부비동 내시경 수술의 합병증에 대한 분석

김훈,조중생,이건희,황명구,홍석민,차창일 대한이비인후과학회 2002 대한이비인후과학회지 두경부외과학 Vol.45 No.8

Background and Objectives:Endoscopic sinus surgery (ESS) has been used worldwide since the advent of ES by Me-serklinger and Wigand. There have been many reports concerning the results of ESS on the treatment of chronic paranasal sinusitis, but few reports about the intraoperative complications of ESS in Korea. In this paper, we analyzed the intraoperative complications of ESS in Kyung He University Hospital and discussed about early recognition and managements. Materials and Method:We reviewed retrospectively the intraoperative complications of ESS of 1025 patients who underwent the ESS at Kyung Hee University Hospital from January 1998 to January 2001. Dividing the intraoperative complications into major and minor, we describe the managements and results. Results: % . The major complications occurred in 0.78% , with cerebrospinal fluid (CSF) leakage (0.39%) marking the comonest complication. The ratio of minor complications was 12.49% , with the leading one being the periorbital ecchymosis (6.15%) . Conclusion:ESS managements through preoperative survey of anatomical defects and improvement of operative skills. (Korean J Otolaryngol 2002;45:777-80)

Experimental Study on the Effects of GamiSamgieum (SGMX) on Hyperlipidemia

김훈,안정조,조현경,유호룡,설인찬,김윤식 대한한의학회 2009 대한한의학회지 Vol.30 No.3

Objectives:This study was aimed to investigate the effects of GamiSamgieum (SGMX) on hyperlipidemia using an animal model. Additionally, the underlying mechanisms were investigated by determination of gene expressions related with lipid metabolism. Methods:Forty mice were selected for use in this experiment, and then divided into five groups; Naive, Induced, SGMX 100, SGMX 200, and Lovastatin group as positive control with 8 mice each, and their blood was collected for analysis of blood and serum parameters. Results:Administration of SGMX significantly inhibited the increase of liver weight and the macrovacuolar cytoplasmic alterations in histopathologic finding. SGMX administration significantly protected the liver from pathologic elevation of AST and ALT and from lipid peroxidation. SGMX administration significantly lowered total cholesterol levels and TG, and partially upregulated the gene expression of LCAT, CYP7A1 and LDL-R receptor. Conclusion:SGMX is suggested to possess hypolipidemic effect, and thus could be a potential candidate for herbal hypolipidemic via further studies. Objectives:This study was aimed to investigate the effects of GamiSamgieum (SGMX) on hyperlipidemia using an animal model. Additionally, the underlying mechanisms were investigated by determination of gene expressions related with lipid metabolism. Methods:Forty mice were selected for use in this experiment, and then divided into five groups; Naive, Induced, SGMX 100, SGMX 200, and Lovastatin group as positive control with 8 mice each, and their blood was collected for analysis of blood and serum parameters. Results:Administration of SGMX significantly inhibited the increase of liver weight and the macrovacuolar cytoplasmic alterations in histopathologic finding. SGMX administration significantly protected the liver from pathologic elevation of AST and ALT and from lipid peroxidation. SGMX administration significantly lowered total cholesterol levels and TG, and partially upregulated the gene expression of LCAT, CYP7A1 and LDL-R receptor. Conclusion:SGMX is suggested to possess hypolipidemic effect, and thus could be a potential candidate for herbal hypolipidemic via further studies.

교정시설 내에서의 성적소수자 권리구제방안 : -국가배상을 중심으로-

김영관, 김훈, 송영균, 조비래 전남대학교 법률행정연구소 공익인권법센터 2012 인권법평론 Vol.- No.8

아시아 인프라 수요 : 2020년까지 약 8조 달러 수요 글로벌 인프라 투자의 절반 이상

조윤택,김훈상 포스코경영연구소 2015 CHINDIA Plus Vol.106 No.-

알레르기성비염환자에 대한 Astemizole - D 의 효과

조중생,박창식,여승근,안회영,차창일,방석혁,이흥만,정학열,정덕희,박재율,손영광,백정환,전경명,장백암,임재선,장혁순,백석인,이봉재,이용식,문영일,김영기,이상철,홍기환,임순광,김훈,양훈식,나기상,전병권,나익상,박영민,송기범,김경래 대한천식알레르기학회 1992 추계학술대회 초록집 Vol.1992 No.-

Cloning of Isoamylase Gene of Pectobacterium carotovorum subsp. carotovorum LY34 and Identification of Essential Residues of Enzyme

조계만,김은주,레누카라디아마스,샤모허마드아스라풀,홍선주,김종옥,신기재,이영한,김훈,윤한대,Cho, Kye-Man,Kim, Eun-Ju,Math, Renukaradhya K.,Asraful Islam, Shah Md.,Hong, Sun-Joo,Kim, Jong-Ok,Shin, Ki-Jae,Lee, Young-Han,Kim, Hoon,Yun, Han-Dae Korean Society of Life Science 2007 생명과학회지 Vol.17 No.9

