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페놀분해세균인 Pseudomonas sp. EL-04J로부터 Trichloroethylene 분해효소의 확인
박근태,김호성,손홍주,이건,박성훈,이상준,Park, Geun-Tae,Kim, Ho-Sung,Son, Hong-Ju,Lee, Gun,Park, Sung-Hoon,Lee, Sang-Jun 한국생명과학회 2002 생명과학회지 Vol.12 No.5
Pseudomonas sp. EL-041는 페놀로 적응된 활성오니로부터 분리되었으며, 페놀을 분해할 수 있는 동시에 trick-loroethylene(TCE)을 공동대사할 수 있는 세균이다. 본 연구에서는 Pseudomonas sp. EL-041에서 발현되는 trick-loroethylene 분해효소의 종류를 효소활성 측정 및 specific phenol oxygenase gene의 DNA sequencing을 통하여 조사하였으며, 그 결과 본 균주의 trichloroethylene 분해효소는 monooxygenase였으며, phenol hydroxylase로 추정되었다. Pseudomonas sp. EL-041 was previously isolated from phenol-acclimated activated sludge. This bacterium was capable of degrading phenol and cometabolizing trichloroethylene (TCE). In this study, we report the identification of trichloroethylene- degrading enzyme in Pseudomonas sp. EL-041 by the investigation of enzyme activity and DNA sequencing of specific phenol oxygenase gene. As the results of experiment, trichloroethylene-degrading enzyme in Pseudomonas sp. EL-041 was monooxygenase and suspected to phenol hydroxylase.
박근태,Seung U. Kim,최경철 대한암학회 2017 Cancer Research and Treatment Vol.49 No.1
Purpose Genetically engineered stem cells may be advantageous for gene therapy against various human cancers due to their inherent tumor-tropic properties. In this study, genetically engineered human neural stem cells (HB1.F3) expressing Escherichia coli cytosine deaminase (CD) (HB1.F3.CD) and human interferon-β (IFN-β) (HB1.F3.CD.IFN-β) were employed against lymph node–derived metastatic colorectal adenocarcinoma. Materials and Methods CD can convert a prodrug, 5-fluorocytosine (5-FC), to active 5-fluorouracil, which inhibits tumor growth through the inhibition of DNA synthesis, while IFN-β also strongly inhibits tumor growth by inducing the apoptotic process. In reverse transcription polymerase chain reaction analysis, we confirmed that HB1.F3.CD cells expressed the CD gene and HB1.F3.CD.IFN-β cells expressed both CD and IFN-β genes. Results In results of a modified trans-well migration assay, HB1.F3.CD and HB1.F3.CD.IFN-β cells selectively migrated toward SW-620, human lymph node–derived metastatic colorectal adenocarcinoma cells. The viability of SW-620 cells was significantly reduced when co-cultured with HB1.F3.CD or HB1.F3.CD.IFN-β cells in the presence of 5-FC. In addition, it was found that the tumor-tropic properties of these engineered human neural stem cells (hNSCs) were attributed to chemoattractant molecules including stromal cell-derived factor 1, c-Kit, urokinase receptor, urokinase-type plasminogen activator, and C-C chemokine receptor type 2 secreted by SW-620 cells. In a xenograft mouse model, treatment with hNSC resulted in significantly inhibited growth of the tumor mass without virulent effects on the animals. Conclusion The current results indicate that engineered hNSCs and a prodrug treatment inhibited the growth of SW-620 cells. Therefore, hNSC therapy may be a clinically effective tool for the treatment of lymph node metastatic colorectal cancer.
Effects of Lactobacillus-fermented Ginger Stem on Salmonella-infected Broiler Chicks
박근태,오미향,심인숙,Jungok Nam,Kibbeum Ji,Jong-Kwon Han,지규만 한국생물공학회 2016 Biotechnology and Bioprocess Engineering Vol.21 No.2
Broiler salmonellosis is a major problem for poultry industry. Here, we supplemented broiler feed with 1% of ginger stems (GS) fermented with Lactobacillus paracasei and analyzed the effects on the resistance to Salmonella gallinarum. The chickens were divided into four dietary groups. The control group (C) received the basal diet, and the other chickens received the basal diet supplemented with 0.1% w/w L. paracasei ML-7 (L group), 0.1% ginger stem powder (GS group), or 0.2% fermented ginger stem (FGS group) for 21 days. The dietary groups were further split into two subgroups: one challenged with 1 × 105 CFU/mL S. gallinarum orally administered in 1 mL of saline from days 7 and 14, and one that received 1 mL of saline without bacteria. Both uninfected and S. gallinaruminfected broilers fed with fermented GS (FGS) significantly increased body weight and feed intake, and had lower mortality compared to relative control groups. Furthermore, dietary FGS decreased cecal, Salmonella spp. counts and serum IgA and IgG levels. These results indicate that FGS prevented S. gallinarum colonization and promoted weight gain in broilers, suggesting that FGS supplementation can be effectively used as a replacement of antibiotic growth promoters to prevent Salmonella infection.
