신경 향상 기술 관련 신경윤리 연구를 위한 시나리오 개발 -뇌심부자극술(DBS)을 통한 기억 향상 기술을 중심으로-

류영준,엄주희 동국대학교 동서사상연구소 2022 철학·사상·문화 Vol.- No.39

This study contains the process of deriving specific technical ethics scenarios for neuroethics research. It based on the results of a human trial study of deep brain stimulation for the purpose of improving memory, which was recently conducted at Seoul National University Hospital and known through the media. This scenarios process was based on the results at Seoul National University hospital in 2020, presented by Professor Chun Kee Chung, a neurosurgeon and Soyeon Jeon a neuroscientist at the Department of Brain and Cognitive Science at Seoul National University. Based on this study, the scenario derivation method was divided into two stages. First of all, it was checked whether the basis for the preparation of the technical ethics scenario was appropriate in the six criteria. Criteria are 1) researcher's credibility and the existence of scientific basis, 2) unethical or illegality of research procedures, 3) commercial research purpose, 4) scientific specificity, 5) potential for development 6) Possibility of social impact. The “Deep Brain Stimulation Technical Ethics Scenario for the purpose of improving memory for test takers” thus derived is expected to be a necessary basis for neuroethicists and humanities scholars who wish to discuss humanities.

高秉喆, 金世珍, 朴在圭, 李永鎬, 崔昌澗 共著, 北韓外交論, (서울 : 慶南大學校 極東問題硏究所 硏究씨리즈 9, 1977)

劉英俊 한국국제정치학회 1977 國際政治論叢 Vol.17 No.-

Phenotypic Characterization and ln Vivo Localization of Human Adipose-Derived Mesenchymal Stem Cells

류영준,조재진,조태준,이동섭,최진영 한국분자세포생물학회 2013 Molecules and cells Vol.35 No.6

Human adipose-derived mesenchymal stem cells (hADMSCs) are a potential cell source for autologous cell therapy due to their regenerative ability. However, detailed cytological or phenotypic characteristics of these cells are still unclear. Therefore, we determined and compared cell size, mor-phology, ultrastructure, and immunohistochemical (IHC) expression profiles of isolated hADMSCs and cells located in human adipose tissues. We also characterized the localization of these cells in vivo. Light microscopy examination at low power revealed that hADMSCs acquired a spindle-shaped morphology after four pas-sages. Additionally, high power views showed that these cells had various sizes, nuclear contours, and cytoplasmic textures. To further evaluate cell morphology, transmission electron microscopy was performed. hADMSCs typically had ultrastructural characteristics similar to those of primitive mesenchymal cells including a relatively high nuclear/cytosol ratio, prominent nucleoli, immature cytoplasmic organelles, and numerous filipodia. Some cells contained various numbers of lamellar bodies and lipid droplets. IHC staining demonstrated that PDGFR and CD10 were constitutively expressed in most hADMSCs regardless of passage num-ber but expression levels of -SMA, CD68, Oct4 and c-kit varied. IHC staining of adipose tissue showed that cells with immunophenotypic characteristics identical to those of hADMSCs were located mainly in the peri-vascular ad-ventitia not in smooth muscle area. In sum-mary, hADMSCs were found to represent a heteroge-neous cell population with primitive mesenchymal cells that were mainly found in the perivascular adventitia. Furthermore, the cell surface markers would be CD10/PDGFR. To obtain defined cell populations for therapeutic purposes, further studies will be required to establish more specific isolation methods.

한국의 연구중심병원의 연구용 인체조직 관리체계 향상을 위한 제언

류영준,김한겸,장세진 대한의사협회 2012 대한의사협회지 Vol.55 No.3

In this paper, the authors suggest more effective conditions for human tissue preparation in research hospitals. Because recent genetic and molecular studies have contributed to the rapid development of molecular and genetic medicine, human tissue is now again being considered as a valuable research resource. Basically, high-quality research-oriented tissue bank organizations are a very important part of a research hospital. The current management system for human tissue, however, is not very effective from either legal or practical perspectives. In this article, the authors propose some improvements on the human tissue management system. The laws on human tissue such as the Bioethics and Safety Act should be changed to contain suitable language applying on-site and efficient multi-dimensional information. Informed consent should be an essential requirement before surgery. Pathologists should be supported as the essential manpower of human tissue banks by law. A committee composed of a clinician, researcher, pathologist, information manager, and coordinator should be established to manage human tissue banks in hospitals. The Institutional Review Board should pay more attention to preventing the leakage of the private information of donors, and researchers should know about the review process and guidelines. This suggestion will create a more stable and effective system for the management of human tissue banks, and it will also create a complete and integrated system for research institutions. Therefore, human tissue banks can play an important role in improving research hospitals’ competitiveness by providing a valuable collection of material for research.

Embryonal Rhabdomyosarcoma Arising from a Mediastinal Teratoma: An Unusual Case Report

류영준,유수현,정민정,장세진,조경자 대한의학회 2013 Journal of Korean medical science Vol.28 No.3

We report an unusual case of 9.5-cm-sized embryonal rhabdomyosarcoma arose from a mediastinal mature teratoma in a 46-yr-old man. A man presented with chest trauma as a result of an accident at 10 September 2011. On chest X-ray, an anterior mediastinal mass was detected. To obtain further information, chest computed tomography (CT) with contrast enhancement was performed, revealing an anterior mediastinal mass. Complete surgical excision was performed and entire specimen was evaluated. Pathologic diagnosis was embryonal rhabdomyosarcoma arising in mature cystic teratoma. After surgical excision, two cycles of dactinomycin-based chemotherapy were performed. Lung metastasis was detected on follow up CT in September 2012, and wedge resection was performed. Pathological finding of the lung lesion showed same feature with that of primary rhabdomyosarcoma.

