Aldose reductase inhibitory activity of the methanol extracts from Korean folk plants

김혜민,한샘,최경,구자정,박광우,조은주,이상현,Kim, Hye-Min,Han, Saem,Choi, Kyung,Ku, Ja-Jung,Park, Kwang-Woo,Cho, Eun-Ju,Lee, Sang-Hyun Institute of Agricultural Science 2012 Korean Journal of Agricultural Science Vol.39 No.2

To search for the aldose reductase (AR) inhibitors from Korean folk plants, the inhibition of rat lens AR in vitro using the methanol (MeOH) extracts from Korean folk plants was investigated. Among fifty four Korean folk plants tested, the MeOH extract of Cedrela sinensis showed highest inhibition of AR ($IC_{50}$ value, 2.52 ${\mu}g/ml$). The plant C. sinensis has a possibility of new natural resources for the development of AR inhibitor for the prevention of diabetic complications.

불안장애의 치료추구시간에 대한 공존 정신질환의 영향 : 사회불안장애와 공황장애 간 비교

김혜민,하주원,임세원,오강섭,Kim, Hye-Min,Ha, Juwon,Lim, Se-Won,Oh, Kang-Seob 대한불안의학회 2012 대한불안의학회지 Vol.8 No.2

Objective : Individuals with anxiety disorders experience a wide range of time to seeking treatment (TST) as well as various comorbid mental disorders. The present study examined the TST in social anxiety disorder (SAD) and panic disorder. This study aimed to find out the influence of comorbid mental disorder on TST of anxiety disorder through the comparison of SAD and panic disorder. Methods : This study included 311 SAD and 378 panic disorder patients at the initial visit of psychiatric clinic. Contribution of clinical (number of comorbidity, comorbid type and onset age) and demographic (current age) factors to TST were investigated by multivariate analysis. Results : The median length of TST was 14.03 years in SAD and 2.26 years in panic disorder. In social anxiety disorder, fewer comorbidity, younger onset age, and older age were factors associated with delayed TST. In panic disorder, only younger onset age was associated with delayed TST. In both disorders, comorbid depressive disorder was associated with shorter TST. Conclusion : Our data provided the differences in illness behavior needing help based on comorbid mental disorders between SAD and panic disorder. In addition to comorbid disorder, factors affecting TST of anxiety disorder requires future investigation.

한국인 조기난소부전 여성의 배란양상

김혜민,김재령,최은주,박은주,홍서유,이진용,박원일,Kim, Hye-Min,Kim, Jae-Ryoung,Choi, Eun-Joo,Park, Eun-Joo,Hong, Seo-Yu,Lee, Jin-Yong,Park, Won-Il 대한생식의학회 2008 Clinical and Experimental Reproductive Medicine Vol.35 No.3