연부균인 Pectobacterium carotovorum subsp. carotovorum LY34로부터 이소아밀라제 유전자 (glgX)를 클로닝한 후 대장균 숙주에서 발현시켰다. 이 효소는 ${\alpha}-1$,6-글루코시드 결합을 가수분해하였으나 ${\alpha}-1$,4-글루코시드 결합은 가수분해 하지 못하였다. 유전자는 658개의 아미노산을 암호화하는 1,977개의 DNA 염기서열로 이루어져 있었고 이 유전자에 의해 암호화되는 아미노산 서열을 다른 아밀라제 효소들과 비교한 결과 이소아밀라제 유전자와 유사하였으며 4개의 보존 지역을 확인하였다. SDS-PAGE에 의해 확인된 단백질의 크기는 약 74 kDa 이었다. 효소 활성은 pH 7.0, $40^{\circ}C$에서 가장 높은 활성을 나타났으며 $Ca^{2+}$ 첨가로 활성이 증가되었다. 이 효소의 보존되어 있는 아미노산 중에 글루탐산 370번, 아스파르트산 335번 및 442번 잔기를 알라닌으로 치환시킨 결과 활성이 약해졌다. 이 결과로부터 이들 잔기들이 효소활성에 중요한 역할을 하는 것으로 추정된다. The gene encoding for isoamylase of the Pectobacterium carotovorum subsp. carotovorum (Pcc) LY34 was cloned and expressed into Escherichia coli $DH5{\alpha}$. Isoamylase catalyzes the hydrolysis of ${\alpha}-1,6-glycosidic$ linkages specifically in amylopectin, glycogen, and derived oligosaccharides, while the enzyme did not hydrolyze ${\alpha}-1,4-glycosidic$ linkages of amylose. The isoamylase gene (glgX) had an open reading frame of 1,977 bp encoding 658 amino acid residues with a calculated molecular weight of 74,188 Da. The molecular weight of the enzyme was also estimated to be 74 kDa by activity staining of a SDS-PA gel. The mature GlgX had a calculated pI of 4.91. Isoamylase from Pcc LY34 had 70% amino acid identity with isoamylase from Pectobacterium chrysanthemi and contained the four regions conserved among all amylolytic enzymes. The isoamylase was optimally active at pH 7.0 and $40^{\circ}C$. GlgX was $Ca^{2+}-dependent$. The changes of Asp-335, Glu-370, and Asp-442 into Ala, respectively, using site-directed mutagenesis techniques showed that three residues are essential to isolamyalse (GlgX) activity. The sequences around those residues were highly conserved in isoamylase of different origins and GlgX of the glg operon in glycongen biosynthesis.

글라이포세이트-계면활성제 제초제로 유발된 백서의 저혈압모델에서 지방에멀젼정맥주사(intravenous fat emulsion)의 효과에 대한 선행연구

조별님희,유인술,안홍준,김건동,김훈,유승 대한응급의학회 2013 대한응급의학회지 Vol.24 No.6

Purpose: Decontamination and supportive care are general types of treatment for glyphosate surfactant herbicide (GlySH) intoxication. However, no particular treatment for refractory shock has been established as a conventional therapy. Therefore, this study examined whether intravenous fat emulsion therapy (IFE) is effective on GlySHinduced shock. Methods: This preliminary study was conducted on 10 rats. After anesthesia and catheterization, shock was induced by GlySH infusion. After a stabilization period, animals were randomized to receive intravenous normal saline (Group 1)or 20% lipid emulsion (Group 2). Results: In the shock state, there was no significant difference between the two groups for systolic blood pressure (SBP), diastolic blood pressure (DBP), and mean arterial pressure (MAP). In the post-management state, there was no significant difference between the two groups for SBP and MAP. However, there was a significant difference for DBP (Group 1, median 72.5 (IQR 52.8-74.6) mmHg; Group 2, median 77.6 (IQR 74.8-98.3) mmHg, p=0.016), SBP variation (Group 1, median 5.5 (IQR 1.6-11.1) mmHg; Group 2,median 25.6 (IQR 15.5-42.9) mmHg, p=0.008); DBP variation (Group 1, median -1.02 (IQR -4.69-11.0) mmHg; Group 2, median 21.1 (IQR 14.0-43.2) mmHg, p=0.008); MAP variation (Group 1, median 1.15 (IQR -2.6-11.0) mmHg;Group 2, median 23.2 (IQR 14.2-42.8) mmHg, p=0.008). Conclusion: The IFE increased blood pressure when shock was induced using GlySH. However, further studies are required to determine what components induced the shock and permitted a response to the IFE.

