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RISS 인기검색어
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- 저자 : 박귀성 ( Kwi Sung Park ) (검색결과 6 건)
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박은하(Eun-Ha Park),김주한(Ju-Han Kim),김재훈(Jae-Hun Kim),김윤형(Yoon-Hyoung Kim),한상옥(Sang-Ok Han),금영범(Young-Bum Keum),정귀성(Kwi-Seong Jeong),고형진(Haeng-Zin Ko) 대한전기학회 2009 대한전기학회 학술대회 논문집 Vol.2009 No.7
본 연구에서는 수소 연료전지의 엔드플레이트와 외부 연결 부분의 금속을 진동시험기를 이용하여 접촉저항을 측정하였다. 수소 연료전지의 집접판에서의 전류밀도의 변화와 온도 분포의 변화를 비교해 볼 수 있었으며, 접촉저항을 측정 할 수 있었다. 진동시험기는 5∼50 ㎐의 범위까지 설계하였으며 진동에 대한 접촉저항의 변화도 확인 할 수 있었다.
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Multiplex RT-PCR 검사를 이용한 호흡기 바이러스 검출에서 비인두흡인법과 비면봉법의 비교
박준수 ( Joon Soo Park ),최영진 ( Young Jin Choi ),남해선 ( Hae Son Nam ),김용배 ( Yong Bae Kim ),박귀성 ( Kwi Sung Park ) 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 2009 소아알레르기 및 호흡기학회지 Vol.19 No.4
목적: 호흡기 바이러스를 정확히 검출하기 위하여 흔히 비인두흡인법을 사용하고 있으나, 비면봉법은 비인두흡입법보다 덜 아프고 편리하다. 최근 항원검출법이나 배양으로 검사를 할 때 두 검체간의 비교에서 비면봉법이 비인두흡인법만큼 검출률이 우수하다는 보고가 있었다. 본 연구에서는 소아에서 Multiplex RT-PCR 검사로 호흡기 바이러스를 검출할 때 비인두흡인법과 비면봉법간의 바이러스 검출률의 차이를 비교하고자 하였다. 방법: 2008년 l2월부터 2009년 6월까지 순천향대학교 천안병원 소아청소년과에 급성 호흡기 감염으로 입원한 소아 250명으로부터 비인두흡인법과 비면봉법으로 검체 250세트를 얻어 12개의 바이러스에 대한 두 검체간 multiplex RT-PCR검사 결과의 일치도를 구하고 민감도를 비교하였다. 결과: 대상 환아 들의 중간연령은 1.3년(생후 20일부터 16.5세)이었고, 대부분(288명 91.2%) 5세 미만이었다. 비인두흡인법과 비면봉법에서 바이러스 검사 결과의 일치도는 파라인플루엔자 3형은 우수 (COhen`s kappa>0.8)하였고 라이노바이러스 A, RSV A, RSV B는 충분(0.6-0.8)하였다. 아데노바이러스와 메타뉴모바이러스의 일치도는 미약(0.4 to 0.6)하였고 A형 인플루엔자 바이러스는 불량(poor<0.4)하였다. 바이러스 전체에 대한 검출률은 비인두흡인법에서 0.96(CI;0.92-0.98)로 비면봉법에서의 0.59(0.51-0.66)보다 의미 있게 높았다.(P<0.05) 결론: 입원한 환자의 경우에는 정확한 검사를 위해 비인두흡인법을 시행해야 하겠다. 비면봉법은 개원가나 외래 그리고 대규모 역학조사에서 제한하여 사용될 수 있겠으나 검체 방법에 따른 검출률의 차이를 고려할 때 A형 인플루엔자 바이러스,메타뉴모바이러스 그리고 아데노바이러스 경우에는 결과를 해석할 때 보다 세심한 주의가 필요하다. Purpose: Although the nasopharyngeal aspirate (NPA) is more commonly used because of relatively higher accuracy, the nasal swab (NS) is a less painful and easier method than NPA. A few recent reports have shown that NS is more effective than NPA for the detection of respiratory virus using immunofluorescence or viral culture. The objective of the present study was to compare the results of NPA and NS sampling specimens in children for respiratory viruses detection using multiplex RT-PCR. Methods: From December 2008 to June 2009 Paired NPA and NS specimens were collected from 250 children admitted with symptoms of acute respiratory infections at the Department of Pediatrics, Soonchunhyang University Cheonan Hospital, Cheonan, Korea. The sensitivity and agreement of virus detection between NPA and NS using multiplex RT-PCR were compared and analyzed. Results: The median age of the subjects was 1.3 years (range, 20 days to 16.5 years), and 228 patients (91.2%) were under the age of 5 years. The agreement of virus detection between NPA and NS was excellent (Cohen`s kappa >0.8) for parainfluenza virus type 3 or substantial (0.6 to 0.8) for rhinovirus A, RSV A and RSV B, moderate (0.4 to 0.6) for adenovirus and metapneumovirus and poor (<0.4) for influenza A. The overall sensitivity of detection of respiratory viruses was significantly higher in NPA (0.96) than in NS (0.59, P<0.05). Conclusion: We recommend NPA may be more accurate specimen for detection of respiratory viruses in hospitalized children. NS might be used in limited cases at a office setting or for larger epidemiological studies. However, results obtained from NS for influenza virus type A, metapneumovirus and adenovirus, should be interpreted carefully. [Pediatr Allergy Respir Dis(Korea) 2009;19:365-373]
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한국인집단의 D14S299 유전자좌내 microsatellite의 다형현상
박귀성,강혜자,안광숙 대전대학교 기초과학연구소 2000 自然科學 Vol.11 No.1