Objective: Significant portion of premature ovarian failure patients ovulate intermittently and even can achieve pregnancy. The clinical features and reproductive potentials of Korean women with premature ovarian failure have never been elucidated. The purpose of this study is to assess ovarian follicular growth and ovulatory potential. Methods: Complete medical records of fifteen women with idiopathic premature ovarian failure were reviewed retrospectively. Subjects with more than 6 ultrasonographic scans and 36 months of follow-up period were included. Once the ovarian follicle was found by ultrasonography, the follicular growth was serially monitored. The result of ovulation induction of 6 cycles in 4 patients were also evaluated. Rate of positive ovarian follicle by ultrasound, rate of natural follicular growth and response of ovulation induction were assessed. Results: Among the 15 patients, 6 women (40%) had ovarian follicle in random ultrasonography. But the chance of positive follicle per ultrasonography scan was 7% (8 out of 115 scans). One out of 6 women eventually ovulated during the follow-up scan. In remaining 5 subjects, size of follicles remained unchanged. None of 6 ovulation induction cycles resulted successful ovulation. Conclusion: Incidence of positive ovarian follicle in Korean premature ovarian failure patients is lower than that of Western counterpart. This result, however, could not be directly compared because of the differences of study design. The ovulation rate by medical ovulation induction in this group is 0%, which is worse than the reported success rate of Western studies. 목 적: 조기난소부전 환자의 10$\sim$20%에서 난소기능이 회복될 수 있다고 하며 간헐적으로 배란이 되고 심지어는 임신이 가능할 수 있다는 보고가 있다. 그러나 한국의 조기난소부전 여성의 임상적 특징과 배란가능성은 논의된 적이 없다. 이 연구의 목적은 한국인 조기난소부전 환자에서 자연 난포의 발생 빈도와 배란유도의 가능성을 평가하는 것이다. 연구방법: 특발성 조기난소부전으로 진단된 15명 여성의 전체 진료기록을 후향적으로 연구하였다. 환자별로 6번 이상의 초음파 검사를 시행하였고 36개월 이상의 기간 동안 추적관찰을 하였다. 또한 4명의 환자에서 6주기의 배란유도시도를 하였다. 조사항목은 초음파 검사에서 난포의 발견율, 자연 난포 성장률과 배란유도를 시행한 경우에 그 반응을 평가하여 결과를 분석하였다. 결 과: 15명 중 6명은 초음파상 난포가 발견되었나 (40%) 초음파 검사 당 난포 발견 빈도는 7%였다 (115회 중 8회). 6명 중 한 명은 초음파 추적관찰 상 배란이 확인되었고 나머지 5명은 크기에 변화가 없었다. 배란유도를 시도한 6명 중 성공적인 난포 성장이 관찰된 예는 없었다. 결 론: 한국인 조기난소부전 환자에서 난포의 발견율은 이전 서양 연구에 비하여 낮다고 생각되지만 관찰 방법에 차이가 있어 직접비교는 불가능하다. 배란유도의 성공율은 0%로서 이전 연구에서 발표된 수준보다 낮았다.

Lignan contents in Acanthopanax senticosus by HPLC

김혜민,조선행,국순자,이상현,Kim, Hye-Min,Cho, Seon-Haeng,Kook, Soon-Ja,Lee, Sang-Hyun Institute of Agricultural Science 2011 Korean Journal of Agricultural Science Vol.38 No.3

A reverse-phase system of HPLC using a linear gradient of acetonitrile and deionized water was developed for the quantification lignans, eleutherosides B and E, in Acanthopanax senticosus. The HPLC system consisted of linear gradient of acetonitrile and deionized water, and UV/VIS detection was set at 210 nm. Both eleutherosides B and E contents in different parts of A. senticosus were determined. As a result, the contents of eleutherosides B and E were measured in the leaves (trace amounts and 0.029 mg/g, respectively), stems (0.107 and 1.015 mg/g, respectively), roots (0.026 and 0.390 mg/g, respectively), and fruits (0.022 and 0.043 mg/g, respectively). Moreover, eleutherosides B and E in the water extract were found 0.011 and 0.171 mg/g, respectively.

분자동역학법에 의한 기액계면 분자의 운동특성에 관한 고찰

김혜민,박권하,최현규,최순호,Kim Hye-Min,Park Kweon-Ha,Choi Hyun-Kue,Choi Soon-Ho 한국마린엔지니어링학회 2005 한국마린엔지니어링학회지 Vol.29 No.1

An experimental study of molecular motions on a liquid-vapor interface is limited due to micro-scale characteristics of a system with an angstrom or a nanometer size Therefore, in recent, many studies for micro-scale systems have been conducted by a computer simulation because it is free from experimental limitations. In this study, through the molecular dynamic (MD) method. molecular behavior was clarified on a liquid-vapor interface and a criterion to distinguish between liquid and vapor was suggested by a potential energy and the number of neighboring molecules. At an interface. the potential energy of a molecule was increased but the number of neighboring molecules was decreased when the molecule moved into a vapor region from a liquid region, and vice versa.

