돼지 체세포 복제 수정란의 자가 사멸 분석

류지은,윤종택,Ryu, Ji-Eun,Yoon, Jong-Taek 한국수정란이식학회 2018 한국동물생명공학회지 Vol.33 No.3

The purpose of this study is to examined the electrofusion and activation conditions for the production of porcine somatic cell nuclear transfer (SCNT) embryos. In this study, immature oocytes were cultured in TCM-199 with and without hormones for 22 hours. Skin fibroblasts cells of porcine were transferred into the perivitelline space of enucleated in vitro matured oocytes. Cell fusion was performed with two different pulses that each one pulse (DC) of 1.1 kV/cm or 1.5 kV/cm for $30{\mu}sec$. After fusion subsequent activation were divided into three groups; non-treatment (control) and treatment with 2 mM 6-DMAP or $7.5{\mu}g/ml$ cytochalasin B for 4 hours. Transferred embryos were cultured in PZM-3 (Porcine Zygote Medium-3) in $5%\;CO_2$ and 95% air at $39^{\circ}C$ for 7 day. Apoptosis-related genes (Caspase-3, BCL-2, mTOR, and MMP-2) were analyzed by immunofluorescence staining. There was no significant difference between two different electrofusion stimuli in the cleavage rate; $64.9{\pm}4.8%$ in 1.1 kV/cm and $62.7{\pm}4.0%$ in 1.5 kV/cm. However, blastocyst formation rate (%) was significantly different among three different activation groups (no treatment, 2 mM 6-DMAP or $7.5{\mu}g/ml$ cytochalasin B) combined with electrofusion of 1.1 kV/cm. The blastocyst formation rate was $12.6{\pm}2.5$, $20.0{\pm}5.0$, and $34.9{\pm}4.3%$ in control, 2 mM 6-DMAP, and $7.5{\mu}g/ml$ cytochalasin B, respectively. Immunofluorescence data showed that expression levels of caspase-3 in SCNT embryos undeveloped to blastocyst stage were higher than those in the blastocyst stage embryos. Expression levels of Bcl-2 in blastocyst stage embryos were higher than those in the arrested SCNT embryos. These results showed that the combination of an electric pulse (1.1 kV/cm for $30{\mu}sec$) and $7.5{\mu}g/ml$ cytochalasin B treatment was effective for production of the porcine SCNT embryos.

도시의 공간 확장 및 형태적 특징에 따른 산림녹지의 변화 분석 - 서울, 대구를 중심으로 -

류지은,황진후,이준희,정혜인,이경일,최유영,주용언,성민준,장래익,성현찬,전성우,강진영,Ryu, Jieun,Hwang, Jinhoo,Lee, Junhee,Chung, Hye-In,Lee, Kyung-il,Choi, Yu-Young,Zhu, Yongyan,Sung, Min-Jun,Jang, Raeik,Sung, Hyun-Chan,Jeon, Seongwoo,Ka 대한원격탐사학회 2017 大韓遠隔探査學會誌 Vol.33 No.5

