Prostaglandin E1이 자궁근 통각수용체의 임펄스 발사에 미치는 영향

박정장,윤영욱,홍승길 고려대학교 의과대학 1988 고려대 의대 잡지 Vol.25 No.3

This study was performed to investigate the effect of prostaglandin El on the impulse discharge of uterine nociceptor activated by bradykinin, pain-producing substance. The extracellular recording of impulse discharge of uterine afferents was done at the distal stump of dorsal root S2-S4 in the cat anesthetized with pentobarbital sodium. Bradykinin and prostaglandin El were injected into the uterus through the micropolyethylene tube installed at the blanch of uterine artery. The results were summariaed as follows 1) Occurrence of an excitatory response to bradykinin in uterine nociceptor which could not be activated by this agent of same dose before injection of prostaglandin El. 2) After administration of prostaglandin El, increase in response magnitude to bradykinin in uterine nociceptor which already showed excitatory response to this agent of same dose. To summarize above results, it seems that prostaglandin E1 has the sensitizing effects on the uterine nociceptor activated by bradykinin.

복제산양(진순이)의 혈청학적 연구와 산자 생산

박희성,정수영,윤윤진,01성훈,박번영,문태식,정장용 한국동물번식학회 2006 Reproductive & Developmental Biology(Supplement) Vol.30 No.2s

복제산양(진순이)의 혈청학적 연구와 산자 생산

박희성,정수영,윤윤진,01성훈,박번영,문태식,정장용 한국동물번식학회 2006 Reproductive & developmental biology Vol.30 No.2

Rat H-Y 抗體가 생쥐 수정란의 발달단계에 따른 체외배양에 미치는 영향

鄭場龍,朴喜成,朴玉潤 진주산업대학교 농업기술연구소 1993 農業技術硏究所報 Vol.6 No.-

本 硏究는 免疫學的 방법에 의하여 性을 調節할 수 있는 방법을 개발하고자 近郊系統의 Wistar흰쥐의 수컷비장을 摘出하여 均質化시킨 다음 상충액을 취하여 同種의 암컷 腹膣內에 接種함으로서 H-Y抗原에 대한 H-Y 抗 血淸을 제조하였으며, 제조된 H-Y抗 血淸과 함유한 培養液에 ICR系統의 생쥐로부터 각 발달단계별로 채란한 수정란을 NaHCO3-BMOC-3 배양액에 H-Y항 혈청과 보체를 첨가하여 CO_2 incubator에서 24∼30시간 배양을 실시하였다. 본 연구에서 얻은 결과는 다음과 같다. H-Y 抗體의 形成은 rat 10마리중 8마리에서 抗體가 형성되어 80%의 形成率을 보였으며, 427개의 수정란을 H-Y 抗體를 處理하여 이중 182개(43%)가 파괴 되었다. H-Y 抗血淸 및 補體의 存在下에서 培養된 2-, 4-, 8-, 16-세포기, 상실배 및 胞胚期 수정란의 파괴율은 각각 0.0, 42.9, 54.1, 47.0, 48.1 및 47.5%로서 2-세포기와 4-세포기 以上間에는 유의적 (P<0.05)인 差異가 있었으나, 4-세포기 이상과 胞胚期 受精卵間에는 H-Y 항체가 미치는 영향은 유의적(P<0.05)인 차이가 없었다. This experiment was carried out to develop a new technique of identifying XX or XY-bearing mouse embryos by immunological method. H-Y antiserum prepared in inbred Wistar female rats by repeated immunization with spleen cells from males of the same strain. The reactivity of H-Y antibody was confirmed by culturing mouse embryos in the medium containing H-Y antiserum and complement obtained from the guinea pig. The optimal condition for the activity of H-Y antibody was also investigated by culturing embryos under the concentration or affected H-Y antibody and in vitro culture rate. The bilological test with the 8- or 16-cell stge embryos showed H-Y antibody was formed in all female rats immunized with spleen cell, but it was formed only in 80% female rats immunized with the antigen. When the embryos were in vitro cultured in the medium of H-Y antiserum and complement the lysis rate of 2-, 4-, 8-, 16-, morula and blastocyst was 0.0, 42.9, 54.1, 47.0, and 47.5% respectively, but significantly(P<0.05) low than that of 2-cell stage.

산욕기 자궁내번증 1예

박정장(JJ Park),하기성(GS Ha),주재본(JB Choo) 대한산부인과학회 1981 Obstetrics & Gynecology Science Vol.24 No.4

A case of pureperal inversion of the uterus is presented with successful reposition and briefly reviewed.

Krukenberg 종양의 1예

박정장(JJ Park),최숙(S Choi),하기성(GS Ha),김상철(SC Kim) 대한산부인과학회 1979 Obstetrics & Gynecology Science Vol.22 No.7

위장에서 발생한 것으로 사료되는 비교적 희유한 양측성인 전이성 종양 1예를 경험하였기에 보고하고 간단한 문헌적 고찰을 하였다. There have been a case of Krukenbergs tumor in ovary being the primary site in the stomach. The patient were 40 years old woman living now afer the surgery, and here we represent case presention and review of literatures was made being briefly.