박근태,안창환,강병택,강지훈,정의배,양만표 한국임상수의학회 2017 한국임상수의학회지 Vol.34 No.3
Fucoidan, a cell wall polysaccharide found in the brown seaweed, is reported to have broad-spectrum biological activities. The objectives of this study were to examine the effect of fucoidan on prostaglandin E2 (PGE2) and cyclooxygenase-2 (COX-2) expression in lipopolysaccharide (LPS)-stimulated porcine peripheral blood mononuclear cells (PBMCs) and to determine whether these effects are involved in Akt activation. The levels of PGE2 production in the culture supernatants from PBMCs were determined by the enzyme-linked immunosorbent assay (ELISA) kit and the levels of COX-2 mRNA were measured by real time polymerase chain reaction (RT-PCR). Akt activity was determined by Western blot analysis. Fucoidan in LPS-naïve PBMCs has no effect on PGE2 production and COX-2 mRNA expression. Furthermore, fucoidan does not affect Akt activation in LPS- naïve PBMCs. However, PGE2 production and COX-2 mRNA expression on PBMCs were remarkably enhanced by LPS stimulation. Akt activity was also increased by LPS. Increasing effects of PGE2 production and COX-2 mRNA expression in PBMCs induced by LPS were suppressed by addition of fucoidan. In addition, fucoidan reduced an increase in Akt activity in LPSstimulated PBMCs. These results suggested that fucoidan exerts potent anti-inflammatory properties by suppression of PGE2 production, COX-2 mRNA expression and Akt activation in LPS-stimulated PBMCs.
박근태 한국신석기학회 2018 한국신석기연구 Vol.- No.36
A Study on Earthenware Bowl with Raised Pattern in Jeju Island and the Southern Coast Park keun tae (Jeju Archaeological Institute Research Director) Earthenware Bowl with Raised Pattern is a Neolithic pottery that was used around the southern provinces of the Korean Peninsula early stage in inland regions, including the Jeju Island, Gageodo from the donghaeIs being identified. More than 15 sites have been distributed in Jeju Island since 1989 when it was reported in the area of Danae-myeon near Gosan-ri. I understood that the pottery vessels of Jeju Island were introduced through exchanges with historic sites in the islands off the southwestern coast. In this article, we classified the pottery vessels of Jeju Island by type, and then tried to divide them by year after comparing them with various historical sites around the southern coast. Then, they tried to find out the cultural aspect of the early Jeju Island by identifying the aspect of the stone tools and the well-known water bodies. As a result, southeastern coastal areas rather than traditional exchanges with the Southwestern coast area, Earthenware Bowl with Raised Pattern in the influx of culture and artifacts have been identified that can be compared with just geographically.Dongnam-gu, as well as exchanges with the west coast islands near coastal areas and judged to be exchanges or must be taken into account the influx. In addition, the stone tools and the poorness of the relics are assumed to have an environmental impact at an early stage
박근태,김현철,이택승 대한화학회 2021 Bulletin of the Korean Chemical Society Vol.42 No.1
To detect nitroaromatic picric acid (PA) in water, polyfluorene (PF) encapsulated melamine-formaldehyde microcapsules (PF@MFMs) were fabricated via in situ polymerizations. Because of the microcapsule structure, PF@MFMs were uniformly dispersed in water maintaining the blue fluorescence of PF in the capsules. The fluorescence intensity of PF@MFMs linearly decreased with increase in the PA concentrations with high limit of detection of 3.0???10?6 M. The addition of other nitroaromatic derivatives, which had structure similar to PA, or various ions did not affect the fluorescence of PF@MFMs, showing high specificity of the microcapsules toward PA.