기술사업화 정책 변화 방향 예측에 관한 연구

김혜민(Hye-Min Kim),한정희(Jung-Hee Han),김연배(Yeon-Bae Kim) 한국산업경제학회 2013 산업경제연구 Vol.26 No.2

본 연구는 미래 기술사업화 정책을 예견하여 기업에게 기술사업화 활동의 방향성을 찾아보는데 목표를 두었다. 연구에서는 먼저, 미래사회의 큰 변화 트렌드를 파악하기 위해 거시환경분석 기법인 STEEP 분석기법과 계층적 의사분석방법인 AHP 분석을 실시하였다. 미래 기술사업화 정책에 영향을 주는 요인으로 STEEP 분석에서는 기술적 요인에 대한 가중치가 가장 높았고 이어 경제적 요인, 환경적 요인, 정치적 요인, 사회적 요인이 뒤를 이었다. 하위 판단기준을 종합해 보면, ‘기술 및 사업의 융복합 현상 가속화’, ‘그린, 친환경분야 수요 증가’, ‘세계 경제의 통합’, ‘탈추격형 기술혁신 필요성 증대’ 순으로 분석되었다. AHP 분석에서는 Open Innovation 및 융복합 기술육성 정책이란 대안이 1순위로, 지식재산권 보호 정책이 2순위로, 공여형(ODA) 기술이전이 3순위였다. 또한 사회 환경 변화 방향에서는 실버산업, 바이오기술 산업 등 관련 기술 분야의 투자 강화가 예견되었다. 이와 같은 결과를 토대로 볼 때, 개별기업은 미래에도 기업의 핵심 지식과 기술은 보호하면서도 외부자원을 적극 활용하는 전략이 요구된다. 기술사업화를 통한 지속적 성장을 위해서 개별기업은 적정기술들의 신흥국가의 공여와 함께, 실버와 친환경 분야 기술사업화에도 관심을 같은 것이 필요하다 판단된다. This study explores the future trend of technology commercialization activities for each firms by foreseeing the technology commercialization policy trends. With a view of the purpose, STEEP analysis and AHP(Analytic Hierarchy Process) analysis are used. According to the findings, as factors, which influence the policy change in the future, technology factors are the more important than other four factors. In AHP survey analysis, the result of the future direction of technology transfer and commercialization policy change appears not to upset the existing policy issues and implementations. This implies that current technology commercialization policy direction focusing on open innovation and globalization, technological convergence are the right track as well as the prospective outlook reflecting global macro-environments and future issues. This study has meaningful in the perspectives that we can attempt to manage the uncertainty and sudden changes of the future by presenting the future direction of technology transfer and commercialization policy which is designed to cope with the high possibilities and major issues that are drawn by future analysis. Also each firms are more interested in as followed; 1) having a current stance of open innovation activities for technology commercialization and have a balance with technology strategy between giving and taking 2) protecting Intellectual property 3) giving matured technologies to developing counties 4) considering social changes.

초임계 이산화탄소 처리가 동결 건조된 소간의 이화학적 특성 및 미생물 저감화에 미치는 영향

김혜민(Hye-Min Kim),우성운(Sung-Woon Woo),김아나(Ah-Na Kim),허호진(Ho-Jin Heo),천지연(Ji-Yeon Chun),최성길(Sung-Gil Choi) 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.12

