Betulin suppressed interleukin-1b-induced gene expression, secretion and proteolytic activity of matrix metalloproteinase in cultured articular chondrocytes and production of matrix metalloproteinase in the knee joint of rat

라호종,이충재,황선철,이현재,조호성,남대철,이영복,강병훈,문동규,김동희 대한약리학회 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.1

We investigated whether betulin affects the gene expression, secretion and proteolytic activity of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as in vivo production of MMP-3 in the rat knee joint to evaluate the potential chondroprotective effect of betulin. Rabbit articular chondrocytes were cultured and reverse transcription-polymerase chain reaction (RT-PCR) was used to measure interleukin-1b (IL-1b)-induced gene expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), ADAMTS-5 and type II collagen. Effect of betulin on IL- 1b-induced secretion and proteolytic activity of MMP-3 was investigated using western blot analysis and casein zymography, respectively. Effect of betulin on MMP-3 protein production was also examined in vivo. The results were as follows: (1) betulin inhibited the gene expression of MMP-3, MMP-1, MMP-13, ADAMTS-4, and ADAMTS-5, but increased the gene expression of type II collagen; (2) betulin inhibited the secretion and proteolytic activity of MMP-3; (3) betulin suppressed the production of MMP-3 protein in vivo. These results suggest that betulin can regulate the gene expression, secretion, and proteolytic activity of MMP-3, by directly acting on articular chondrocytes.

운동선수에서 횡방향 나사 고정 후 발생한 슬개골의 골절 - 증례 보고 -

라호종,김성태,하정구,김진구,Ra, Ho Jong,Kim, Sung Tae,Ha, Jeong Ku,Kim, Jin Goo 대한정형외과스포츠의학회 2011 대한정형외과스포츠의학회지 Vol.10 No.2

전위성 슬개골 골절에 있어서 해부학적 정복 및 견고한 고정은 슬관절 기능과 강도, 그리고 조기 관절 운동 범위의 회복을 위해서 필요하다. 골절의 형태와 다양한 수술 기법에 따라서, 수술 후 많은 합병증들이 보고 되어져 왔다. 본 저자들은 운동 선수들에게서 발생한 종골절 1예와 이분성 슬개골의 골편 전위 골절 1예에서 유관 나사못 횡 고정술을 시행한 뒤 새로운 횡골절이 발생한 증례를 경험하여 보고하고자 한다. Anatomical reduction and strong fixation in displaced patella fracture are needed for restoration of knee function and strength and early range of motion exercise. According to the type of fracture and various operational methods, their many complications have been reported. We report 2 cases of transverse patella fracture which were caused by transverse screw fixation in longitudinal patella fracture and fracture of bipartite patella in athletes.

다발성 슬관절 인대 손상과 동반된 총 비골 신경 손상

라호종,이기원,조형권,호병철,서동교,최영준 대한스포츠의학회 2018 대한스포츠의학회지 Vol.36 No.1

Common peroneal nerve (CPN) injury associated with multiple-ligament knee injury is relatively rare. A 38-year-old male presented with left knee pain occurred during ssireum (Korean wrestling). The patient exhibited positive Lachman, grade 3 varus stress, and also positive dial testing at 30o. Sensory loss of some area of left foot and foot drop were observed. Magnetic resonance imaging showed complete anterior cruciate ligament (ACL) rupture and posterolateral corner (PLC) injury. Motor nerve conduction velocity indicated left CPN palsy. ACL and PLC reconstructions were performed 10 days after injury and nerve exploration was done simultaneously. Neurolysis and primary repair were also performed. At 26 months after injury, muscle power of the tibialis anterior and extensor hallucis longus improved to grade 3, and sensation in CPN area recovered to about 60%. There was good stability in injured knee. To the best of our knowledge, this is the first case report describing CPN palsy with multiple-ligament knee injury during ssireum.

