, and 64. In particular, ORF62, encoding an immediate-early (IE) 62 protein that acts as a transactivator for viral gene expression, contains six non-synonymous mutations, but whether these mutations affect transactivation activity of IE62 is not understood. In this study, we investigated the role of non-synonymous vaccine-type mutations (M99T, S628G, R958G, V1197A, I1260V, and L1275S) of IE62 in Suduvax, a vaccine strain isolated in Korea, for transactivation activity. In reporter assays, Suduvax IE62 showed 2- to 4-fold lower transactivation activity toward ORF4, ORF28, ORF29, and ORF68 promoters than wild-type IE62. Introduction of individual M99T, S628G, R958G, or V1197A/ I1260V/L1275S mutations into wild-type IE62 did not affect transactivation activity. However, the combination of M99T within the N-terminal Sp transcription factor binding region and V1197A/I1260V/L1275S within the C-terminal serineenriched acidic domain (SEAD) significantly reduced the transactivation activity of IE62. The M99T/V1197A/I1260V/ L1275S mutant IE62 did not show considerable alterations in intracellular distribution and Sp3 binding compared to wild-type IE62, suggesting that other alteration(s) may be responsible for the reduced transactivation activity. Collectively, our results suggest that acquisition of mutations in both Met 99 and the SEAD of IE62 is responsible for the reduced transactivation activity found in IE62 of the VZV vaccine strains and contributes to attenuation of the virus.

종양유발 인체 감마허피스바이러스를 제어하는 천연 식물화합물의 항바이러스 작용

정우창(Woo-Chang Cheong),송문정(Moon Jung Song) 고려대학교 생명자원연구소 2017 생명자원연구 Vol.25 No.-

Abstract Gammaherpesviruses are a subtype of the Herpesviridae family. They undergo lytic replication in epithelial cells and establish latency in lymphocytes, leading to persistent infection in the host. Human gammaherpesviruses are called oncogenic viruses, as their infections are associated with various types of cancers. Therefore, the control of human gammaherpesviruses is important for the treatment of virus-associated cancers and inhibition of virus infection in the host. Although there are antiviral treatments against human gammaherpesviruses, toxicity issues and the emergence of drug resistance are limitations to their use. To overcome these issues, studies are focusing on identifying alternative treatments, such as natural phytochemical products with antiviral activity against gammaherpesviruses. In this paper, we review the characteristic features of two human gammaherpesviruses, Epstein-Barr virus (EBV) and Kaposi’s sarcoma-associated herpesvirus (KSHV), and their related diseases. The current state of research on natural phytochemicals with anti-gammaherpesvirus activity is then discussed. Finally, we further discuss the limitations and prospects of anti-gammaherpesvirus research.

Antiviral activity of Schizonepeta tenuifolia Briquet against noroviruses via induction of antiviral interferons

Yee Ching Ng,김예원,이정수,이성준,송문정 한국미생물학회 2018 The journal of microbiology Vol.56 No.9

Human noroviruses are the causative agents of non-bacterial gastroenteritis worldwide. The rapid onset and resolution of disease symptoms suggest that innate immune responses are critical for controlling norovirus infection; however, no effective antivirals are yet available. The present study was conducted to examine the antiviral activities of Schizonepeta tenuifolia Briquet extract (STE) against noroviruses. Treatment of human norovirus replicon-bearing HG23 cells with STE at 5 and 10 mg/ml concentrations resulted in the reduction in the viral RNA levels by 77.2% and 85.9%, respectively. STE had no cytotoxic effects on HG23 cells. Treatment of RAW 264.7 cells infected with murine norovirus 1 (MNV-1), a surrogate virus of human noroviruses, with STE at 10 and 20 μg/ml concentrations resulted in the reduction of viral replication by 58.5% and 84.9%, respectively. STE treatment induced the expression of mRNAs for type I and type II interferons in HG23 cells and upregulated the transcription of interferon-β in infected RAW 264.7 cells via increased phosphorylation of interferon regulatory factor 3, a critical transcription regulator for type I interferon production. These results suggest that STE inhibits norovirus replication through the induction of antiviral interferon production during virus replication and may serve as a candidate antiviral substance for treatment against noroviruses.

Development of a neutralization assay based on the pseudotyped chikungunya virus of a Korean isolate

정우창,Kwang Yeon Hwang,Suk-Jo Kang,김재욱,송문정 한국미생물학회 2020 The journal of microbiology Vol.58 No.1

The Chikungunya virus (CHIKV) belongs to the Alphavirus genus of Togaviridae family and contains a positive-sense single stranded RNA genome. Infection by this virus mainly causes sudden high fever, rashes, headache, and severe joint pain that can last for several months or years. CHIKV, a mosquito- borne arbovirus, is considered a re-emerging pathogen that has become one of the most pressing global health concerns due to a rapid increase in epidemics. Because handling of CHIKV is restricted to Biosafety Level 3 (BSL-3) facilities, the evaluation of prophylactic vaccines or antivirals has been substantially hampered. In this study, we first identified the whole structural polyprotein sequence of a CHIKV strain isolated in South Korea (KNIH/2009/77). Phylogenetic analysis showed that this sequence clustered within the East/ Central/South African CHIKV genotype. Using this sequence information, we constructed a CHIKV-pseudotyped lentivirus expressing the structural polyprotein of the Korean CHIKV isolate (CHIKVpseudo) and dual reporter genes of green fluorescence protein and luciferase. We then developed a pseudovirus-based neutralization assay (PBNA) using CHIKVpseudo. Results from this assay compared to those from the conventional plaque reduction neutralization test showed that our PBNA was a reliable and rapid method to evaluate the efficacy of neutralizing antibodies. More importantly, the neutralizing activities of human sera from CHIKVinfected individuals were quantitated by PBNA using CHIKVpseudo. Taken together, these results suggest that our PBNA for CHIKV may serve as a useful and safe method for testing the neutralizing activity of antibodies against CHIKV in BSL-2 facilities.