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박표잠,남경수,김세권 한국생명과학회 1998 한국생명과학회 학술발표회 Vol.20 No.-
Egg yolk protein(EYP) has been used commercially because it contains a lot of lecithins which are used as emulsifying agents in food and medicine. Recently, the antioxidative activities of egg yolk phospholipids and ovalbumin by heat treatment have been reported. However, the research for lecithin-free egg yolk protein as functional material has not approached. In this study, EYP was investigated as the antioxidant. EYP was hydolyzed with alcalase, and the hydrolysate was separated into three major types, EYP I , EYPII, and EYPIII, according to their relative molecular weights. In addition, the antioxidative effects of the hydrolysates were studied on lipid peroxidation and antioxidative enzyme activites in tert-butyl hydroperoxide(t-BHP). The hydrolysates of EYP were not toxic and increased the growth of normal human liver cell(Chang). The hydrolysates increased the viability of liver cell after treatment with t-BHP.
배양 간세포 (Chang)에서 황산화작용 및 항상화요소 활성에 미치는 계란 놀느자 단백질 가수분해물의 영향
박표잠,송병권,남경수,김세권 한국생명과학회 2000 생명과학회지 Vol.10 No.5
Normally, aerobic cells are protected from the damage of free radicals by antioxidative enzymes such as catalase, superoxide dismutase (SOD), glutathione (GSH) peroxidase and GSH-S-transferase. In this study, we have investigate the effect of egg yolk protein hydrolysates on antioxidative activity and the activity of antioxidative enzyme in cultured hepatocytes (Chang). Without the pretreatment with hydrolysate, about 50% of the hepatocytes were killed within 2h by 225$\mu$M tert-butyl hydroperoxide (t-BHP). By contrast, fewer than 20% of the 5 K hydrolysate (permeate from 5 kDa membrane and not passed through 1 kDa membrane)-pretreated hepatocytes were killed by the same concentrations of t-BHP. In addition, the activities of catalase, GSH peroxidase and GSH-transferase were significantly increasing with the treatment of 5 K hydrolysate. These results suggest that 5 K hydrolysate exerts antioxidative effect by increasing activity of antioxidative enzymes.
레시틴 추출 잔사인 계란노른자의 효소적 단백질 가순분해물의 항산화 특성
박표잠,정원교,최영일,김세권 한국생명과학회 2000 생명과학회지 Vol.10 No.2
Lecithin-free egg yolk protein (EYP), the by-product of lecithin extraction from egg yolk, which is denatured with an organic solvent, would normally be discarded. In this study, the denatured protein was renatured with alkali, and hydrolyzed with Alcalase in order to utilize by-product. The hydrolysate was separated through a series of ultrafiltration membranes with molecular weight cut-off (MWOO) of 10, 5 and 1 kDa, and the antioxidative activities of the hydrolysates was investigated. The 5K hydrolysate, permeate from 5 kDa membrane, showed stronger antioxidative activity than 10 K and 1 K hydrolysate which were permeated from 10 kDa and 1 kDa membrane, in a linoleic acid autoxidation system. In addition, the optimum concentration of antioxidative activity for 5 K hydrolysate was 1%, and the activity was about 37% higher as compared with α-tocopherol. The synergistic effect was also increased by using the hydrolysates with α-tocopherol.
