기관지 내시경으로 초기에 제거할 수 없었던 기도 이물 : 2례 보고

김연수,남승연,곽병곤,장우익,박경택,김창영,류지윤,Kim, Yeon-Soo,Nam, Seung-Yeon,Kwak, Byeong-Gon,Chang, Woo-Ik,Park, Kyung-Taek,Kim, Chang-Young,Ryoo, Ji-Yoon 대한기관식도과학회 2007 大韓氣管食道科學會誌 Vol.13 No.2

Foreign body aspiration is a cause of the accidental death at home. Therefore, early intervention and proper management is important. A bronchoscopy is indicated whenever there is a suggestive history and medical opinion. Occasionally, foreign body removal with bronchoscopy may be fail. But, on the situation, there is no definite recommended standard management. We experienced two cases of bronchial foreign body could not be removed with bronchoscopy at first intervention. The one was diagnosed too late. Endobronchial granulation tissue and edema made it impossible to find the foreign body at first bronchoscopy. After steroid and antibiotic therapy, foreign body could be removed with secondary bronchoscopy. Another was bronchial foreign body jammed tightly bronchus intermedius. Even after medical therapy, patient got aggravated. So foreign body was removed with bronchotomy.

강원도 사육 한우의 간질 감염실태

김연수,김상균,황의경,Kim, Yeon-Soo,Kim, Sang-Kyun,Hwang, Eui-Kyung 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.4

A field survey of fascioliasis of Korean native cattle raising and raised in specialized commercial breeding farms and local farms in Kangwon province using both intradermal test and sedimentation technique for feces was carried out from November to December, 1996. Fecal samples were taken from fascioliasis positive cattle by the intradermal test for the fecal examination. Liver tissues were randomly collected from an abattoir for histopathological examination of liver fluke infection in cattle. The results are as follows. 1. By the intradermal test for a total of 211 cattle raising in both Wonju and Wheongsung, Kangwon province, 60 heads(28.4%) showed positive reaction. Among 60 positive cattle, eggs of Fasciola hepatica were found from 51 heads(85.0%) by sedimentation technique. 2. According to the cattle raising areas, the positive rates by the intradermal test were 26.7%(20 out of 75 heads) in Wonju and 29.4%(40 out of 136 heads) in Wheongsung. 3. According to the age of cattle examined, the positive rates by the intradermal test in 1~3, 4~6 and 7~10 years old were 11.7%(7 out of 100 heads), 68.3%(41 out of 93 heads) and 20.0%(12 out of 18 heads), respectively. 4. The overall infection rates of fluke larvae from the slaughtered cattle at an abattoir in Wonju was 24.7%(37 out of 150 heads). In histopathology, liver lesions were observed such as inflammation with infiltration of eosinophils, polymorphonuclear cells, mononuclear cells and multinucleated giant cells, proliferation of connective tissues, calcification and abscess formation.

완전삭제 도구 사용 흔적에 관한 연구

김연수,방제완,김진국,이상진,Kim, Yeon-Soo,Bang, Je-Wan,Kim, Jin-Kook,Lee, Sang-Jin 한국정보처리학회 2010 정보처리학회논문지 C : 정보통신,정보보안 Vol.17 No.2

