Effects of Biodegradable-Film Mulch and Residue on the Growth and Yield of Pepper and Onion Cultivated under a Two-year Crop Rotation Scheme

박도원,최현석 한국원예학회 2025 원예과학기술지 Vol.43 No.3

This study was conducted to evaluate the effects of biodegradable (BD) films mulched in crop rotation fields, specifically pepper from April to September and onion from October to May, as well as the effects of the film residue on soil mineral nutrition and crop growth outcomes in South Korea. Five treatments were used: non-mulching (NM), polyethylene (PE) film mulch, and three BD film mulches (referred to as F, H, and E). Film F was made using polybutylene adipate-co-terephthalate (PBAT) and polylactic acid (PLA); film H was made using PBAT+PLA; and film E was made using PLA+starch. The pepper field showed greater levels of degradation of all BD films, in particular for the H and F films, compared to the films in the onion field under exposure to excessively warm temperatures and high rainfall amounts during the cultivation period. The F film-mulched pepper plots had the lowest soil moisture level of ‒47.0 kpa on August 1, 2023 owing to high biodegradability, and the values for the onion plots remarkably in April of 2024. Seasonal soil electrical conductivity decreased in both the pepper and onion plots, with significantly higher levels in the mulch plots than in the NM plots in the pepper field at 0 and 30 days after transplanting (DAT). In most cases, no significant differences were observed in the soil mineral nutrient levels between the treated pepper and onion plots. The stem height increased in peppers grown under PE film mulching at 60 DAT and under F film mulching at 120 DAT. The number of pepper leaves increased with the BD mulching treatments at 60 and 90 DAT, with higher SPAD readings observed at 60 DAT. The greatest stem diameter and leaf fresh weight values were observed at 180 DAT in the E film-mulched onions. All mulch treatments increased the number of pepper fruits. The H film mulching treatment produced a large number of bulbs and resulted in the highest yield of 61.1 ton ha-1, followed by mulching with the E (59.4 ton), PE (48.5 ton), F (43.3 ton), and NM (34.0 ton) films. Breakdown of BD films, particularly the F film, occurred on topsoil with considerable disintegration at a depth of 10 cm in the onion field after the completion of one crop rotation cycle. Adverse effects from the remaining BD residue were not observed for the subsequent period of onion growth, and the H and E films were recommended to preserve soil health and improve crop productivity of warm- and cool-season vegetables.

도덕교육에서 포스트휴먼 질적연구의 활용

박도원 한국도덕윤리과교육학회 2024 한국도덕윤리과교육학회 학술대회 자료집 Vol.2024 No.8

Characteristics of lytic phage vB_ EcoM‑ECP26 and reduction of shiga‑toxin producing Escherichia coli on produce romaine

박도원,김가연,이영덕,박종현 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.2

Foodborne Shiga toxin-producing Escherichia coli (STEC) cause severe diarrhea and hemolytic uremic syndrome (HUS) in humans. However, traditional methods for STEC sterilization are difficult to apply to fresh food. To control the pathogen, phage infecting E. coli O157:H7 were isolated and characterized. The isolated phage vB_EcoM-ECP26 had an icosahedral head and a contractile tail, and was classified as belonging to the Myoviridae family. The phage showed a broad host range against STEC and exhibited a large burst size of 1914 PFU/cell. The phage was highly stable at high temperatures (65 °C) and wide ranges of pH (4–10). The genome of vB_EcoM-ECP26 consists of 136,993 nucleotides, 214 open reading frames, and does not contain lysogenicity-related genes. Phylogenetic analysis showed that vB_ EcoM-ECP26 is a V5-like species. STEC O157 growth was inhibited by vB_EcoM-ECP26 for 8 h. Furthermore, this phage not only significantly decreased the STEC population (p < 0.05), but also persisted in fresh lettuce at 4 °C for 5 days. Therefore, these results reveal that the novel lytic phage vB_EcoM-ECP26 could be a useful agent for the control of foodborne STEC.