Effects of salmon DNA fraction in vitro and in a monosodium iodoacetate-induced osteoarthritis rat model

라호종,오미영,김희주,이승용,엄대운,이석규,김수남,정규성,장혁재 대한약리학회 2018 The Korean Journal of Physiology & Pharmacology Vol.22 No.2

PRF001 is a fragmented DNA polymer extracted from the testes of salmon. The purpose of this study was to assess the anti-inflammatory effect of PRF001 in vitro as well as the protective effect of PRF001 intake against arthritis in a rat model. In vitro, cell survival and inflammatory markers after H2O2 treatment to induce cell damage were investigated in CHON-001 cells treated with different concentrations of PRF001. In vivo, osteoarthritis was induced by intra-articular injection of monosodium iodoacetate (MIA) into the knee joints of rats. After consumption of PRF001 (10, 50, or 100 mg/kg) for 4 weeks, inflammatory mediators and cytokines in articular cartilage were investigated. In vitro, the levels of inflammatory markers, IL-1β, TNF-α, COX-2, iNOS, and PGE2, were significantly suppressed by PRF001 treatment. In vivo, the inflammatory mediators and cytokines, IL-1β, p- Erk1/2, NF-kB, TNF-α, COX-2, and PGE2, as well as MMP3 and MMP7, which have catabolic activity in chondrocytes, were decreased in the MIA-induced osteoarthritic rats following intake of PRF001. Histological analysis revealed that PRF001 had a protective effect on the articular cartilage. Altogether, these results demonstrated that the anti-inflammatory property of PRF001 contributes to its protective effects in osteoarthritis through deregulating IL- 1β, TNF-α, and subsequent signals, such as p-Erk1/2, NF-κB, COX-2, PGE2, and MMPs.

일단계 전방 십자 인대 재 재건술

라호종,하정구,김상범,성정환,서정국,김진구,Ra, Ho-Jong,Ha, Jeong-Ku,Kim, Sang-Bum,Sung, Jung-Hwan,Seo, Jeong-Gook,Kim, Jin-Goo 대한관절경학회 2009 대한관절경학회지 Vol.13 No.2

목적: 본 논문의 목적은 전방 십자 인대 재 재건술의 원인을 살펴보고, 일단계 재 재건술의 수술 술기의 유용성을 알아보고자 하는데 있다. 대상 및 방법: 2004년 11월부터 2008년 7월까지 전방 십자 인대 재 재건술을 시행한 33명을 대상으로 하였다. 전방 십자 인대 재 재건술의 원인은 대퇴터널의 종적 배열이 22예, 후외방 불안정성의 간과가 7예, 재건술 후 심한 외상이 3예, 재건술 후 심부 감염이 1예였다. 재 재건술시 대퇴 터널의 위치는 10시 또는 2시 방향의 외측 방향으로 하고, 경골터널은 대부분 기존의 터널을 이용하였다. 이전 수술 때 만들어진 이상 위치의 대퇴 터널은 고정 나사못 등을 제거하고, 수술 시 동종골을 나사 형태로 만들어 골이식을 시행하였다. 결과: 추시 기간은 평균 22.2개월(12~52개월)이었으며, 추시 결과 Lysholm score, IKDC 점수는 각각 재 재건술 전 평균 $61.5{\pm}16.8$, $63.9{\pm}15.1$에서 재수술 후 $86.3{\pm}11.5$, $81.3{\pm}14.3$으로 향상되었으며, KT-2000 arthrometer는 평균 $6.0{\pm}2.2\;mm$에서 $1.6{\pm}1.4\;mm$로 줄어들었다. 결론: 전방 십자 인대 일단계 재 재건술은 양호한 고정과 임상 결과로 유용한 술식으로 사료된다. Purpose: To investigate the causes of failure on ACL reconstructions and evaluate the effectiveness of one stage revision ACL reconstructions. Materials and Methods: From November 2004 to July 2008, thirty three patients who had got revision ACL recontstructions after reruptures of ACL were evaluated. The causes of failure of ACL reruptures were 22 vertical femoral tunnels, 7 neglected PLRI, 3 severe traumas and 1 deep infection after ACL reconstruction. The femoral tunnels were aimed at the 10 or 2 o'clock position and the tibial tunnels were used with previous tunnels. Previous femoral screws from the improper femoral tunnels were removed and filled with the new allograft bones. Results: The average periods of follow up were 22.2 months (12~52 months). There was improvement on an average Lysholm knee score from $61.5{\pm}16.8$ to $86.3{\pm}11.5$, IKDC score from $63.9{\pm}15.1$ to $81.3{\pm}14.3$. Mean side to side difference was decreased from $6.0{\pm}2.2\;mm$ to $1.6{\pm}1.4\;mm$ using KT-2000 arthrometer. Conclusion: One stage revision ACL reconstruction can be a useful method with good clinical results.

