Ultrasound-Guided Lateral Femoral Cutaneous Nerve Conduction Study

박범준,양승남,정의수,최준규,강석,윤준식 대한재활의학회 2015 Annals of Rehabilitation Medicine Vol.39 No.1

Objective To verify the utility of the lateral femoral cutaneous nerve (LFCN) ultrasound-guided conduction technique compared to that of the conventional nerve conduction technique.Methods Fifty-eight legs of 29 healthy participants (18 males and 11 females; mean age, 42.7±14.9 years) were recruited. The conventional technique was performed bilaterally. The LFCN was localized by ultrasound. Cross-sectional area (CSA) of the LFCN and the distance between the anterior superior iliac spine (ASIS) and the LFCN was measured. The nerve conduction study was repeated with the corrected cathode location. Sensory nerve action potential (SNAP) amplitudes of the LFCN were recorded and compared between the ultrasound-guided and conventional techniques. Results Mean body mass index of the participants was 23.7±3.5 kg/m2, CSA was 4.2±1.9 mm2, and the distance between the ASIS and LFCN was 5.6±1.7 mm. The mean amplitude values were 6.07±0.52 μV and 6.66±0.54 μV using the conventional and ultrasound-guided techniques, respectively. The SNAP amplitude of the LFCN using the ultrasound-guided technique was significantly larger than that recorded using the conventional technique. Conclusion Correcting the stimulation position using the ultrasound-guided technique helped obtain increased SNAP amplitude.

소통으로서의 문화콘텐츠학 담론 모색과 ‘배치’를 통한 콘텐츠 기획 연구: 영화 <Life in a Day> 사례분석을 중심으로

박범준 글로벌 문화콘텐츠학회 2012 글로벌문화콘텐츠 Vol.0 No.8

Today, The interest in culture is continuously rising. It has a relation popular department with culture. Department of Cultural Contents are enrolled in this study, the culture. ‘Culture’ has been proceeding in the center of various debate in other cultures ahead of us. This is shown thinking of Culture each other. Study of Cultural contents must be contained for the recognition about our culture when we discuss about cultural practice and understand. The study of Cultural contents direction come out, if we arrangement the culture concept according to the historical background. I will say that communication is culture. Culture is the place to take empathy with rationality, emotion, intellect, spirituality. Communication is empathy for the coexistence. And the structure is done by Sotong(communication)-Hami(person), contents, communication semantic, media. Communication is the ‘media-meaning-network’ construction. However,Sotong-Hami in order to create meaning and relation is only possible through empathy. To meet current and demands the flow of culture with empathy, Study of cultural contents research methods will be able to improve vision. 오늘날 ‘문화’는 우리 삶의 중요한 화두이다. 문화콘텐츠도 이름에서 알 수있듯이 문화와 뗄 수 없는 관련을 맺고 있다. 최근에 이루어진 문화에 대한논의들을 시대적인 흐름에 따라 간략하게 몇 가지 특성들로 정리를 한다면, 이를 토대로 과연 문화콘텐츠학에서는 어떠한 관점에서 문화를 이해하고 어떻게접근할 수 있을지에 대한 물음을 던질 수 있을 것이다. 나는 문화가 이성ㆍ감성ㆍ지성ㆍ영성적 차원에서 소통과 공감이 이루어지는 현장이라고 보고 문화를 소통으로 규정하고자 한다. 소통은 공존을 위해서 콘텐츠를 이용한 교감이며 공감이다. 이것의 구성 요소는 소통하미, 소통거리, 소통걸이, 소통다리이다. 소통의 다리 역할을 하는미디어는 각각의 형식적 특성을 갖는다. 특히 디지털(점형표현) 미디어에서는다양한 정보표현요소인 소리, 텍스트, 이미지(정지영상, 동영상)를 이리저리 주물러 하나의 의미체제(콘텐츠)로 조직해 낸다. 이렇게 만들어진 콘텐츠는 사람ㆍ시간ㆍ공간의 차원을 표현하고 연결해 준다. 소통거리(콘텐츠)를 통한 소통은 미디어(media)-의미(meaning)-관계(network)의 구조 속에서 일어난다. 그러나 이것은 의미와 관계를 만들어내기위해서 소통하미가 시도하는 세상과의 공감이 있기에 가능한 것이다. 소통하미가 먼저 관심이 있는 존재에 대해 공감하고, 이것을 콘텐츠화하여 들으미(수용자)도 공감할 수 있도록 콘텐츠화하는 것이다. ‘문화’의 의미를 소통의 요소이자 계기로 삼아 그 형식과 특성 그리고 구조를 분석하는 것을 통해 문화콘텐츠학의 역할과 가능성을 점검해 본다.