Differences in presenting alibi and evidence by day and time

박도원,박지선 한국법심리학회 2018 한국심리학회지: 법 Vol.9 No.3

The current study aimed to investigate the frequency differences in presentation of alibi and evidence by factors ̒day̓ and ̒time̓. We analyzed the frequencies of presenting alibis, physical evidence and witness evidence, and investigated the base rate by day (weekday / weekend) and time. A total of 282 respondents participated in this study through self-report online questionnaires. Participants were randomly assigned to one of 8 conditions that consisted of either the day (Tuesday / Saturday) and the time (3:00 / 9:00 / 15:00 / 21:00), and then they were asked to generate their alibis (location), physical evidence, and witness evidence to prove their innocence from mock robbery that they did not commit. Chi-square test was utilized to verify differences in the evidence reported by participants for certain day and time. In addition, binary logistic regression analysis was used in order to investigate the effect of day and time on the evidence. As a result, the day influenced whether family members witness evidence was reported and the time influenced all types of physical and witness evidence. In other words, people are most likely to report the witness evidence with the weakest reliability on weekend, and believable physical and witness evidence were least often reported at 3am in which crimes are most frequent. This study shows that a perfect alibi and evidence to prove innocence in the investigation process may not be possible. Finally, the limitations of the present study and suggestions of subsequent study were discussed.

박테리오파지의 살균소독제 응용을 위한 안전성 평가

박도원,이영덕,박종현 한국식품과학회 2020 한국식품과학회지 Vol.52 No.1

To evaluate the safety of bacteriophages for application of sanitizer, endotoxin content and cell cytotoxicity of two Escherichia coli and four Staphylococcus aureus phages were determined. Endotoxin ratio was determined by the Limulus amebocyte lysate (LAL) assay as a test for representative biological endotoxin content. The average endotoxin average content of the 9 log PFU/mL lysate was 18.6 EU/mL and that of the 10 log PFU/mL lysate was 5.9 EU/mL, suggesting that the phage lysate was not suitable for clinical applications, but suitable for food pathogen control applications. To confirm the cell cytotoxicity of the phage lysates, MTT assay was performed using Raw 264.7 cells treated with 9 log PFU/mL phages. Results of the assay indicated that the phage lysates did not significantly decrease the cell viability (p>0.05). These results indicated that bacteriophages would be suitable as a food safety sanitizer.

Prediction and identification of new type holin protein of Escherichia coli phage ECP26

박도원,이종훈,박종현 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.7

Bacteriophages (phages) infecting specifically target bacteria utilize a unique lysis module known as the holin-endolysin cassette to release progeny. Studies on the phage lytic proteins could contribute to the development of alternatives to antibiotics. Here, we predicted and identified the holin protein of rV5-like phage ECP26 for increasing lytic activity of the phage endolysin. In silico analysis revealed that open reading frame 151 (ORF151) of ECP26 contained two transmembrane domains. Co-expression of endolysin with ORF151 resulted in the cell lysis of Escherichia coli, suggesting that ORF151 protein functioned as the holin that disrupted the cytoplasmic membrane. The putative holin showed a high amino acid homology by more than 80% to the predicted holins of rV5-like phages. Therefore, the holin protein would be helpful for developing efficient lysis strategies with endolysin against gram-negative E. coli.

Characteristics for phage-encoded cell wall hydrolase of LysSAP27 to reduce staphylococcal food poisoning

박도원,이영덕,박종현 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.5

To reduce staphylococcal food poisoning (SFP),a phage-encoded cell wall hydrolase called endolysin hasemerged as an attractive antibacterial agent. In this study,the Staphylococcus aureus infecting phage vB_SauSSAP27(/SAP27) was isolated from sewage and characterizedmorphologically and genetically. /SAP27 wasidentified as Siphoviridae temperate phage, with a genomeof 43 kbp. A /SAP27 endolysin named LysSAP27 wasproduced recombinantly in Escherichia coli. LysSAP27exhibited the highest activity at neutral pH and a temperatureof 30 C, and its lytic activity was upregulated bycalcium ions. Following optimization of the enzymaticconditions, LysSAP27 was applied to S. aureus-contaminatedmilk. Treatment with 2 lM LysSAP27 led to a significantbactericidal effect, corresponding to a reduction inbacterial titer by 2.8 log CFU/mL within 1 h and 3.4 logCFU/mL within 2 h. Therefore, LysSAP27 could be usedas an effective antimicrobial agent to prevent SFP in food.

