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무인항공기의 비행 중 박리 측정을 위한 벽면 전단 응력 센서의 제작 및 성능검증
서대반,배남호,권세진 한국항공우주학회 2012 한국항공우주학회 학술발표회 논문집 Vol.2012 No.11
이번 연구에서는 무인 공격기에 적용되어 경계층 제어를 위한 박리 발생 정보를 제공해줄 벽면 전단 응력 센서의 개발을 위해, 이에 대한 첫 번째 연구로 스트레인 게이지가 적용된 초소형 벽면 전단 응력 센서의 개념을 제안하고 센서의 설계 과정을 수행하였다. 제작 공정을 설계하고 공정 진행을 통해 제안된 센서를 제작을 완료하였다. 제작된 센서의 온도에 대한 민감도 평가를 통하여 매우 낮은 TCR 값을 가짐을 확인하였다. 이번 연구에서 제안된 센서 개념을 검증하기 위하여 유속에 따른 센서의 저항 변화를 풍동 실험을 통해 측정하였고, 박리 발생 측정 가능성을 검증하기 위하여 에어포일 섹션을 이용하여 실험을 수행하였다. 실험 결과 이번 연구에서 제안된 센서의 개념의 가능성을 확인하였으며, 박리 발생 측정 가능성도 확인되었다. The new type of the floating element-based micro shear stress sensor was proposed for detection of the separation over a UCAV-wing. The strain on the tethers generated by the wall shear stress is measured using strain gauges. The first version of this type of the sensor was designed and fabricated to verify the concept. The TCR was evaluated, and resistance change of the strain gauge according to wind velocity was measured by wind tunnel test. Through the test, the proposed concept was verified. The separation-detection test was conducted using an airfoil section. The resistance of the sensor was measured as increasing angle of attack. Based on this result, the possibility of separation detection was verified.
흑연 및 Mo 기판 위에 CVD 성장된 Re 코팅층의 미세조직
이선규,남동윤,배남호,임성택,오용준 대한금속·재료학회 2018 대한금속·재료학회지 Vol.56 No.3
Rhenium coatings were fabricated on a graphite substrate at 1100 and 1250 oC and a molybdenum substrate at 1150 and 1250 oC by chemical vapor deposition (CVD) using ReCl5 precursor, and the effects of deposition temperatures and substrates on microstructure and crystal orientation were investigated. The rhenium coating on graphite infiltrated the pores about hundred micrometers into the graphite, resulting in a large contact area between the coating and the graphite. However, the rhenium coating on molybdenum showed inferior adhesion, introducing voids and cracks in the Mo metal due to additional reactions between the precursor and Mo. As deposition temperature increased, rhenium grains on the graphite increasingly developed a preferred (0002) orientation normal to the coating, while on molybdenum they tended to develop other orientations, such as pyramidal and prism planes. These behaviors were discussed in relation to interfacial microstructure.
Droplet-based Digital PCR System for Detection of Single-cell Level of Foodborne Pathogens
장민정,정순우,배남호,송윤성,이태재,이문근,이석재,이경균 한국바이오칩학회 2017 BioChip Journal Vol.11 No.4
Recently, foodborne pathogen is a common and distressing disease around world to cause a threat to life and economic damages and it is urgent to develop a tools to diagnosis of such pathogens in the early stage to prevent potential outbreak. Although conventional cell extraction and recovery of DNA from pathogen and PCR method have been widely used, the methods require complex steps, experts, and expensive chemicals and instruments to improve the PCR performance. Herein, we report a droplet-based polymerase chain reaction (ddPCR) system which allows identifying single-cell level of foodborne pathogens. E. coli O157:H7 and Salmonella cells were selected as model bacterial foodborne pathogens. The ddPCR system could be a useful platform for the quantitative detection of foodborne pathogens without any pretreatment process.
최윤석 ( Yoon-suk Choe ),배남호 ( Nam-ho Bae ),이찬구 ( Chan-goo Lee ),김도용 ( Do-yong Kim ) 한국환경기술학회 2018 한국환경기술학회지 Vol.19 No.5
본 연구에서는 기압 소자의 연구개발을 통해 수입에만 의존하던 기상 관측을 위한 정밀 기압계를 국내 기술로 만들려고 노력했다. 압저항 기압 센서의 소자는 나노기술을 활용한 MEMS 공정을 사용하여 제작하였다. 소자의 최소 선폭은 1 ㎛, 센서 선폭은 5 ㎛이다. 기압 센서는 저항 시험을 통해각 bridge 사이의 저항이 일정하다는 것이 확인되었다. 압력이 500 hPa에서 1100 hPa로 증가 할 때, 저항의 변화율은 5 Ω이었다. 그것은 기존의 외국 센서 제품에 근접하였다. 기압계는 개발된 기압소자를 기반으로 설계하여 제작하였다. 기압 측정 시험 결과, ±0.4 hPa(±0.5 hPa) 이하의 오차가 확인되었다(국내 기상기구 보정 허용 오차). 그러나 에폭시 접착 문제로 인해 시간이 지남에 따라 오차가 커지는 문제가 발생하여 향후 연구를 통해 이를 보완할 필요가 있다. In this paper, we tried to make precision barometer for meteorological observations, which was only dependent on imports with domestic technology through research and development of barometric pressure sensor. Piezoresistive pressure sensors were fabricated using MEMS process. Minimum line width is 1 ㎛, sensor line width is 5 ㎛. Through the resistance test, it was confirmed that the resistance between each bridge is uniform. When the pressure was increased from 500 hPa to 1100 hPa, the change rate of resistance was 5 Ω. It was close to that of the existing foreign sensor products. Barometer was designed and fabricated based on the barometric pressure sensor manufactured. As a result of the air pressure measurement test, it was confirmed that an error of ±0.4 hPa lower than ±0.5 hPa (Domestic meteorological instrument calibration tolerance). However, due to the epoxy adhesion problem, there is a problem that the error becomes large over time, and it is necessary to complement this part through further studies.