The data wiping is a technique which perfectly deletes data in a storage to prevent data recovery. Currently, management of stored data is important because of increasing an accident of personal information leakage. Especially, if you need to discard data contained personal information, using a wiping tool which permanently deletes data to prevent unnecessary personal information leakage. The data wiping is also used for data security and privacy protection. However the data wiping can be used intentionally destruction of evidence. This intentionally destruction of evidence is important clues of forensic investigation. This paper demonstrates the methods for detecting the usage of wiping tools in digital forensic investigation. 완전삭제 기술은 저장장치 내 데이터를 복구가 불가능하도록 흔적 없이 완벽하게 삭제하는 기술이다. 최근 개인정보 유출 사고가 급증함에 따라 저장된 데이터의 관리가 중요해지고 있다. 특히 개인정보가 포함된 데이터를 폐기해야 하는 경우, 데이터를 영구적으로 삭제하는 완전삭제 도구를 사용함으로써 개인정보에 대한 불필요한 유출을 막을 수 있다. 또한 완전삭제 기술은 데이터 보안 및 프라이버시 보호 측면으로 활용 가능하다. 그러나 완전삭제 기술은 의도적으로 사건과 관련된 증거를 인멸하기 위해 사용될 수도 있다. 이러한 의도적인 증거 인멸은 사건 수사에 있어서 중요한 실마리가 될 수 있다. 본 논문에서는 디지털 포렌식 수사 과정에서 완전삭제 도구의 사용 흔적을 확인할 수 있는 방안을 제시한다.

식도이완불능증에시 복강경을 이용한 식도근절개술 및 전방위저주름술 - 1예 보고 -

김연수,류지윤,Kim Yeon Soo,Ryoo Ji Yoon 대한흉부심장혈관외과학회 2005 Journal of Chest Surgery (J Chest Surg) Vol.38 No.3

An 18-year old woman had dysphagia and frequent vomiting after meals for 6 years. She lost 15 kg in 6 months recently. After esophageal manometry, she was diagnosed with achalasia. We decided to use laparoscopic surgery because there was no symptomatic improvement after medication. We made small 5 incisions on her abdomen. We performed Heller myotomy and Dor fundoplication. We performed esophagogram one day after the operation. There was no leakage of the contrast media, and it passed well. She started to eat at the 2nd day after the operation and was discharged on the 9th day without other specific problem. 환자는 18세 여자로 6년 전부터 연하곤란 및 식사 후 빈번하게 구토가 발생하였으며, 최근 6개월 동안 15 kg의 체중 감소가 있었다. 식도 계측검사에서 식도이완불능증으로 진단되었다. 약물치료를 하였으나 증상이 호전되지 않아 복강경을 이용한 수술을 결정하였다. 복부에 5개의 트로카용 소절개를 시행하였고, 복강경을 이용하여 근절개술과 전방위저부주름술을 시행하였다. 수술 후 1일째 식도조영술을 시행하였다. 조영제가 누출 없이 원활하게 통과되었다. 수술 후 2일째 식이를 시행하였으며, 술 후 9일째 특별한 문제없이 퇴원하였다.

DNA Amplified Fingerprinting 기법을 이용한 Salmonella pullorum과 Salmonella gallinarum의 다형성 비교 분석

김연수,김상균,송원철,황의경,Kim, Yeon-Soo,Kim, Sang-Kyun,Song, Won-Chul,Hwang, Eui-Kyung 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.3

This study was performed to detect the Salmonella genus-specific DNA marker for comparing of polymophisms between S pullorum and S gallinarum by using PCR amplified techniques. A total of ten primers were used to detect DNA polymorphisms from S pullorum and S gallinarum. The number of DAF bands detected per each primer varied from 26 to 45, with an average of 32.7 using 10 primers. A total of 327 DAF bands were generated and among them 123 bands were polymorphic(37.6%). These DNA amplified fingerprinting(DAF) specific bands for S pullorum and S gallinarum were observed from all primers. For S pullorum, GEN 60-04, GEN 70-04 and GEN 70-03 primers showed a high level of polymorphism with 0.79, 0.70 and 0.57, respectively. But GEN 60-05 primer did not show a level of polymorphism. For S gallinarum, GEN 70-03, 60-04, 60-07, 70-05 and 70-04 primers showed a higher a low level of polymorphism from 0.16 to 0.28. Each five strains of S pullorum and S gallinarum were isolated from chickens showed typical clinical signs related with infection of pullorum disease or fowl typhoid at commercial chicken farms. DNA markers of these strains produced by GEN 70-04, GEN 70-05 and GEN 70-08 showed significant difference of band patterns between S pullorum and S gallinarum. These DNA markers could be used for comparison of DNA marker polymorphism between S pullorum and S gallinarum as well as rapid diagnosis of fowl typhoid and pullorum disease of domestic fowls.