정부의 규제 및 정책은 무분별한 도시의 확장을 억제하는 중요한 수단이며, 이들의 기준에 따라 도시 공간의 확장으로 인하여 잠식되는 산림녹지의 증감이 뚜렷하기 때문에 본 연구에서는 우리나라에서 가장 대표적으로 고밀도 개발이 진행된 서울과 대구를 중심으로 '96년대부터 10년 단위로 도시의 확장에 따른 도시와 산림녹지의 변화를 정책 및 규제를 중심으로 해석하였다. 본 연구에서는 위성영상을 이용해 토지피복('96년, '06년대, '16년대)을 분류 후, 도시의 공간 확장 및 형태적 특징을 분석하기 위하여 도시확장강도지수(UEII)와 GUIDOS 프로그램을 이용하였으며, 이에 따른 산림녹지의 변화는 산림의 고도, 경사, 단일 산림패치의 면적을 기준으로 경년 변화를 분석하였다. 서울은 근교지역의 도시 공간 확장이 비교적 빠른 속도로 진행되고 있었으며, 이에 따른 산림 파편화가 발생하여 단일패치의 면적이 점차 감소하였다. 다만, 개발규제에 따라서 일정 기준 이상의 고도와 경사에서는 개발에 따른 면적감소가 거의 발생하지 않은 것으로 나타났으며, 규제 대상이 아닌 고도 및 경사의 산림은 등급 변화가 크게 나타났다. 대구는 '96년대 이후 도시 및 교외 지역이 느린 속도로 증가하였지만, '96년대부터 푸른숲가꾸기를 통해 산림녹지 면적이 크게 증가하는 양상이 나타났다. 이처럼 정부의 정책 및 규제에 따라서 개발에 따른 도시의 양적, 형태적 확장이 통제되고 산림녹지가 보존될 수 있기 때문에, 지속가능한 도시를 위하여 규제 및 정책을 적절히 활용해야 할 것이다. Government regulations and policies are important means of restraining the indiscreet expansion of urban areas. According to the standards from those means, it is clear that the fluctuation of forest green proportion encroached by the increase of urban space is obvious. In this study, we interpreted the changes of urban areas as well as the green ones owing to the urban expansion by the decades from 1996, with focusing on the cities of Seoul and Daegu highly developed in South Korea. The purpose of this study is to analyze the spatial expansion and morphological characteristics of urban land cover using not only satellite imageries (1996, 2006, 2016). but also the urban expansion intensity index (UEII) and GUIDOS program. Ultimately, this study is to compare the changes in the rate of forests due to urban expansions annually analyzed based on areas of forest elevation, slope, and the area of single forest patch. In Seoul, the expansion begun from urban space to suburban areas was comparatively rapid, which led the forest fragmentation and the gradual decline of the single patch. However, when it comes to DEM (Digital elevation model) and slope above a certain standard, by the development regulations, there was little decrease in area by anthropogenic developments. The city of Daegu has increased at a slow speed since 1996 in urban and suburban areas, whereas green forests have greatly increased through green forest conservation campaigns. In this way, as to the government policies and regulations, the quantitative and morphological expansion of cities owing to development could be controlled and forest spaces could be preserved as well. Therefore, regulations and policies by the government should be appropriately utilized for sustainable cities.

