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Adriamycin이 白鼠 培養心筋細胞와 內皮細胞에 미치는 細胞毒性에 관한 硏究
朴承澤,金錤元,鄭然泰 圓光大學校 醫科學硏究所 1988 圓光醫科學 Vol.4 No.1-2
In an attempt to evaluate the cytotoxicity of adriamycin, an anticancer drug, colorimetric assays of neutral red(NR), tetrazolium MTT, lactate dehydrogenase activity and protein content were investigated on myocardial and endothelial cells from the newborn rat heart grown for 96 hours in media containing adriamycin of various concentrations. Light and electron microscopic studies were also carried out on these cells. Initial and midpoint cytotoxicities of adriamycin in myocardial cells were detected at lower concentrations in the NR assay(NR-90, 0.07 ㎍/ml ; NR-50, 2.0㎍/ml) than in the MTT assay (MTT-90, 0.15㎍/ml ; MTT-50, 2.8㎍/ml). However, in endothelial cells, they were noted to be at lower concentrations in the MTT assay(MTT-90, 0.1㎍/ml ; MTT-50, 2.3㎍/ml) than in the NR assay (NR-90, 0.3㎍/ml ; NR-50, 4.1㎍/ml). Protein content in myocardial cells treated with adriamycin, NR-50 and MTT-50 were calculated to be 38.1 % and 34.1 % to the control respectively and these in endothelial cells grown in media containing adriamycin, NR-50 and MTT-50 were 36.4% and 45.7 % to the control respectively. With addition of adriamycin, increase in amount of LDH was noted in media of both myocardial and endothelial cells and was dose-dependent of adriamycin. In light microscopy, both myocardial and endothelial cells were observed to decrease in number and shape of these cells became more spherical than that of the control with adriamycin treatment. Electron microscopy of adriamycin treated cells showed increment of lysosome and vacuoles, swelling of mitochondria and cisternal dilatation of rough endoplasmic reticulum. These results suggest that adriamycin inhibits in vitro proliferation and growth of the cells by disturbing the cell metabolism.
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박승택,윤향석,형근영,조정구,이강창,김원신,김형민,전병훈,윤용갑,Park Seung-Taeck,Yoon Hyang-Suk,Hyoung Keon-Young,Cho Chung-Gu,Lee Kang-Chang,Kim Won-Shin,Kim Hyung-Min,Jeon Byung-Hoon,Yun Young-Gap 대한한의학방제학회 1999 大韓韓醫學方劑學會誌 Vol.7 No.1
In order to eludidate the mechanism of oxidative stress in cultured spinal motor neurons damaged by oxygen free radicals, cytoxicity was assesed by MTT assay and NR assay after spinal motor neurons from mouse were cultured in media containing various concentrations of xanthine oxidase(XO) and hypoxanthine(HX) for 3 hours. In addition, neuroprotective effects of several herb extracts on oxidant-induced neurotoxicity were examined in these cultures, compared with nerve growth factors such as basic fibroblast growth factor(bFGF). XO/HX decreased cell viability in dose- and time dependent manners on cultured mouse spinal motor neurons, and MTT50 and NR50 values were measured at 20mU/ml XO and 0.1mM HX for 3 hours in these cultures. bFGF significantlt increased cell viability. In neuroprotective of herb extracts, Epimedium Koreanum Nakai(EK) and Alpinia oxphylla Mig(IJI) was very effective in the prevention of the neurotoxicity induced by XO/HX in cultured mouse spinal motor neurons. From the above results, it is suggested that XO/HX shows toxic effect in cultured mouse spinal motor neurons and selective herb extracts such as Epimedium Koreanum Nakai(EK) and Alpinia oxphylla Mig(IJI) were very effective in the increase of cell viability against the neurotoxicity induced by oxygen radicals in these cultures.