Analysis of Human Tissue Management Models for Medical Research: Preparation for Implementation of the 2012 Revision of the Bioethics and Safety Act of Korea

류영준,김한겸,장세진,구영모 대한의학회 2013 Journal of Korean medical science Vol.28 No.6

Efficient management of human tissue samples is a critical issue; the supply of samples is unable to satisfy the current demands for research. Lack of informed consent is also an ethical problem. One of the goals of the 2012 revision of Korea’s Bioethics and Safety Act was to implement regulations that govern the management of human tissue samples. To remain competitive, medical institutions must prepare for these future changes. In this report, we review two tissue management models that are currently in use; model 1 is the most common system utilized by hospitals in Korea and model 2 is implemented by some of the larger institutions. We also propose three alternative models that offer advantages over the systems currently in use. Model 3 is a multi-bank model that protects the independence of physicians and pathologists. Model 4 utilizes a comprehensive single bioresource bank; although in this case, the pathologists gain control of the samples,which may make it difficult to implement. Model 5, which employs a bioresource utilization steering committee (BUSC), is viable to implement and still maintains the advantages of Model 4. To comply with the upcoming law, we suggest that physicians and pathologists in an institution should collaborate to choose one of the improved models of tissue management system that best fits for their situation.

韓國歷代政權의 國家目標設定과 그 政治的 課題

劉英俊 한국정치학회 1980 한국정치학회보 Vol.14 No.-

Nylon6 Non-Woven with Nano Fiber

류영준,강영식,김학용,이덕래 한국고분자학회 2001 한국고분자학회 학술대회 연구논문 초록집 Vol.2001 No.10

랑게르한스세포 조직구증 20예의 병리학적 특징과 Langerin (CD207)의 면역화학염색의 발현 특이성

류영준,정회선,신봉경,김애리,김한겸,김인선,이현주,이상호 대한병리학회 2009 Journal of Pathology and Translational Medicine Vol.43 No.2

Background : Langerhans cell histiocytosis (LCH) is a well-known neoplastic disorder of Langerhans cells which has characteristic findings, however, LCH has not been adequately studied in Korea. Methods : We analyzed the clinicopathologic features of 20 patients with LCH who were diagnosed between 1997 and 2006 at the Korea University Guro and Anam Hospitals. Results : The M:F ratio was 3:1 and the age ranged from 2-60 years (mean, 23.8 years [4 in 1st decade, 6 in 2nd decade, 2 in 3rd decade, 5 in 4th decade and 3≥40 years of age). The cases were classified as unifocal unisystemic in 13 patients, multifocal unisystemic in 4 patients, and multifocal multisystemic in 3 patients. The bone was the most commonly involved organ (14), followed by lymph node (5), lung (2), skin (2) and ureter (1). The Langerhans cells were immunohistochemically stained with Langerin, CD1a, S-100 protein, and CD68. Langerin and CD1a were specific for Langerhans cells. Conclusions : The distribution of the involved organs in patients with LCH was similar to the distribution in Western countries,but lymph node involvement was more frequent, whereas lung involvement was less common. Langerin is considered to be a specific marker for Langerhans cells. Background : Langerhans cell histiocytosis (LCH) is a well-known neoplastic disorder of Langerhans cells which has characteristic findings, however, LCH has not been adequately studied in Korea. Methods : We analyzed the clinicopathologic features of 20 patients with LCH who were diagnosed between 1997 and 2006 at the Korea University Guro and Anam Hospitals. Results : The M:F ratio was 3:1 and the age ranged from 2-60 years (mean, 23.8 years [4 in 1st decade, 6 in 2nd decade, 2 in 3rd decade, 5 in 4th decade and 3≥40 years of age). The cases were classified as unifocal unisystemic in 13 patients, multifocal unisystemic in 4 patients, and multifocal multisystemic in 3 patients. The bone was the most commonly involved organ (14), followed by lymph node (5), lung (2), skin (2) and ureter (1). The Langerhans cells were immunohistochemically stained with Langerin, CD1a, S-100 protein, and CD68. Langerin and CD1a were specific for Langerhans cells. Conclusions : The distribution of the involved organs in patients with LCH was similar to the distribution in Western countries,but lymph node involvement was more frequent, whereas lung involvement was less common. Langerin is considered to be a specific marker for Langerhans cells.

트립티센 기반의 트리페닐아민 전자-주게 분자 합성 및 특성 분석

류영준,안병관 한국전기전자재료학회 2022 전기전자재료학회논문지 Vol.35 No.4

The development of efficient electron donor (or hole-transporting) molecules that can be used in variousoptoelectronic device fields is highly demanded. In this work, a novel class of triptycene-based three-dimensional (3D)triphenylamine (TI-TPA) derivatives with different end substituents was designed and prepared for transparent electron donor materials. Owing to the rigid 3D triptycene framework, the obtained TI-TPA derivatives had an amorphous morphology with high thermal decomposition temperature. The oxidation potential of these TI-TPA derivatives decreased as the electron donating strength of the end substituent increased. Among TI-TPA derivatives, TI-TPA-OMe exhibited the highest HOMO level (-5.31 eV) which is similar to that of Spiro-OMeTAD (-5.22 eV). In addition, TI-TPA-OMe was found to form a strong charge transfer complex with the triptycene-based acceptor TI-BQ, leading to a new absorption band at around 640 nm. These results can be applied for developing efficient electron donor materials that can mimic the advantages of the spiro-linked structure and TPA units of Spiro-OMeTAD.