4년제 이공계 여대생의 진로장벽 인식과 진로타협 유형화 연구

류지은(Ji-eun Ryu),정진철(Jinchul Jeong) 한국진로교육학회 2016 진로교육연구 Vol.29 No.2

이 연구의 목적은 4년제 이공계 여대생의 진로타협을 유형화하고, 각 진로타협 유형과 진로장벽 인식 간의 관련성을 밝히는 데 있었다. 따라서 성역할, 사회적 지위, 흥미 등의 진로타협 요인 간 타협순서와 타협강도에 따라 몇 개의 군집으로 유형화하고, 각 유형별로 어떤 진로장벽 인식의 특성을 보이는지 확인하고자 하였다. 연구대상은 4년제 이공계 여대생으로 총 369부의 자료로 분석을 진행하였으며, 기술통계 분석, 집단 간 차이 분석, 잠재 집단분석을 실시하였다. 이 연구로부터 도출된 결론은 다음과 같다. 첫째, 우리나라 4년제 이공계 여대생들의 진로타협 유형은 일지향형, 수입지향형, 이상지향형, 일-가정균형지향형 등 4가지로 구분될 수 있으며, 각 진로타협 유형에는 서로 다른 타협순서와 타협강도의 양상이 반영되어 있다. 둘째, 일지향형은 진로장벽 인식 수준이 비교적 낮은 유형으로 구체적인 이공계 분야의 직업정보를 제공하여 현실적인 진로의사결정을 지원할 필요가 있. 다셋째, 수입지향형은 자기명확성 부족, 경제적 어려움, 흥미 부족을 높게 인식하는 유형으로 자신에 대한 이해를 높이고 관심 직업의 수입 정보를 중심으로 직업정보를 제공하는 등의 상담이 이루어져야 한. 다넷째, 이상지향형은 직업정보 부족과 미래 불안을 높게 인식하는 유형으로 진로상담 시 이공계 분야의 다양한 커리어 패스 정보를 제공할 필요가 있다. 다섯째, 일-가정균형지향형은 중요한 타인과의 갈등을 진로장벽으로 인식하는 유형으로 진로상담 시 진로의사결정의 주체성을 확립하고 갈등을 조율해 나갈 수 있도록 지원할 필요가 있. 다마지막으로 유형별 차이를 고려한 상담 접근의 시도, 일-가정 양립 문화 정착을 위한 방안 마련, 성역할, 지위, 흥미 등 진로 타협 요인에 대한 시대적 흐름을 반영한 고찰과 각각에 대한 세부요인 구명, 4년제 이공계 여대생의 진로타협 유형과 다양한 진로의사결정 관련 변인 간 관계 구명, 진로장벽 영향요인 구명, 전공별 진로장벽 인식과 진로타협 특성 비교의 필요성 등이 제안되었다. The purpose of this study was to categorize the career compromise of female students in science & engineering majors, and to identify relation between career compromise types and perception of career barriers. A total of 369 female students’ data were used for descriptive analysis, difference analysis, and latent class analysis. The conclusions of the study are as follows: First, career compromise type of female students in science and engineering majors could be categorized in four types, and each type reflects the aspect of distinctive compromise order and intensity. Second, ‘work-oriented type’ needs specific occupational information in science and engineering fields in order to practical career decision-making. Third, ‘income-oriented type’ needs opportunities to understand oneself, occupations, and specific income information. Fourth, ‘ideal-oriented type’ needs diverse career path information in science and engineering fields. Fifth, for ‘work-family balance-oriented type’, parents should to participate in career counseling. Suggestions based on the results of the study are as follows: First, diverse counseling approach should be attempted based on difference in career compromise type. Second, the policy should include the work-family balance and prevention of career discontinuity. Third, to categorize one’s career compromise type more correctly, particular factors of gender role, status, interest should be identified. Fourth, reviewing of career compromise factors should be implemented. Fifth, further research identifying relation between career compromise type and other career decision-making factors should be implemented.

복수 그래프의 조합을 통한 그래프 기반 PDF 암호시스템 개선 방법 제안

류지은(Jieun Ryu),강주성(Jusung Kang),염용진(Yongjin Yeom) 한국통신학회 2022 한국통신학회 학술대회논문집 Vol.2022 No.6

국내 외식식품의 무침류, 볶음류 및 국(탕)류에서 총 식이섬유 함량 분석

류지은 ( Ji-eun Ryu ),차승현 ( Seung-hyeon Cha ),이정윤 ( Jeong-youn Yi ),김영경 ( Young-kyung Kim ),김동호 ( Dong-ho Kim ),장금일 ( Keum-il Jang ) 한국식품영양학회 2017 韓國食品營養學會誌 Vol.30 No.3

