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An Information-based Model for an Interactive Web Service with Agricultural Biotechnology
김창국,설영주,박동석,한장호 한국유전체학회 2011 Genomics & informatics Vol.9 No.2
The National Agricultural Biotechnology Information Center (NABIC) constructed an agricultural biology-based infrastructure and developed a biological informationbased database. The major functions of the NABIC are focused on biotechnological developments for agricultural bioinformatics and providing a web-based service to construct bioinformatics workflows easily, such as protein function prediction and genome systems biology programs. The NABIC has concentrated on the functional genomics of major crops, building an integrated biotechnology database for agro-biotech information that focuses on the proteomics of major agricultural resources,such as rice, Chinese cabbage, rice Ds-tagging lines, and microorganisms.
Genetic Analysis of Seed-specific Gene Expression for Pigmentation in Colored Rice
김창국,Shoshi Kikuchi,Kouji Satoh,김진아,김동헌,김용환,박성한,윤웅한,이점호 한국바이오칩학회 2009 BioChip Journal Vol.3 No.2
Anthocyanin is a major class of flavonoids that tissues produce in response to environmental signals. The pigmentation of colored rice was analyzed by oligo microarray based on two factors, cultivar and developmental stage. As many as 250 and 350 genes were identified to be significantly up- and down-regulated. Using hypergeometric analysis for transcription factor function, the Myb and GT families, PBP, PBF, RAV, and STF factors were identified have potential anthocyanin-specific functions. We also obtained seventeen unknown genes which display novel functions. Among the genes within the GT-1 and Myb1 groups, three unknown genes were found to be upregulated significantly in both combinations of cultivar and developmental stage. These results showed functional diversity of TF families and that the biological functions of the particular TFs may be activated in a pigmentation pathway in rice.
김창국,이대연,이영철,Shim, Jae-Kun,Junfeng Huang 한국사회체육학회 2007 한국사회체육학회지 Vol.0 No.31
Development of Muscle Force Generation and Force Control ofHand Digit:Age-related Changes
An Integrated Database and Web Service for Microbial Resources at KACC
김창국,전영아,조규택,권순우,김용환,홍승범 한국유전체학회 2009 Genomics & informatics Vol.7 No.1
The Korean Agricultural Culture Collection (KACC) has developed a web-based system to provide an integrated database with information updates about microbial resources. This integrated database consists of 5 major functions and contains general information, which includes identification numbers, culture media composition, image information, DNA sequences, patent information, and general forms for ordering and depositing microorganisms. In 2008, KACC started providing characterization information. KACC maintains 9,801 cultures of microorganisms, including 3,296 strains of bacteria, 4,734 fungi, 784 actinomycetes, 64 yeasts, and 923 others.
Computational identification of Chinese cabbage anthocyaninspecific genes
김창국,김진아,Shoshi Kikuchi,최지원,김용갑,박현주,설영주,박동석,한장호,김용환 한국바이오칩학회 2011 BioChip Journal Vol.5 No.2
Therefore, 3,895 candidate genes related to anthocyanin biosynthesis in Chinese cabbage were identified using a 300K Brassica rapa microarray analysis. Gene expression during six stages of leaf developmental stages were examined in FC (green leaf) and FA(red leaf) Chinese cabbage cultivars. The 317 transcription factor genes found to be associated with anthocyanin were classified into 11 functional groups. The ratio of expression levels of each transcription factor between the two cultivars was examined during the six leaf developmental stages. A total of 14 genes were found to be expressed in all developmental stages commonly. Among these genes, 10 unknown and hypothetical genes were differentially revealed to be expressed between the two cultivars at each developmental stage, as determined by microarray analysis, and were verified by RT-PCR validation. These genes most likely play regulatory roles in either anthocyanin production or metabolism during flavonoid biosynthesis. While these genes require further validation and characterization, our results illustrate the potential usefulness of this multi-layered screening method using Chinese cabbage (Brassica rapa) microarrays.