제 5형 SLAP 병변에 대하여 Bio-knotless 봉합 나사못을 이용한 관절경적 봉합 수술의 임상적 결과

염재광,이상림,라호종,Yum, Jae-Kwang,Lee, Sang-Lim,Ra, Ho-Jong 대한관절경학회 2007 대한관절경학회지 Vol.11 No.1

목적: 견관절의 제 5형 SLAP 병변에 대하여 bio-knotless 봉합 나사못을 이용하여 관절경적 봉합을 시행한 후 추시한 결과 만족할 만한 임상적 결과를 얻었기에 이를 보고하고자 한다. 대상 및 방법: 지속적인 추시가 가능하였던 10례에 대하여 임상적 결과를 분석하였으며 평균 연령은 32.7세($20{\sim}49$)였고 모두 남자였다. 손상의 원인은 7례에서 스포츠 손상이었으며 1례가 산업 재해, 2례가 교통 사고였다. 처음 수상한 날로부터 수술까지의 기간은 평균 47.2개월이었으며 수술 전 Rowe 점수는 평균 37.5점($30{\sim}55$)이었다. 모든 예에서 SLAP 병변을 먼저 관절경적으로 1개 또는 2개의 bio-knotless 나사못을 사용하여 봉합한 다음 Bankart 병변을 봉합하였는데 3개의 bio-knotless 나사못을 삽입한 경우가 7례, 2개를 삽입한 경우가 3례였다. 수술 후 3주간 상지 외전 보조기를 착용시키고 매일 간헐적인 수동적 견관절 굴곡-외회전-내회전 운동을 시행하였고, 관절의 운동 범위가 정상이 되면 회전근 개의 근력 회복을 위한 운동 치료를 시행하였고, 마지막 단계에서는 삼각근 및 견갑골 주위 근육 강화를 위한 근력 강화 운동을 시행하였다. 결과: 평균 추시 기간은 15.7개월($6{\sim}27$)이었으며 최종 추시시 견관절의 운동 범위는 8례에서 완전한 회복을 보였으나 산업 재해에 의한 손상의 환자에서는 굴곡 170도, 상지 내전 상태에서의 외회전 60도, 내회전은 제 12 흉추 범위를 보였으며 교통사고로 수상한 1례에서 견관절의 능동적 운동 범위가 굴곡 160도, 상지 내전 상태에서의 외회전 45도, 내회전은 제 12 흉추 범위를 보였으나 일상 생활에는 문제가 없었다. 최종 추시시 Rowe 점수는 평균 93점($75{\sim}100$)으로 모두 우수한 결과의 소견을 보였다. 결론: 제 5형 SLAP 병변에 대하여 bio-knotless를 이용한 관절경적 봉합 수술이 만족할 만한 임상적 결과의 소견을 보여 매우 좋은 수술 방법으로 사료된다. Purpose: This study reports the clinical results of the arthroscopic repair of type V SLAP lesion with bio-knotless anchor. Materials and Methods: 10 cases of 10 patients (10 male) were included in this study. The average age was 32.7 years old and the period from the first injury to operation was average 47.2 months. Preoperative Rowe score was average 37.5. Arthroscopic SLAP repair with 1 or 2 bio-knotless anchors were performed and arthroscopic Bankart repair with bio-knotless anchors were performed in all cases; 3 anchors were used in 7 cases and 2 anchors in 3 cases. The average follow up period was 15.7 months. Results: The Rowe score improved to 93 at last follow up period and 8 cases had full range of motion of the shoulder. 2 case had mild limited range of motion of the shoulder (one case; 170 degrees in flexion, 60 degrees in external rotation and T12 level in internal rotation, the other case; 160 degrees in flexion, 45 degrees in external rotation and T12 level in internal rotation.) without any problem in normal activity. Conclusion: Arthroscopic repair with bio-knotless anchor in type V SLAP lesion is one of the good methods because of the good clinical results.

