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박근선,임종우,박채원,염민주,함승주 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.2
Avian influenza virus (AIV) causes acute infectious diseases of poultry, especially High pathogenic AIV (HPAIV) are cause of morbidity and mortality. Therefore, it is very important to take preventive measures through rapid detection of HPAIV for minimizing economic loss. In this study, we developed cell mimicking nanoparticles (CMPs) for a rapid detection of HPAIV and LPAIV via viral fusion mechanism. CMPs was polymeric nanoparticles constructed with sialic acid and FRET dye pairs, exposing FRET off signal responding to LPAIV and HPAIV activated by enzymatic cleavage at endosomal condition. CMPs exhibited accurate detection of various types of LPAIVs and HPAIVs in both cases with egg-cultured and fecal spiked specimen, showing superior sensitivity compared to rapid kit. The proposed strategy for mimicking viral infection can be a potent diagnostic assay for LPAIV and HPAIV, contributing to minimize economic loss by execution of preventative measures against the viral outbreaks.
박근선 한밭대학교 적정기술연구소 2020 적정기술 Vol.12 No.2
아프리카는 그동안 국제사회의 대규모 원조에도 불구하고 여전히 빈곤문제에 대한 해 결의 실마리를 찾지 못하고 있다. 이러한 가운데 최근 들어 지역주민의 삶에 직접 다가가 서 해당 지역사회의 빈곤 해소에 최적화되도록 개발된 적정기술이라는 새로운 원조 패러 다임이 등장하여 해당지역의 자연/지리적, 환경, 사회/문화적 특성에 이르기까지 여러가 지 제반요인을 함께 아우르는 기술 지원을 추구하는 움직임이 커지고 있다. 본 글에서는 필자가 그 동안 차드에서 실행한 적정기술 프로젝트에 대해서 소개하고자 한다.
정은지,박근선,김진영,박채원,이소정,함승주 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.2
Unpredictable pandemic caused by viruses raised the need for rapid detection which plays a crucial role in the efficient management on site. Herein, we designed colorimetric immunoassay for influenza virus detection with layer-by-layer signal amplification based on avidin & biotinylated Gold Nanoparticles(bGNPs) complex. To accomplish this system, we identified interaction with bGNPs and virus captured by antibody on a microtiter plate. In the presence of virus, red color appears and the visibility is proportionate to the number of Avidin-bGNPs. And peroxidase mimicking property of GNPs that catalyzes the chromogenic substrates with H2O2 offers another amplification that leads to enhanced intensity and the color becomes blue. Finally, we verified the naked-eye detection of influenza virus within 2h, shorter than that of ELISA. Thus, the designed system not only suggests alternative way to conventional colorimetric immunoassays but serve as promising tool for field diagnostic tests.
정수현,박채원,박근선,이소정,임종우,함승주 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.2
MicroRNAs (miRNAs) are promising diagnostic biomarkers, involved in gene regulation process associated with various diseases. Particularly, miRNA from tumor-derived exosomes play pivotal role in noninvasive cancer diagnosis, since tumor exosomes are highly enriched in body fluids. However, current methods of exosomal miRNA detection require substantial amount of time and expense. Here, we propose detection system consisting of two major procedures; membrane fusion and non-enzymatic target recycling signal amplification. Membrane fusion is induced by specific binding between Influenza virus-derived Hemagglutinin (HA) proteins on fusogenic vesicle and sialic acid on exosome. Upon successful vesicle fusion, miR-10b-activated DNA molecular machine generates amplified fluorescence signal by recycling target miRNA, contributing to the enhanced sensitivity. Therefore, the proposed method is expected to be remarkably time-saving and cost-cutting miRNA detection technique.
문예솔,이소정,박근선,김진영,박채원,함승주 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.2
We propose a plasmonic immunoassay based on a target specific organic-inorganic hybrid nanoparticle with an antibody-functionalized glass substrate. The hybrid nanoparticle (Gold-somes) encapsulates gold nanoparticles (GNPs) which are used to amplify the detection signal. In the presence of the virus, the glass substrate will capture the target, and viral fusion of Gold-somes will follow. During the viral fusion, encapsulated GNPs will escape the probe. With the addition of reducing agents, freed GNPs will form aggregates, resulting in a color change with respect to a peak shift. We conducted experiments to synthesize the hybrid nanoparticles (Gold-somes) and to optimize the reaction environment for the glass substrate. Eventually, we expect our plasmonic immunoassay platform be applied for point-of-care testing via colorimetric virion detection.