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Lipoxygenase 및 Kunitz Trypsin Inhibitor 단백질 결핍 콩 계통 육성
김명식 ( Myung Sik Kim ),성미경 ( Mi Kyung Sung ),서상배 ( Sang Bae Seo ),김경록 ( Kyung Roc Kim ),이경자 ( Kyoung Ja Lee ),박모세 ( Mo Se Park ),정종일 ( Jong Il Chung ) 한국콩연구회 2008 韓國콩硏究會誌 Vol.25 No.1
성숙한 콩 종실에 존재하는 성분 중 비린내를 일으키는 lipoxygenase와 단백질의 분해를 방해하는 효소인 Kunitz trypsin inhibitor (KTI) 단백질이 결핍된 녹색자엽 검정종피 및 노란종피 계통을 육성하기 위해여 진행된 연구에서 얻어진 결과는 다음과 같다. P1 x P2 및 P3 x P4의 교배로부터 얻어진 F2 종자에서 lipoxygenase-2, 3 및 KTI 단백질이 결핍된 종자가 선발되었다. 계통육종법으로 세대를 진전시킨 결과 녹색자엽이고 검정 종피색을 가진 lipoxygenase 및 KTI 단백질 결핍 계통은 생육습성이 직립형이고 신육형은 유한신육형이며 경장은 약 50cm로 나타났다. 종실 크기는 백립중이 약 23.2 g 정도이고 종실모양은 편구형이며 6월 초순 파종시 성숙기가 10월 초순경이었다. 노란종피를 가진 lipoxygenase 및 KTI 단백질 결핍 계통은 배축색이 중간 정도의 자색이며 신육형은 유한신육형이었다. 생육습성은 직립형이고 경장은 약 54cm로 중간 정도이며, 화색은 담자색이며 백립중이 약 24g 정도이고 종실모양은 구형이며 제색은 흑색이었다. Lipoxygenase and Kunitz trypsin inhibitor proteins in mature soybean seed are well known as major anti-nutritional factors. Lipoxygenase is responsible for the beany flavor and soybean Kunitz trypsin inhibitor (KTI) protein is responsible for the inferior nutritional quality of unheated or incompletely heated soybean meal. Anthocyanins from black soybean seed coat are known to have many pharmaceutical effects. The objective of this study was to breed soybean genotype of black coated-seed lacking lipoxygenase-2,3 and KTI protein and to breed soybean genotype of yellow coated-seed lacking lipoxygenase-2,3 and KTI protein. Two breeding populations were developed using four different parents. In F2 seed generations of both populations, F2 seeds deficient in lipoxygenase-2,3 and KTI protein were selected by SDS-PAGE. Selected F2 seeds were planted to advance F2 plant. Traits for seed coat and mature embryo colors were observed on the F3 seeds harvested from each F2 plant. F3 lines were advanced from F3 seeds selected. Plant type, height, and maturity date for each F3 plant within line were observed. After single plant harvesting, seed color and absence of lipoxygenase-2,3 and KTI protein were confirmed on random F4 seed of each F3 plant. Selected lines were advanced to the F6 generation. Maturity date, stem type, seed coat and cotyledon color, seed weight were observed on the new soybean lines with absence of lipoxygenase-2,3 and KTI protein.