재래산양에 있어서 핵이식란의 융합조건이 융합 및 체외발달에 미치는 영향

박희성,김태숙,이윤희,정수영,이명열,홍승표,박준규,김충희,정장용 한국동물생명공학회(구 한국동물번식학회) 2004 Reproductive & Developmental Biology(Supplement) Vol.28 No.2s

본 연구는 재래산양의 핵이식을 실시하여 공여세포의 조건, 전기적 세기 및 융합횟수 등이 융합율과 체외발달율에 미치는 영향을 조사하여 최적의 융합조건을 확립하고자 실시하였다. 공여세포는 귀 유래 섬유아세포와 태아 유래 섬유아세포 2종류를 분리 배양하여 사용하였으며, 수핵란의 채취는 성숙한 미경산 재래산양에 과배란을 유기하여 hCG 투여 후 제 35시간째에 외과적인 방법으로 in vivo (체내성숙)난자는 난관을 관류하는 방법으로 회수하고 in vitro (체외성숙)난자는 난포로부터 흡입하여 난포란을 채취하여 약 22시간 체외성숙을 실시하였다. 수핵난자는 난구세포를 제거한 다음 0.05 M sucrose를 처리하여 세포질이 양호하고 극체가 뚜렷하게 보이는 난자만을 선별하여 핵이식을 실시하였다. 핵이식란의 융합은 전기자극방법으로 융합을 실시하였으며, 핵이식 조작 후 약 3시간 동안 전배양을 실시한 다음 활성화를 유도하였다. 복제수정란은 0.8% BSA가 첨가된 mSOF 배양액으로 6∼7일 동안 체외 배양을 실시하였다. 귀 유래 섬유아세포를 공여세포로 사용하였을 때 융합율은 60.4%로서 태아 유래 섬유아세포의 40.3%보다는 높게 나타났다. 분할율에 있어서는 귀 유래 섬유아세포와 태아 유래 섬유아세포가 각각 47.6 및 48.2%로서 차이가 없었다. 2.40∼2.46 ㎸/㎝로 전기자극을 주었을 때 융합율은 43.8%로서 1.30∼l.40 ㎸/㎝(26.7%)와 2.30∼2.39 ㎸/㎝ (34.8%)가 높게 나타났으며, 융합이 이루어진 핵이식란의 분할율은 82.9(1.30∼l.40 ㎸/㎝), 43.8(2.30∼2.39 ㎸/㎝) 및 51.8%(2.40∼2.46 ㎸/㎝)로서 전기자극의 세기에 따른 유의적(p<0.05)인 차이는 없었다. 전기융합을 1회 실시하였을 때 in vivo 난자는 43.5%로서 in vitro 난자의 23.6%보다 유의적으로 높게 나타났으며, 2회 실시하였을 때는 55.7(in vivo) 및 39.2%(in vitro)로 in vivo에서 높게 나타났다. 3회 자극을 주어 전체 융합율은 in vivo가 66.1%로서 in vitro의 52.8%보다는 유의적으로 높게 나타났다. This study was conducted to examine the effects of electric stimulation conditions on in vitro developmental ability of caprine embryos after somatic cell nuclear transfer. Recipient oocytes were surgically collected after superovulation by using CIDR and FSH, PMSG, hCG and estrous synchronization in Korean native goats. The caprine ear cells were cultured in vitro in serum-starvation condition (TCM-l99 + 0.5% FBS) for 3 to 5 days of cell confluence. The zona pellucida of in vivo and in vitro matured oocytes were partially drilled using laser system. Single somatic cell was individually transferred into the enucleated oocyte. The reconstructed oocytes were electrically fused with 0.3M mannitol. After the electofusion, embryos were activated by electric stimulation or Ionomycin + 6-DMAP. Nuclear transfer embryos were cultured in mSOF medium supplemented with 0.8% BSA 6∼7 days at 39 , 5% CO₂, 5% O₂, 90% N₂. The fusion rate of donor cells was 60.4% and 40.3 % in ear cell and fetal fibroblast, and cleavage rate were 40.6% and 48.2%, respectively. No significant difference was found in the fusion and cleavage rate in different donor cells. Nuclear transferred oocytes were fused by electric pulses of 1.30∼1.40, 2.30∼2.39 and 2.40∼2.46 ㎸/㎝. There was no significant difference among different electric pulses in fusion rates (26.7, 34.8 and 43.8%). The cleavage rate was higher (p<0.05) in 1.30∼1.40 ㎸/㎝ (82.9%) than 2.30∼2.39 ㎸/㎝ (43.8%) and 2.40∼2.46 ㎸/㎝. (51.8%). The fusion rates of recipient oocyte source were 1st (43.5% and 23.6%), 2nd (55.7% and 39.2%) and 3rd (66.1% and 52.8%) in in vivo and in vitro oocytes. However, fusion ratee were significantly higher (p<0.05) in in vivo than in vitro oocyte. The cleavage rate of fused oocytes from in vivo and in vitro sources were 52.6% and 54.4%, respectively. No significant difference was found in the cleavage rate according to the recipient oocyte source. These results suggest that factors such as field pulse of electric stimulation and oocyte source could affect in vitro developmental ability of nuclear transplanted caprine oocytes.