본 연구에서는 6개의 지역(강원도, 경기도, 경상도, 서울, 전라도, 충청도)에서 채취한 외식식품 59종에 함유된 총 식이섬유 함량을 조사하였다. 총 식이섬유 함량은 AOAC 991.43분석법이 적용된 식이섬유 자동 분석기를 이용하여 분석하였다. 총 14종의 무침류 중에서 깻잎나물(4.33~6.24 g/100 g), 고구마줄기나물(3.16~5.07 g/100 g) 및 달래나물(3.70~4.99 g/100 g)이 다른 무침류에 비하여 높은 총 식이섬유 함량을 나타내었으며, 동일 무침류에서 지역간에 따른 차이는 나타나지 않았다(p>0.05). 그리고 13종의 볶음류 중에서 표고버섯볶음(4.77~6.66 g/100 g), 미역줄기볶음(4.16~7.47 g/100 g) 및 느타리버섯볶음(5.04~7.01 g/100 g)이 다른 볶음류에 비하여 높은 총 식이섬유를 나타내었는데, 표고버섯볶음과 미역줄기볶음이 지역간 차이를 나타내었다(p< 0.05). 표고버섯볶음은 경상도에서 가장 낮은 함량을 나타내었고, 미역줄기볶음은 강원도에서 가장 높은 함량을 나타내었으며, 다른 볶음류에서는 지역간 유의적인 차이를 나타내지 않았다(p >0.05). 국(탕)류에서는 시금치된장국(1.34~2.21 g/100 g), 닭고기육개장국(1.61~2.45 g/100 g), 오리탕(1.25~2.80 g/100 g) 및 조기매운탕(1.70~2.27 g/100 g)이 다른 국(탕)류에 비해 높은 총 식이섬유함량을 나타내었으며, 미소된장국을 제외한 지역간 유의적인 함량 차이를 나타내지 않았다(p>0.05). 따라서 본 연구에서는 위의 분석 결과를 토대로 소비자들에게 외식식품 중 총 식이섬유 함량 관련 정보를 제공함과 동시에 국가식품영양성분 database의 기초자료 구축을 위한 자료를 제공하였다고 생각된다. To aid in the development of a food nutrient database that provides the dietary fiber composition of eat-out Korean foods, we determined the total dietary fiber (TDF) content in the eat-out Korean foods Muchim, Bokkeum, and Guk (Tang) using a dietary fiber autoanalysis instrument. A total of 59 samples were collected from Gangwon-do, Gyeonggi-do, Gyeongsangdo, Seoul, Jeonla-do, and Chungcheong-do. First, among 14 samples of Muchim, the TDF content of Kkaennip namul, Goguma-julgi-namul, and Dallae-namul (4.33~6.24, 3.16~5.07 and 3.70~4.99 g/100 g, respectively) was higher than the other types of Muchim. There was no significant difference in TDF content of Muchim (p>0.05) among locations. Among 13 samples of Bokkeum, the TDF content of Pyogo-beoseot-bokkeum (4.77~6.66 g/100 g) and Miyeok-julgi-bokkeum (4.16~7.47 g/100 g) was higher than the other types of Bokkeum. The TDF content of Pyogo-beoseot-bokkeum in Gyeongsang-do was the lowest and the TDF content of Miyeok-julgi-bokkeum in Gangwon-do was the highest (p< 0.05). The TDF content of spinach soybean paste soup (1.34~2.21 g/100 g), Dakgogi-yukgaejang (1.61~2.45 g/100 g), duck stew (1.25~2.80 g/100 g) and spicy yellow croacker stew (1.70~2.27 g/100 g) were higher than the other types of Guk (Tang). There was no significant difference in TDF content of Guk (Tang) among locations (p >0.05).

대구지역 미용실고객의 라이프스타일이 재방문에 미치는 요인

류지은 ( Ji Eun Ryu ),오선영 ( Sun Young Oh ) 대한미용학회(구 대한미용과학회) 2015 대한미용학회지 Vol.11 No.2

The opportunity now exists for businesses with a differentiated strategy based on scientifically-derived customer preferences to improve customer quality of life through meeting their emotional needs. The aim of this study was to clarify the factors which may contribute to an increase in sales in order to attract new customers. To accomplish this, the current study assessed the factors influencing the return visits of customers to the Jung-gu beauty salon in Daegu by means of questionnaires completed between June 1, 2014 and August 31, 2014. These questionnaires contained questions pertaining to customers’ lifestyles. Frequency analysis, factor analysis, reliability analysis, person correlation analysis, dispersion analysis, cluster analysis, multiple regression analysis, and t-tests were all conducted using SPSS for Windows. Investigations regarding the subjects’ general characteristics were conducted on both men (42.4%) and women (57.6%). The factors influencing the propensity to revisit were significantly dependent on the subjects’ general characteristics including marital status, profession, self-employment, and a monthly income more 5 million, etc. More broadly stated, revisit rates and customer satisfaction were higher in professionals and high income earners. Therefore, the use of a beauty salon may be an important strategy in terms of industrial competitiveness. By increasing the quality of service and customer satisfaction, beauty salons may be more likely to create opportunities for customers to revisit.

