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Aspergillus oryzae에서 다양한 promoter를 이용한 cellulase 유전자의 최적 발현조건 결정
남재국,함영태,정대균 경희대학교 유전공학연구소 1996 遺傳工學論文集 Vol.8 No.-
The purposes of this study are to express recombinant heterogeneous cellulase gene and to establish its optimal expression condition in Aspergillus oryzae. pVT103-U and pAN52-3 were used as vectors for the expression of a foreign cellulase. pVT103-U had yeast alcohol dehydrogenase (ADH) gene promoter and pAN52-3 had Aspergillus niger glyceraldehyde-3-phospho dehydrogenase (gpdA) gene promoter. Clostridium thermocellum endoglucanase (celA) gene was amplified by PCR and 1.4 kb PCR product was obtained. The 1.4 kb PCR products were ligated into pVT103-U and pAN52-3, and recombinant plasmids. pVT-CT and pAN-CT1 were obtained, respectively. After transformation of A. oryzae with recombinant plasmid by a protoplast method, the CMCase assay was carried out for the detection of cellulase expression level. The cellulase activity of pVT-CT increased 2.2 folds and the cellulase activity of pAN-CT1 increased 4.6 folds in comparision with the cellulase activity of host cell.
Filamentous fungus Aspergillus oryzae에서의 고발현 cellulase 형질전환 균체개발
오유택,정대균,함영태,남재국 中央大學校 遺傳工學硏究所 1995 遺傳工學硏究論集 Vol.8 No.1
The isolation of mutant strains is a prerequisite for transformation of Aspergillus orzae by complementation of an auxotrophic mutation using either a heterologous or homologous biosynthetic gene as a selectable marker. The transforming plasmid pILJ16 is a pUC8 derivative which contains Aspergillus nidulans argB for complementation of Aspergillus argBmutations. The frequency of transformation was low, 10-50 transformants per ug of input plasmid, and most of them were 'abortive'. Integration of a linear DNA molecule into the chromosome by homologous recombination involves two recombination events rather than the single event required for integration of a circular DNA molcerule. Cotransformation is a potentially powerful tool since it does not depend on the availability of selectable markers on the plasmid of choice. The plasmids pILJ-16 and YEp-JOI(or pYGAP-TH) selecting for complementation of the argB-marker. These cotransformant named JOIAγ, JOIB1, JOIAα2 and THBα-2. The specific enzyme activity in Aspergillus oryzae cotransformants is about 3 times higher than Aspergillus oryzae YTH-1.
황상구,남재국,Hwang, Sang-Koo,Nam, Jae-Guk 대한자원환경지질학회 2007 자원환경지질 Vol.40 No.1
The Sitting Buddha Carved on Rockcliff (National treasure No. 201) in Bukjiri consists of porphyritic biotite granite, which was fractured by three joint sets of NE-SW, EW and NS directions. They produced a physical weathering that broke many parts of the Buddha and background. The chemical index of alteration is 59 to 61 from the major elements in the granite that was weathered into producing kaolin minerals from alteration of feldspars and biotite. With weathering degree, major element compositions increase in $SiO_2$ and MnO, whereas decrease in $TiO_2,\;{Fe_2O_3}^t,\;MgO,\;CaO\;and\;K_2O$. Change proporations of trace elements to $Al_2O_3$ increase in all transition elements, Rb and Y, whereas decrease in Li, Sr and Ba. REE pattern increases only in HREE. Particularly, a decrease in CaO, $K_2O$, Sr and Ba results in what they are effluxed to dissolve from feldspars by groundwater. The Buddha image has been deteriorated into joints, color changes, brown rusts, granular decay, microorganic smears by the such weathering causes as deformation, moisture, temperature variation and microorganic living. The moisture, which leaks along the joints in the granite, not only dissolve to decompose minerals but also grows many microorganism and is frozen over during winter. NE-SW and NS joint sets affect to seep in water during rainy days to deteriorate the image because they extend outward. 봉화 북지리 마애여래좌상(국보 201호)은 흑운모 화강암으로 구성되고 NE-SW EW 및 NS 방향의 3개 절리조가 규칙적으로 나타난다. 이들은 마애여래좌상의 여러 부분을 심각하게 파손하는 기계적 풍화를 일으켰다. 이 화강암의 풍화암에서 주원소의 화학조성을 이용한 풍화지수는 토양층에서 $59{\sim}61$로 사장석이 용해되고 흑운모가 변질되어 카올린 광물의 생성이 활발한 방향으로 풍화작용이 진행되었다. 풍화도가 커질수록 주원소의 $SiO_2$, MnO는 약간 증가하는 반면에 $TiO_2,\;{Fe_2O_3}^t,\;MgO,\;CaO,\;K_2O$는 감소하는 경향을 보인다. $Al_2O_3$에 대한 미량원소의 변화량을 보면 모든 전이원소와 Rb, Y는 풍화도가 커질수록 증가하며, 반면에 Li, Sr, Ba은 감소를 나타낸다. REE의 패턴은 LREE에서 거의 일정하게 나타나지만 HREE에서 풍화도가 커질수록 증가하는 경향을 보인다. 특히 CaO, $K_2O$, Sr과 Ba의 감소는 장석들이 지하수에 용해되면서 유출된 결과이다. 마애여래좌상에서 훼손은 풍화에 의해 3방향의 절리, 착색현상, 입상분해, 미생물서식 등으로 나타난다. 풍화의 윈인은 주로 변형작용, 수분, 기온변화, 생물서식 등이다. 이 중에 수분이 화강암 내의 절리를 따라 스며들어 광물을 용해하고 분해할 뿐만 아니라 식물을 서식케 하고 결빙을 일으킨다. 북동 및 남북 절리조는 외곽부에서 내부로 연결되기 때문에 이 절리들은 빗물이 쉽게 마애여래좌상 내부로 스며들게 하여 앞으로 문화재를 훼손하는데 큰 영향을 미칠 것으로 생각된다.
김영미,남재국,조문재,신경수,박지은,김진영,임희경 대한의학회 2009 Journal of Korean medical science Vol.24 No.6
Chronic granulomatous disease (CGD) is a rare hereditary disorder characterized by recurrent life-threatening bacterial and fungal infections. The underlying defect in CGD is an inability of phagocytes to produce reactive oxygen species as a result of defects in NADPH oxidase. Considering that CGD generally affects about 3-4 in 1,000,000 individuals, it is surprising that the prevalence of CGD on Jeju Island is 20.7 in 1,000,000 individuals. We performed genetic analysis on 12 patients from 10 unrelated families and found that all patients had an identical homozygous single- base substitution of C to T in exon 1 (c.7C>T) of the CYBA gene, which was expected to result in a nonsense mutation (p.Q3X). Because Jeju Island has long been a geologically isolated region, the high prevalence of CGD on Jeju Island is presumably associated with an identical mutation inherited from a common ancestor or proband.