The allele frequency of D14S299 microsatellite locus was analyzed in 242 normal blood from Korean population. Using the polymerase chain reaction and polyacrylamide gel electrophoresis. we examined the allele and genotype frequency. The size of 0145299 PCR fragments ranged from 299 bp to 331 bp. The most frequent allele in the Korean subjects was allele 30 (44.9%) followed by allele 31 (25.4%), allele 32 (12.4%), and minor alleles (17.3%). The heterozygosity of D14S299 was 0.710. The polymorphism information content (PIC) and power of discrimination (POE) were 0.67 and 0.880, respectively in the present study. The genotype distribution and allele frequency of D14S299 microsatellite in the Korean subjects was similar to those of Japanese and Chinese populations including in the same Asian Population.
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한국인 집단에서 D14S299 및 Cellular Stress Response(CSR)유전자의 Microsatellite 다형현상
류성호,박귀성,안광숙 한국유전학회 2002 Genes & Genomics Vol.24 No.2
Allele frequencies at microsatellite loci D14S299 and CSR were analysed by polymerase chain reaction from Korean subjects. According to repeat number of microsatellite, nine alleles were identified at D14S299 locus and PCR amplified fragments ranged from 299 to 331 bps in size. The most frequent allele in this study was allele 30 (44.9%) followed by allele 31 (25.4%) and few more alleles. By the results of statistical analysis, the heterozygosity of D14S299 was 0.710, and the polymorphism information content (PIC) and the power of discrimination (POD) were 0.670 and 0.880, respectively. The genotype distribution and allele frequency of D14S299 microsatellite in Korean population were similar to those of Japanese and Chinese populations. Genes involved in cellular stress response (CSR) possess various numbers of dinucleotide (CA) repeats. Frequencies of two most commonly occurring alleles, a2 and a3, were 31.2% and 31.5%, respectively. Allele frequencies agreed with the Hardy-Weinberg expectation. The observed heterozygosity was 0.69, and the values of PIC and POD were 0.730 and 0.866, respectively. We found D14S299 and CSR were genetically polymorphic in the Korean population and useful microsatellite markers for forensic practices.
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입원한 소아에서 노로바이러스 급성 위장염의 임상 특성 및 유전형 분석 : 로타바이러스의 임상 특성과의 비교
송준환,선동신,박준수,최영진,남해선,김용배,정은혜,백경아,박귀성 대한감염학회 2010 감염과 화학요법 Vol.42 No.3
Background: Norovirus is one of the most prevalent pathogens causing acute gastroenteritis in children. We compared the clinical features of noroviral gastroenteritis to those of rotaviral gastroenteritis and analyzed the noroviruses' genotype frequencies. Materials and Methods: Stool samples were obtained form 433 children hospitalized with acute gastroenteritis from May 2008 through February 2009 at Soonchunhyang University Cheonan Hospital and examined for the presence of norovirus or rotavirus. We then analyzed the clinical features of noroviral gastroenteritis in comparison with rotaviral gastroenteritis and observed the capsid protein gene sequences from the isolated norovirus for genotyping. Results: Norovirus was isolated from 69 patients (16.4%) and rotavirus from 49 patients (11.6%). The noroviral gastroenteritis patients experienced vomiting (77.4%), diarrhea (73.2%), and respiratory symptoms (53.6%); the rotaviral gastroenteritis patients experienced diarrhea (71.4%), dehydration (69.3%), and vomiting (65.3%). Dehydration in patients with noroviral gastroenteritis (43.4%) was rare compared with rotavirus (69.3%) (P=0.008). The isolated norovirus belonged primarily to the GII.4 genogroup (85.5%). Our phylogenetic analysis of the GII.4 isolates revealed 3 clusters, including novel cluster C. Conclusions: Vomiting was the most common symptom in noroviral gastroenteritis patients. Dehydration in noroviral gastroenteritis patients was less common compared with rotavirus gastroenteritis patients. The majority of the norovirus strains isolated from children with acute gastroenteritis belonged to the GII.4 genogroup.
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