PEO/LLZO 복합 고체전해질 기반 전고체 전지 실용화를 위한 대면적 시트 제조 기술 개발

류지은(Jieun Ryu),송영웅(Youngwoong Song),이효찬(Hyochan Lee),이정환(Junghwan Lee),노유정(Yoojung Noh),임진섭(Jinsub Lim),김재국(Jaekook Kim),김민영(Minyoung Kim) 한국전지학회 2023 한국전지학회지 Vol.3 No.2

All-solid-state batteries are attracting attention as next-generation secondary batteries for electric vehicles or ESS because they do not pose the risk of explosion or fire. However, most solid electrolytes have limitations in practical use due to the difficulty in expanding them to large areas. In this study, a composite solid electrolyte sheet combining an oxide-based inorganic solid electrolyte and a polymer solid electrolyte was manufactured through a tape casting process. As a result, it was confirmed that the manufactured composite solid electrolyte sheet had an ionic conductivity in the range of 3.33 to 4.45 × 10<SUP>-4</SUP> S/cm and a uniform thickness in the range of 39 to 41㎛. In addition, as a result of manufacturing Li/CSE/LFP cells and evaluating their cycle characteristics, they showed a high capacity retention rate of 82.33% in 50 cycles at 0.33 C and 70℃. Therefore, this study provides a practical strategy for fabricating all-solid-state batteries based on composite solid electrolytes.

LC-MS/MS를 이용한 채소류 및 서류의 클로로젠산 함량 조사

류지은(Ji-Eun Ryu),김운호(Woon-Ho Kim),배호정(Ho-Jeong Bae),정유정(You-Jung Jung),최영주(Young-Ju Choi),문경은(Kyeong-Eun Moon),최종철(Jong-Chul Choi),채경석(Kyung-Suk Chae),이진희(Jin-Hee Lee),도영숙(Young-Sook Do),최옥경(Ok-Kyung C 한국식품영양과학회 2020 한국식품영양과학회지 Vol.49 No.8

본 연구는 LC-MS/MS를 이용해 다소비 채소류 및 서류에 함유된 9종의 chlorogenic acid 이성질체를 분석하기 위하여 시료 추출 후 QuEChERS kit으로 정제하는 전처리법을 확립하였고, Syncronis C18 컬럼을 사용하여 30분 동안 분석하는 이동상 용매 조성과 9종의 이성질체의 정량 및 정성 이온을 선택하여 negative 모드에서 분석하는 기기 분석법을 확립하였다. 확립한 분석법은 AOAC 가이드라인에 따라 특이성, 직선성, 검출한계, 정량한계, 정확성, 정밀성을 확인하여 유효성을 검증하였다. 다소비 채소류 및 서류 43품목(48품종)의 chlorogenic acids 함량을 분석한 결과, 24품목(29품종)에 함유되었음을 확인하였다. 채소류의 경우 39품목(42품종) 중 22품목(25품종)에 chlorogenic acids가 함유되었으며(0.01±0.01~38.31±8.73 ㎎/100 g FW), 취나물, 케일, 미나리, 우엉, 상추, 브로콜리, 콜리플라워, 가지, 치커리, 도라지 순으로 함량이 높게 나타났다. 서류는 고구마(호박고구마, 밤고구마), 감자(로즈밸리, 수미), 마, 토란 등 4품목(6품종)을 분석하였는데, 고구마의 경우 밤고구마(1.31±0.52 ㎎/100 g FW)보다 호박고구마(1.42±0.39 ㎎/100 g FW)에, 감자의 경우 수미(0.09±0.03 ㎎/100 g FW)보다 로즈밸리(0.37±0.07 ㎎/100 g FW)에 chlorogenic acids 함량이 높게 나타났다. 또한 취나물, 미나리, 고구마, 감자를 대상으로 조리시간에 따른 chlorogenic acids 함량 변화를 확인한 결과, 취나물과 미나리는 30초 삶았을 때 가장 높은 함량을 나타내었고, 감자는 30분, 고구마는 20분쪘을 때 chlorogenic acids 함량이 높게 나타났다. 본 연구에서는 다소비 채소류 및 서류의 chlorogenic acids 함량과 조리시간에 따른 chlorogenic acids 함량 변화를 확인하여 식품 내 chlorogenic acids 함량에 관한 기초자료로 제공하고자 하였다. In this study, a sample preparation method and simultaneous determination method were developed to determine the chlorogenic acids content of frequently consumed vegetables, root and tuber crops in Korea by liquid chromatography coupled to mass spectrometry. The developed method was validated for its specificity, linearity, limit of detection, limit of quantification, recovery, accuracy, and precision based on the AOAC guidelines. Among the 39 items of vegetables, 22 items (0.01±0.01∼38.31±8.73 ㎎/100 g fresh weight (FW)) were quantified. The chlorogenic acids content of the vegetables was in the following order: chwinamul (38.31±8.73 ㎎/100 g FW), kale (22.93±0.75 ㎎/100 g FW), water dropwort (10.67±4.46 ㎎/100 g FW), burdock root (9.72±2.52 ㎎/100 g FW), red lettuce (leaf) (8.66±5.37 ㎎/100 g FW), broccoli (2.45±0.50 ㎎/100 g FW), and cauliflower (2.35±0.36 ㎎/100 g FW). Among the four items of root and tuber crops, sweet potato (1.42±0.39 ㎎/100 g FW) and potato (0.37±0.07 ㎎/100 g FW) were quantified. In addition, the chlorogenic acids content of chwinamul and water dropwort was highest when boiled for 30 seconds. Sweet potato steamed for 20 minutes and potato steamed for 30 minutes showed high chlorogenic acids contents.