본 연구에서는 초임계 이산화탄소 처리에 따라 동결 건조된 소간의 이화학적 특성과 미생물 저감화 효과를 연구하였다. 다양한 압력(200, 300 및 450 bar)으로 초임계 이산화탄소를 처리한 결과, 먼저 지방 추출 수율의 경우 200, 300, 450bar에서 각각 84, 86, 90%로 나타나 처리 압력이 높아짐에 따라 증가하는 경향을 보였다. 비타민 A 및 CoQ10 성분은 지용성 물질로서 거의 대부분 초임계 이산화탄소 처리에 의해 다른 지방성분과 같이 추출된 것으로 나타났다. 추출된 지방의 지방산을 분석한 결과, 포화지방산 비율은 처리 압력이 높아질수록 감소한 반면에 다가불포화지방산은 증가하는 경향을 보였다. 총 아미노산의 경우 초임계 이산화탄소를 처리한 시료군에서 대조군에 비해 낮은 함량을 보였다. 총세균수의 경우 동결 건조로 인해 수분 함량이 극히 낮아졌음에도 불구하고 처리 시간이 길어질수록 저감화되었으며, 또한 5일간 냉장 저장한 시료에서 총세균수가 약 6.2 log CFU/g이었으나 100 bar에서 3시간 처리에 의해 약 4.4 log CFU/g으로 저감화되었다. 특히 흥미롭게도 대장균군의 경우 100 bar에서 3시간 처리 후 저장 시 7일차까지 전혀 검출되지 않았다. 본 결과는 초임계 이산화탄소 처리가 수분 함량이 낮은 식품소재의 대장균군 저감화에도 효과적일 수 있다는 것을 나타내는 것이라 사료된다. 결론적으로 초임계 이산화탄소의 적절한 처리를 통해 미생물 안전성 및 지방 산화에 대한 안정성이 개선된 소간을 고단백 식품소재로 활용할 수 있으리라 판단된다. Supercritical carbon dioxide (SC-CO₂) treatment has been becoming an important method for substituting the use of organic solvents for samples extraction prior to analysis due to its low toxicity, ease of handling, low cost of disposal etc. Freeze-dried bovine liver was treated with SC-CO₂ under different pressures (200, 300, and 450 bar) in order to investigate effects on physicochemical properties and reduction of microbial load. The yield of lipid extraction from bovine liver by SC-CO₂ treatment increased with increasing pressure, with values of 84, 86, and 90% in response to 200, 300, and 450 bar, respectively. Results of high performance liquid chromatography analysis showed that vitamin A and coenzyme Q10 (CoQ10), which is soluble in lipid, were almost removed from bovine liver by SC-CO₂ treatment. Saturated fatty acids ratio of bovine liver decreased with increasing pressure, whereas polyunsaturated fatty acids increased with increasing pressure. Total content of amino acids in bovine liver treated by SC-CO₂ was less than that of the control sample without treatment. The number of aerobic bacteria in bovine liver, which was stored at 5°C for 5 days and freeze-dried, decreased from 6.2 to 4.2 log CFU/g by SC-CO₂ treatment at 100 bar for 3 h. Interestingly, coliform bacteria were not found in the bovine liver sample by SC-CO₂ at 100 bar for 3 h under all storage conditions. This indicates that SC-CO₂ treatment can effectively reduce coliform bacteria in the food matrix even at low moisture. In conclusion, freeze-dried bovine liver by proper SC-CO₂ treatment may be used as a potential high protein source, with increasing microbial safety and stability of lipid oxidation.

탈미네랄골분이 첨가된 알지네이트 스펀지에서 조직공학적 연골 재건

김혜민(Hye Min Kim),박진영(Jin Young Park),김은영(Eun Young Kim),송정은(Jeong Eun Song),권순용(Soon Young Kwon),정진화(Jin Wha Chung),강길선(Gil Son Khang) 한국고분자학회 2014 폴리머 Vol.38 No.3

알지네이트와 탈리네랄골분(DBP) 하이브리드 스펀지는 각각 DBP가 0, 10, 20, 40 및 80% 첨가된 스펀지로, 연골 재건을 위해 제작되었다. 세포의 증식은 MTT를 통해 관찰했으며, 형태학적, 조직학적, 생물학적 관찰 그리고 RT-PCR을 1,2, 및 3주에 실시하였다. 세포생존율에 있어서 20% dbp/알지네이트 스펀지에서 다른 스펀지보다도 가장 좋은 결과를 보였으며, 광학전자현미경 관찰에서 또한 20% 스펀지에서 연골세포의 표현형이 가장 잘 유지됨과 동시에 산성뮤코다당류와 제2형 콜라겐이 잘 형성된 것을 확인하엿다. 이로써 DBP/알지네이트 하이브리드 스펀지는 연골 조직공학을 휘한 지지체로써 활용될 수 있을 것이다. Demineralized bone particles (DBP) and alginate hybrid sponges were fabricatid at 10, 20, 40 and 80% DBP/alginate hybrid ratios for seeding chondrocyte. Cell proliferation was measured via MTT assay, Morphological obser-vatin, histology, biological assay and RT-PCR were performed at each time point 1,3, and 3 weeks, The cell viability was better in 20% DBP/alginate sponges than in other sponges, SEM results showed that more attachesd and more pro- liferated cells in the 20% dbp/DBP/alginate sponges with the lapse of time. Finally, histochemical assay results showed that the phenotype of chondrocyte was well maintained and both acidic muecpolysaccharide and type II collagen was well formed at 20% sponges. This study suggested that DBP/alginate sponge may serve as a potential cell delivery vehicle and a structural basis for tissue engineered articular cartilage.