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6價크롬의 생쥐의 初期發生段階에 미치는 影響에 關한 形態學的 硏究
朴承澤,金正雄,禹元洪,崔玟圭,鄭然泰 圓光大學校 醫科學硏究所 1987 圓光醫科學 Vol.3 No.1
Mouse two-cell embryos were cultured in the medium containing various concentrations of Cr^(6+) for 72 hours in order to elucidate the cytotoxicity of heavy metal ion and also biochemical observation was performed to measure the protein synthesis in early embryos which were injected with ^3H-leucine for two hours. The results were summerized as follows. 1 - Chromium ion(Cr^(6+)) inhibited remakably the development of mouse early embryos at 1 ppn, when two - cell embryos were cultured for 72 hours at various concentrations of chromium ion. 2. Chromium ion(Cr^(6+)) retarded or suppressed the cleavage of early embryos. Fusion, fragmentation, cytolysis and developmental retardation of blastomeres were observed in the each developmental stage of embryonal cells. 3. Chromium ion(Cr^(6+)) suppressed markedly the incorporation of ^3H-leucine, but didn't give an influence on the uptake of precursor by examing the two-cell embryos exposed to chromium ion for 6 and 24 hours respectively. From this result, it suggest that chromium ion(Cr^(6+)) might suppress the development of early embryos by inhibiting the protein synthesis.
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Glucose Oxidase에 의(依)하여 손상(損傷)된 배양척수감각신경절세포(培養脊髓感覺神經節細胞)에 대(對)한 음양곽(淫羊藿)의 효과(效果)
박승택,이호섭,윤용갑,박병림,Park Seung-Taeck,Lee Ho-Sub,Yun Yong-Gap,Park Byung-Rim 대한한의학방제학회 1999 大韓韓醫學方劑學會誌 Vol.7 No.1
척수감각신경절세포에 대한 산소자유기의 신경독성효과에 대한 기전을 규명하기 위하여 여러 농도의 Glucose Oxidase(GO)를 배양 척수 감각신경절세포에 처리한 후 GO의 독성효과를 분석하였으며 또한 GO에 의하여 유발된 신경독성에 대한 음양곽(Epimedium Koreanum Nakai)의 방어효과를 MTT assay법에 의하여 조사하여 다음과 같은 결론을 얻었다. GO는 신경세포에 처리한 농도와 시간에 비례하여 세포의 생존율을 유의하게 감소시켰으며, 또한 음양곽이 GO의 독성효과를 효과적으로 방어하였다. 이상의 결과로부터 산소자유기인 GO는 생쥐의 배양 척수감각신경절세포에 독성을 나타냈으며 음양곽과 같은 한약추출물이 GO의 독성을 방어하는데 효과적인 것으로 나타났다. To evaluate the neurotoxic effect of oxygen radicals in cultured mouse spinal dorsal root ganglion(DRG) neurons, cytotoxicity was determined by MTT assay after cultured DRG neurons were grown in the media containing various concentrations of glucose oxidase(GO). In addition, neuroprotective effect of herb extract, Epimedium Koreanum Nakai was examined by MTT assay in cultured DRG neurons. Cell viability of cultured DRG neurons was remarkably decreased by GO in dose- and time-dependent manner, and Epimedium Koreanum Nakai protected remarkably GO-induced neurotoxicity in these cultures. From the above results, it is suggested that oxygen radicals is toxic in cultured mouse DRG neurons, and herb extracts such as Epimedium Koreanum Nakai are effective in prevention of the neurotoxicity induced by oxygen radicals in cultured mouse DRG neurons.
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고혈당 환경에서 배양한 생쥐 척수후근 신경절 세포의 변화 및 신경성장인자의 영향
박승택,김기훈,장근영,조정구,박병현 대한당뇨병학회 2001 Diabetes and Metabolism Journal Vol.25 No.4
Background: Multiple etiology of diabetic neuropathy has been proposed, including altered polyol metabolism, superoxide radical formation, protein glycation, vascular insufficiency, blunted nitric oxide production and neurotrophic factor (NTF) deficiency. Nerve growth factor (NGF) is a member and family of neurotrophic factors. NGF is produced in tissues innervated by its responsive neurons. In the peripheral nervous system, NGF messenger RNA (mRNA) is produced in target fields of small pain and temperature-mediating dorsal root ganglia (DRG) sensory neurons and sympathetic neurons. NGF has been shown to promote their survival, differentiation, and maintenance. However, the mechanism of neuronal damage in diabetes and the effect of NGF on diabetic neuropathy are not clear. Methods: In order to clarify the effect of NGF, the changes of cell viability were evaluated by MTT assay on mouse cultured dorsal root ganglion cells which were grown with media containing concentrations of high glucose for inducing hyperglycemic condition. Furthermore, the neuroprotective effect of nerve growth factor (NGF) against hyperglycemia-induced dorsal root ganglion cell changes were also examined. Results: 1. Cell viability of cultured mouse dorsal root ganglion cells treated with hyperglycemic media made with 15, 25 mM glucose was markedly decreased in a dose-dependent manner when compared with control medium (normoglycemic medium) containing concentration of 5.5 mM glucose (p$lt;0.05). 2. Cultured dorsal root ganglion cells exposed to hyperglycemic medium made with 25 mM glucose for 72 hours showed morphological changes such as dissociations, loss of neurites and decrease of cell viability (p$lt;0.05). 3. Pretreatment of 150 ng/mL NGF for 2 hours significantly increased the cell viability of cultured dorsal root ganglion cells which exposed to hyperglycemic medium (25 mM glucose for 72 hours). Conclusion: Findings from this study suggested that hyperglycemic condition induces the decrease of cell viability and morphological changes (loss of neurites, dissociation) on cultured dorsal root ganglion cells of mouse. Furthermore, selective neurotrophic factors such as NGF are very effective in preventing dysfunction and morphological changes of DRG cells induced by hyperglycemic condition.
