理中湯과 理中湯加味方의 抗알레르기 效果에 관한 實驗的 硏究

남혜정,채병윤,Nam, Hae-Jeong,Chae, Byung-yoon 대한한방안이비인후피부과학회 2000 한방안이비인후피부과학회지 Vol.13 No.1

Experimental studies were done to research effects of Leejungtang and Leejungtanggamibang on the anti-allergic effects. We studied the vacular permeability response induced by histamine and serotonin, homologous PCA provoked by IgE-like antibody against EWA, contact dermatitis induced by picryl chloride and delayed type hypersensitivity response to SRBC. The results were obtained as follows: 1. In effects of the Leejungtang and Leejungtanggamibang on the vascular permeability responses to intradermal serotonin, the Leejungtang revealed insignificant effect and Leejungtanggamibang revealed significantly inhibitory effect. 2, In effects of the Leejungtang and Leejungtanggamibang on the vascular permeability responses to intradermal histamine, the Leejungtang revealed insignificant effect and Leejungtanggamibang revealed significantly inhibitory effect. 3. In the homologous PCA provoked by IgE-like antibody against white albumin, the Leejungtang revealed insignificant effect and Leejungtanggamibang revealed significantly inhibitory effect. 4. In the delayed type hypersensitivity responses to picryl chloride, the Leejungtang revealed insignificant effect and Leejungtanggamibang revealed significantly inhibitory effect. 5. In the delayed type hypersensitivity responses to sheep red blood cell the Leejungtang revealed insignificant effect and Leejungtanggamibang revealed significantly inhibitory effect.

노화와 피부노화에 대한 고찰

남혜정,김윤범,Nam Hae-jeong,Kim Yoon-bum 대한한의진단학회 2004 大韓韓醫診斷學會誌 Vol.17 No.1

In Oriental medicine, aging is just a natural process like change of seasons. Ancient Oriental people accepted it as a natural thing to be growing older and to die at last. The science of aging has advanced dramatically. In the last 2 decades, advances in genetics and molecular biology have led to extraordinary new understandings in how cells age, how apoptosis programs cells to die, and how neuroendocrinology plays a role in the lifespan of organisms. Today, the matter of primary concern about aging is a cellular and mitochondrial damage of human body induced by reactive oxygen species(ROS). The skin aging can be divided into two areas, intrinsic(chronologic)-aging and photo-aging. There are lots of photo damage about skin aging. The skin is increasingly exposed to ultraviolet(UV) irradiation in life. Therefore, the risk of photo-oxidative damage of the skin induced by reactive oxygen species(ROS) has increased substantially. Nowadays, many people believe that they can stop or at least delay the process of aging. There are lots of treatments that promise to slow the process of aging and the associated ailments. Many of these treatments, for example, exercise, Vit E, Vit C therapy, hormone therapy, restrict diet, are gradually being subjected to clinical trials. But in spite of all efforts, researches and investigations, there is no single method or treatment which is revealed to be truly effective for delaying progress of aging. Every methods insisted on effect for delaying aging process, has its dark side. All we can do is just keeping ourself healthy until the time of death.

과루인(瓜蔞仁)이 장기간 고용량 corticosteroid 투여 hairless mouse의 피부장벽에 미치는 영향

남혜정,김규석,김윤범,Nam, Hae-Jeong,Kim, Kyu-Seok,Kim, Yoon-Bum 대한한방안이비인후피부과학회 2008 한방안이비인후피부과학회지 Vol.21 No.3

