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Autographa californica 핵다각체병 바이러스의 IE1 유전자에 의해 형질전환된 곤충세포주의 특성
조은숙,박혜진,이광식,강석우,윤은영,김근영,손흥대,진병래,Cho, Eun-Sook,Park, Hae-Jin,Lee, Kwang-Sik,Kang, Seok-Woo,Yun, Eun-Young,Kim, Keun-Young,Sohn, Hung-Dae,Jin, Byung-Rae 대한미생물학회 1999 Journal of Bacteriology and Virology Vol.28 No.1
Transformation efficiency, virus multiplication and foreign gene expression were characterized in the insect cells transformed with Autographa californica nuclear polyhedrosis virus (AcNPV) immediate early 1 gene (IE1). Transformation efficiency of insect cells by AcNPV IE1 gene vector horboring foreign gene was approximately 8-fold higher in the Sf9 cells transformed previously with AcNPV IE1 gene than in the normal Sf9 cells. Virus multiplication and foreign gene expression of recombinant baculovirus in the Sf9 cells transformed with AcNPV IE1 gene were similar to those of the normal Sf9 cells. These results suggest that transformed cells displaying foreign gene product by using AcNPV IE1 gene promoter will be useful for the diverse applications of insect cells.
A stydy on The Biological Control of Sciarid Fly(Lycoriella sp.) Using Bacillus thuringiensis
최광호,박현철,박현우,진병래,강석권,손흥대,Choi, Kwang-Ho,Park, Hyean-Cheal,Park, Hyun-Woo,Jin, Byung-Rae,Kang, Seok-Kwon,Sohn, Hung-Dae 한국생명과학회 1996 생명과학회지 Vol.6 No.4
Thirteen subspecies of Bacillus thuringiensis including B. t. israelensis, B. t. morrisoni PG-14 and B.t. darmstadoemsos known to be toxic to dipteran insects were treated on the mushroom (Flammulina velutipes) compost to estimate the biological control effect of a sciarid fly, Lycoriella sp. According to the results, it was found that there were no significant effects of the tested strains of B, thuringiensis on the control of Lycoriella sp. For further confirmation, larval gut juice of Lycoriella sp. and trypsin were respectively treated into the parasporal crystal proteins of three subspecies of B. t. israelensis, B. t. morrisoni PG-14, and B. t. darmstadiensis. The proteins were separated by SDS-PAGE. According to the results, the major parasporal crystal proteins were respectively produced by B. t. morrisoni as the amount of 52 kd, B. y. israelensis as 37kd and B. t. darmstadiensis as 39kd, but the activity of these proteins could not be unfortunately confirmed in this study.
Microsatellite DNA 를 이용한 진돗개 ( Canis familiaris ) 집단의 유전적 구조에 관한 연구
윤신근(Sin Keun Youn),김익수(Ik Soo Kim),손흥대(Hung Dae Sohn) 한국유전학회 2001 Genes & Genomics Vol.23 No.1
N/A Characteristics of population genetic structure of the Jindo dog breed inhabiting Korean mainland and the offshore islet, Jindo, where the name of the dog originated, were compared using four microsatellite DNA loci. Considerably higher genetic diversity estimates and allele numbers were observed in the Jindo islet population, indicating genetic heterogeneity in this population. This is quite different from what one can expect because currently much less numbers of Jindo dogs are raised in Jindo islet (∼10,000 in Jindo islet vs. ∼100,000 in mainland). We discussed the observed result in terms of non-random breeding in the mainland population and possible release of originally pure dogs to mainland. Although the mainland population showed a significant deviation from Hardy-Weinberg equilibrium, higher homozygosity, and highly positive FIS in two loci, the Jindo islet population revealed these phenomena only in one locus, suggesting that Jindo dogs in mainland propagate more severely through small numbers of dogs. This non-random breeding also appears to be responsible for the differentiated gene pool between the two populations.