Nasal Hyperreactivity to Methacholine Test in Perennial Allergic Rhinitis

조중생,이건희,김훈,유영인,차창일 대한비과학회 2001 Journal of rhinology Vol.8 No.1

Although histamine and methacholine challenge tests are commonly used to evaluate the hypersensitivity in allergicrhinitis, some controversies still remain to standardize the test. This study was designed to evaluate the usefulness of themethacholine challenge test for the evaluation of hyperreactivity in allergic rhinitis. Twenty three patients with perennialallergic rhinitis and 17 normal subjects were included in this study. All subjects underwent saline nasal challenge andmethacholine nasal challenge test with different doses (6 mg, 12 mg, 24 mg / in 0.5 ml of saline). We measured the nasalcavity volume by acoustic rhinometry in each step and counted the number of sneezes for 15 minutes. The volume of nasalsecretion was also measured by tissue paper method. Besides that, we compared the data between allergic patients with ashort (under 1 year) and long duration (more than 1 year). There were no differences in the frequency of sneezing andnasal volume change to each amount of methacholine in both groups. Nasal secretion increased after methacholine challengein both groups. However, the allergic group showed significant increase after the methacholine challenge of 12 mg comparedto the control group. And the volume of nasal secretion significantly increased in patients with a long duration. The nasalmethacholine challenge test can be used as a diagnostic tool to detect hyperreactivity in perennial allergic rhintis. The optimaldilution was 12 mg (in 0.5 ml of saline) for the nasal methacholine challenge test.

Cloning and Identification of Essential Residues for Thermostable β-glucosidase (BgIB) from Thermotoga maritima

홍수영,조계만,김용희,홍선주,조수정,조용운,김훈,윤한대,Hong, Su-Young,Cho, Kye-Man,Kim, Yong-Hee,Hong, Sun-Joo,Cho, Soo-Jeong,Cho, Yong-Un,Kim, Hoon,Yun, Han-Dae Korean Society of Life Science 2006 생명과학회지 Vol.16 No.7

A hyperthermophilic bacterium Thernotoga maritima produced thermostable ${\beta}-glucosidase$. The gene encoding ${\beta}-glucosidase$ from T. maritima MSB8 was cloned and expressed in Escherichia coli. The en-zyme (BgIB) hydrolyzed ${\beta}-glucosidase$ linkages between glucose and alkyl, aryl of saccharide groups such as salicin, arbutin, and $_pNPG$. The insert DNA contained ORF with 2,166 bp encodes a 721 amino acids (calculated molecular mass of 80,964 and pl of 4.93). The amino a.id sequence of BglB showed the similarity to family 3 glycosyl hydrolases. The molecular weight of the enzyme was estimated to be approximately 81kDa by MUG-nondenaturing PAGE (4-methylumbelliferyl 13-D-glucoside-nondenaturing polyacrylamide gel electophoresis) and SDS-PACE. The ${\beta}-glucosidase$ exhibited maximal activity at pH 7.0 and $80^{\circ}C$. By exchanging two possible residues (Glu-232 and Asp-242) to Ala by site-directed mutagenesis method, it was found that these were essential for enzymatic activity. 초고온성 세균인 Thermotoga maritima로부터 ${\beta}-glucosidase$ 유전자를 클로닝한 후 대장균 숙주에서 발현시켰다. 이 효소는 salicin, arbutin, $_pNPG$과 같은 탄소원의 ${\beta}$-글루코시드 결합을 가수분해하였다. 721개의 아미노산을 암호화하는 2,166 bp의 DNA 염기서열로된 유전자이였다. 다른 ${\beta}-glucosidase$ 효소들과 단백질 유사성을 비교한 결과 glycosyl hydrolase family 3에 속하였으며 MUG-nondenaturing PAGE와 SDS-PAGE에 의해 확인된 단백질의 크기는 약 81 kDa이었다. 효소활성은 pH 7.0, $80^{\circ}C$에서 가장 높은 활성을 나타냈으며 이 효소의 아미노산 서열에 있는 두 개의 아미노산 잔기 (232번 글루탐산과 242번 아스파르트산 잔기)를 알라닌으로 치환시켜 활성이 없어지는 것으로 보아 이 두 잔기가 효소활성에 중요한 역할을 하는 것으로 추정된다.

상악동에 국한된 섬유성 이형성증 1예

이건희,조중생,이인영,김훈 대한이비인후과학회 2002 대한이비인후과학회지 두경부외과학 Vol.45 No.1

Fibrous dysplasia is a developmental disease of the bone, characterized by the replacement of normal spongiosa and filling of the medullary cavity of affected bones by an abnormal fibrous tissue. Fibrous dysplasia arising in the paranasal sinus is are predominant:the monostotic type, in which only one bone is afected;and the polyostotic type, in which multiple bo-nes are affected. Recently, we experienced a case of monostotic fibrous dysplasia which was confined to the maxillary sinus and approached by the Caldwell-Luc method. Hence, we report a case of fibrous dysplasia of the maxillary sinus with a re-view of literature. (Korean J Otolaryngol 2002;45:86-8)