Flexible nanopillar-based electrochemical sensors for genetic detection of foodborne pathogens
Park Yoo Min,임선영,정순우,Song Younseong,배남호,홍석복,최봉길,이석재,이경균 나노기술연구협의회 2018 Nano Convergence Vol.5 No.15
Flexible and highly ordered nanopillar arrayed electrodes have brought great interest for many electrochemical applications, especially to the biosensors, because of its unique mechanical and topological properties. Herein, we report an advanced method to fabricate highly ordered nanopillar electrodes produced by soft-/photo-lithography and metal evaporation. The highly ordered nanopillar array exhibited the superior electrochemical and mechanical properties in regard with the wide space to response with electrolytes, enabling the sensitive analysis. As-prepared gold and silver electrodes on nanopillar arrays exhibit great and stable electrochemical performance to detect the amplified gene from foodborne pathogen of Escherichia coli O157:H7. Additionally, lightweight, flexible, and USB-connectable nanopillar-based electrochemical sensor platform improves the connectivity, portability, and sensitivity. Moreover, we successfully confirm the performance of genetic analysis using real food, specially designed intercalator, and amplified gene from foodborne pathogens with high reproducibility (6% standard deviation) and sensitivity (10 × 1.01 CFU) within 25 s based on the square wave voltammetry principle. This study confirmed excellent mechanical and chemical characteristics of nanopillar electrodes have a great and considerable electrochemical activity to apply as genetic biosensor platform in the fields of point-of-care testing (POCT).
Micro-injection Molded Droplet Generation System for Digital PCR Application
조다애,김소영,강현우,표성한,정남규,배남호,이석재,김용태,이경균 한국바이오칩학회 2022 BioChip Journal Vol.16 No.4
Sensitive, effective, and quantitative analysis of infectious pathogens is an important task for the prevention of human health threats. Herein, we present an advanced approach to producing gene-encapsulated microdroplets for quantitative analysis using a micropatterned metal mold and injection molding technique with an automatically operated system. An injection molded microdroplet generation device was successfully fabricated with a minimum channel width of 30 μm and optimized to produce 100 μm diameter droplets. The optimized microchannel design and flow rate also enable the production of stable numbers of microdroplets (~ 16, 000 droplets). To verify the applicability of our device and system to droplet-based digital PCR analysis, Escherichia coli ( E. coli ) O157:H7 was selected as a model bacterial pathogen, and the stx2 gene was amplified in the microdroplets. The generated microdroplets exhibit both chemical and mechanical stability, and our results are similar to those obtained by a commercially available method. Accordingly, the usefulness of the microdroplet generative device and system is confirmed as a simple, fast, and reliable tool for the quantitative molecular analysis of infectious diseases.
Kim Kyung Hoon,양민호,Song Younseong,Kim Chi Hyun,정영미,배남호,장성진,이석재,Kim Yong Tae,최봉길,이경균 나노기술연구협의회 2021 Nano Convergence Vol.8 No.30
A bacteria-capturing platform is a critical function of accurate, quantitative, and sensitive identification of bacterial pathogens for potential usage in the detection of foodborne diseases. Despite the development of various nanostructures and their surface chemical modification strategies, relative to the principal physical contact propagation of bacterial infections, mechanically robust and nanostructured platforms that are available to capture bacteria remain a significant problem. Here, a three-dimensional (3D) hierarchically structured polyaniline nanoweb film is developed for the efficient capture of bacterial pathogens by hand-touching. This unique nanostructure ensures sufficient mechanical resistance when exposed to compression and shear forces and facilitates the 3D interfacial interactions between bacterial extracellular organelles and polyaniline surfaces. The bacterial pathogens ( Escherichia coli O157:H7, Salmonella enteritidis , and Staphylococcus aureus ) are efficiently captured through finger-touching, as verified by the polymerase chain reaction (PCR) analysis. Moreover, the real-time PCR results of finger-touched cells on a 3D nanoweb film show a highly sensitive detection of bacteria, which is similar to those of the real-time PCR using cultured cells without the capturing step without any interfering of fluorescence signal and structural deformation during thermal cycling. Graphic Abstract
우수한 On/Off 저항비와 내구성을 가지는 Si CMOS-호환형 ReRAM 개발 및 전기-화학 바이오-트랜스듀서 활용
권윤아(Youna Kwon),김영준(Young-Joon Kim),심갑섭(Gapseop Sim),유동은(Dongeun Yoo),배남호(Nam Ho Bae),이경균(Kyoung Gyun Lee),안재혁(Jae-Hyuk Ahn),이종원(Jongwon Lee) 대한전자공학회 2023 대한전자공학회 학술대회 Vol.2023 No.6
In this study, ReRAM devices for electro-chemical bio applications were developed, and a biotransducer with a threshold-sensing function using the ReRAM was developed for the first time. The fabricated Si CMOS-compatible ReRAM device exhibited an excellent on/off resistance ratio of 50 with high endurance compared to the previous results. The threshold-sensing function of the implemented bio-transducer was confirmed experimentally as intended for various pHs of 0 to 10. It was also possible to change the threshold point utilizing a voltage variable function in the circuit. It is expected that this technology can be used into high-density/low-power edge-type biosensors for various infectious diseases.