대학교 라카이브(Larchive) 인식 조사 및 실무 운영 방안

박도원,오효정,Park, Do-Won,Oh, Hyo-Jung 한국기록학회 2023 기록학연구 Vol.- No.77

The cooperation between archives and libraries is necessary for the management of limited operational space and the improvement of work efficiency. "Larchive" is one of the model of cooperation between libraries and archives, so it can be an alternative plan for institutions that face difficulties in collaborating through "Larchiveum" - growing model of cooperation between libraries, archives, and museums. This study presents the recognition of Larchive to university archivists and librarians, and suggests a practical operation plan for cooperation between the archive and library. As a result, "Larchive" was relatively less aware of archivists and librarians, but in the practical point of view, respondents were fully aware of the need for cooperation between archives and libraries. In particular, Larchive was presented as a rational alternative model for both of the groups. And the need for material cooperation can be confirmed through the recognition survey, and the improvement plan for business cooperation can be confirmed through the FGI. Some prerequisites are proposed such as securing a collaborative workplace, assignment of budget and manpower. Through the results, this study presented practical operational plans for organizational cooperation in the form of Larchive, focusing on the perspectives of "teaching and learning support", "research support services", "curation services", "collection and management of school history data", "cooperation for evaluation", and drew discussion points.

도덕교육에서 포스트휴먼 질적연구의 활용

박도원 한국도덕윤리과교육학회 2024 도덕윤리과교육 Vol.- No.85

학제적 접근을 취하는 도덕교육에서 다양한 학문 분야의 상호 교류와 소통이 이루어지는 연구 방법을 활용해야 할 필요성이 제시됨에 따라, 본 연구에서는 최근 국내외에서 주목받고 있는 포스트휴먼 질적연구를 도덕교육에서 활용할 필요성을 고찰하고 활용 방향을 제안하였다. 포스트휴먼 질적연구는 탈인간중심주의를 표방하는 포스트휴머니즘에 기반하여 개발된 연구방법론이다. 포스트휴머니즘은 근대적 주체성에 대한 반성 하에 행위성을 가진 인간과 비인간 존재들 간의 얽힘과 상호작용의 관점에서 세계를 인식하며 책임의 윤리를 강조한다. 포스트휴먼 질적연구는 이를 반영하는 한편 기존 질적연구의 절차적 한계를 보완하고자 한다. 도덕교육에서 포스트휴먼 질적연구를 활용한다면 기존의 연구 방법을 통해 실현하지 못했던 확장적 탐구를 실행할 수 있을 것이라 기대된다. 또한 새로운 연구 방법에 개방적으로 접근함으로써 도덕교육 연구방법론의 균형적 발전에 기여하고, 다양한 학문 분야와의 교류와 소통을 촉진할 수 있다.

임시의정원 관련 기록물의 정리 현황과 재정리 방향

박도원 한국기록학회 2022 기록학연구 Vol.- No.73

본고에서는 국회도서관이 소장하고 있는 임시의정원 관련 기록물의정리 현황을 살펴보고 기록물 정리의 원칙을 적용하여 재정리 방향을제시하였다. 임시의정원은 기록물 관리 규정을 가지고 있었고, 그에 따라 기록물을 생산하고 보관했다. 그러나 어느 시점에 기록물의 원질서가 해체되어 보관되다가 1960년대에 국회도서관이 수집하여 관리하고 있다. 국회도서관은 수집한 기록물을 정리하면서 생산 당시의 기록관리 체계와 보관 과정에서 있을 수 있는 여러 가지 상황을 충분히 고려하지 못했다. 당시 국회도서관은 임시의정원의 질서를 따르지 않고 정리했다. 또한 정리 과정에서 기록물의 계층 개념을 적용하지 않았고, 출처주의와 원질서 존중의 원칙을 고려하지 않았다. 그 결과 기록물의 구조와맥락을 파악하기 어렵게 되었다. 이러한 문제점을 해결하고 기록물의 재정리 방안을 마련하기 위해서는 우선 기록물 정리 원칙에 따라 임시의정원 관련 기록물이 지니고있는 특징을 살펴보아야 한다. 첫째, 출처주의에 따라 기록물 조직·기능·산출물이 무엇인지 파악하여 기록물의 기록물건·기록물철, 생산자, 생산일자, 기록물 유형 등을 구분해야 한다. 둘째, 원질서 존중의원칙을 적용하여 기록물을 생산하여 보존하던 당시의 기록물 질서가어떠했는지를 파악해야 한다. 셋째, 기록물이 완전하게 성립하였는지효력을 갖추고 있는지를 검토해야 한다. 임시의정원 관련 기록물을 과거의 모습대로 온전하게 정리하는 것은 불가능하다. 다만 정리 현황과 재정리 방향을 검토함으로써 기록물의 내용·구조·맥락을 새롭게 파악하고 효과적으로 서비스할 수 있는토대를 만들 수 있을 것이다.