Objective : Prolonged use of systemic corticosteroids negatively impacts skin barrier function. Corticosteroids have a major role in the practical management of many diseases, so it is necessary to find the drug or supplement which could keep the skin healthy during the systemic corticosteroids therapy. Seed of Trichossnthcs kiiilowii was commonly used for pulmonary disease in traditional Chinese medicine. Many studies have investigated and found that seed of Trichosanthes kirilowii has anti-bacterial, anti-HIV, anti-tumor and immunoregulatory functions. This study was performed to investigate the effect of seed of Trichosmthes kirilowii on the Skin Barrier Method : Triamcinolone 0.4mg was injected male hairless mice for 5 weeks ( 2 times a week, totally 10 times), Just before the first injection, hairless mice were divided into 3 groups; Control ( normal saline medicated group ), GroupA( seed of Trichosuuhes kirilowii qd/day medicated group ) and GroupB( seed of Trichosuuhes kiiilowii bid/day medicated group ), Body weight of all the groups were checked during the experiment, After $10^{th}$ injection, TEWL (Transeidermal water loss) of 3 groups were artificially increased by 9 times tape stripping and the changes of TEWL were checked at before stripping, right after, 2h, 4h, 6h, 24h, 48 and 72h later after stripping, AST, ALT and CBC were also checked. Indeed, stratum corneum of 3 groups were also examined and compared with that of normal hairless mouse. Results : 1. GroupB showed significantly lower body weight among three groups 2, There was no statistical difference at AST, ALT and CBC among three groups 3. GroupB was showed significantly lower TEWL than TEWL of Control at 48h later. 4 GroupB recovered it's TEWL of before tape stripping at 24h later, GroupA recovered that at 48h and Control recovered that at 72h later at the same condition 5. Stratum corneum of GroupA and GroupB were looked almost normal and healthy. On the contrary, stratum corneum of Control was looked thin and unhealthy. Conclusions : Seed of Ttichossnthes kirilowii has some effects on skin barrier function, especially TEWL of high dose corticosteroids injected hairless mouse and the efficacy seemed to be related with the dosage.

태반이 피부장벽에 미치는 영향

남혜정,박외숙,김희정,김규석,차재훈,김윤범,Nam, Hae-Jung,Park, Owe-Suk,Kim, Hee-Jeong,Kim, Keoo-Seok,Cha, Jae-Hoon,Kim, Yoon-Bum 대한한방안이비인후피부과학회 2006 한방안이비인후피부과학회지 Vol.19 No.2

Objectives : This experimental study was performed to investigate the effects of the Hominis placenta extracts on skin barrier. Methods : Male hairless mice, average weight 20g, were divided into two groups, intact and treatment group(paired, n=15). Intact group was not applied YB-301(an ointment including Hominis placenta). Treatment group was applied YB-301(an ointment including Hominis placenta) two times a day for 8 days. We observed skin melanin, skin erythema, skin pH, skin humidity, transepidermal water loss. Statistical analysis was performed by using paired sample T-test. Statistical significance was achieved if the probability was less than 5%(p<0.05) or 1%(p <0.01) Results : 1. YB-301(an ointment including Hominis placenta) showed statistically significant effect on skin melamin, skin pH, skin humidity(p<0.05). 2. YB-301(an ointment including Hominis placenta) showed statistically significant inhibitory effect on transepidermal water loss(p<0.01). 3. YB-301(an ointment including Hominis placenta) showed statistically no significant effect on skin erythema(p<0.05). conclusions : YB-301(an ointment including Hominis placenta) was effective m skin melanin, skin pH, skin humidity, transepidermal water loss in our study, so we suggest that Hominis placenta can be used as a ointment ingredient for strengthening the function of skin barrier.

인참양영탕(人參養榮湯)이 장기간 고용량 스테로이드 투여 hairless mice의 피부장벽에 미치는 영향

남혜정,김윤범,Nam, Hae-Jeong,Kim, Yoon-Bum 대한한방안이비인후피부과학회 2008 한방안이비인후피부과학회지 Vol.21 No.2

Objective : The side effect of prolonged use of topical corticosteroids to skin is well-known. Moreover, resent studies have shown that prolonged use of systemic corticosteroids also negatively impacts skin barrier function. Corticosteroids have a major role in the practical management of many variable conditions. So, it is important to find the drug or the method which could protect the skin from the damage caused by corticosteroids. At former study, we investigated that Insamyangyoung-tang has some effect on skin barrier function of DNCB induced contact dermatitis hairless mice. So, this study was performed to research the effect of Insamyangyoung-tang on the TEWL with high dose steroid injected hairless mice. Method : Hairless mice were divided into 4 groups ; Control group, Group A, Group B and Group C. All groups were injected triamcinolone O.4mg for 10 times. Control group was medicated distilled water during the experimental period. Group A was medicated distilled water for 5 days before the $1^{st}$ injection day, and then medicated Insamyangyoung-tang extract during the experimental period. Group B was medicated Insamyangyoung-tang extract for 5 days before the $1^{st}$ injection day, and then medicated distilled water during the experimental period. Group C was medicated Insamyangyoung-tang extract for 5 days before the $1^{st}$ injection day, and then medicated Insamyangyoung-tang extract during the experimental period. TEWL of each group was measured for 5 times. After the $10^{th}$ injection, the tissue sample was made and the damage of epithelial cell was examined. Statistical significance was set at p<0.05 by using non parametric methods and repeated-anova.. Results : Group C showed significant effect on TEWL change of hairless mice evoked by triamcinolone injection. Group A and Group B also showed some effect, but there was no statistical significance.