금은화산(金銀花散)의 LPS로 유도된 Raw 264.7 세포에서의 항염증 효과

김연수,김수진,지선영,황보민,Kim, Yeon-Soo,Kim, Su-Jin,Jee, Seon-Young,Hwangbo, Min 대한한방안이비인후피부과학회 2021 한방안이비인후피부과학회지 Vol.34 No.3

Objectives : The purpose of this study is to investigate the anti-inflammatory effect of Geumeunwha-san(GEHS) water extract in vitro. Methods : To evaluate the anti-inflammatory effect of GEHS, Raw 264.7 cells were pretreated with 10-300㎍/㎖ of GEHS for 1hr, and then exposed to 1㎍/㎖ of LPS. MTT assay was used to detect the cell viability. Productions of pro-inflammatory cytokines and NO were measured in culture media. By using immunoblot analysis, protein levels of iNOS and NF-𝜅B were determined. Results : In vitro study, cell viability assay on GEHS treatment of 10-300㎍/㎖ has no cytotoxicity in Raw 264.7 cells. Pretreated 100, 300㎍/㎖ of GEHS had significantly inhibited LPS-induced NO production. And also pretreatment of 100, 300㎍/㎖ GEHS had significantly decreased production of interleukin-6, -1𝛽 and tumor necrosis factor-𝛼 in LPS-activated Raw 264.7 cells. In addition, GEHS reduced LPS-mediated iNOS expression. Moreover I-𝜅B𝛼 expression was significantly induced by GEHS and NF-𝜅B expression was reduced by GEHS. Conclusions : These results suggest the clinical basis of GEHS for the treatment of inflammatory diseases.

Functions of Early Palindrome Domain in SV40 DNA replication

김연수,하정실,강현삼,Kim, Yeon-Soo,Ha, Jeong-Sil,Kang, Hyen-Sam 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.3

SV40 Replication core origin에 존재하는 early palindrome의 구조와 기능에 대해 알아보기 위해 SV40 origin을 포함하는 0.8kb DNA fragment를 filamentous phage M13 DNA에 cloning시킨 후 oligonucleotide-directed site-specific mutagenesis에 의해 early palindrome이 이룰 수 있는 hairpin 구조 중 stem 부분이 파괴된 mutant들과 base의 치환은 있으나 stem 부분은 다시 회복된 mutant들을 만들었다 in vivo replication assay를 위해 mutation이 일어난 origin 부위를 pAT153 vector로 subcloning하여 COS-1 cell에 transfection함으로써 replication level을 측정하였다. 그 결과 early domain의 inverted repeats는 fuctional cruciform structure를 형성함으로써 SV40 replication에 관여하는 것은 아니라는 것을 알 수 있었다. To define the roles of the early palindrome domain within the SV40 replication origin region involved in initiation of DNA synthesis, 800-bp DNA fragment containing the simian virus 40(SV40) origin of replication was inserted into filamentous phage M13 DNA. Four kinds of mutants were introduced into this recombinant phage DNA, M13SV-2, by oligonucleotide-directed site-specific mutagenesis. Then, the origin fragments containing one or two base substitutions were subcloned into pAT153 vector, a derivative of pBR322. In two mutants (pATSV-A,-B) the stems were destroyed by single base substitutions. In the other two mutants(pATSV-C,-D) the normal stems were restored by additional base substitutions which complements the bases altered initially. While pATSV-A and pATSV-C could tolerate base substitutions, pATSV-B and pATSV-D showed dramatic decrease in DNA replication.