정상인에서 신체적 요인이 체중 치우침과 입각기에 미치는 영향

박범준,박시복,박종우,이승조,이규훈 대한재활의학회 2008 Annals of Rehabilitation Medicine Vol.32 No.5

Objective: To determine if physical factors (age, sex, weight, foot length) affected anteroposterior and intersidal weight- bearing pattern on footplate in standing posture and duration of stance phase at walking.Method: Participants were 578 healthy adults (250 men, 328 women). All participants were tested by GaitviewⓇ (Alfoots, Seoul, Korea) which measure anteroposterior, intersidal weight-bearing pattern on foot plate and duration of stance phase at walking. Measurements were analyzed to find any relations among physical factors (age, sex, weight, foot length), anteorposterior, intersidal weight-bearing pattern and stance phase. Results: The age, weight, foot length had no specific relations to anteroposterior weight-bearing pattern (r<0.2, p<0.05). Men had greater weight-bearing pattern on forefoot than women (p<0.01). The higher the age, the more midstance and the more delayed stance phase was noted (r>0.4, p<0.01).Conclusion: There was a significant correlation between sex and anteroposteior weight-bearing pattern. And there was a positive relation between age and stance, midstance phase.

RAPD-PCR(Random Amplified Polymorphic DNA - Polymerase Chain Reaction) 방법을 이용한 Listeria monocytogenes의 검색

박범준,신언환 한국식품영양학회 2004 한국식품영양학회지 Vol.17 No.3

Rapid detection of foodborne pathogens is becoming increasingly important. The requirement for faster, more reliable tests has lead to the development of a wide range of rapid methods. Among these methods, the use of systems based on nucleic acid based detection has been increasing since they offer advantages of reduction in test time and more reliable detection or identification. Random Amplification Polymorphic DNA(RAPD) method has been used to fingerprint foodborne microorganisms; Listeria monocytogenes. In this study, 10-mer primer OPG-13(5'-CTCTCCGCCA-3') was used to generate RAPD-PCR for detection of pathogenic L. monocytogenes of Listeria spp. Among 20 primers tested, OPG-13 showed on acceptable result for the differentiation of a pathogenic Listeria from non-pathogenic microorganisms. Pathogenic Listeria, L. monocytogenes(ATCC 15313, 19111, 19112, 19113) showed two bands for 700 bp and 1,500 bp while non-pathogenic bacteria, L. ivanovii, L. grayi, L. murrayi, L. innocua, L. welshimeri, and L. seeligeri had only one band sizing from 2,000 to 2,300 bp. This RAPD method proved to be a valuable to gain important information on sources of pathogenic bacteria in food industry.

고분자 전해질 연료전지용 수소극 촉매층의 이오노머 함량 영향

박범준,이선호,우승희,박석희,정남기,임성대 한국수소및신에너지학회 2019 한국수소 및 신에너지학회논문집 Vol.30 No.6

For the low-Pt electrodes for polymer electrolyte fuel cells (PEMFCs), the optimization of ionomer content for anode catalyst layers was carried out. A commercial catalyst of 20 wt.% Pt/C was used instead of 50 wt.% Pt/C which is commonly used for PEMFCs. The ionomer content varies from 0.6 to 1.2 based on ionomer to carbon ratio (I/C) and the catalyst layer is formed over the electrolyte by the ultrasonic spray process. Evaluation of the prepared MEA in the unit cell showed that the optimal ionomer content of the air electrode was 0.8 on the I/C basis, while the hydrogen electrode was optimal at the relatively high ionomer content of 1.0. In addition, a large difference in cell performance was observed when the ionomer content of the hydrogen electrode was changed. Increasing the ionomer content from 0.6 to 1.0 by I/C in a hydrogen electrode with 0.05 mg/cm2 platinum loading resulted in more than double cell performance improvements on a 0.6 V. Through the analysis of various electrochemical properties in the single cell, it was assumed that the change in ionomer content of the hydrogen electrode affects the water flow between the hydrogen and air electrodes bounded by the membrane in the cell, which affects the overall performance of the cell. A more specific study will be carried out to understand the water flow mechanism in the future, and this study will show that the optimization process of hydrogen electrode can also be a very important cell design variable for the low-Pt and high-performance MEA.