재래산양의 체세포 핵이식에 의한 복제수정란의 체외발달에 관한 연구

박희성,김태숙,정수영,이윤희,정장용 韓國受精卵移植學會 2005 한국동물생명공학회지 Vol.20 No.2

본 연구는 재래산양에서 복제 수정란의 생산효율을 향상시키기 위한 기초 자료를 제시하고자 체세포 핵이식을 실시하여 공핵세포의 종류, 핵이식란의 활성화 처리 방법 및 수핵난자의 조건이 체외발달율에 미치는 영향을 조사, 검토하여 핵이식란 생산을 위한 최적의 조건을 규명하고자 실시하였다. 공핵세포의 종류에 따른 핵이식란의 체외발달율은 융합이 이루어진 핵이식란의 활성화 처리 후 분할율은 귀 유래 섬유아세포를 공핵세포로 사용하였을 때가 로서 태아 유래 섬유아세포를 The present study was conducted to examine some factors affecting in vitro development of oocytes from somatic cell nuclear transfer (SCNT) in Korean native goats. Recipient oocytes were surgically collected after superovulation by using CIDR and FSH, PMSG, hCG and estrous synchronization in Korean Native goats. For nuclear transfer, the fibroblasts from caprine ear cells and fetal fibroblasts were surgically harvested and were cultured in vitro until cell confluency in serum-starvation condition (TCM-199 + FBS) for 3 to 5 days. The zona pellucidae of matured oocytes were partially drilled by laser irradiation. A single somatic cell was individually transferred into each enucleated oocyte. The reconstructed oocytes were then electrically fused and activated. Activated NT embryos were cultured in mSOF medium supplemented with in air. There were no significant difference in the number of embryos cleaved and 4-cell development between the fibroblast nuclei from mature ear cells and fetal cells, but the rate of 8-cell development was higher (P<0.05) in ear cells than in fetal cells . However, the embryo development to morula or blastocyst was not significantly different between both the groups, respectively. The number of embryo cleaved were higher (P<0.05) in the oocytes activated with ionomycin+6-DMAP than in the oocytes activated electrically . The development of fused embryos to morula or blastocyst was found in ionomycin+6-DMAP, but no morula or blastocysts were developed in electrical stimulation. The development rate of SCNT embryos to morula or blastocyst was love. (P<0.05) in SCNT embryos than that in parthenotes . In the parthenotes, the cleavage rate and development to morula or blastocyst were significantly higher (P<0.05) as in ovulated oocytes than in follicular oocytes , respectively. These results suggest that some factors Including superovulation treatment, oocyte source, maturation of follicular oocytes, activation method and culture condition may affect in vitro developmental capability of embryos produced by somatic cell nuclear transfer in Korean Native goats, and the fusion rate be greatly low compared with other species.

발단 단계별로 분할한 생쥐 수정란에 Rat H-Y 항체 처리가 분할란의 발달에 미치는 영향

정장용,박희성,문승식,박성진 진주산업대학교 1993 論文集 Vol.32 No.-

This experiment was carried out to develop a new technique of identifying XX or XY-bearing bisected embryos prior to implantation by immunological method. H-Y antiserum prepared in inbred Wistar female rats by repeated immunization with spleen cells from males of the same strain. The reactivity of H-Y antibody was confirmed by culturing mouse embryos in the medium containing H-Y antiserum and complement obtained from the guinea pig. The optimal condition for the activity of H-Y antibody was also investigated by culturing embryos under the concentration or affected H-Y antibody and culture rate. The bisection rate of mouse embryo at 4-cell stage(64.6%) was significantly(P<0.05) low than 8-cell(76.0%), morula(81.2%) or blastocyst(81.3%). And not significantly(P<0.05) different from 4 to 8-cell and morula to blastocyst stage embryos. When the bisected embryos without any damage from microsurgery were cultured in vitro up to twin blastocyst or twin reformated blastocyst, the in vitro development of demi-embryos bisected at morula to blastocyst was 81.6 to 84.7%. However, the in vitro development of demi-embryos bisected at 4-cell(31.6%) to 8-cell(37.8%) stage was significantly(P<0.05) lower. The lysis-rate of bisected 4- to 8-cell and morula to blastocyst embryo was 42.7 and 58.6%. The morphology embryos of zona-free and intact embryos showed the embryos lysis rate of 48.5 and 42.7% respectively(P<0.05).

재래산양에 있어서 핵이식란의 융합조건이 융합 및 체외발달에 미치는 영향

박희성,김태숙,이윤희,정수영,이명열,홍승표,박준규,김충희,정장용 사단법인 한국동물생명공학회 2004 Reproductive & developmental biology Vol.28 No.2