누에 유충의 혈림프 유약호르몬 에스테라제 활성의 조절에 關한 硏究
孫興大,姜弼敦 한국잠사학회 1992 한국잠사곤충학회지 Vol.34 No.1
Effects of starvation, ligation, refeeding and methoprene treatment in the feeding phase of the fifth instar lavae of Bombyx mori on the regulation of juvenile hormone esterase(JHE) activity were investigated. Starvation and ligation contributed to the reduction of JHE activity, however. JHE levels in starved lavae were slightly higher than in ligated larvae. Haemolymph JHE activity of starved larvae was increased by refeeding, and duration of increasing time of JHE activity after starvation was related to duration of starvation. When starved larvae were applied methoprene topically. JHE activity were not changed at day 0 and 1, but were increased by 1.3~1.4 times between day 2 and 5. When ligated larvae were applied methoprene topically, JHE activity was not changed at day 0, but were increased by 1.9~2.3 times between day 1 and 5. These results suggest that head factor, juvenile hormone(JH) and nutrient are major factors in the regulation of JHE in the feeding phase of the fifth instar larvae of Bombyx mori. Especially JHE might be regulated by the co-operative action of head factor and JH. However, head factor plays important role in the early stage, while JH plays important role thereafter.
幼若홀몬이 家蠶의 體液蛋白質 및 아미노酸의 變動에 미치는 影響
孫興大 한국잠사학회 1986 한국잠사곤충학회지 Vol.28 No.1
The experiment was carried out to investigate the effects of juvenile hormone analogue on changes of protein and amino acids in haemolymph of silkworm larva. Juvenile hormone analogue was topically administered to larvae at dose of 1 μg and 10μg Per gm of body weight at 60hr. of the 5th instar. The results obtained were as follows; 1. Larval duration of the fifth instar was extended about 1 day by JHA-1 and 4 days by JHA-10εs compared with the control. 2. Cocoon weight and cocoon layer weight by topical application of JHA were heavier than those of the control, but cocoon layer ratio was decreased in JHA-10 to the exclusion of JHA-1. 3. The concentration of haemolymph protein during the fifth instar was increased remarkably by the JHA application. 4. The total content of amino acids in haemolymph proteins of JHA-10 approximately doubled that of the control, with the conspicuous increase of glycine and arginine level.
SDS-Polyacrylamide gel 電氣泳動에 의한 누에의 發育段階別 血蛋白質의 變化
孫興大 건국대학교 1986 論文集 Vol.22 No.1
This experiments were carried out to study changes of haemolymph proteins during development of silkworm, Bombyx mori by SDS-polyacrylamide gel electrophoresis. The results obtained were follows; 1. Bands of haemolymph proteins seperated during the metamorphosis of silkworm showed 20 bands and those molecular weights ranged from 12,800 to 106,000 daltons. 2. Major bands (7 and 15) were changed quantitatively, band 7 showed predominant levels from the middle 5th instar to the middle pupa stage, band 15 was leached the maximum levels at spining and then significantly decreased on prepupa stage, those molecular weights were determined to be 27,000 and 79,000 daltons respectively. 3. Female specific proteins of band 17 and 18 (mol. wt. 102,000 and 102,500 daltons) showed from the 1st day after pupation. 4. Concentration of low molecular weight proteins (mol. wt. 16,300∼18,500 daltons) at adult stage were increased. 5. In conclusion, it was suggested that the major haemolymph proteins in silkworm should be utilized to form tissues of pupa and adult, female specific proteins involved in the egg formation.
누에 體液의 幼若호르몬 結合蛋白質(Juvenile hormone binding protein)에 관한 硏究
孫興大 한국잠사학회 1988 한국잠사곤충학회지 Vol.30 No.1
In order to examine a physiological role of juvenile(JH) binding proteins in the hemelymph of the silkworm larva, Bombyx mori, [3H] JH I incubated hemolymph was separated by polyacrylamide gel electrophoresis in the fifth-instar larva and the activity of the binding protein was analyzed using charcoal binding assay. The results obtained were as follows; 1. The JH was bound by two protein fractions in the hemolymph of the fifth-instar larva; One was JH binding lipoprotein(JH-LP), thr other was JH specific binding protein (JHBP). Their relative mobility values (Rm) were 0.3~0.33 and 0.81~0.84, respectively. There were no valid differences in those values from developmental stages of both male and female silltworms. 2. Total protein contents of the hemolymph were gradually increased during the fifth-instar larva, while at the prepupa decreasd. The maximum ones were observed at the spinning period and the contents from female were much higher than those from the male. 3. JH binding activity per ml of the hemolymph was low in the early stage of the fifth-instar larva and its activity was maximized at the spinning period and at the prepupa slightly decreased. 4. There was a similar pattern between changes of the JH binding activity per ml of the homolymph and of the total protein contents of the hemolymph. 5. The JH binding activity per mg of the hemolymph proteins was high in the early stage of the fifth-instar larva, while from the 6th day of the fifth-instar larva to the prepupa its activity showed the lowest levels.