시흥시 군자동 일대 산림 구조의 다양성 평가

류지은 ( Ji Eun Ryu ),강종현 ( Jong Hyun Kang ),이동근 ( Dong Kun Lee ) 한국환경복원기술학회(구 한국환경복원녹화기술학회) 2011 한국환경복원기술학회지 Vol.14 No.1

Habitats loss and fragmentation are major threats to biodiversity. There are various kinds of environmental assessment have been developed for various problems to solve. Yet, there are no well-developed methods for quantifying and predicting about biodiversity. To achieve a sustainable conservation for biodiversity, the structural diversity of forest must be assessed by proper indexes. This study aim to quantitatively assess the diversity of forest structure as habitats and results of the verification by bird survey for objective presentation of evidence. As a result of literature review, some indexes were selected as potential prediction tools for biodiversity; area of patch, area of core regions, shape of patch and average age of stand. The assessment results were estimated by monitoring of birds for accuracy verification and the results were almost in agreement with each others. But, 1 and 2 level of forests were showed ambiguous results. Certainly, this study was limited in some valuation indexes on landscape scale. Further studies should be considered that different environmental factors such as land use, disturbances by human and vegetation index. Also, we expect that the additional monitoring of birds should give rise to the result which is improved assessment results.

보호지역의 지정 및 관리를 위한 국립공원의 서식처 기능 평가 一생물종다양성과 서식처 가치에 기반하여一

류지은 ( Ryu Ji-eun ),최유영 ( Choi Yu-young ),전성우 ( Jeon Seong-woo ),성현찬 ( Sung Hyun-chan ) 한국환경복원기술학회(구 한국환경복원녹화기술학회) 2018 한국환경복원기술학회지 Vol.21 No.5

National parks are designated for the purpose of maintenance, conservation and utilization of different habitats. Therefore, it is necessary to select habitats of a high value as a protected area in order to balance conservation and development. However, the existing national park boundary adjustment and new designation criteria only focused on the endangered species and protected area, without proper evaluation of the habitat value of actual species. Therefore, this study aims to quantitatively evaluate habitat function in terms of biodiversity and habitat value, so that it can be referred to for the designation and boundary adjustment of national parks. We assessed species diversity and habitat values for each of the habitat types, for mammals only, as they are able to choose preferred habitats. In order to evaluate biodiversity, we used Maxent to derive species richness map and used InVEST's Habitat quality model to evaluate habitat value. As a result of evaluation, species richness was high in the national park boundary area. Also, even if the same edge is adjacent to the development area depending on the land cover, the species richness is low. Compared with Wolaksan and Sobaeksan National Park, the species richness and habitat value of the northern area, which is connected with other forests, were higher than those of the southern area where roads were developed. Therefore, it is expected that the use of the result of this study for the national park boundary adjustment and management will enhance the function of the national park as a habitat.