치안분야의 정보통신기술 활용방안 연구 - 빅데이터기반 치안수요분석과 대응체계를 중심으로 -

김연수,Kim, Yeon Soo 한국융합보안학회 2016 융합보안 논문지 Vol.16 No.6

급격한 정보통신기술의 발달은 경찰에게 새로운 도전이자 기회가 되고 있다. 이 연구는 국내외 치안분야의 ICT기술 활용사례를 검토하고, 그 시사점을 확인하는 것을 목적으로 한다. 이를 위해 정보통신기술 활용에 관한 경찰활동의 근거이론으로 정보주도 경찰활동(intelligence-led policing), 예측적 경찰활동(predictive policing), 증거기반 경찰활동(evidence-based policing)의 개념에 대해 소개하였다. 또, 빅데이터 기반 범죄분석과 예측기술 활용방안과 경찰의 정보통신체계 및 지휘통제기술의 고도화방안에 대해 논의하였다. 이 연구를 통해 확인한 시사점으로 (1) 기본데이터의 확보, (2) 활용가능한 통합D/B의 구축, (3) 정책결정자의 활용성 증대, (4) 유관기관 사이의 교류협력, (5) 전문가집단의 양성, (6) 통합 D/B구축의 법적 근거와 실무적 가이드라인 마련을 제안한다. Rapid advances in information and communications technology are new challenges and also opportunities for the police. For the purpose of identifying its implications, this study reviews utilization cases of information and communications technology in the field of public security in South Korea and other countries. As theoretical basis for utilization of information and communications technology, this study introduces intelligence-led policing, predictive policing and evidence-based policing. Also, utilization of big-data based crime analysis and crime prediction technology, as well as advancement of information and communications system and command and control technology of the police, are discussed. Based on the identified implications in this study, the following proposals are made. They are (1) procuring basic data, (2) creating an integrated database, (3) increasing utilization of policy decision-makers, (4) exchange and cooperation between related institutions, (5) training professional analyzers, (6) establishing legal basis and practical guidelines for an integrated database.

좌측 주기관지 근위부에서 발생한 무기폐를 동반한 횡문근 육종의 수술 치료 -1례 보고-

김연수,장우익,허진원,박시영,장선희,박경택,김창영,류지윤,조성준,Kim, Yeon-Soo,Chang, Woo-Ik,Huh, Jin-Won,Park, See-Young,Chang, Sun-Hee,Park, Kyung-Taek,Kim, Chang-Young,Ryoo, Ji-Yoon,Cho, Seong-Joon 대한기관식도과학회 2007 大韓氣管食道科學會誌 Vol.13 No.2

Treatment choice for primary pulmonary sarcoma is complete surgical resection. A 69 year old man developed dyspnea due to left lung atelectasis. There was endobronchial tumor completely obstructing the left main bronchus. The tumor was resected completely by main bronchial resection via a left thoracotomy incision, and diagnosed as leiomyosarcoma. Bronchoscopy and computed tomography in 6 months after operation, there was no evidence of recurrence.

Surgical Treatment of Mediastinal Schwannoma Showing Malignant Potentiality on Chest Magnetic Resonance Imaging Study : A Case Report

김연수,박경택,류지윤,김창영,장우익,장선희,Kim, Yeon-Soo,Park, Kyoung-Taek,Ryoo, Ji-Yoon,Kim, Chang-Young,Chang, Woo-Ik,Chang, Sun-Hee The Korean Bronchoesophagological Society 2008 大韓氣管食道科學會誌 Vol.14 No.2

Nerogenic tumor of various histologic types may arise in the posterior mediastinum. Mediastinal schwannoma is a frequent paraspinal neurogenic tumor, but malignant mediastinal schwannoma is rare tumor which is derived from Schwann cells. Although there are some reports dealing with approach for screening patients with symptoms suggesting malignancy and the imaging criteria for distinguishing malignant from benign schwannoma but the results are not clearly defined. We present a case of hugh mediastinal schwannoma which was taken for malignancy in imaging studies because of its invasiveness.