박표잠,이상훈,김세권 한국막학회 2000 멤브레인 Vol.10 No.2
바지락 자숙액을 효과적으로 탈염하기 위하여 전기투석기를 사용하여 자숙액의 농도, pH 및 부피에 대한 최적 탈염조건을 검토하였다. 탈염에 필요한 이온교환막은 당과 단백질의 유실량이 적은 분자량 100 Da이상을 회수할 수 있는 AC-110-400을 선정하였으며, 자숙액 농도 0.5%와 4%의 탈염시간은 각각 100분과 170분에서 90%이상 염을 제거할 수 있었다. 자숙액의 pH에 대한 효과는 pH 9.0의 알칼리영역에서 보다 pH 4.0의 산성영역에서 탈염율이 더 높았으며, 투과액의 부피는 탈염시간과 탈염율에 거의 영향을 주지 않았다. 자숙액의 탈염은 주로 자숙액의 농도와 pH에 의해 크게 영향을 받았으며, 2% 자숙액 1 L, pH 5.75에서 효율적인 탈염이 가능하였다. For selective elimination of salt in the boiled extract of baby neck clam (Tapes variegata, BEBNC), electrodialyzer was used and the desalination conditions of BEBNC were determined at various concentrations, pH's and volumes of BEBNC. The ion-exchange membrane with a molecular weight cut off 100 Da was used for desalting of BEBNC. The desalination times on BEBNC concentrations of 0.5% and 4% were 100 min and 170 min and the final desalting ratio was above 90%. 1'he desalination of BEBNC at pH 4.0 was 12% higher than that at pH 9.0 for 60 min. The electrodialysis process could remove above 90% of the initial salt content in 2% BEBNC of 1 L for 80 min. The results obtained prove that electrodialysis is a practical solution to the problem of selective elimination of salt from BEBNC.
박표잠,Eun-Kyung Kim,이승재,Sun-Young Park,Dong-Soo Kang,정복미,김귀식,Jae-Young Je,안창범 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.1
Enzymatic hydrolysates of Laminaria japonica were evaluated for antioxidative activities using hydroxyl radical scavenging activity and protective effects against H2O2-induced DNA and cell damage. In addition, activities of antioxidative enzymes, including catalase, glutathione peroxidase, and glutathione S-transferase, of the enzymatic hydrolysates from L. japonica were also estimated. L. japonica was first enzymatically hydrolyzed by seven carbohydrases (Dextrozyme®, AMG®, Promozyme®, Maltogenase®, Termamyl®, Viscozyme®, and Celluclast® [all from Novo Co., Novozyme Nordisk, Bagsvaerd, Denmark]) and five proteinases (Flavourzyme®, Neutrase®, Protamex®, Alcalase® [all from Novo Co.], and pancreatic trypsin). The hydroxyl radical scavenging activities of Promozyme and pancreatic trypsin hydrolysates from L. japonica were the highest as compared to those of the other carbohydrases and proteinases, and their 50% inhibitory concentration values were 1.67 and 317.49 μg/mL, respectively. The pancreatic trypsin hydrolysates of L. japonica exerted a protective effect on H2O2-induced DNA damage. We also evaluated the protective effect on hydroxyl radical-induced oxidative damage in PC12 cells via propidium iodide staining using a flow cytometer. The AMG and pancreatic trypsin hydrolysates of L. japonica dose-dependently protected PC12 cells against cell death caused by hydroxyl radical-induced oxidative damage. Additionally, we analyzed the activity of antioxidative enzymes such as catalase, glutathione peroxidase, and the phase II biotransformation enzyme glutathione S-transferase in L. japonica-treated cells. The activity of all antioxidative enzymes was higher in L. japonica-treated cells compared with the nontreated cells. These results indicate that enzymatic hydrolysates of L. japonica possess antioxidative activity.
참치뼈로부터 Hydroxyapatite 소결체의 햄스터에 대한 구강점막자극시험
김세권,박표잠 한국생명과학회 2000 생명과학회지 Vol.10 No.6
In order to evaluate the safety of hydroxyapatite sinter produced from tuna bone in Syrian hamsters, oral mucous membrane irritation test was carried out. Oral mucous membrane irritation test was infected in Syrian hamsters as dose of 5 g/kg BW with hydroxyapatite sinder under pentobarbital sodium anesthesia. Each animal`s left cheek pouch was everted, and the samples were loosely placed at the bottom of the pouch with a double-suture technique for 14 days. Hamsters of control group were treated without inserting the hydroxylapatite sinter. Any abnormal clinical signs in both cheek pouches of control and treatment group were not observed for 14 days. There were no significant differences in body weight changes between hamsters of control and treatment group. Therefore, it suggest that hydroxyapatite sinter produced from tuna bone has no particular changes of oral mucous membrane irritation in Syrian hamsters.