Perioperative Risk Assessment in Patients Aged 75 Years or Older: Comparison between Bilateral and Unilateral Total Knee Arthroplasty

최영준,이현일,라호종,황도연,김태경,심상준 대한슬관절학회 2014 대한슬관절학회지 Vol.26 No.4

purpose: The purpose of this study is to evaluate the risk of sequential bilateral total knee arthroplasty (TKA) under 1 anesthesia in patients 75 years or older.materials and methods: Patients aged 75 years or older who underwent sequential bilateral TKA (bilateral group, n=159) and unilateral TKA (unilateral group, n=159) between 2002 and 2012 were selected. All patients were evaluated for underlying medical diseases, such as cardiac, pulmonary, and renal problems, and high-risk patients were recommended to postpone the surgery. We compared the underlying diseases, major postoperative complications, and the length of hospital stay between bilateral and unilateral groups.results: The prevalence of underlying diseases of the bilateral group was 74.8% and major complications occurred in 6 patients (3.8%). The prevalence of underlying diseases of the unilateral group was 52.4% and complications were observed in 4 patients (2.4%). Although the complication rate of the bilateral group was slightly higher than that of the unilateral group, the difference was not statistically meaningful (p=0.204). The length of hospital stay was 21.9 days for the bilateral group and 24.9 days for the unilateral group.conclusions: There was no significant difference in postoperative complications between groups. The result shows that bilateral TKA can be relatively safe compared with unilateral TKA in patients 75 years or older. However, careful selection of low-risk patients is advised.

회전근개 봉합시 용해성 실을 이용한 변형 Mason-Allen 봉합술식

이상진,박현철,조근호,라호종,김관수 대한견주관절의학회 2011 대한견주관절학회 학술대회논문집 Vol.2011 No.3

Arthroscopic Transfossa Approach

이상진,박현철,조근호,라호종,김관수 대한견주관절의학회 2011 대한견주관절학회 학술대회논문집 Vol.2011 No.3

외측 반월상 연골의 방사형 완전 파열 시 섬유성 응괴를 이용한 봉합술

하정구(Jeong Ku Ha),김진구(Jin Goo Kim),라호종(Ho Jong Ra) 대한정형외과학회 2010 대한정형외과학회지 Vol.45 No.1

흔하게 발생하는 슬관절 손상 중의 하나인 반월상 연골 파열은 기능적 중요성이 강조됨에 따라 점차 기능을 보존하는 방향으로 다양한 치료방법이 연구되고 있다. 방사형 완전 파열은 반월상 연골의 원주 테 장력을 소실시키기 때문에 봉합하여 기능을 보존하는 것이 중요하지만 손상부위의 구조적 특성상 치유가 쉽지 않은 것으로 알려져 있다. 저자들은 외측 반월상 연골의 방사형 완전 파열 손상을 입은 5예의 환자에 대해 섬유성 응괴를 이용한 봉합술을 시행하여 전례에서 양호한 반월상 연골의 치유와 함께 좋은 임상 결과를 얻었기에 보고하는 바이다. Meniscus tear is one of the most common knee injuries. The increasing recognition of the importance of the meniscal function has led to a range of new treatments for meniscal tears. A complete radial tear disrupts the circumferential hoop tension of the meniscus. Therefore, restoring its natural architecture is of utmost importance. However, it is difficult to achieve meniscal healing of a complete radial tear due to its structural characteristics. We report five cases of a complete radial tear of the lateral meniscus treated successfully using a fibrin clot that showed good meniscal healing and excellent clinical outcomes.