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박승택,김정우,정석희,서영미 기초간호학회 2012 Journal of korean biological nursing science Vol.14 No.2
창상의 회복효과를 높이기 위해 보다 나은 치료물질 또는 치료방법을 찾고자 연구자들의 계속된 노력이 이루어지고 있다(Kwon, Choi, Yoon, & Ki, 2010; Nam, 2010; Zhao, 2009). 이렇게 관심을 가지는 치료물질 중에 허브가 있으며, 이것들을 치료제로 개발하고자 효능 분석과 효과에 대한 연구가 진행되고 있다(Battaglia, 2003; Kwon et al., 2010; Nam, 2010). 최근 간호학에서도 허브에서 추출한 에센셜 오일(essential oil)을 창상치유 간호에 적용하는 것에 관심을 보이고 있다(Hur & Han, 2004). 이러한 허브는 서양에서 오래전부터 각종 질환의 치료나 예방에 사용되었는데 이는 식물의 함유성분과 일부분을 차지하고 있는 세포내에서 생산되는 소량의 정유 성분에 의한 것으로 알려져 있다(Rodr, Zullyt, Merino, & Schulz, 2007). 특히, 정유는 일명 에센셜오일로도 불리우며, 일종의 방향족 화합물로서 다른 세포함유성분과 함께 유효한 생리활성을 나타낸다고 알려져 있다(Owlia, Rasooli, & Saderi, 2007). 허브의 함유성분을 구성하고 있는 화학물질들의 합성경로는 매우 다양하지만, 그 중에서도 메발론산경로(mevalonic pathway)를 통해 만들어지는 터핀류(terpenes)와 시키믹경로(shikimic pathway)에 의한 페닐프로판류(phenylpropane)가 가장 많은 비중을 차지하고 있다(Gould, 1997). 이들에 의하여 생산된 물질성분들은 여러 질환에 유효한 효능을 나타낸다고 알려져 있다. 예를 들면, 터핀류중 세스퀴터핀에 속하는 카마쥴렌(chamazulene)은 종양억제나 흉터에 효과가 있으며, 캐모마일(chemomile)에 다량 함유되어 있다(Safayhi, Sabieraj, Sailer, & Ammon, 1994). 또한 케톤류(ketons)는 흉터나 피부재생에 유효하며, 라벤더(laven기초간호자연과학회지der)나 제라늄(geranium) 등에 다량 포함되어 있으며(Lee, 2007), 터핀알콜류는 향이 상쾌하며 살균이나 피부재생, 항균에 효과가 있으며, 티트리(tea tree)나 스위트마조람(sweet majoram) 등에 다량 함유되어 있다(Moleyar & Narasimham, 1992). 이와 같이 저먼캐모마일(Matricaria chamomila)은 동상은 물론 여드름이나 습진과 같은 피부질환, 항염, 항균 등에 효과적이어서 인도와 유럽을 중심으로 한 동서양에서 오래전부터 가정상비약의 하나로 사용되어 왔다(Lee, Min, Sin, & Kim, 2008). 또한, 라벤더(Lavendula angustifolia)는 향
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