Aldose reductase inhibitory activity of the methanol extracts from Korean folk plants

Hye Min Kim(김혜민),Saem Han(한샘),Kyung Choi(최경),Jajung Ku(구자정),Kwang-Woo Park(박광우),Eun Ju Cho(조은주),Sanghyun Lee(이상현) 충남대학교 농업과학연구소 2012 농업과학연구 Vol.39 No.2

To search for the aldose reductase (AR) inhibitors from Korean folk plants, the inhibition of rat lens AR in vitro using the methanol (MeOH) extracts from Korean folk plants was investigated. Among fifty four Korean folk plants tested, the MeOH extract of Cedrela sinensis showed highest inhibition of AR (IC50 value, 2.52 ㎍/ml). The plant C. sinensis has a possibility of new natural resources for the development of AR inhibitor for the prevention of diabetic complications.

동절기 수박 접목묘의 안정적 생산을 위한 나노탄소섬유적외선 램프의 난방효과에 대한 적정 설치간격

김혜민(Hye Min Kim),정현우(Hyeon Woo Jeong),황희성(Hee Sung Hwang),황승재(Seung Jae Hwang) (사)한국생물환경조절학회 2022 생물환경조절학회지 Vol.31 No.1

본 연구는 동절기 공정육묘장의 수박 플러그 묘 생산 시, 난방 에너지 절감에 유리한 난방장치인 나노탄소섬유 적외선 난방등의 효율적인 사용을 위한 적정 전력과 설치 간격을 구명하기 위해 수행되었다. 유리온실 내 소형 베드 6개를 플라스틱 필름으로 구획을 나누었으며, 100cm 높이에 700W와 900W 난방등을 설치하였다. 난방등은 설치 간격을 각각 중앙 1개(Control), 60cm 간격(2개), 40cm 간격(3개)으로 설치하였다. 나노탄소섬유 적외선 난방등을 점등한 후 대조구를 제외한 모든 처리에서 설정한 야간 기온(20℃)을 유지하였다. 엽온은 난방등 설치 간격이 좁을수록 빨리 상승하는 경향을 보였다. 나노탄소섬유 적외선 난방등을 사용함에 따른 처리구간 수박 접목묘의 생육에는 뚜렷한 차이가 없었으나, 엽장과 엽폭은 700W 난방등에서 설치 간격이 짧을수록 길어지는 경향을 보였으며, 700W 난방등을 40cm 간격으로 설치하였을 때 묘의 충실도가 높은 값을 나타내는 것을 확인할 수 있었다. 따라서 야간 기온 유지와 묘의 충실도를 고려하였을 때, 700W 난방등을 40cm 간격으로 설치하는 것이 수박 접목묘를 생산하는데 경제성을 고려하여 효율적인 나노탄소섬유 적외선 난방등의 전력과 설치 간격으로 판단된다. This study was carried out to investigate the proper wattage and installation distance for the efficient use of nano-carbon fiber infrared heating lamp (NCFIHL), a heating device advantageous for heating energy saving, when the production of watermelon plug seedlings in the plug seedling nursery in winter season. Six small beds were divided into plastic film, and 700 W and 900 W nano-carbon fiber infrared heating lamps were installed at 100 cm above the bed. 1 lamp at central (control), 60 cm interval (2 lamps), and 40 cm interval (3 lamps) heating lamps were installed in each bed inside the greenhouse. All treatments, except the control, were set to keep the night air temperature at 20℃ after lighting the NCFIHL. The leaf temperature showed a tendency to increase fast as the install distance was narrow. The leaf length and leaf width tended to increase as the installation distance of the 700 W heating lamp was narrow. The compactness was high in 700 W heating lamp with 40 cm of installation distance. Therefore, in consideration of maintaining the set temperature at night, installing 700 W electric lamps at 40 cm was an efficient power and installation distance for watermelon grafted seedlings considering economic feasibility.