노화 흰쥐 소뇌에서 세포사 조절인자들의 변화에 대한 연구

박범준,정윤희,김성수,김경용,이원복 대한체질인류학회 2007 해부·생물인류학 (Anat Biol Anthropol) Vol.20 No.2

In the present study, we investigated the expression of apoptosis-associated proteins in the cerebellum of aged rats: IGF-I receptor (IGF-IR), nitrotyrosine (NT), p53, key pro-apoptotic gene ICH-1 (caspase-2), c-Fos and Bcl-2 family members (Bcl-2 and Bax). Twelve adult (4~6 month old) and 15 aged (24~29 month old) Sprague-Dawley rats were examined in this study. We performed immunohistochemical staining, in situ hybridization and densitometric measurement using a NIH image program (Scion Image) to determine the staining density. In adult rats, there were no immunoreactivities for insulin-like growth factor-I receptor (IGF-IR), nitrotyrosine (NT) or p53 in any region of cerebellum. However, IGF-IR immunoreactivity was found in some Purkinje cells in aged rat cerebellum. The prominent staining of NT or p53 was also localized in the Purkinje cell layer in aged rats. A high density of ICH-1 (caspase-2) immunoreactivity was observed in the molecular and Purkinje cell layers in aged rats. Immunoreactivity for c-Fos was significantly decreased in the granule cells in aged rats. Positive signal for bcl-2 was significantly decreased in the Purkinje cells and granule cells of aged rats. The most intense staining for Bax was observed in the soma of Purkinje cells of adult rats. However, Bax immunoreactivity was not changed in any layers in the cerebellar cortex of aged rats. In conclusion, this study provides the first morphological data concerning the differential regulation of apoptosisrelated genes in rat cerebellum during aging. 세포자멸사는 발생과정과 중추신경계의 항상성 유지에서 중요한 역할을 하는 것으로 알려져 있다. 본 연구에서는 흰쥐 소뇌에서 노화에 따른 세포사 조절인자들의 변화를 in situ hybridization법과 면역조직화학 법을 사용하여 관찰하였다. TUNEL 염색 결과에서는 염색된 세포핵이 노화 흰쥐 소뇌에서만 발견되었다. IGF-IR, NT 및 p53에 대한 면역 염색성은 성숙군의 소뇌에서는 거의 관찰되지 않은 반면, 노화군에서는 소뇌 겉질의 조롱박 세포에서 현저한 면 역염색성을 보였다. ICH-1 (caspase-2)의 면역염색성은 노화 쥐의 분자층과 조롱박세포층에서 관찰되었다. c-Fos는 성숙군의 과립층에서 진하게 염색되었고, 면역염색성은 노화군의 과립층에서 유의성 있게 감소하였다. Bcl-2는 조 롱박 세포 (Purkinje cell)층과 과립층에서 가장 강하게 발현되었고 노화에 따라 유의성 있게 감소하는 경향을 나 타냈다. Bax는 성숙군의 조롱박 세포에서 가장 진하게 염색되었고, Bax 면역염색성은 소뇌 겉질과 소뇌핵에서 노 화에 따른 유의성 있는 차이를 나타내지 않았다. 결론적으로, 본 연구는 흰쥐 소뇌에서 세포사 관련인자들의 노화에 따른 변화에 대한 최초의 형태학적 자료를 제공하였다. 이러한 연구는 노화를 비롯한 여러 신경퇴행성 질환 연구에 필요한 기본적인 형태학적 자료를 제공 할 수 있을 것이라 사료된다.

Visual colorimetric detection of ammonia under gaseous and aqueous state: Approach on cesium lead bromide perovskite-loaded porous electrospun nanofibers

박범준,김수헌,곽철환,Kugalur Ranjith Shanmugam,한영규,조영진,허윤석 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.97 No.-

In this work, we fabricate porous cesium lead bromide nanofibers (CsPbBr3 NFs) via electrospinning andemploy them in a sensor to effectively detect both gaseous and aqueous ammonia. The CsPbBr3 NFs areproduced byfirst mixing cesium lead bromide nanocrystals (CsPbBr3 NCs) with polystyrene (PS),followed by solidifying the mixture into NFs using electrospinning. Porous NFs are modified using thevolatile solvent toluene, which is volatilized during the electrospinning process. The applicability of theproposed CsPbBr3 NFs for use under various environmental conditions is verified by analyzing theirstability in response to changes in temperature, moisture level and pH, which are critical drawbacks ofCsPbBr3 NCs. Gaseous ammonia (9 mg L 1) in an N2 carrier is subsequently detected using thephotoluminescence (PL) properties of the CsPbBr3 NFs. In addition, an ammonia concentration of 100.0mg L 1 in an aqueous solution can be detected using the PL intensity. Therefore, we believe that theproposed CsPbBr3 NFs show significant promise for use in detection sensors to assess the extent of fooddecay, allowing customers to purchase fresh agricultural products more safely.

Optical Trapping Forces Depending on Size of Dielectric Polystyrene Microspheres

박범준,Eric M. Furst 한국고분자학회 2013 Macromolecular Research Vol.21 No.11

위암발생과 관련된 Helicobacter pylori의 병원성 인자에 대한 동정

박범준,박수정,김영열,임중연,서길주,이상민,박상익 국립보건연구원 2000 국립보건원보 Vol.37 No.-

Demonstration of unusual colloidal interaction via optical laser tweezers: Dipole-induced dipole attraction due to counterion rearrangements around colloidal particles near the oil-water interface

박범준 한국공업화학회 2020 한국공업화학회 연구논문 초록집 Vol.2020 No.-

We use the optical laser tweezer apparatus to investigate the dipoleinduced dipole attraction between colloidal particles that has not been reported yet. We postulate that the perpendicular electric dipole formed around a particle at an oil-water interface leads to rearrangements of surrounding counterions in the aqueous phase. The rearrangements can cause unexpected attractions when another particle dispersed in water approaches the interface-trapped particle nearby. We quantitatively measure the dipole-induced dipole electrostatic interactions and the critical time of counterion migration for generating the attractive force that can overcome the double layer interaction as well as the thermal fluctuation.