頭痛이 主訴인 소아 부비동염 환자의 치험 1例

남혜정,김윤범,Nam, Hae-jeong,Kim, Yoon-bum 대한한방안이비인후피부과학회 2003 한방안이비인후피부과학회지 Vol.16 No.1

Paranasal sinusitis has many different kinds of symptoms ; rhinorrea, cough. post nasal drip. headache, etc. In some cases, patients, mostly adults, had no symptom except headache. We treated a 8-year-old child who only complained headache without any other symptoms related in nasal disease. Her symptoms ; headache, nausea, vomiting and general weakness, showed typical type of disease which were due to phlegm. So we diagnosed her headache as a " Headache due to phlegm". Interestingly, her PNS images were diagnosed as bilateral maxillary sinusitis. After one and a half month treatment with herb-med and Aroma oil, the patient got free from all symptoms and her PNS images showed no abnormality.

만형자(蔓荊子), 시호(柴胡)의 알레르기성 비염에 대한 효과

남혜정,박외숙,김희정,김규석,차재훈,김윤범,Nam, Hae-Jeong,Park, Owe-Suk,Kim, Hee-Jeong,Kim, Keoo-Seok,Cha, Jae-Hoon,Kim, Yoon-Bum 대한한방안이비인후피부과학회 2006 한방안이비인후피부과학회지 Vol.19 No.2

Background and Objectives : In oriental Medicine, there is a concept that herb works only in it's own specific site called 'Guikyung(歸經)'. If two herbs have same effect and different Guikyung, two herbs would work at different site respectively. so we want to prove that whether Guikyung has significance or not in experimentally. Methods : In order to investigate the effects of Victicis Fructus & Bupleuri Radix on the Allergic rhinitis, BALB/c mouse was used. Control and experimental group 1, 2 were induced allergic rhinitis. Experimental group 1 was administered Victicis Fructus for 28 days and group 2 was administerd Bupleuri Radix for same period. WBC, neutrophil, lymphocyte, monocyte, IL-4 and $IFN-{\gamma}$ were measured and histological examination was performed. The statistical significance was examined by Independent Samples T test. Results : 1. WBC, neuoophil, lymphocyte, monocyte, IL-4 and $IFN-{\gamma}$ showed no statistically significant differences in three groups. 2. Histological examination had no differences in three groups. Conclusion: Victicis Fructus & Bupleuri Radix have no effect on allergic rhinitis and the reason is estimated that Guikymg plays a important roll to determine the effect of herb from the viewpoint of oriental medicine.

酒차鼻의 韓方治驗

남혜정,Nam, Hae-Jeong 대한한방안이비인후피부과학회 2002 한방안이비인후피부과학회지 Vol.15 No.2

Persistent facial telangiectasia, erythema and flushing are the major cosmetic problems in patients with rosacea. However various therapeutic modalities for rosacea are not effective. Five patients who had a history of rosacea which poorly controlled by oral and topical medications, were given Herb-medication and Acupuncture treatment. The size of erythema and the number of papules had been gradually decreased and finally all the symptoms were gone except telangiectasia.

돌발성 난청의 한방 치료

남혜정(Hae-Jeong Nam) 대한한의학회 2009 대한한의학회지 Vol.30 No.4

이명과 악관절장애간의 상호관계 문헌연구

김봉현,남혜정,Kim, Bong-Hyun,Nam, Hae-Jeong 대한한방안이비인후피부과학회 2011 한방안이비인후피부과학회지 Vol.24 No.3

Objective : We investigated relationship between tinnitus and temporomandibular disorder(TMD) and tried to find approach possibility in oriental medicine. Method : We searched Pubmed, Scopus, The Cocharane library and additional sources for published and unpublished trials. Search word was "Tinnitus and TMD" Result : We investigated 18 studies. We divided these studies into three parts, epidemiological studies, mechanical studies and treatment studies. In many epidemiological studies. there was significant relationship between tinnitus and TMD. The mechanism was not clear but in recently several studies proposed neurological approach. TMD treatment showed significant improvement in tinnitus accompanied by TMD. Conclusion : In tinnitus accompanied by TMD, TMD treatment can be available to resolve or reduce tinnitus. Especially in oriental medicine acupuncture can be applied to treat this.